ISPre4

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_004444 ND Pseudomonas resinovorans
Pseudomonas resinovorans plasmid pCAR1
DNA section
IS Length : 2575 bp

Ends


IR Length : 44/49

IRL : TGCGGATTCCACGGCCATCCGGCCACCTATTCCATAAACATCCGGCCACT
IRR : TGCGCATTCCACGCCATCCGGCCACCCAGTCCACCAACATCCGGCCACCC

Insertion site


Left flankDirect repeatRight flankDR Length
ATACCCAAGATGCAGAAAGCC7

DNA sequence

TGCGGATTCCACGGCCATCCGGCCACCTATTCCATAAACATCCGGCCACTGATTCCACGATGATCCGGCCACCCATTCCACGCTCATCCGGCCACCCTTA
AGGCAGGCAGCAACGCAGGATTATTCACTACCATCGACCTCTTTTATCGAAGCAGAGAGGTCGTCGTGGAGCGTTTATCCATGCGTAAAATTCGAGAAGT
GCTACGTCTCAAATTCGAGGTCGGCCTATCTGCCCGCCAGGTCGCTGGCAGCTTGCAAGTAGGCCGAGCCAGCGTCGGTGAGTACCTCAATCGCTTTGCT
GCCAGCGGCCTGACCTGGCCCTCTGCGCTGACTGACGCCGAGTTACAACGGCATCTTTTTCCGCCGCCGCCCACCGTTCCCAGCGATCAAAGGCCGGTGC
CAGACTGGGCTCATGTACACGGGGAGTTACGCCGCCCCGGCGTAACTCTGGCCCTGCTTTGGCAGGAATACCGACTCGCCCACCCGCAAGGCTTCCAATA
CAGCTGGTTCTGCGAGCACTACCGCCTCTGGGCCGCCAAGGTCGACGTGGTCATGCGCCAGGAGCACCGTGCCGGCGAAAAGCTGTTCGTCGATTACGCC
GGCCAGACCGTACCGGTCATTGATCGGCGAACCGGCGAGATCCGCCAAACGCAGATCTTCGTCGCCGTTCTCGGCGCGTCCAGCTACACCTTTGCCGAGG
CCACCTGGTCGCAGAAGCTGCCTGACTGGCTGGGCTCGCACGCCCGCTGTTTTGCTTTTTTCGGTGGCACATCGCAGATCCTGGTGCCCGATAATCTGCG
CAGCGGCGTCACCAAGGCGCATCGCTACGAGCCCGACATCAACCCCAGTTACCGCGATCTCGCCGAGCATTACGGCATCGCCGTATTACCTGCTCGCTCG
CGCAAACCCAAGGACAAAGCCAAGGTCGAAGTCGGCGTGCAAGTCGTCGAGCGCTGGATCCTCGCCGCGCTGCGCAACCGCCAGTTCTTCTCGCTGGGCG
AACTCAACACGGCAATCAGCCTGCTGCTCGAACGCCTCAATCAAAAGCCCTTCAAGAAGCTGCCCGGCTCACGCCGCTCGGCCTTTGAGACCATCGACCA
ACCCGCACTGCAACCGCTACCAGAACATCCGTACATCTACGCCGAATGGAAAAAGGTGCGGGTGCACATCGACTACCACGTCGAGGTCGAGGGCCATTAC
TACTCGGTGCCGTACCAGTTGGTGAAGCACCAACTCGAAGTGCGGCTAACCGCTAAGACCGTCGAGTGCTTCCACGCCAACCAGCGGGTGGCCAGTCACC
TGCGCTCGCTGCACAAAGGCCGCCACACCACCCAAACCGAGCACATGCCCAAGAGCCACCGGGAGCACGCCGAATGGACACCGCAACGGCTGATCCGCTG
GGCCGAGCAGACCGGGCCTAACACCGCTGGCGTTATCGCCTACATCCTTGAGCGCCGAATCCATCCTCAGCACGGCTTCCGCGCCTGCCTGGGCATCCTG
CGCCTGAGCAAGCAGCACGGCGAAGAGCGGCTGGAAGCCGCTTGCCAGCGAGCGCTGGCACTGGGCGCGTGCAGCTACAAAAGCCTTGAATCGATCCTGC
GCCAAGGGCTGGAAAAGCTACCGCTGTCCCAGCAAAACCTGCCGCTACTGCCCGACGAACACATCAACCTGCGCGGCCCTGGCTACTACCACTGACCTGA
AAAAGGAGCACCAAAATGCTGCCCAATCCGACACTCGACAAGCTGCAAACCCTGCGCCTGCACGGCATGATCAAGGCGCTGAGCGAGCAACACGCCACGC
CCGATATCAATGATCTGAGCTTCGACGAACGCCTCGGCCTGATGATCGACCGCGAGCTGACCGAACGTGAAAACACGCGTCTGAGCAGCCGGCTGAAACT
GGCACGACTGCGCCACAACGCTTGCCTGGAAGACATCGATTACCGCAGCCCACGCGGCCTGGACAAGTCCCTGATCCTGCAACTGGGCAGCGGCCAATGG
CTGCGCGATGGCTTGAACCTGATCATCGGCGGCCCGACCGGTGTAGGCAAAACCTGGCTCGCCTGCGCGCTGGCCCACAAGGCCTGCCGTGACGGCTACA
GCGTGCGCTACTTGCGTCTGCCGCGCCTGATGGAGGAACTGGGCCTGGCCCACGGAGACGGTCGATTCGCGAAACTGATGGCGGGCTATGCCAAGACCGA
CTTGCTGATCCTGGACGACTGGGGCCTGGCGCCGTTTACCGCACCGCAACGGCGCGACATGCTTGAGCTATTGGACGACCGCTACGGCAACCGCTCGACG
CTGGTCACCAGCCAGATGCCCGTGGACAAATGGCATGCACTGATCGGCGATCCGACCTTGGGCGACGCGATCCTCGACCGGCTGGTGCACAACGCTTATC
GGATCGAACTGAAGGGCGAATCGATGCGCAGACGCGCAACGAAATTGACGACGACAGGGACTTCAGACTAACAATGCAAACCTGCGTCGCTGCGCTCCGA
CTGCCTGGCCGGATGAGCGTGGAACGGGTGGCCGGATGTTGGTGGACTGGGTGGCCGGATGGCGTGGAATGCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1530 bp509 aa1661695+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MERLSMRKIREVLRLKFEVGLSARQVAGSLQVGRASVGEYLNRFAASGLTWPSALTDAELQRHLFPPPPTVPSDQRPVPDWAHVHGELRRPGVTLALLWQ
EYRLAHPQGFQYSWFCEHYRLWAAKVDVVMRQEHRAGEKLFVDYAGQTVPVIDRRTGEIRQTQIFVAVLGASSYTFAEATWSQKLPDWLGSHARCFAFFG
GTSQILVPDNLRSGVTKAHRYEPDINPSYRDLAEHYGIAVLPARSRKPKDKAKVEVGVQVVERWILAALRNRQFFSLGELNTAISLLLERLNQKPFKKLP
GSRRSAFETIDQPALQPLPEHPYIYAEWKKVRVHIDYHVEVEGHYYSVPYQLVKHQLEVRLTAKTVECFHANQRVASHLRSLHKGRHTTQTEHMPKSHRE
HAEWTPQRLIRWAEQTGPNTAGVIAYILERRIHPQHGFRACLGILRLSKQHGEERLEAACQRALALGACSYKSLESILRQGLEKLPLSQQNLPLLPDEHI
NLRGPGYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa17162471+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLPNPTLDKLQTLRLHGMIKALSEQHATPDINDLSFDERLGLMIDRELTERENTRLSSRLKLARLRHNACLEDIDYRSPRGLDKSLILQLGSGQWLRDGL
NLIIGGPTGVGKTWLACALAHKACRDGYSVRYLRLPRLMEELGLAHGDGRFAKLMAGYAKTDLLILDDWGLAPFTAPQRRDMLELLDDRYGNRSTLVTSQ
MPVDKWHALIGDPTLGDAILDRLVHNAYRIELKGESMRRRATKLTTTGTSD

 

Blast result :
Comments
ISPre4 is 94%(ORF1) and 97% (ORF2) aa similar to ISPsy14.
References
1] Urata,M., Miyakoshi,M., Kai,S., Maeda,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H. (2004) J. Bacteriol. 186 (20), 6815-6823
2] Maeda,K., Nojiri,H., Shintani,M., Yoshida,T., Habe,H. and Omori,T.
(2003) J. Mol. Biol. 326 (1), 21-33