ISEc27
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY857617 | ND | Escherichia coli | Escherichia coli BEN2908 |
DNA section
IS Length : 1321 bp
Ends
IR Length : 15/22
IRL : TGGCCCCCTGAATCTCCAGACAACCAGTATCACTTAAATAAGTGATAGTC
IRR : TGGCCCCTATATTTCCATACACTTTTTATCACTTAACCCATTACTGGTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATAAATAGAC | ATCCAATTGC | 0 |
DNA sequence
TGGCCCCCTGAATCTCCAGACAACCAGTATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTT
AGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAA
CATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATACCAGGAAGGAAGTCTTACTGCTGTCGCCGCCGGAGAACAGGTTGTTCCTGCCTCTG
AACTTGCTGCCGCCATGAAGCAGATTAAAGAACTCCAGCGCCTGCTCGGCAAGAAAACGATGGAAAATGAACTCCTCAAAGAAGCCGTTGAATATGGACG
GGCAAAAAAGTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAAGCTTAGTCAGCCGTTGTCTCCGGGTCTCACGTGCGCAACTGCATGCCA
TGGCCCGTCGGTCGAAGGACTGGCAGGATCGTCGGTGCAAGCGCAAGCCTGATGATACTGACGCGCTGGCCCGTATCCATACCGTTATCGGCGATCTGCC
CACCTATGGTTATCGTCGGGTATGGGCACTGCTGCGCAGACAATCAGAAACTGACGACATGGCGGTGATCAATGCCAAACGCGTATACCGCATCATGCGT
CAGAATGCGCTGCTGCTTGAGCGTAAACCGGAAATACCGCCATCGAAGCGGGCGCATACAGGGAAAGTGGCCGTTGGAGAAAGTAACCAGCGGTGGTGCT
CTGACGGCTTCGAGTTCAGCTGTGATAACGGTGAAAAACTGCGGGTCACGTTCGCTCTGGACTGTTGCGATCGCGAGGCACTTTACTGGGCGGCCAGTAA
CGGTGGATATGACAGTGAAACCGTGCAGGACGTCATGCTGGGTGCCGTGGAGCGTCGTTTCGGTAACAGCCTGCCGACATCCCCAGTTGAGTGGCTGACA
GACAACGGTTCAGCCTACCGTTCTTATCAGACGCGTCAGTTCGCCAGAATGGTAGGACTGGAGCCTAAACATACGGCGGTACGTAGCCCGGAAAGCAACG
GGATGGCAGAGAGCTTCGTGAAAACGATGAAGCGCGATTACATCAGCATCATGCCGAAACCCGACGGGTTAACAGCGGTAAAGAACCTTGCGGAGGCCTT
CGAACATTACAACGAATGGCATCCGCATAGTGCACTGGGGTATCGTTCGCCACGGGAATATCTGCGGCGGCGAACCAGTAATGGGTTAAGTGATAAAAAG
TGTATGGAAATATAGGGGCCA
AGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAA
CATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATACCAGGAAGGAAGTCTTACTGCTGTCGCCGCCGGAGAACAGGTTGTTCCTGCCTCTG
AACTTGCTGCCGCCATGAAGCAGATTAAAGAACTCCAGCGCCTGCTCGGCAAGAAAACGATGGAAAATGAACTCCTCAAAGAAGCCGTTGAATATGGACG
GGCAAAAAAGTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAAGCTTAGTCAGCCGTTGTCTCCGGGTCTCACGTGCGCAACTGCATGCCA
TGGCCCGTCGGTCGAAGGACTGGCAGGATCGTCGGTGCAAGCGCAAGCCTGATGATACTGACGCGCTGGCCCGTATCCATACCGTTATCGGCGATCTGCC
CACCTATGGTTATCGTCGGGTATGGGCACTGCTGCGCAGACAATCAGAAACTGACGACATGGCGGTGATCAATGCCAAACGCGTATACCGCATCATGCGT
CAGAATGCGCTGCTGCTTGAGCGTAAACCGGAAATACCGCCATCGAAGCGGGCGCATACAGGGAAAGTGGCCGTTGGAGAAAGTAACCAGCGGTGGTGCT
CTGACGGCTTCGAGTTCAGCTGTGATAACGGTGAAAAACTGCGGGTCACGTTCGCTCTGGACTGTTGCGATCGCGAGGCACTTTACTGGGCGGCCAGTAA
CGGTGGATATGACAGTGAAACCGTGCAGGACGTCATGCTGGGTGCCGTGGAGCGTCGTTTCGGTAACAGCCTGCCGACATCCCCAGTTGAGTGGCTGACA
GACAACGGTTCAGCCTACCGTTCTTATCAGACGCGTCAGTTCGCCAGAATGGTAGGACTGGAGCCTAAACATACGGCGGTACGTAGCCCGGAAAGCAACG
GGATGGCAGAGAGCTTCGTGAAAACGATGAAGCGCGATTACATCAGCATCATGCCGAAACCCGACGGGTTAACAGCGGTAAAGAACCTTGCGGAGGCCTT
CGAACATTACAACGAATGGCATCCGCATAGTGCACTGGGGTATCGTTCGCCACGGGAATATCTGCGGCGGCGAACCAGTAATGGGTTAAGTGATAAAAAG
TGTATGGAAATATAGGGGCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 411 bp | 136 aa | 42 | 452 | + | No |
Description : First part of the transposase
ORF sequence :
MIVLILVFRLVIGEQMIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQR
LLGKKTMENELLKEAVEYGRAKKWIAHAPLLPGDGE
LLGKKTMENELLKEAVEYGRAKKWIAHAPLLPGDGE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 392 | 1315 | + | No |
Description : Second part of the transposase
ORF sequence :
IWTGKKVDSARALIARGWGVSLVSRCLRVSRAQLHAMARRSKDWQDRRCKRKPDDTDALARIHTVIGDLPTYGYRRVWALLRRQSETDDMAVINAKRVYR
IMRQNALLLERKPEIPPSKRAHTGKVAVGESNQRWCSDGFEFSCDNGEKLRVTFALDCCDREALYWAASNGGYDSETVQDVMLGAVERRFGNSLPTSPVE
WLTDNGSAYRSYQTRQFARMVGLEPKHTAVRSPESNGMAESFVKTMKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALGYRSPREYLRRRTSNGLS
DKKCMEI
IMRQNALLLERKPEIPPSKRAHTGKVAVGESNQRWCSDGFEFSCDNGEKLRVTFALDCCDREALYWAASNGGYDSETVQDVMLGAVERRFGNSLPTSPVE
WLTDNGSAYRSYQTRQFARMVGLEPKHTAVRSPESNGMAESFVKTMKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALGYRSPREYLRRRTSNGLS
DKKCMEI
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1274 bp | 424 aa | 42 | 1315 | + | Yes |
Chemistry : DDE
ORF sequence :
MIVLILVFRLVIGEQMIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQR
LLGKKTMENELLKEAVEYGRAKKVDSARALIARGWGVSLVSRCLRVSRAQLHAMARRSKDWQDRRCKRKPDDTDALARIHTVIGDLPTYGYRRVWALLRR
QSETDDMAVINAKRVYRIMRQNALLLERKPEIPPSKRAHTGKVAVGESNQRWCSDGFEFSCDNGEKLRVTFALDCCDREALYWAASNGGYDSETVQDVML
GAVERRFGNSLPTSPVEWLTDNGSAYRSYQTRQFARMVGLEPKHTAVRSPESNGMAESFVKTMKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALG
YRSPREYLRRRTSNGLSDKKCMEI
LLGKKTMENELLKEAVEYGRAKKVDSARALIARGWGVSLVSRCLRVSRAQLHAMARRSKDWQDRRCKRKPDDTDALARIHTVIGDLPTYGYRRVWALLRR
QSETDDMAVINAKRVYRIMRQNALLLERKPEIPPSKRAHTGKVAVGESNQRWCSDGFEFSCDNGEKLRVTFALDCCDREALYWAASNGGYDSETVQDVML
GAVERRFGNSLPTSPVEWLTDNGSAYRSYQTRQFARMVGLEPKHTAVRSPESNGMAESFVKTMKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALG
YRSPREYLRRRTSNGLSDKKCMEI
Blast result :
Comments
ISEc27 is 100% (ORFA) and 90% (ORFB) aa similar to IS2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chouikha,I., Germon,P., Bree,A., Gilot,P., Moulin-Schouleur,M. and Schouler,C. (2006) J. Bacteriol. 188 (3), 977-987.