ISfinder Mirror Site

ISChy3

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007503	ND	Carboxydothermus hydrogenoformans	Carboxydothermus hydrogenoformans Z-2901

DNA section

IS Length : 3098 bp

Ends

IR Length : 30/48

IRL : TGTTTCTACTCACATAAAATAACCGTCCAATGCTCAGATTTCGTGGCCAA
IRR : TGTTACCGCTCACACTAATTGACCACATTTAGCTCAAATTAAGTGGCCGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CAGAAATGTACCCAC	TTCAGG	TTGTAGGCTCGGGTG	6
AATTTCATACCGTTG	CTTTTC	AAAAATCTTTGCAAT	6

DNA sequence

TGTTTCTACTCACATAAAATAACCGTCCAATGCTCAGATTTCGTGGCCAAATAGAAAAGGAATTTGTTTAGATAATGGCGAATAATTGGAGAAAAAATCT
AAAGAAGGAGAAAAAGGAAAAGTCTTCGAGTAAGGTTTACGGAGCTGACACTCCCCTTACCGAAGACTTTCCTCCATACCGTACTTTGATTGTAACTTAT
CTTGGAGCTTCAGTAAAGGAGTATTTAGAAAATTACTTAAATTTTTTGGAAGAAAACCAATGGTATTGCCCGGTTTGTAGTGCAAAGATGTCTTTTCACG
GATGGTATCGACGAAAAATAATAACCTTAGATGGAACTACTACTAGAATTCCAATAGCCCGCTATCGCTGTTCTAACTGCCGGAAAACCCATGCCATTTT
ACCGGATTTTGTTGCGCCTTACCGCCATTATTCCCAGGTTTTGATTGCAGCTGTAGTTGAAGAAGTAGTTTCCAAGCAGGTACCGCCAGAACGGGTCGAG
GGCAATCAGGATATTCCCACTACTCGGCGATGGATACGCCGATTTCTAAAGCGATGTCATGAGGTTATAGGCGTTTTAGAAAGTATAGCTTTTAGATACA
CCGGGCAAATAAAATCGCTTCTAACGGAAGCACGGTCGTCGCCCTGGGAGCAAATGCTTGCGGCTTTAGAACTACTGCCGGCGGTAAAAGCTACTTCGGT
GTTTGGTGCGGTAAATCTCTGGCTGACCATGGACGTGGTCGGCCTCTGGCTTTAACTAATTTCACATAGTTTGGTCTCCTGGCCATTCTTGGGATTGTGG
TATGGTGAATTTAACACCATATTTAAACAAGGAGATGGTCAGGTTGCCAAAGGAATTATCTTATCAGCAAGAAATTGCTCTTAAACGTTTTGCTCTGATA
GCACCTTTGCTGGAGCCGGATTTAGAAGCGGCGGAAAAGCGCCAACGCCGGAAAGAAATCTTAGCCAGGAGTGAAATTTCCTCCCGTACCCTGCGCCGCT
ACTTACAACTTTACCGCCAGCAGGGTTTATCCGGTCTTATGCCAAAAATCCGGTCTGATAATGGAAGCAGTCGTACTATAAGCCATGAAATAATCGAAGA
GGCGGTAAAACTTAAGGAAGAACTTCCGGAACGGAGTGTAAGCCAAATCATAGCGATTTTAGAAGGAGAAAAGAAAGTTCCTGCAGGAATGTTAGCCCGG
TCTACCCTTGGAAGGCATCTTTCTCGTTTAGGCCTAACCCAAAAGGAAGCTAACCAAAAGATATCAGGGCACAGGCGTTTTGCAAAAGAACAGCGTAATC
GCCTCTGGCAAGCAGATATCAAATATGGGCCGTATCTACCGCATCCAAAGAATCCCAAACGCAAAGTGCGAACTTATTTAGTGGCATTTATCGATGATGC
TACTCGTCTTCTTTGTCATGGAGAGTTTTATCTTGACCAGAAGAGGCCAGTTTTAGAAGATTGTTTTCGTAAAGCCATCTTGAAACGAGGAATCCCGGAT
GCGGTATATGTAGATAATGGCAAGATTTTTGTTTCCCGCTGGTTTCGTTTGGGATGTGCCAGGCTGGGGATAAGGCCAATTAATACTAAGCCATATTCAC
CTGAATCTAAGGGGAAAATTGAAAGGTTTAACCGCACCGTTGAGTCTTTTATAGCCGAAATAGAACTGCAACAACCTGAGACCCTGGCAGAACTTAACCA
GGCTTTTGCTGTTTGGGTTGAAGAAGGTTATAACCACCACCCGCACAGCTCTTTAGAAAACGAAACTCCGGCTAACCGTTTCCAGAAGGATACCCGGCGT
TTACGGTTTGCCAGCCTGGAAGAATGTCGGGAAGCTTTCCTCTGGGAAGCAAGTCGTCGGGTAGATAAAACAGGATGTATCAAACTTGAAGGACGGTTTT
ATGAAATTGGTTTGGAATGGATACGAAAAACCGTTGATCTCCGTTACGACCCTTTTGACTTAGAAAGTATCGAGTTCTGGTATAACGGCCAAAAGCAGGG
ACTGGCCAAGCCTTTGGTTATCCAGGAATACAACAACCTTGCTGCAAAAAATAAAAAAGAGCAAGAAAATAAAACACCAAAAACTTCCCGCCTCTTAACA
GTACTTGAAGAAAGAAGTATTGAACGCCGCCGGCGAAAATTAGGGGCCATACCCTTCCGCCAGCTGGAAGGGGGTAAGTAAAAATGTTTGAACACTTTTT
TGGTTTTCACCGCACCCCCTTCGGGAGGGATCTGCCGGTAAACTCTTTGTTTACCACTCCTGCCCATGAAGAGCTGGTATCCAGGCTTAAATATGCAGCG
GAAAAACGCCTCTTTGCCCTAATTACCGGCGAAGTAGGAGCAGGTAAGTCCACAGCATTACGGCAACTATTATCGCTATTAAACCCCAATAAGTACCGGG
TTATTTATATTAGCGATTCTGGTCTAACTCCCAGGCATTTTTACTGGGAAGCCCTGCGGCAGCTTGACTGTGAGCCACGCTTTAACCGGGGACAGGCTAA
ACGTCAGCTGCATCAGGTTATCCTTGACTTAGCAGAAAATCAACAGAAAATACCGGTAGTTATTGTGGATGAAGCTCATCTCCTTGACCGGGAAATGCTT
GAAGAAATACGCTTTCTTTTGAATTTTCGCATGGATTCGTATAATCCAATGAGTCTAATTCTGGTAGGTCAGCCAGAACTGCGCCGTATCTTACAACTTC
AGGTTTATGAAGCTATTGCTCAGAGGATTAACCTTCGCTACCACCTTCCGCCCATGGAACGGGAGGAAGCAAAAGGCTATGTGGCCCACCATCTCAGGAC
AGCCGGGGCAAGTTCAGCAATTTTTACCGATGATGCCTTGGATATGGTCTATGAGTATGCTGGGGGTATTGCCCGGAAAATCAATAACCTGTGCATTGCT
TGTCTAATGGCAGCTGCAGCCGAACAGAAACGGCTTATTGATGACCGTATGGTCAAGGTAGTAATTGAAAATGAATTTTCTGTATAAAATTATGTCAATG
GCCAGGCAGTATAAAAAACTGCCTGGCCAAATAAAGTGAGCAATATTTGCGGCCACTTAATTTGAGCTAAATGTGGTCAATTAGTGTGAGCGGTAACA

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
681 bp	226 aa	75	755	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MANNWRKNLKKEKKEKSSSKVYGADTPLTEDFPPYRTLIVTYLGASVKEYLENYLNFLEENQWYCPVCSAKMSFHGWYRRKIITLDGTTTRIPIARYRCS
NCRKTHAILPDFVAPYRHYSQVLIAAVVEEVVSKQVPPERVEGNQDIPTTRRWIRRFLKRCHEVIGVLESIAFRYTGQIKSLLTEARSSPWEQMLAALEL
LPAVKATSVFGAVNLWLTMDVVGLWL

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1347 bp	448 aa	835	2181	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVRLPKELSYQQEIALKRFALIAPLLEPDLEAAEKRQRRKEILARSEISSRTLRRYLQLYRQQGLSGLMPKIRSDNGSSRTISHEIIEEAVKLKEELPER
SVSQIIAILEGEKKVPAGMLARSTLGRHLSRLGLTQKEANQKISGHRRFAKEQRNRLWQADIKYGPYLPHPKNPKRKVRTYLVAFIDDATRLLCHGEFYL
DQKRPVLEDCFRKAILKRGIPDAVYVDNGKIFVSRWFRLGCARLGIRPINTKPYSPESKGKIERFNRTVESFIAEIELQQPETLAELNQAFAVWVEEGYN
HHPHSSLENETPANRFQKDTRRLRFASLEECREAFLWEASRRVDKTGCIKLEGRFYEIGLEWIRKTVDLRYDPFDLESIEFWYNGQKQGLAKPLVIQEYN
NLAAKNKKEQENKTPKTSRLLTVLEERSIERRRRKLGAIPFRQLEGGK

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
804 bp	267 aa	2184	2987	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MFEHFFGFHRTPFGRDLPVNSLFTTPAHEELVSRLKYAAEKRLFALITGEVGAGKSTALRQLLSLLNPNKYRVIYISDSGLTPRHFYWEALRQLDCEPRF
NRGQAKRQLHQVILDLAENQQKIPVVIVDEAHLLDREMLEEIRFLLNFRMDSYNPMSLILVGQPELRRILQLQVYEAIAQRINLRYHLPPMEREEAKGYV
AHHLRTAGASSAIFTDDALDMVYEYAGGIARKINNLCIACLMAAAAEQKRLIDDRMVKVVIENEFSV

Blast result :

Comments

ISChy3 is 55% aa similar to ISBst1.
ORF1 and ORF3 are passenger genes.

References

1] Wu,M., Ren,Q., Durkin,A.S., Daugherty,S.C., Brinkac,L.M., Dodson,R.J., Madupu,R., Sullivan,S.A., Kolonay,J.F., Nelson,W.C., Tallon,L.J., Jones,K.M., Ulrich,L.E., Gonzalez,J.M., Zhulin,I.B., Robb,F.T. and Eisen,J.A. (2005) PLoS Genet. 1 (5), E65.