ISAzo8

  • Family ISAs1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008702 ND Azoarcus sp.
Azoarcus sp. BH72
DNA section
IS Length : 1261 bp

Ends


IR Length : 21/25

IRL : CAGGGCTATCGCACCTACCTGACATAGCCACATGCAGAATCTCGTTCACC
IRR : CAGGGCAATCGCACCTTCATGTCATGAACCCGAGCAATTCAGCGCGGAAC

Insertion site


Left flankDirect repeatRight flankDR Length
ACTCCGGTAGGTCACTGTAGTTGTCTGATCTGAG10

DNA sequence

CAGGGCTATCGCACCTACCTGACATAGCCACATGCAGAATCTCGTTCACCTTCATGTGCTTACGACACCCCTGTTCACAGTCCGCGAAATTGTGTAGTTT
CCTCGTCTTTTGGGGTTCCCATGCAGACGAGGGAGGTGATGCCGCTCACGCAGGTGTTCGTCTCGATCTCCGACCCGCGCAGCGCCCGGCGGGTCCGGCA
CGATCTGTCCGAGTTGCTCACGGTAGCGGTGTGCGCGGTGCTGTGCAGCGCGGACGAGTTCTCCGACATCGAGGCGTGGGCCAAGGAGCGCATCGACTGG
CTGCGAGGCTTTCTGGTGCTTGAGCACGGCATCCCCTCGCACGACACGTTCGGTCGGGTGTTCGCGGCGCTCAACCCGCGCGAGTTCGAAGCCGCCTTTC
GGCGCTGGGTCGGGCAACTGGTGCCGGCCCTGGGCCCGGATGCGGTGGTGGCCATCGACGGCAAGACCAGCCGGCGCAGCACGACCAAGGCCGCGGCCAA
TCCCTTGCACCTGGTCAGCGCCTTCGCCGCCGACGTCGGCCTGGTGCTCGGTCAGACGGCGACGGCCGAGAAGTCCAATGAGATCACGGCGATTCCCGAG
TTGCTGGCGACGCTGGCGTTGCAGGGCTGTGTCGTCACCATCGATGCGATGGGCACCCAGACCGGCATTGCGAAGGCGATTCGCGACGGCGGTGCCGACT
ACGTGCTGTGCGTGAAGGACAACCACCCGAAGCTCGTCGAATCGTTCCTGCTCGCCCAGGCAGGTGTGGGGGGGCCACTGAGGCCCAGCTCCACGAGTGA
AAGCTGCGAGGACGGTCATGGCCGAACGGAAGTGCGCCGTTGCTGGGCGTTCGACGCGGTCGATCGGCTCTACAAGGCCGAACAGTGGCCGGATCTGAAG
AGCTTCGCGATCGTCGAGTGTGAGCGAACGGTCGGCACCAAGACCAGTTGTGAGCGCCGCTACTACATCAGCTCCCTGCCGGCGGAGGCGACCCGGATCG
CCCATGCGGTGCGTAGCCACTGGGAGGTCGAGAACCGGCTTCATTGGTGCCTCGACGTCCTGTTCAACGAGGACCAGTCCACCGTGCGCACCGGATTCGC
GGCCAACAACCTCGCCATCGTTCGCCACATCGTGATGAACCTGCTTCGGCTGAACACTTCGCGCAAGGGCAGCATCAAGACGAAGCGGATGCTCGCCGCC
ACCTCCGACACGTTCCGCGCTGAATTGCTCGGGTTCATGACATGAAGGTGCGATTGCCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1125 bp374 aa1211245+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MQTREVMPLTQVFVSISDPRSARRVRHDLSELLTVAVCAVLCSADEFSDIEAWAKERIDWLRGFLVLEHGIPSHDTFGRVFAALNPREFEAAFRRWVGQL
VPALGPDAVVAIDGKTSRRSTTKAAANPLHLVSAFAADVGLVLGQTATAEKSNEITAIPELLATLALQGCVVTIDAMGTQTGIAKAIRDGGADYVLCVKD
NHPKLVESFLLAQAGVGGPLRPSSTSESCEDGHGRTEVRRCWAFDAVDRLYKAEQWPDLKSFAIVECERTVGTKTSCERRYYISSLPAEATRIAHAVRSH
WEVENRLHWCLDVLFNEDQSTVRTGFAANNLAIVRHIVMNLLRLNTSRKGSIKTKRMLAATSDTFRAELLGFMT

 

Blast result :
Comments
ISAzo8 is 78% aa similar to ISAzo3
References
1] Krause,A., Ramakumar,A., Bartels,D., Battistoni,F., Bekel,T., Boch,J., Bohm,M., Friedrich,F., Hurek,T., Krause,L., Linke,B., McHardy,A.C., Sarkar,A., Schneiker,S., Syed,A.A., Thauer,R., Vorholter,F.J., Weidner,S., Puhler,A., Reinhold-Hurek,B., Kaiser,O. and Goesmann,A. (2006)Nat. Biotechnol. 24 (11), 1385-1391