ISEca11
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004547 | ND | Erwinia carotovora | Erwinia carotovora subsp. atroseptica SCRI1043 |
DNA section
IS Length : 1211 bp
Ends
IR Length : 22/30
IRL : TGTAGTCCCCCCTAAATTTAGTCTTACCGCTTAATGGAGTTCTCTGGTTA
IRR : TGTAGTCCCTCCGGTTTTTAGTACCACCGCCAATGAGGATCTCCGGCCAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTTCACTGGTG | ATAG | CACTGGTAGCCT | 4 |
DNA sequence
TGTAGTCCCCCCTAAATTTAGTCTTACCGCTTAATGGAGTTCTCTGGTTAAAATACAACCAATGGAGGCTCATCATGAAGAAAGCGCGTTTCACTGAGAC
TCAGATCCTGCGGGTTTTGAAAGAAGTTGAAGGTGGTCGGCATGTGAAGGATGTGTGCCGTGAAAACGGTGTGTCGGAAGCCAGCTATTACAACTGGAAA
TCTAAATACGGCGGCATGGAGTCCTCTGATATCAAACGGATGAAAGAGTTGGAAGAGGAAAATCGCCGGTTAAAGCAAATGTACGCATCCCTGAGCTTAG
ATCATGAAATTCTTAAGGATGTTGTTTCAAAAAAACTTTAACGGTGCCTGAGAAGCGTGAACTGGTGCATTATGTTATGACGGAACATCAGGCCAGCGAA
CGACGCGGGTGCCGGATTATCGGTATCAGTCGAAGTCTGTTGCATTATTGCCCGAATACGACGCGGGATCTGCCTGTAGTCGAGGCGCTACAAAAACTGG
CGCATCAGTATCCGGCCTATGGTTTTGGACTTATGTTCAATAAGTTACGACAGGCAGGGTTACTGTGGAATGCAAAGCGGGTTTACCGGATCTATCGATT
GTTGAAACTCAACTTTCGGCGCAAAGGGAAAAAACGTTTACCTAACAGGCATCCACAGCCGTTAGTTATACCGCATAAAATGAACCACTGTTGGTCAGTT
GATTTTATGAGTGATGCCTTGAGCGACGGGCGGCGATTCAGGTTATTTAACGTCGTTGATGACTTTAATCGGGAAGCGCTGGCCATCGATGTTGATTTGA
ATATACCGGCTCATCGTGTTGTGCGAATTCTGGAACGATTAAGCGCAGAACGAGGCTACCCGGCTTTTATACGAAGTGATAATGGGCCAGAGCTCACCGC
CGCAGTCTTAGCCGAATGGGCAGAACGCCACGGTGTGAAACTCGATTTTATTCAGCCCGGAAAGCCGATGCAGAACGGATTTATCGAGCGATTTAACAAA
ACGCTACGAACAGAAATACTCGATATGTATCTGTTCAGAACCTTGTCTGAAGTGCGTGAACTCACAGAGGACTGGCGCACAGAATATAACGAAGAACGCC
CGCATAGCTCACTGGGTGATATGCCACCTGTTATCTATGCGCGGCAAAAACTGGCCGGAGATCCTCATTGGCGGTGGTACTAAAAACCGGAGGGACTACA
TCAGATCCTGCGGGTTTTGAAAGAAGTTGAAGGTGGTCGGCATGTGAAGGATGTGTGCCGTGAAAACGGTGTGTCGGAAGCCAGCTATTACAACTGGAAA
TCTAAATACGGCGGCATGGAGTCCTCTGATATCAAACGGATGAAAGAGTTGGAAGAGGAAAATCGCCGGTTAAAGCAAATGTACGCATCCCTGAGCTTAG
ATCATGAAATTCTTAAGGATGTTGTTTCAAAAAAACTTTAACGGTGCCTGAGAAGCGTGAACTGGTGCATTATGTTATGACGGAACATCAGGCCAGCGAA
CGACGCGGGTGCCGGATTATCGGTATCAGTCGAAGTCTGTTGCATTATTGCCCGAATACGACGCGGGATCTGCCTGTAGTCGAGGCGCTACAAAAACTGG
CGCATCAGTATCCGGCCTATGGTTTTGGACTTATGTTCAATAAGTTACGACAGGCAGGGTTACTGTGGAATGCAAAGCGGGTTTACCGGATCTATCGATT
GTTGAAACTCAACTTTCGGCGCAAAGGGAAAAAACGTTTACCTAACAGGCATCCACAGCCGTTAGTTATACCGCATAAAATGAACCACTGTTGGTCAGTT
GATTTTATGAGTGATGCCTTGAGCGACGGGCGGCGATTCAGGTTATTTAACGTCGTTGATGACTTTAATCGGGAAGCGCTGGCCATCGATGTTGATTTGA
ATATACCGGCTCATCGTGTTGTGCGAATTCTGGAACGATTAAGCGCAGAACGAGGCTACCCGGCTTTTATACGAAGTGATAATGGGCCAGAGCTCACCGC
CGCAGTCTTAGCCGAATGGGCAGAACGCCACGGTGTGAAACTCGATTTTATTCAGCCCGGAAAGCCGATGCAGAACGGATTTATCGAGCGATTTAACAAA
ACGCTACGAACAGAAATACTCGATATGTATCTGTTCAGAACCTTGTCTGAAGTGCGTGAACTCACAGAGGACTGGCGCACAGAATATAACGAAGAACGCC
CGCATAGCTCACTGGGTGATATGCCACCTGTTATCTATGCGCGGCAAAAACTGGCCGGAGATCCTCATTGGCGGTGGTACTAAAAACCGGAGGGACTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 75 | 341 | + | No |
Description : First part of the transposase
ORF sequence :
MKKARFTETQILRVLKEVEGGRHVKDVCRENGVSEASYYNWKSKYGGMESSDIKRMKELEEENRRLKQMYASLSLDHEILKDVVSKKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 864 bp | 288 aa | 317 | 1180 | + | No |
Description : Second part of the transposase
ORF sequence :
GCCFKKTLTVPEKRELVHYVMTEHQASERRGCRIIGISRSLLHYCPNTTRDLPVVEALQKLAHQYPAYGFGLMFNKLRQAGLLWNAKRVYRIYRLLKLNF
RRKGKKRLPNRHPQPLVIPHKMNHCWSVDFMSDALSDGRRFRLFNVVDDFNREALAIDVDLNIPAHRVVRILERLSAERGYPAFIRSDNGPELTAAVLAE
WAERHGVKLDFIQPGKPMQNGFIERFNKTLRTEILDMYLFRTLSEVRELTEDWRTEYNEERPHSSLGDMPPVIYARQKLAGDPHWRWY
RRKGKKRLPNRHPQPLVIPHKMNHCWSVDFMSDALSDGRRFRLFNVVDDFNREALAIDVDLNIPAHRVVRILERLSAERGYPAFIRSDNGPELTAAVLAE
WAERHGVKLDFIQPGKPMQNGFIERFNKTLRTEILDMYLFRTLSEVRELTEDWRTEYNEERPHSSLGDMPPVIYARQKLAGDPHWRWY
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1106 bp | 369 aa | 75 | 1180 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKARFTETQILRVLKEVEGGRHVKDVCRENGVSEASYYNWKSKYGGMESSDIKRMKELEEENRRLKQMYASLSLDHEILKDVVSKKTLTVPEKRELVHY
VMTEHQASERRGCRIIGISRSLLHYCPNTTRDLPVVEALQKLAHQYPAYGFGLMFNKLRQAGLLWNAKRVYRIYRLLKLNFRRKGKKRLPNRHPQPLVIP
HKMNHCWSVDFMSDALSDGRRFRLFNVVDDFNREALAIDVDLNIPAHRVVRILERLSAERGYPAFIRSDNGPELTAAVLAEWAERHGVKLDFIQPGKPMQ
NGFIERFNKTLRTEILDMYLFRTLSEVRELTEDWRTEYNEERPHSSLGDMPPVIYARQKLAGDPHWRWY
VMTEHQASERRGCRIIGISRSLLHYCPNTTRDLPVVEALQKLAHQYPAYGFGLMFNKLRQAGLLWNAKRVYRIYRLLKLNFRRKGKKRLPNRHPQPLVIP
HKMNHCWSVDFMSDALSDGRRFRLFNVVDDFNREALAIDVDLNIPAHRVVRILERLSAERGYPAFIRSDNGPELTAAVLAEWAERHGVKLDFIQPGKPMQ
NGFIERFNKTLRTEILDMYLFRTLSEVRELTEDWRTEYNEERPHSSLGDMPPVIYARQKLAGDPHWRWY
Blast result :
Comments
ISEca11 is 93 % (ORFA) aa similar to ISD1 and 73% (ORFB) aa similar to IS1351.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Bell,K.S., Sebaihia,M., Pritchard,L., Holden,M.T., Hyman,L.J., Holeva,M.C., Thomson,N.R., Bentley,S.D., Churcher,L.J., Mungall,K., Atkin,R., Bason,N., Brooks,K., Chillingworth,T., Clark,K., Doggett,J., Fraser,A., Hance,Z., Hauser,H., Jagels,K., Moule,S., Norbertczak,H., Ormond,D., Price,C., Quail,M.A., Sanders,M., Walker,D., Whitehead,S., Salmond,G.P., Birch,P.R., Parkhill,J. and Toth,I.K. (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (30), 11105-11110.