ISAma4

  • Family IS66
  • Group ISBst12
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_011138 ND Alteromonas macleodii
Alteromonas macleodii 'Deep ecotype'
DNA section
IS Length : 1529 bp

Ends


IR Length : 28/29

IRL : GTAACCGTTCACCAGGGCCCTATTGACAGTTAGTAATATTTATTTTCAAT
IRR : GTAACCGTTCACCAAGGCCCTATTGACAGGCAGCTTTGAAGTCGTCGAGC

Insertion site


Left flankDirect repeatRight flankDR Length
TTTTCCACTCCAATATAGAGACTTTTAG8
GAAGCACCTAGAATAGTCCCTCTCACAAAG9
CGCTCTGGCTGTTTCGCCAACCACTAACAC8

DNA sequence

GTAACCGTTCACCAGGGCCCTATTGACAGTTAGTAATATTTATTTTCAATGATCGTGGGGATCCTATTGATTGATCCCGCCAGTCTGTCAGAGTGGTTTC
ATGCCACGTAAAAAGCAGCCCACAAAGCCACCTGTCGCTACTAATCTCGATGATGCCAATGAGCTCATTAGTACACTCTGGGACAGGCTAAATGACCTCG
AAGACCGACTGAATCAAAACAGTCGTAATTCATCGCGCCCGCCATCGTCTAATGGGCCGGGGGCGTCCTCTTCTGCGCCAGCTAAAAAGCCGACAGGCCG
TAAGCGCGGTGCGCAATCCGGCCATAAAGGCAGTAAGCGGATGCTGGCCGATACCGTTGATGAGACGCGCACCTATTATCCCGACGATACTTGTGCTTGC
GGTGGTACTATTACTATCAGTGATTCACCGTATCGCCGCCATCAGGTTTTTGATATTCCTTCTCAGGCTTTCTCTGTGGTTGAACATCAGTTACATCAGG
GTCAGTGTTGTCAGTGCAGCAAAACGGTAAAAGCAACGCTACCTGACAACGTGAATCAGGGACAGATGGGCAATAACCTGCTGGCATATGTGGCAATGCA
GTCCGGTCAGTTTCACCAGAGTATCAGTAAAATCCAGCAACAGCTCGAACAGAACTTCGGCTTATCGTTCAGTCGCGGTGCTATCTCTGAAGCGCAGGGC
CGGGTTAGTTCAGTACTGACGCCAGCGTATCAGGATATCAAAGAGATGATGCGCACTGAGGCGGTGGTACACTGCGATGAAACCCGTCATCAGCGTGGTA
ACGAAAACCGCTGGATGTGGCAGGTATGCACAGCAGAATTATCATGCTTTATGACGCACTTCTCACGTGGCGCCTGGGCGGCAAAGAAACTACTCGGCGA
TAATCCGGAAAACATTGTTGTTACTGACCAGTATGCCGGTTATCACTACATCGATTCAGACCACCGTCAATTGTGCTGGGCGCATATCTTAAGAAACATG
AATGCGCTGGCGGAAAGCTGGGGTACGAACAAGACTTATGGAACGACGTTAGTCAGGCTCATTCGCATACTGTTCCGGCTGCAACACCGGTATGAGAGTA
ACGCACTAAGTGAAAAGCGATACCTGGACCGGATGGAAAAACTACGCATCGCCTGGCGAGAGCAACTTGAACTGGCATCAAGACGCTGTGTGACTCCCCG
CTATCAGAACCGGTGTAAGCTACTGCTAAAACACGATGACATGTGCTGGGTATTCCTCAGTCACGATGGCGTCCCTCTCACTAACAATGAAGCCGAGCGT
TCATTGCGAAGCTATGTGCTGTGGCGAAAAGGCAGCTATGGTGTGTGGTCTCACCGAGGGGAACTGTTCAGACAACGTATACTCACCATCGTCGAAACCT
GCCGGAAACAAAAGCTGAATCCACTGAATTGGCTCCGCGCCATTCTCGAAGCGACGCTGAACAAAACGTCCTATCCTCTGCTCGACGACTTCAAAGCTGC
CTGTCAATAGGGCCTTGGTGAACGGTTAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1410 bp469 aa1011510+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRKKQPTKPPVATNLDDANELISTLWDRLNDLEDRLNQNSRNSSRPPSSNGPGASSSAPAKKPTGRKRGAQSGHKGSKRMLADTVDETRTYYPDDTCAC
GGTITISDSPYRRHQVFDIPSQAFSVVEHQLHQGQCCQCSKTVKATLPDNVNQGQMGNNLLAYVAMQSGQFHQSISKIQQQLEQNFGLSFSRGAISEAQG
RVSSVLTPAYQDIKEMMRTEAVVHCDETRHQRGNENRWMWQVCTAELSCFMTHFSRGAWAAKKLLGDNPENIVVTDQYAGYHYIDSDHRQLCWAHILRNM
NALAESWGTNKTYGTTLVRLIRILFRLQHRYESNALSEKRYLDRMEKLRIAWREQLELASRRCVTPRYQNRCKLLLKHDDMCWVFLSHDGVPLTNNEAER
SLRSYVLWRKGSYGVWSHRGELFRQRILTIVETCRKQKLNPLNWLRAILEATLNKTSYPLLDDFKAACQ

 

Blast result :
Comments
ISAma4 is 63% aa similar to ISVch7.
References
1] ISfinder annotation (2008).

2] Ivars-Martinez,E., Martin-Cuadrado,A.B., D'Auria,G., Mira,A., Ferriera,S., Johnson,J., Friedman,R. and Rodriguez-Valera,F. (2008) ISME J 2 (12), 1194-1212.