ISPre1
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004444 | ND | Pseudomonas resinovorans | Pseudomonas sp. LM12 Pseudomonas resinovorans plasmid pCAR1 |
DNA section
IS Length : 1192 bp
Ends
IR Length : 12/16
IRL : GGAAACTCTGAAGAAGACTTCCAGATTTTGGCAAAATACCCGGATTCCAC
IRR : GGATGGTCTGAAGTAGTTGTGTATTTCTGGCTGACTTCAGCCCCTGCCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCAGACTCT | CTAG | CCTGTTAATT | 4 |
| ACTGCACAAG | TTAA | CGTATCAGCA | 4 |
DNA sequence
GGAAACTCTGAAGAAGACTTCCAGATTTTGGCAAAATACCCGGATTCCACCCGCTGAGTTTCCCCATGAAGCAGATGACCTTCGCTGACGCCGAGTACGC
CGGTAAGCGCAAACAAACCCGCAAAGAGTTGTTCCTGATCGAGATGGATCGGGTGGTGCCGTGGAAGGGTTTGATTGCCTTGATCGAACCGCATTATCCC
AAGGGTGAAGGCGGTCGTCCGGCCTATCCGCTGATGGCGATGCTGCGCGTGCACCTGATGCAAAACTGGTTCGGTTACAGCGATCCAGCGATGGAAGAGG
CGCTGTACGAGACCACCATCCTGCGCCAGTTCGCGGGGCTGAGCTTGGAGCGTATCCCCGATGAAACCACCATCCTCAACTTCCGTCGTTTGCTGGAGAA
GCACGAACTAGCCGCCGGCATCTTGGCTGTCATCAATGGCTATCTGGGCGACCGAGGGCTGTCGCTGCGTCAAGGCACGATCGTCGATGCCACGCTGATC
AATGCGCCGAGTTCGACCAAGAACAAGGACGGTAAGCGCGACCCGGAAATGCATCAGACCAAGAAGGGTAACCAGTACTACTTCGGCATGAAGGCGCACA
TCGGTGTCGATGATGAGTCGGGCTTGGTGCACAGCGTGGTGGGCACGGCGGCCAACGTGGCCGATGTCACCCAGGTCGATAAGCTGCTGCACGGCGATGA
GAACATGGTCGGTGCCGACGCGGGTTACACCGGCGTCGAGAAACGCCCTGAACATGAAGGCCGGGAGGTCATCTGGCAGATCGCCGCCCGCCGTAGTACT
TACAAGAAGCTTGATAAGCGCAGTGCTTTGTACAAAGCCGCGCGCAAGATTGAGAAGGCCAAGGCTCAGGTACGAGCCAAGGTCGAGCATCCGTTCCGGG
TGATCAAGCGCCAGTTCGGTTATGTGAAGGTGCGCTTCCGTGGTCTGGCGAAGAACACGGCGCAACTGGTGACGCTGTTCGCGCTGTCGAACCTGTGGAT
GGCGCGCCGACATTTACTGGCGAATGCAGGAGAGGTGCGCCTGTAATGTGGGAAATGGCTGCCGCGAGGTGCTCGCGGCGGCTAAAAACACAGAAATAAG
CGGGTGATCTGAGCGTTTTTGATCGATTTGCCGCTTTTAAAATCGGCAGGGGCTGAAGTCAGCCAGAAATACACAACTACTTCAGACCATCC
CGGTAAGCGCAAACAAACCCGCAAAGAGTTGTTCCTGATCGAGATGGATCGGGTGGTGCCGTGGAAGGGTTTGATTGCCTTGATCGAACCGCATTATCCC
AAGGGTGAAGGCGGTCGTCCGGCCTATCCGCTGATGGCGATGCTGCGCGTGCACCTGATGCAAAACTGGTTCGGTTACAGCGATCCAGCGATGGAAGAGG
CGCTGTACGAGACCACCATCCTGCGCCAGTTCGCGGGGCTGAGCTTGGAGCGTATCCCCGATGAAACCACCATCCTCAACTTCCGTCGTTTGCTGGAGAA
GCACGAACTAGCCGCCGGCATCTTGGCTGTCATCAATGGCTATCTGGGCGACCGAGGGCTGTCGCTGCGTCAAGGCACGATCGTCGATGCCACGCTGATC
AATGCGCCGAGTTCGACCAAGAACAAGGACGGTAAGCGCGACCCGGAAATGCATCAGACCAAGAAGGGTAACCAGTACTACTTCGGCATGAAGGCGCACA
TCGGTGTCGATGATGAGTCGGGCTTGGTGCACAGCGTGGTGGGCACGGCGGCCAACGTGGCCGATGTCACCCAGGTCGATAAGCTGCTGCACGGCGATGA
GAACATGGTCGGTGCCGACGCGGGTTACACCGGCGTCGAGAAACGCCCTGAACATGAAGGCCGGGAGGTCATCTGGCAGATCGCCGCCCGCCGTAGTACT
TACAAGAAGCTTGATAAGCGCAGTGCTTTGTACAAAGCCGCGCGCAAGATTGAGAAGGCCAAGGCTCAGGTACGAGCCAAGGTCGAGCATCCGTTCCGGG
TGATCAAGCGCCAGTTCGGTTATGTGAAGGTGCGCTTCCGTGGTCTGGCGAAGAACACGGCGCAACTGGTGACGCTGTTCGCGCTGTCGAACCTGTGGAT
GGCGCGCCGACATTTACTGGCGAATGCAGGAGAGGTGCGCCTGTAATGTGGGAAATGGCTGCCGCGAGGTGCTCGCGGCGGCTAAAAACACAGAAATAAG
CGGGTGATCTGAGCGTTTTTGATCGATTTGCCGCTTTTAAAATCGGCAGGGGCTGAAGTCAGCCAGAAATACACAACTACTTCAGACCATCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 981 bp | 326 aa | 66 | 1046 | + | No |
Chemistry : DDE
ORF sequence :
MKQMTFADAEYAGKRKQTRKELFLIEMDRVVPWKGLIALIEPHYPKGEGGRPAYPLMAMLRVHLMQNWFGYSDPAMEEALYETTILRQFAGLSLERIPDE
TTILNFRRLLEKHELAAGILAVINGYLGDRGLSLRQGTIVDATLINAPSSTKNKDGKRDPEMHQTKKGNQYYFGMKAHIGVDDESGLVHSVVGTAANVAD
VTQVDKLLHGDENMVGADAGYTGVEKRPEHEGREVIWQIAARRSTYKKLDKRSALYKAARKIEKAKAQVRAKVEHPFRVIKRQFGYVKVRFRGLAKNTAQ
LVTLFALSNLWMARRHLLANAGEVRL
TTILNFRRLLEKHELAAGILAVINGYLGDRGLSLRQGTIVDATLINAPSSTKNKDGKRDPEMHQTKKGNQYYFGMKAHIGVDDESGLVHSVVGTAANVAD
VTQVDKLLHGDENMVGADAGYTGVEKRPEHEGREVIWQIAARRSTYKKLDKRSALYKAARKIEKAKAQVRAKVEHPFRVIKRQFGYVKVRFRGLAKNTAQ
LVTLFALSNLWMARRHLLANAGEVRL
Blast result :
Comments
ISPre1 is 97% aa similar to IS1384.
An isoform of ISPre1 has been identified in Pseudomonas sp. LM12 (KJ920390) by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis, this isoform is present in 2 copies in the genome of Pseudomonas sp. LM12. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM5, Pseudomonas sp. LM6, Pseudomonas mendocina strain LM7, Pseudomonas sp. LM8, Pseudomonas aeruginosa strain LM10, Pseudomonas sp. LM11, Pseudomonas sp. LM13, Pseudomonas sp. LM14, Pseudomonas sp. LM25, Pseudomonas sp. ZM1, Pseudomonas sp. ZM2, Acinetobacter sp. LM3, Brevundimonas sp. LM17.
File updated the January 22 2015 with the information provided by Szuplewska M.
An isoform of ISPre1 has been identified in Pseudomonas sp. LM12 (KJ920390) by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis, this isoform is present in 2 copies in the genome of Pseudomonas sp. LM12. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM5, Pseudomonas sp. LM6, Pseudomonas mendocina strain LM7, Pseudomonas sp. LM8, Pseudomonas aeruginosa strain LM10, Pseudomonas sp. LM11, Pseudomonas sp. LM13, Pseudomonas sp. LM14, Pseudomonas sp. LM25, Pseudomonas sp. ZM1, Pseudomonas sp. ZM2, Acinetobacter sp. LM3, Brevundimonas sp. LM17.
File updated the January 22 2015 with the information provided by Szuplewska M.
References
1] Urata,M., Miyakoshi,M., Kai,S., Maeda,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H. (2004) J. Bacteriol. 186 (20), 6815-6823
2] Maeda,K., Nojiri,H., Shintani,M., Yoshida,T., Habe,H. and Omori,T.
(2003) J. Mol. Biol. 326 (1), 21-33
3] Szuplewska, M., and Bartosik, D. (2014) Direct submission.
2] Maeda,K., Nojiri,H., Shintani,M., Yoshida,T., Habe,H. and Omori,T.
(2003) J. Mol. Biol. 326 (1), 21-33
3] Szuplewska, M., and Bartosik, D. (2014) Direct submission.