ISLxc4
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| EF433175 | ND | Leifsonia xyli | Leifsonia xyli subsp. cynodontis |
DNA section
IS Length : 1311 bp
Ends
IR Length : 28
IRL : GGGTGGGTTCTAACAGTGGTCGCAACACCGGCTAGTTTCGGGAGTTGTTA
IRR : GGGTGGGTTCTAACAGTGGTCGCAACACTCATGGATGGTGTGTTTGTTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCCTTGACA | AGCCGATACA | 0 |
DNA sequence
GGGTGGGTTCTAACAGTGGTCGCAACACCGGCTAGTTTCGGGAGTTGTTATGACACTTGTAAGGACGTCGCAGAAGATGACCGGGCCGGCACCGATGTCG
GCCGAGCGTGCATGATATATCGAGTTGTGGCGGCAGGGAATGAACACCTCCGAGATCTGCCGGATGCTCGGGATTCGCCGCAAGCTGGGCAACAACTGGC
GGAACGGGTGGAAACATCGCGACCTGGTCACGGGCAAAGTGCGTTACTATCCGCCGATCGATGAGGTTCCGGTGGTGACGACGGTCTCGGCCCGGTTCTT
ATCGGAGGACGAGCGGGTGAGGATCGCCGACCAACTCAGGACGGGCGCGAGCATCCGCAGTATCGCAGCCGACCTGGGACGAGCACCGTCGACAATCAGC
CGGGAAGTGCGCCGCAATCGCGGGATCACTGGGGAAGTATCGACCGTTCCATGCGCACCGGCTCGCGAGGAGTCGCCGTGCCCGTGACCGGCCGGGGAAG
ATCGCCTCCAACCCCCGATTGCAGCAGGAGATCCGTGGGCTGTTGAAGAAGTATTGGAGTCCCGTTCAGATCTGTCAGCACCTGCGTCAGCAGCATCCTG
ACGATCCGAGCATGCGCGTGGTGCATGAGACGATTTACCGCGACCTCTACGACTACCGCGGCGGCGCACTCCCGCGCGAACTCTGCCGAATGCTGCGCAC
CAAACGCGACAGACGCAAAGCACCCCGCGTGATCGCCCGGCGCAGGATCCGATTCAACGCTGCGCTCACGATCCATGACCGTCCGTTCGCGCCCACGGAC
CGCAGCGTCCCCGGAGCCTGGGAAGGCGACCTCATCATGGGGCTCGGCAACCGTTCGGCGATTGCCACACTCGTCGAGCGAACGACCCGGTTCACTCTCC
TGTTGCCTGTTGACGCTGTCAACAGATCCGAGAGCCTCCGCGATCAGCTCGTCCCTGCTCTCGCGGCGCTCCCGCCCGAACTACGCCGCTCAATCACCTG
GGACCAGGGCTGGGAAATGGCGAGACATGAAGAGATCAGCCGCGCAACAGGAACGAGAATCTACTCCTGCGACCCGCACTCGCCCTGGCAGCGCGGCAGC
AACGAGAACACGAATCGGCTCTTGCGGGACTACTTCCCCAAACGCACCGACCTCAGAACACACACCCCGAAAGAGCTCGCCCTCGTCGCCGCCGAACTCA
ACAACCGACCCCGCAAAATCCTCGGCTGGAAGACCCCGAACACCCTCTTCACTACGCTGCTAGAACAAACACACCATCCATGAGTGTTGCGACCACTGTT
AGAACCCACCC
GCCGAGCGTGCATGATATATCGAGTTGTGGCGGCAGGGAATGAACACCTCCGAGATCTGCCGGATGCTCGGGATTCGCCGCAAGCTGGGCAACAACTGGC
GGAACGGGTGGAAACATCGCGACCTGGTCACGGGCAAAGTGCGTTACTATCCGCCGATCGATGAGGTTCCGGTGGTGACGACGGTCTCGGCCCGGTTCTT
ATCGGAGGACGAGCGGGTGAGGATCGCCGACCAACTCAGGACGGGCGCGAGCATCCGCAGTATCGCAGCCGACCTGGGACGAGCACCGTCGACAATCAGC
CGGGAAGTGCGCCGCAATCGCGGGATCACTGGGGAAGTATCGACCGTTCCATGCGCACCGGCTCGCGAGGAGTCGCCGTGCCCGTGACCGGCCGGGGAAG
ATCGCCTCCAACCCCCGATTGCAGCAGGAGATCCGTGGGCTGTTGAAGAAGTATTGGAGTCCCGTTCAGATCTGTCAGCACCTGCGTCAGCAGCATCCTG
ACGATCCGAGCATGCGCGTGGTGCATGAGACGATTTACCGCGACCTCTACGACTACCGCGGCGGCGCACTCCCGCGCGAACTCTGCCGAATGCTGCGCAC
CAAACGCGACAGACGCAAAGCACCCCGCGTGATCGCCCGGCGCAGGATCCGATTCAACGCTGCGCTCACGATCCATGACCGTCCGTTCGCGCCCACGGAC
CGCAGCGTCCCCGGAGCCTGGGAAGGCGACCTCATCATGGGGCTCGGCAACCGTTCGGCGATTGCCACACTCGTCGAGCGAACGACCCGGTTCACTCTCC
TGTTGCCTGTTGACGCTGTCAACAGATCCGAGAGCCTCCGCGATCAGCTCGTCCCTGCTCTCGCGGCGCTCCCGCCCGAACTACGCCGCTCAATCACCTG
GGACCAGGGCTGGGAAATGGCGAGACATGAAGAGATCAGCCGCGCAACAGGAACGAGAATCTACTCCTGCGACCCGCACTCGCCCTGGCAGCGCGGCAGC
AACGAGAACACGAATCGGCTCTTGCGGGACTACTTCCCCAAACGCACCGACCTCAGAACACACACCCCGAAAGAGCTCGCCCTCGTCGCCGCCGAACTCA
ACAACCGACCCCGCAAAATCCTCGGCTGGAAGACCCCGAACACCCTCTTCACTACGCTGCTAGAACAAACACACCATCCATGAGTGTTGCGACCACTGTT
AGAACCCACCC
Recoding section
- Recoding by frameshift
- Frame +1
- Type unknow
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence : No
- Secondary structure : No-structure
Recoding motif :
GCGGGATCACT GGGGAAG TATCGACCGTTCC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 348 bp | 115 aa | 140 | 487 | + | No |
Description : First part of the transposase
ORF sequence :
MNTSEICRMLGIRRKLGNNWRNGWKHRDLVTGKVRYYPPIDEVPVVTTVSARFLSEDERVRIADQLRTGASIRSIAADLGRAPSTISREVRRNRGITGEV
STVPCAPAREESPCP
STVPCAPAREESPCP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 963 bp | 320 aa | 321 | 1283 | + | No |
Description : Second part of the transposase
ORF sequence :
GSPTNSGRARASAVSQPTWDEHRRQSAGKCAAIAGSLGKYRPFHAHRLARSRRARDRPGKIASNPRLQQEIRGLLKKYWSPVQICQHLRQQHPDDPSMRV
VHETIYRDLYDYRGGALPRELCRMLRTKRDRRKAPRVIARRRIRFNAALTIHDRPFAPTDRSVPGAWEGDLIMGLGNRSAIATLVERTTRFTLLLPVDAV
NRSESLRDQLVPALAALPPELRRSITWDQGWEMARHEEISRATGTRIYSCDPHSPWQRGSNENTNRLLRDYFPKRTDLRTHTPKELALVAAELNNRPRKI
LGWKTPNTLFTTLLEQTHHP
VHETIYRDLYDYRGGALPRELCRMLRTKRDRRKAPRVIARRRIRFNAALTIHDRPFAPTDRSVPGAWEGDLIMGLGNRSAIATLVERTTRFTLLLPVDAV
NRSESLRDQLVPALAALPPELRRSITWDQGWEMARHEEISRATGTRIYSCDPHSPWQRGSNENTNRLLRDYFPKRTDLRTHTPKELALVAAELNNRPRKI
LGWKTPNTLFTTLLEQTHHP
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1144 bp | 381 aa | 140 | 1283 | + | Yes |
Chemistry : DDE
ORF sequence :
MNTSEICRMLGIRRKLGNNWRNGWKHRDLVTGKVRYYPPIDEVPVVTTVSARFLSEDERVRIADQLRTGASIRSIAADLGRAPSTISREVRRNRGITGGK
YRPFHAHRLARSRRARDRPGKIASNPRLQQEIRGLLKKYWSPVQICQHLRQQHPDDPSMRVVHETIYRDLYDYRGGALPRELCRMLRTKRDRRKAPRVIA
RRRIRFNAALTIHDRPFAPTDRSVPGAWEGDLIMGLGNRSAIATLVERTTRFTLLLPVDAVNRSESLRDQLVPALAALPPELRRSITWDQGWEMARHEEI
SRATGTRIYSCDPHSPWQRGSNENTNRLLRDYFPKRTDLRTHTPKELALVAAELNNRPRKILGWKTPNTLFTTLLEQTHHP
YRPFHAHRLARSRRARDRPGKIASNPRLQQEIRGLLKKYWSPVQICQHLRQQHPDDPSMRVVHETIYRDLYDYRGGALPRELCRMLRTKRDRRKAPRVIA
RRRIRFNAALTIHDRPFAPTDRSVPGAWEGDLIMGLGNRSAIATLVERTTRFTLLLPVDAVNRSESLRDQLVPALAALPPELRRSITWDQGWEMARHEEI
SRATGTRIYSCDPHSPWQRGSNENTNRLLRDYFPKRTDLRTHTPKELALVAAELNNRPRKILGWKTPNTLFTTLLEQTHHP
Blast result :
Comments
No DR was identified in any of the five copies of ISLxc4 sequenced by our group.
There is a purine rich site (ntd 431-437) within ISLxc4 that may promote the slippery of codon during translation. If it is true, the coupled translation of both ORFs would result in a 381 amino acid long transposase (ORF3).
ISLxc4 is respectively 67% aa similar to ISLxc3.
There is a purine rich site (ntd 431-437) within ISLxc4 that may promote the slippery of codon during translation. If it is true, the coupled translation of both ORFs would result in a 381 amino acid long transposase (ORF3).
ISLxc4 is respectively 67% aa similar to ISLxc3.
References
1] Zerillo,M.M. (2008) Direct submission.