ISAzca1

  • Family IS1595
  • Group ISNha5
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009937 ND Azorhizobium caulinodans
Azorhizobium caulinodans ORS 571
DNA section
IS Length : 4520 bp

Ends


IR Length : 24/25

IRL : CGGCATTAGGTAGCAAACTCACCTAGTGCCTGCCACGCCATCAAGGCGGT
IRR : CGGTATTAGGTAGCAAACTCACCTATAAAAAATGATGTCAACGCAATGCG

Insertion site


Left flankDirect repeatRight flankDR Length
GGACGCGGCGGTGGGTGGAAAATTGCTGCAATGCGGCAGTTG8

DNA sequence

CGGCATTAGGTAGCAAACTCACCTAGTGCCTGCCACGCCATCAAGGCGGTTGATACGCTCCATTGATGCTCCACAATCGCGCTTGCAGACAGCCGCGATT
CAGTGCAAGGTGCATACATGCCGTCATTCAACCCAACTCTCTCCGATGTCCTCGCGCGCCGCGGCGAAGTCAAGCGCTTGATCGATGCCCTCAAAACTGA
GGACGCCGAGCTTGAAATGGCAGAGCGCGTTCTAAAGCGCCTTGGGCACCCGCAGGGTGTGTTTGACGCTCGCGACTATGAGCCAAGTCAGACCAGTGCG
GTGCAGCCCCAAGAGGTTACGCTTACCGGGAAAGAGACGACCGAGGAGGTTTTGATCCACCTACTCTCTGGAGCGCAACTTTGGTGGACTGCGAACCAAT
TGCAGGAAGCAGCATCCCGCGCTCTGGGGCGGGAAGTCCCGATGACAACCGTTTCCCCCACTCTTTCCCTTATGAAAGGGAAAAAGTTGATTTCTCGCGA
CGGCCTTAAGGTTGCGCTTGCGTCTCGCGTAACTGGATTGACCGAAGACGATTTGAAGGATGCAGGGATTATCCCTCCGCACGCTACTCTGCGTAGCCCG
CCAGAAGGCGGCTCACAAGGCGGCGGGGTGATGGGGTAATAAAAAAGCGGCTGCGCCCTAAAGTGCAACCGCTTTTTGAACGAGTGTGCCACCCACGTTC
TTGGCGGAGCCGGGTGACGCAAAACCCACAAACAAGGAGGCGGTAAGGGCCGCTAAGCCTGTGGTCTTATTAGATTGACGAAGTCCCGCCGGTTGACACT
GCGGTAGCAGTGGTGCCCCGGCGGGCACCCCATCTGCGGGGCTAGCATAGTGCGGGGTCCGGCTTTCCATCCAAGTCCCCGAGGGTGCAAGTCCCTCTAG
CTCCACCATCGGCGCACTGGGCAAAAGCTCGGTGCGCCGACCTATTTAAATTAGTGGAATCTCTGGATTCCGTCCAGTTGCAATTAATTGCACTGAGCCT
GGGATGCTATGCCCGATGACCCTTCGCGGTCGAAAAATGAAATTCCCTGTTTTTCCAGAGCAGCTCTCATTGCCGATAGATTGTTTGCTATCGGAACGCG
GCGCCCCTTCTCAAAGTCGCGGACGGTCGATAGGGAAACGCTGGCCGCGCGCGCAAGATCGTCTTGCGACCAGTCAATCCAAGCCCTTGCTGCACGGCAC
TGTTCAGGCGTCATCCGTTCCGCTTAAGCGCGAAATCATTTTTGGTCAACCCCAACGTTTTTCGTTGACGCCAACTCTTTACGCCATTATCACTGAGGTC
AACGCAAATCGTTGGCGCGGAAATGGCTCAGCACTTCCTTCTCTCACGCGCTGCCCGGACTATCAGTCTGGCGAAGGTCGCGCGTATGAGCGACGAGGAA
GCCCACGACGCCTTCCGCAACATCCGTTGGGCGGCCACTGACGGCAAGCCGGTTTGCCCGCGTTGCGCCTGCGCCGAAACCCTCACCTACAAGACCCGCA
AGCTCTATAAGTGCAAGGCTTGCGCGCATCAGTTCTCGGTCACGTTGGGCACCATCTTCGCCAGCCGCAAGCTGCCGATCCGCGACTACCTCCTTGCTAT
CGCCATCTTCGTCAACGGCGCCAAGGGCCATAGCGCCCTCCAGCTTTCCCGCGATCTCGACGTGCAGTACAAGACGGCTTTCGTCATGGCTCACAAGATC
CGCGAAGCGCTTGCGGCGGAAATGAAGGACATGACAGTTTCCGGCACGGTTGAGGTGGATGGCGCCTATTTCGGCGGCCACATTCGCCGAGAGAACCGCA
AGGAAGACCGCAAGGACCGCCGCCTAGCCCAACACCAGACCGGCAAGCGCCGCGTTGTGGTCATCATGCGTGAGCGCGGCGGGCGGACGCTCCCCTTCGT
GTTTAAGGCCGAAGCGCATGCAATCGAAACGATTGCCCAGCGCGTGGCGCCCGGCTCGATCATCCATGCCGATGAAGCCGCCCATTGGGACAAACTGCAC
GCGCTATACCTGACCAAGCGCATCAACCATCAGGAGAGCTATTCAGACGGCGAAGCCTGCACCAACGGGGCGGAAAGCTTCTTCTCCCGCATCCGCCGCG
CTGAGATCGGCATTCACCACCACATCAGCGGCCCGTACCTCGCTGCGTATTCCGCCGAAATGGCGTGGCGTGAGGATCATCGCCGGGTGAGCAATGGCGA
GCCAGTACCTGATGACGGTCGGCTCCGCTCTGGCTCACCCGGTGAGCCGAGTGTGGAAGGGCTACTGGCAGCGGTGACTTTAGGCCGTTCTCGACCTTGG
AGTGTGCGCCCTCACAATCTTGGCATCGCGAATTTCTTCCCCGACCCTTCGGGCGTTGACGTGAACCACTACGGGTTTTTGACTCCACTCCGCACGTTCA
ATTGCCTTTTGGTATTTGGCTCCGACCAATGGGTCTTCCAAGATCGCAGTAAACCTATCTTCACCATCAACAGACTCTAGGCGCACTTGAAAAGCCGAGG
TCTGCTCTGTATCGACACCCCTGACATAAAACTGTTTTTTTATTACAATTTTAGACGACTTCGATCTGGTTGATCTGGTTAGAATAGAAATTGAACTAGA
ATCAAATACAGCACCTTGAATATTTAGCTCGTCAACAGATGTGGAATTCTTTATTATCTCCGACATTGCATGGTTGCTATGCTCATAAATCTTCGCCGCT
ATAGGAGATTTTCGCTCCGCCTTCTTTAAGGTAGTGCTCTCCGTTCTCCTATCATTAATTACTTTTTCTAAAAATTCATATAAATCTTTCTTTTCCTTTC
CATTTGATTCAATTTGTTTTTCCTGAAGTTCAATCTTCTTCATTTCAAGCTGCGAGTTTATGTATGCCGTCACGCCTGCTTGCGTGAAGTACACGAGAAT
GGCGACGATAAAGCCTATCAGGATTTGGCGGCCAGTCATTTTATCTATCGCCTTCTCTCCGATGCCCTTTATTTGCTTTTCTGCGAACGCCCAAAAATCG
GAACTTCCCTTTTTTAAGGTAAAAATTAATTCTAGATCTTCTTTTTCCTGGTCAGTTAGTCGCGTGACTCGATCATCATCCTTGAGTATCAGAGCCGCCG
TTCTGTAGAAGTCATTCTGAAACTCAACTAACGACTTCATCACTGAAGTTGGAATAGTCCCATCAAACGAATTACCCTTATATATAAGGCGAAGCCCAGC
CCAAGACATGTCGTCTAGGTCCAGAATTACATTCTTGGCGTCAATGGTTTTATCAAGAAGTGCATTTAGCGCATTCCAAGCCGCCTGCTCGCTATCCAAA
CGCAGGACGACAGCCCCCTCTCCCACAGCCATCTAAGCTCCCCCCGGTCTTTCGTTGGGAACTTATATCCGCGTGCCAGTAGGTCAAGCTTGGGTTGTAT
CAACTTAGGTTAATCGCCAGCACATTGTGACGCCGACCTTAAGTGCCAGAGACGAACGCCACGTCGGGTGCCGGACTCAAGCAATCCACGTCTTGCTTGA
AGCCTCAACGCCTGCACGATTCTAAGCGCTATCGTCTGCCGCAGCGTCTTGTCGCTCTCATCCAGACCCTTGACGCTGATGACGCGCAGCGCCAGTTCCC
GCGTGTCCAGAGGTCCTTCCTGTACCAGCGCAGCCTTGCATATGGCTGTCATTTCGCCTCTCTTAAAGAGGCGATGAAGCTCCACATGGGCCGGGAACTC
TCTGGGCTCCCCGTTGACTTCGAAGATGCAGATGGTGGCGTTCACGGACATGAGGTCGCGCTTCGCGTCCGCTATCTGGCGTTCGAGCTTGCCTATGTAG
GCTTCGATTTCATCGCGCTTGCGTCTCAAGGTAGACAGAACCTGAGGCTCAACCATAAGCTTCTCCACAACCAGAGGGCGGGGAAGCGGGCGATGTACAA
GAGCGAGACGTTCCGGCTGTTTGAGCGCGCAATCGAAGAGAAAAAGCAAATCGTGTGCACATACCGAGGGTACCCACGCGAGATTTGCCCTCATGCTGTC
GGGTGGTCAGACAATGGAGAGCAAGCGCTTGCATTCCAGTTCGCCGGAGGTAGCTCAACGGGGCTCCCCTTTGGTGGAGAGTGGAGGTGTTTTGAACTAA
GGCATCTTCAAGGCGCCGTCTCCGAGATGGCGCTTGGCACACCGGAGATAGTCATAAAAAACCTCAAACCTGCGTAAAGAACGTGGCGCTAGAGGTGGAT
CACTGAACGCCGTAGGCAAGCGTGGCGTAATCCCTTGCCCGCTGCTTGGCCTCAGGGCCGACGAAGAACGATTGATAAATCTCTCCGTCCGTCCCGATCG
CTTCGACGCTCCAAACGCCGGGATCGTCGGTCCGTTCGAAAACCTCAGCGCGATTAATGGGCTCATGGCGTGGTAGATATTCCATTCCCCGCCTCCTTCT
GTTCCATGGGGGAAACGGCGATTGGCCAGGGGCGTTCCTAACCCCATTCATATCAGATCGTTTACGCCAACGCATTGCGTTGACATCATTTTTTATAGGT
GAGTTTGCTACCTAATACCG
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
231 bp76 aa9841214-No
ORF function : Passenger Gene
Annotation : Transcriptional regulatorDescription : Transcriptional Regulator factor

ORF sequence :

MTPEQCRAARAWIDWSQDDLARAASVSLSTVRDFEKGRRVPIANNLSAMRAALEKQGISFFDREGSSGIASQAQCN

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1158 bp385 aa13232480+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAQHFLLSRAARTISLAKVARMSDEEAHDAFRNIRWAATDGKPVCPRCACAETLTYKTRKLYKCKACAHQFSVTLGTIFASRKLPIRDYLLAIAIFVNGA
KGHSALQLSRDLDVQYKTAFVMAHKIREALAAEMKDMTVSGTVEVDGAYFGGHIRRENRKEDRKDRRLAQHQTGKRRVVVIMRERGGRTLPFVFKAEAHA
IETIAQRVAPGSIIHADEAAHWDKLHALYLTKRINHQESYSDGEACTNGAESFFSRIRRAEIGIHHHISGPYLAAYSAEMAWREDHRRVSNGEPVPDDGR
LRSGSPGEPSVEGLLAAVTLGRSRPWSVRPHNLGIANFFPDPSGVDVNHYGFLTPLRTFNCLLVFGSDQWVFQDRSKPIFTINRL

 

Blast result :
Comments
ISAzca1 is 71% aa similar to ISAusp1. The transposase is the second ORF, the first is a passenger gene annotated as transcriptional regulator.
References
1] Suzuki,S., Aono,T., Lee,K.B., Suzuki,T., Liu,C.T., Miwa,H., Wakao,S., Iki,T. and Oyaizu,H. (2007) Appl. Environ. Microbiol. 73 (20), 6650-6659