ISRme9

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006525 ND Ralstonia metallidurans
Ralstonia metallidurans CH34 plasmid pMOL28
DNA section
IS Length : 2688 bp

Ends


IR Length : 39/47

IRL : TGCGTATTTCGGGGCGCGTGATCAGCGATTTCGGGGGAACGTGATCACCT
IRR : TGCGGATTTCGGTGAAGGTGATCAGCGGTTTCGGCGAACGTGATCAGGGA

Insertion site


Left flankDirect repeatRight flankDR Length
GCGACATTCCCCTTCCCCGCCGCATCGTAG2

DNA sequence

TGCGTATTTCGGGGCGCGTGATCAGCGATTTCGGGGGAACGTGATCACCTGTTTCGGGCGGATGCTGATCGCTGGTTTCGGCGGAACGTGATCACTTCGG
CGATGCCGCCGAAATCGGTGATCAGCTTCCCGAAACGAGTGATCACGTTGCCGAAATAGGTGATCAAGCAGCCGAAATGGGTGATCACGCACCGCAATGG
GGCCGGGGTTAGGGGTGGGACTGCGCATGGACTAGAACGCCGGCGATACGCTGCTTCCTTTTTCAAGGAAGCAAGCTGATGCCGGCCAACCGGATGACCA
TGCGCAAAATCAAGGAAGTGCTTCGCCTGAAGTGGGCGTGTGGCCTTTCGCACCGGCAGATCGCTAGGGCGACGGGTGTGAGTGTTGGCGCCGTTTCGCA
GTACGCGGCAATGGCGAAGGCAGCGGGGCTGGATTGGCCGCAGGCGGACAGCCTGAACGAAGATACGCTGGAGCAGCGGCTGTTCGGCGCGCAGAAGCCG
GCCAACAGCCCGGGCGGGCGGGTGATGCCGGACTTCCCCTATCTGCATCGCGAGTTGCGCCGCAAGGGCGTGACACTGCAGTTGCTGTGGGAGGAATACC
TGGAGGCGAATCCAAACGTCCCCATTTACCAATACACGCAGTTCTGCTGCCGTTACCGGGACTGGGCAGCGACGCTCAAGCGGTCCATGCGCCAGCAGCA
CCGCGCCGGCGAGAAGCTGTTTGCTGACTTCGCCGGTCAGATGGTGCCGATTCTGGATGCGGAGGGCGGCATTGCCTTCGAAGCCAGCATCTTCGTCGCT
GTGCTCGGCGCTTCAAATTACACGTACGCGTGCGCCACCCGGGGGCAGACCACTGCCGACTGGATCGGGGGAATGGTCTGCGCGATGGAATTCGCCGGCG
GCGTGCCCGAGCTGCTTGTTCCGGACAACCCGCGCGCGCTGGTGGCTCGCCCTGACCGATACGAGCCAGTGCTGTCCCGAACTGCAGAAGACTTCGTTCA
TCACTACGGCACGGCCATGCTGCCAGCACGGCCACGCAAGCCGCAAGACAAGGCCAAGGTCGAGACTGGCGTACTGATCGTCGAGCGGTGGATCCTAGCG
CGGCTGCGCAATCACCGCTTCTACAGCTTGGGCGAGCTCAACAAGGCTATCAAGAAGCTCGTCGCCGACCTGAACGACCGACCGTTCAAGAAGCTGCCTG
GCACGCGCCGGGAGTGGTTCGAGCGGTTGGACCGGCCAGTGCTGCGACCGCTGCCGCCGCGGCGCTACGAAGTTGCAACGTTCAAACAGTGCCGCGTCAA
CGTCGACTACCACGTCGAGATCGACGGCCACTACTACAGCGTGCCGCATGCCCTGGTGCGCAAGGCGGTGGAGGCTCGCGTAACTCGCCACACCATCGAG
ATCCTGTACGGCGGCAAACGGGTGGCGCTGCACGCGCGCAATACCCGCAAGGGCAGCCACAGCACGGTCAAGGAACACATGCCGGTGGCCCACCGCGCGC
ACCTCGAATGGACGCCCGGCCGGCTGCTCAACTGGGCAGCCTCGATTGGGCCCAACGTCGCCGTCATCGTCGAATACCAGCTCACCCACAAGAGCCATCC
CGAGATGGGCTATCGCGCCTGCCTGGGTCTGCTGAGTCTGGCCAAGAAGTACAGCAAGGAGCGGCTCGAAGCCGCTTGCGCCCGCGCCGTCGCCATTGGC
TCGCTCACGCGTAAGTCTGTGGTCTCCATCCTTGAAAACCATCTGGACCGGCAAGCCACTCTGCCGGTATCGCAAACCGAGTGGCATTCCCCCATGCACG
ACAACGTGCGCGGACCCGACTACTACCATTAGGAAAACTATGCTGATGCAACACACCGTCGGCCAGCTCAAGGCCCTGAAGCTCGACGGGATGGCGCGGG
CCTTCGAGGAACAGTCTGCCCTGACCGCCAGCTCCAGCCTGCCGTTCGAAGAACGCTTCTCCATGCTGGTCGACCGGGAGATTGCTTGGCGTGACACCAG
GCGCCTGGAGCGGCTGCTGCGCTCGGCCAAGCTCAAGCACGCCCAGGCATGCCTCGAGGATGTGGTCTATGACGGCAGCCGCGGTCTTGACCAGCGGCTG
GTCGCCAGCCTGGCCAGTTGCGACTGGATCCGCAATGCTCAGAGTCTCATCCTTACCGGGGCGACCGGTGCCGGCAAGTCCTGGCTGGCCTGTGCGTTTG
CTCAGCAGGCCTGTCGGCAGGGATTCTCTGCGCTCTACCTGCGGGTGCCGCGACTGTTCGAGGAACTGCAGATCGCCCACGGCGACGGGAGCTTCACCCG
CCGCCTGGCACAGCTCGCGCGCATCGATGTCCTGGTGCTGGACGACTGGGGCCTACAGGAGCCTTCTAAAAGCGCACGCGGCGATCTGCTGGAAGTTCTG
GACGATCGCGTCGGCACCCGCTCGACCATCGTGACCAGCCAGCTCCCGATCGAGCATTGGCACCAGTGGTTGGATGATCCGACGCTGGCTGACGCCATTC
TGGACCGGCTGGTCCACCAGGCGCACAAGGTATCGCTCAAAGGCGAATCCATGCGCAAGCGCTCGGCTACCGACGTCAAGAAACGGGCATCGTGATCACC
TACGCTACAATCTCCTGACCGCGCAGTACCACCTTCCTTCCCTGATCACGTTCGCCGAAACCGCTGATCACCTTCACCGAAATCCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1554 bp517 aa2791832+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPANRMTMRKIKEVLRLKWACGLSHRQIARATGVSVGAVSQYAAMAKAAGLDWPQADSLNEDTLEQRLFGAQKPANSPGGRVMPDFPYLHRELRRKGVTL
QLLWEEYLEANPNVPIYQYTQFCCRYRDWAATLKRSMRQQHRAGEKLFADFAGQMVPILDAEGGIAFEASIFVAVLGASNYTYACATRGQTTADWIGGMV
CAMEFAGGVPELLVPDNPRALVARPDRYEPVLSRTAEDFVHHYGTAMLPARPRKPQDKAKVETGVLIVERWILARLRNHRFYSLGELNKAIKKLVADLND
RPFKKLPGTRREWFERLDRPVLRPLPPRRYEVATFKQCRVNVDYHVEIDGHYYSVPHALVRKAVEARVTRHTIEILYGGKRVALHARNTRKGSHSTVKEH
MPVAHRAHLEWTPGRLLNWAASIGPNVAVIVEYQLTHKSHPEMGYRACLGLLSLAKKYSKERLEAACARAVAIGSLTRKSVVSILENHLDRQATLPVSQT
EWHSPMHDNVRGPDYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa18402595+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLMQHTVGQLKALKLDGMARAFEEQSALTASSSLPFEERFSMLVDREIAWRDTRRLERLLRSAKLKHAQACLEDVVYDGSRGLDQRLVASLASCDWIRNA
QSLILTGATGAGKSWLACAFAQQACRQGFSALYLRVPRLFEELQIAHGDGSFTRRLAQLARIDVLVLDDWGLQEPSKSARGDLLEVLDDRVGTRSTIVTS
QLPIEHWHQWLDDPTLADAILDRLVHQAHKVSLKGESMRKRSATDVKKRAS

 

Blast result :
Comments
ISRme9 is 83%(istA) aa similar to ISBmu3, and 91%(istB) aa similar to IS408.
References
1] Taghavi,S., Provoost,A., Mergeay,M. and van der Lelie,D. (1996) Mol. Gen. Genet. 250 (2), 169-179.