ISAzo9

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Azoarcus sp. EbN1
DNA section
IS Length : 1483 bp

Ends


IR Length : 26/30

IRL : GTACCTCTGCACAGAGGATTTGACGGGTTGATGGTCGGGTGAGCGCAGGA
IRR : GTACCTCTGCACAGAGGTTATGACTGTTTGGCTGGGTGCGGGTAAGCGCG

Insertion site


Left flankDirect repeatRight flankDR Length
GTGAAGAAGCTAGCGGTATCAC2

DNA sequence

GTACCTCTGCACAGAGGATTTGACGGGTTGATGGTCGGGTGAGCGCAGGAGGGGCAGGGCTTCGGGCGGGGCGAGGAGTCGGATGCTGCGCGCGACTCCA
GGTTGTCGGCTGAGGAGTCCCAGGCGCTCGAGCGTGAGCACCATCTGATGCACCGAGGGGGGCGTGACGTGGAAGTAGCGTTGCAGGTCGGCTTCGGCCG
GCGGCCGGCCGAGCACTTGGGTGTAAGCGTAGATGAACGCGAGGTACTGCCCTTGCACCGGTGTGAAGCGCGGTGACGAAGGGGTGAGTTCCGCGTCGGA
GTTCTGGCTCATTTGTTGTCTCTCCAGATTTTGGAGGGGCCGCGATGAATGTACGCTACCGGGTCGAATTGAGCCAAGCCGAACGCGACGAATTGAGGAT
GCTGCTGGGCGGCGGCAAGGCAGCGGTGCGCAAGGTGAAACGCGCGCAGATCCTGCTGGCGGCCGATGCCGGAGCCGGCGACGAGGAGATTGCCACGAAC
GTCGGAGTGGGCAGCTCGACGGTCTATCGGACCAAGCGCCGCTTCGTGCTGGGCAACGTCGAGGCGGCGCTCAGCGACGAGCCGCGCCCCGGGGCGGAGC
GCAAGCTCACCGGCAAGGAAGAGGCGCTGCTGGTCGCCACCGCCTGCACCAAGCCGCCAACGGGGCGGTCGCGCTGGACGCTCGAATTGCTGGCGGGGGA
GATAGTCAAGCTCACCGAGCACGCGAGCCTGTCACGCGAGACGGTGCGCCGCCGCCTGGCGGAGAACGAGCTCAAGCCTTGGCGCCAGAAGATGTGGTGC
ATCCCGCAGGTCGACGGTCACTACGTTGCCCGGATGGAGGACGTGCTCGACCTCTACGCCGAAGCCCCCGATCCCGAGCGCCCGGTGGTCTGCTTCGATG
AAAGTCCCACCCAGCTCATCGGCGAGGTGCGCCAGCCCATCCCGGCGATGCCCGGACAGCTCGAGCGCTACGACTACGAGTATCGCCGCAACGGGACCGC
CAACCTGTTCGTCTTCCTCGACGCGCACCGGTCCTGGCGCCGGGTCAAGGTAACCGAGCAGCGCACTGCGCTCGACTTCGCCGAATGCATGCGCGAACTG
GTCGACTGCCACTATCCGCAGGCCGAGCGTATCCGTGTTGTCCTCGACAACCTGTCGACCCATACGCCCGGCGCACTCTATGAAACCTTCCCGGCGCCCG
AAGCGCATCGGTTGCTGCATCGTCTGGAATTCCATTACACCCCCAAGCACGCCAGCTGGCTCAACATGGTCGAGATCGAAATCGGGGTGCTGCGCAGCCA
GTGCCTCGACCGGCGCATCGGCGACCGCGACCGACTCGTCAGCGAAATTGCAGCCTGGGAGCAGCAGCGCAACGCCATCGGCGCTCGCATCCACTGGATG
TTCACCACCGACCGCGCCCGGAGCAAACTGGCCCGCGCTTACCCGCACCCAGCCAAACAGTCATAACCTCTGTGCAGAGGTAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
312 bp104 aa1312-No
ORF function : Passenger Gene
Annotation : Helix-turn-helix diphteria tox regulatory ElementDescription :

ORF sequence :

MSQNSDAELTPSSPRFTPVQGQYLAFIYAYTQVLGRPPAEADLQRYFHVTPPSVHQMVLTLERLGLLSRQPGVARSIRLLAPPEALPLLRSPDHQPVKSS
VQRY

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1122 bp373 aa3451466+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNVRYRVELSQAERDELRMLLGGGKAAVRKVKRAQILLAADAGAGDEEIATNVGVGSSTVYRTKRRFVLGNVEAALSDEPRPGAERKLTGKEEALLVATA
CTKPPTGRSRWTLELLAGEIVKLTEHASLSRETVRRRLAENELKPWRQKMWCIPQVDGHYVARMEDVLDLYAEAPDPERPVVCFDESPTQLIGEVRQPIP
AMPGQLERYDYEYRRNGTANLFVFLDAHRSWRRVKVTEQRTALDFAECMRELVDCHYPQAERIRVVLDNLSTHTPGALYETFPAPEAHRLLHRLEFHYTP
KHASWLNMVEIEIGVLRSQCLDRRIGDRDRLVSEIAAWEQQRNAIGARIHWMFTTDRARSKLARAYPHPAKQS

 

Blast result :
Comments
The first ORF is a passenger gene, the second is the transposase. ISAzo9 is 63% aa similar to ISMac17.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).