ISAs8
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009348 | ND | Aeromonas salmonicida | Aeromonas salmonicida subsp. salmonicida A449 |
DNA section
IS Length : 754 bp
Ends
IR Length : 20/22
IRL : GGTGATGCCTCCATCGGTTGATGCGGCATGGTGGGTTCCTTTGAGGCTCA
IRR : GGTGATGCCTCCAATCAGTTGAAGTGATGGTTGTCGATGAAGGCGCCGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAGCTGCTGGTGCAGCAGGC | CGTTACCAGTTCTTCGATGT | 0 |
DNA sequence
GGTGATGCCTCCATCGGTTGATGCGGCATGGTGGGTTCCTTTGAGGCTCAGCAATTCATGGTATACATCACCTCACTGTCCTCGTTTCAACTCCGACCAC
GTATATCGCCACGGTAAGACTCCTGCCGGTCACGTTCGCTATCGTTGTCCAGCCTGCCCCCACGTTTTTCAGCTCACCAACACCTATGAGGCCCGTAAAC
TCGGCGTTAAAGAGAAGATTGTTGATATGGCGTTCAACGGGGCTGGTGTCCGTGATACTGCTCGCGCCCTTAACACTGGCATTAACACCGTCCTGCGCGC
CCTAAAAAACTCGCCCCAAGGCAGTAACCTCCGAAAAGGTAGTGCTGGATGATGTCGCGCTTATCTGTGAACTCGATGATCAATGGGCGTATGTAGGTAA
CAAAAATAATCGCCACTGGCGTTGGGATACCAAAAGAAAACGTGTTGTTGCCTACACCTTCGGCCCGCGCAACGATGAAAAATGCCGTAAACTGCTGAGC
CTGTTGGCCCCTTTTCAGTTTGGCTTTATCACCAGTGATAACTGGGATAGCTACGCCAGAGAAGTCCCCTGCAAGATACATCTGACTGGGAAAATCTTCA
CGCAACGTATCGAGCGTAATAACCTGACGCTTAGAACGCGCCTCAAGCGACTAGCTCGCAAAACCATCTGCTTTTCTGGCTCTGTTGAGCTCCACGATAA
AGTCATCGGCGCCTTCATCGACAACCATCACTTCAACTGATTGGAGGCATCACC
GTATATCGCCACGGTAAGACTCCTGCCGGTCACGTTCGCTATCGTTGTCCAGCCTGCCCCCACGTTTTTCAGCTCACCAACACCTATGAGGCCCGTAAAC
TCGGCGTTAAAGAGAAGATTGTTGATATGGCGTTCAACGGGGCTGGTGTCCGTGATACTGCTCGCGCCCTTAACACTGGCATTAACACCGTCCTGCGCGC
CCTAAAAAACTCGCCCCAAGGCAGTAACCTCCGAAAAGGTAGTGCTGGATGATGTCGCGCTTATCTGTGAACTCGATGATCAATGGGCGTATGTAGGTAA
CAAAAATAATCGCCACTGGCGTTGGGATACCAAAAGAAAACGTGTTGTTGCCTACACCTTCGGCCCGCGCAACGATGAAAAATGCCGTAAACTGCTGAGC
CTGTTGGCCCCTTTTCAGTTTGGCTTTATCACCAGTGATAACTGGGATAGCTACGCCAGAGAAGTCCCCTGCAAGATACATCTGACTGGGAAAATCTTCA
CGCAACGTATCGAGCGTAATAACCTGACGCTTAGAACGCGCCTCAAGCGACTAGCTCGCAAAACCATCTGCTTTTCTGGCTCTGTTGAGCTCCACGATAA
AGTCATCGGCGCCTTCATCGACAACCATCACTTCAACTGATTGGAGGCATCACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 35 | 352 | + | No |
Description : First part of the transposase
ORF sequence :
VPLRLSNSWYTSPHCPRFNSDHVYRHGKTPAGHVRYRCPACPHVFQLTNTYEARKLGVKEKIVDMAFNGAGVRDTARALNTGINTVLRALKNSPQGSNLR
KGSAG
KGSAG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 435 bp | 144 aa | 306 | 740 | + | No |
Description : Second part of the transposase
ORF sequence :
KTRPKAVTSEKVVLDDVALICELDDQWAYVGNKNNRHWRWDTKRKRVVAYTFGPRNDEKCRKLLSLLAPFQFGFITSDNWDSYAREVPCKIHLTGKIFTQ
RIERNNLTLRTRLKRLARKTICFSGSVELHDKVIGAFIDNHHFN
RIERNNLTLRTRLKRLARKTICFSGSVELHDKVIGAFIDNHHFN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 706 bp | 234 aa | 35 | 740 | + | Yes |
Chemistry : DDE
ORF sequence :
VPLRLSNSWYTSPHCPRFNSDHVYRHGKTPAGHVRYRCPACPHVFQLTNTYEARKLGVKEKIVDMAFNGAGVRDTARALNTGINTVLRALKNSPQGSNLR
KVVLDDVALICELDDQWAYVGNKNNRHWRWDTKRKRVVAYTFGPRNDEKCRKLLSLLAPFQFGFITSDNWDSYAREVPCKIHLTGKIFTQRIERNNLTLR
TRLKRLARKTICFSGSVELHDKVIGAFIDNHHFN
KVVLDDVALICELDDQWAYVGNKNNRHWRWDTKRKRVVAYTFGPRNDEKCRKLLSLLAPFQFGFITSDNWDSYAREVPCKIHLTGKIFTQRIERNNLTLR
TRLKRLARKTICFSGSVELHDKVIGAFIDNHHFN
Blast result :
Comments
ISAs8 is 75% aa similar to IS1N.
References
1] Reith,M.E., Singh,R.K., Curtis,B., Boyd,J., Bouevitch,A., Kimball,J., Munholland,J., Murphy,C., Sarty,D., Williams,J., Nash,J., Johnson,S. and Brown,L. (2007) Direct submission GenBank.