ISAcma4

  • Family IS1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017 plasmid pREB3
Acaryochloris marina MBIC11017 plasmid pREB4
Acaryochloris marina MBIC11017
DNA section
IS Length : 796 bp

Ends


IR Length : 24/25

IRL : GGCGTTGCTCAATGACGGGATGAATTAATTGGATTGCGTCAGGATAACCT
IRR : GGCGTTGCTCAATGACGGGATGACTCACGCAGGAATAGGTACTTCCCCAA

Insertion site


Left flankDirect repeatRight flankDR Length
TTTAACTAGGCACCAGCTAAATTGAAACCCATAAAC8
AAGACTTAATGACTTTTCTTGAGTATTTAGAGGGAA8

DNA sequence

GGCGTTGCTCAATGACGGGATGAATTAATTGGATTGCGTCAGGATAACCTCATGACTATTTCAAACATCATGAGGAATCTGTATGAAGTGTCCTGAGTGT
GGTGGTCAACATATCCATAAGAATGGGCATCGTCGAGGTAAACAAAATCATATCTGTGTGACCTGTGGACGACAGTTTCTTTCAACTTACTCAAAGCGTG
GCTACAGTGATTGGACGAAACGACTCTGCCTGAGAATGTATGTCAATGGAATGGGGTTGAAGGGTATCGAACGAGTCATAGGCGTTGCTCATACCACTGT
GATTCATTGGGTTCAACAAGTTGGAGTTCTATTACCCGATGCTTATGACCCCGACGATATCCCACAGGTCGGAGAATTAGATGAACTCGAAACCTTTGTG
GGCAAAAAACGCAATAAGGTCTGGATTTGGACTGTGGTGGATCATTTTCACCCCGGCATTCTAGGCTGGGTTGTGGGTGACCATAGTGCTGAGACCTTTC
GACCGTTATGGCAAGCAATCGTCTGTTGGCAATGTTTTTTCTGGGTCAGTGATGGCAATCCCGTCTATCCAGGCTTTATCCCAGAGGGTGACCAGATTGT
TAGCAAGACCTACATGACACGAGTAGAGGGAGAAAATACTCGTTTACGACATTACTTGGCACGGCTCCATCGCAAGACTTTATGTTATTCAAAGTCGGTG
GACATGCTCAAGCATTCAATTCGACTATTGATCCACTATCTCAAGTTTGGGGAAGTACCTATTCCTGCGTGAGTCATCCCGTCATTGAGCAACGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
690 bp229 aa83772+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKCPECGGQHIHKNGHRRGKQNHICVTCGRQFLSTYSKRGYSDWTKRLCLRMYVNGMGLKGIERVIGVAHTTVIHWVQQVGVLLPDAYDPDDIPQVGELD
ELETFVGKKRNKVWIWTVVDHFHPGILGWVVGDHSAETFRPLWQAIVCWQCFFWVSDGNPVYPGFIPEGDQIVSKTYMTRVEGENTRLRHYLARLHRKTL
CYSKSVDMLKHSIRLLIHYLKFGEVPIPA

 

Blast result :
Comments
ISAcma4 is 83% aa similar to ISMae3.
There is no expected frameshift in this IS.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C., Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S., Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.