ISAeca1

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
EF118171 ND Aeromonas caviae
Aeromonas media WS
Aeromonas caviae
Aeromonas punctata plasmid pJDB2
Haemophilus influenzae plasmid ICEhin1056
Aeromonas punctata Sch3
DNA section
IS Length : 1091 bp

Ends


IR Length : 22/29

IRL : CGTGAATTCAGATAATAAGTGCAATGCCCCTGGTGCATAGGAGAGAGGCG
IRR : CGCGAATTCAACTTCGAAGTGCAATTCCCGATTAAAAATCAGGCTGCTTT

Insertion site


Left flankDirect repeatRight flankDR Length
TCTTCGCGGAACTCCATGGAGA2
CTATTAGTAGACCAGCTAGAGA2

DNA sequence

CGTGAATTCAGATAATAAGTGCAATGCCCCTGGTGCATAGGAGAGAGGCGGTCAACTACCAGTTAGGGTACGCTTCTTCTCGCCAAAGAAACAAAGCGAT
ACACCATGAAATACCAGCAGTTGACCGAGGGGCTACGATACCAGATTGCCTGCCTCCGTGACCATGGCCTGTCACAGGCCCGCATTGCCAAACAGCTCGG
GGTTCATCCATCGACTATCAGTCGTGAGCTTCGTCGTAATTGTGCTGGGCAGGCCTATCAGCCGCAGCAGGCTCACCAACTTGCCACGGCACGACGGCTG
ACGAGTCAGAAGCGTACTGTCCCGTCTGATACCATCGACTTTGTTTGCATGGCGCTGGTCGCGGACTGGAGTCCCGAGCAAATCAGTGGCGTTGGAGACA
TGATTAACTGCCCGGTTAGCCACGAGTGGATATACCGCTATGTGGCACGCGACAAGGCCAACGGTGGCACGCTATACCGGCATCTGCGTCAGGGTCACAA
ACGCTATCGCAAGGGTAAGAACAGCAAACGCAGTGTCATTCCCAATCCGGTATCGATTGATGAACGCCCTGCGATAGTCAATGAGCGGAGCCGTGTGGGA
GATTGGGAAGTCGATACCGTGTTGGGTAAGCAAGGCAGCGGTGCAATAGTGAGTCTGTTGGAGCGTAAGAGCCGGCTCTACCTGGTTCAATACGTTCCCA
GCAAAACAGCCAGCGCGGTGGCGGACACCATTATCAGCATGCTGAAACCCTACCAGGCACATGTCCATACCATCACAGCTGACAATGGTAGCGAGTTTGT
CGAACATGAACGTGTTGCAGCAGAGCTGGAAGCGCAGGTGTACTTTGCGCATCCATACAGTGCATGGGAACGGGGACAGAATGAGAATAGCAATGGTCTG
CTGCGGCAATATGTGCCCAAAGGCAGTGATTTGAGTGTGGTGACAGCAGAGGCACTGTCAGCCGTCGAAAAGCGGCTGAACTTCAGGCCGCGCAAATGCT
TGGGATTTAAACAGCCACAGGTGGTTTTTGACGAACTTCTGAAAGCAGCCTGATTTTTAATCGGGAATTGCACTTCGAAGTTGAATTCGCG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
948 bp315 aa1061053+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKYQQLTEGLRYQIACLRDHGLSQARIAKQLGVHPSTISRELRRNCAGQAYQPQQAHQLATARRLTSQKRTVPSDTIDFVCMALVADWSPEQISGVGDMI
NCPVSHEWIYRYVARDKANGGTLYRHLRQGHKRYRKGKNSKRSVIPNPVSIDERPAIVNERSRVGDWEVDTVLGKQGSGAIVSLLERKSRLYLVQYVPSK
TASAVADTIISMLKPYQAHVHTITADNGSEFVEHERVAAELEAQVYFAHPYSAWERGQNENSNGLLRQYVPKGSDLSVVTAEALSAVEKRLNFRPRKCLG
FKQPQVVFDELLKAA

 

Blast result :
Comments
ISAeca1 is 72% aa similar to ISAs2.
Additionnal Accession number : AJ627386 , AF126256 .
References
[1] Neuwirth C., Siebor E., Robin F. and Bonnet R. (2007) Antimicrob. Agents Chemother.