ISDra8

  • Family IS66
  • Group ISBst12
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_000959 ND Deinococcus radiodurans
Deinococcus radiodurans R1 plasmid CP1
DNA section
IS Length : 1452 bp

Ends


IR Length : 19/24

IRL : GTAACTGCTCAGCAGGGGGACTTGCGAAGCGGAAACGGGTAGAGTAGGGG
IRR : GTAACTGCTCAGCACGAGAAGTCGAGTTTGGGTAAGACGCCACGAAAAAC

Insertion site


Left flankDirect repeatRight flankDR Length
TTCTATCGGATATACATTTGAGCCGTAGAGG8

DNA sequence

GTAACTGCTCAGCAGGGGGACTTGCGAAGCGGAAACGGGTAGAGTAGGGGCATCACGCAAGAGACCTGTCCCAACTGCGAACGACTTGCTGCCGAGGTGA
CTCGCCTCCAGGCTGAAGTCGCCGCATTGCAAGCCGAGCTGCGGGCTATTCAAGCCCTTCTTGGTCTGGATAGCCGCAACTCCAATCAACCGCCGAGCAA
AGACCCACCCTGGAAACCCAAAAGCGAGGCCAGAAGAGCGTACGCTCTTCTGGTGGACAGCTAGGACACCCAGGGAAAACACTGAAATTCAGTGACCAGC
CCGACGAAATTCAAACCCTTCCGCTCACGGGAACGTGTCCTTGTGGAACGAGCTGGGAAGAGGTGGAGGTCGCGAACTGCTTTGCCAGGCAGGTTCACGA
CCTGCCAGAAATCCGCCTTCACATCACCGAGTATCAGGCCGAAGTGAAACGCTGTCCGAACTGCGGCTGTCGTGGACAGGCCGCTTTTCCCGAGCATGTC
CCTGGTCAGGTGCAGTACGGCCCGCGACTCCATGCCTTCACGACCTTGCTGAACGTGGGGCATTTCATCCCATTGGAACGGGTCACTCAGCTGACGGACG
CGCTCTTCGGCGCGTCCATCAGCGATGGTACGGTGGCGCTCAACATCAACCTGGCCTCAGAACGCCTTGAGCCTTTCGAGGACGACCTGAAAACTGGCTT
GAGGCAACAAGCTGTACTTCATGCGGATGAAACAGGCGCAAAGGTGAATGGGAAGTTGAACTGGTTTCACGTGGCCTGTTTTGCGAGGGGAACGCTCTAT
ACCTTGCACGAGAAGCGCGGCTACGCGGCTCTTGAAGCGGCTGGCGTGTTGACCGACTTTACGGCGTCGTGGTTCACGACGCCTGGAGTACCTATTTCCG
TCTTCCTGGAGAACATGCACTGTGCAATGCTCACCTGCTGCGCGAACTGCGCAAACTGGATGAGCATGACCGGCAACCCTGGGCCGGTGAACTGCGGCGG
GCGTTGCAGCAGGTCTACCATGCCCAGAAGACCGGGACACTGACGGATGAGCAAAGAGCTGTGTTCTATACGCGATTTGACGAACTGGTGCTGGCTGGCC
TGGAAGCAAATCCAGTACAGGAACCTGTTCCAAAGCGGCGAGGGAAGCCCAAACAGCTTCCGGGGCGCAACCTGGCCTTGCGGTGTCAGCAGCACCGCGC
AGCGATGCTGCTTTTCCTGGAGCGTGCTGACGTGCCGTTTGACAACAATCAGGCGGAACGTGACATCAGGATGGCCTGTGTAAAACGCAAGGTTTCGGGT
GGGTTTCGCTCGGAGGTCGGAGGTCAGGCGTTCTGCCGGGTTCGCAGCTTCATTTCTACCCTTCAGAAGCAGGGCCTATCAGTTTGGACGGGCTTGGTTG
ACGTTTTTCGTGGCGTCTTACCCAAACTCGACTTCTCGTGCTGAGCAGTTAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1344 bp448 aa1031446+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRLQAEVAALQAELRAIQALLGLDSRNSNQPPSKDPPWKPKSEXQKSVRSSGGQLGHPGKTLKFSDQPDEIQTLPLTGTCPCGTSWEEVEVANCFARQVH
DLPEIRLHITEYQAEVKRCPNCGCRGQAAFPEHVPGQVQYGPRLHAFTTLLNVGHFIPLERVTQLTDALFGASISDGTVALNINLASERLEPFEDDLKTG
LRQQAVLHADETGAKVNGKLNWFHVACFARGTLYTLHEKRGYAALEAAGVLTDFTGVVVHDAWSTYFRLPGEHALCNAHLLRELRKLDEHDRQPWAGELR
RALQQVYHAQKTGTLTDEQRAVFYTRFDELVLAGLEANPVQEPVPKRRGKPKQLPGRNLALRCQQHRAAMLLFLERADVPFDNNQAERDIRMACVKRKVS
GGFRSEVGGQAFCRVRSFISTLQKQGLSVWTGLVDVFRGVLPKLDFSC

 

Blast result :
Comments
ISDra8 is 92% aa similar to ISDge4. The transposase was reconstructed in silico by deletion of ISDra1 and of one DR generated by the insertion of ISDra1 into ISDra8.
References
1] White,O., Eisen,J.A., Heidelberg,J.F., Hickey,E.K., Peterson,J.D., Dodson,R.J., Haft,D.H., Gwinn,M.L., Nelson,W.C., Richardson,D.L., Moffat,K.S., Qin,H., Jiang,L., Pamphile,W., Crosby,M., Shen,M., Vamathevan,J.J., Lam,P., McDonald,L., Utterback,T., Zalewski,C., Makarova,K.S., Aravind,L., Daly,M.J., Minton,K.W., Fleischmann,R.D., Ketchum,K.A., Nelson,K.E., Salzberg,S., Smith,H.O., Venter,J.C. and Fraser,C.M.(1999)Science 286 (5444), 1571-1577