ISEc25
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Escherichia coli | Escherichia coli O152:H28 SE11 plasmid 3 |
DNA section
IS Length : 1310 bp
Ends
IR Length : 11/13
IRL : TGAATCGCCCCGGGAATCCTGGAGACTAAACTCCTTGAGAGAGAGGTAAA
IRR : TGAACCGCCCCTGTTTTCCGGGAGAGTGTTTTGTCTGTGAACTCAGGCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCAGACACTG | ATCCGTAATA | 0 |
DNA sequence
TGAATCGCCCCGGGAATCCTGGAGACTAAACTCCTTGAGAGAGAGGTAAACAGGATGACTAAAAATATGCGCTTTTCCCCTGAGGTCCGTCAGCGAGCCG
TTCGTATGGTTATCGAAAGCCAGAATGACTATGACTCGCAGTGGGCGGCCATCAGCTCCATCGCCCCTAAAATCGGCTGTACGCCTGAGACCCTGCGTAC
CTGGCTGCGTCAGTATGAGCGCGATGCCGGTGCAGGTGACGGCGGACTGAGCACCACAGAACGCCAGCGTCTGAAGGAACTGGAGCGTGAAAACCGCGAA
CTGCGCCGCAGCAACGATATCCTGCGCCAGGCTTCCGCGTATTTTGCGAAGGCGGAGTTCGACCGCCTGTGGAAAAAATAGCGCCTCTGGTGGATACCCT
GCGTGGTACGCACGGGGTCGGACCGGTATGCCACGAGCTGGCTGTCGCCCCGTCAACGTATTACCGGCATCGTGCGCATCAACGGCATCCGGATAAACGC
AGTCAGCGGGCACAGCGTGATGACCTGCTGAAGCCGGAGATACAGCGCGTGTACGATGTAAACCACAGCGTGTACGGTGTGCGCAAGGTCTGGCGCCAGT
TGTTGCGTGAGAGGGTGACCGTAGCCAGGTGCACAGTAGAACGGCTGATGAAGGTGATGGGGCTTGCCGGTGTGCGCCGGGGTAAAAAGGTCCGCACCAC
AGTCAGCCGGAAAGAAGCCGCTGCGGGTGACCGGGTGAACCGCCAGTTCGTGGCAGAATGCCCCAACCAGCTGTGGGTGGCTGACTTCACGTATGTGAGC
ACCTGGCAGGGCTTCGCTTACGTGGCGTTCATCATCGATGTGTTCGCCGGCGTCATTGTGGGCTGGCGGGTCTCATCGTCGATGAAAACGACGTTCGTGC
TGGATGCACTGGAGCAGGCGCTGTGGGCCCGTCGCCCGTCGGGCACCATCCATCACTCAGATAAGGGGTCACAATATGTGTCGCTGGCATACACTCAGCG
GCTTAAGGATGCAGAGTTGCTGGCGTCAACCGGCAGCACAGGCGACTCATATGACAACGCGATGGCGGAGAGCATCAACGGACTTTATAAAGCGGAGGTG
ATACATCGTAAGAGCTGGAAAAACCGCGCCGAAGTGGAACTGGCCACACTGACGTGGGTGGACTGGTACAATAACCGACGGTTGCTGGGACAACTGGGTC
ATATCCCGCCGGCAGAAGCGGAAAAGGCATATTACGCTTCCATCGGAAATGGTGATCTGGCAGCCTGAGTTCACAGACAAAACACTCTCCCGGAAAACAG
GGGCGGTTCA
TTCGTATGGTTATCGAAAGCCAGAATGACTATGACTCGCAGTGGGCGGCCATCAGCTCCATCGCCCCTAAAATCGGCTGTACGCCTGAGACCCTGCGTAC
CTGGCTGCGTCAGTATGAGCGCGATGCCGGTGCAGGTGACGGCGGACTGAGCACCACAGAACGCCAGCGTCTGAAGGAACTGGAGCGTGAAAACCGCGAA
CTGCGCCGCAGCAACGATATCCTGCGCCAGGCTTCCGCGTATTTTGCGAAGGCGGAGTTCGACCGCCTGTGGAAAAAATAGCGCCTCTGGTGGATACCCT
GCGTGGTACGCACGGGGTCGGACCGGTATGCCACGAGCTGGCTGTCGCCCCGTCAACGTATTACCGGCATCGTGCGCATCAACGGCATCCGGATAAACGC
AGTCAGCGGGCACAGCGTGATGACCTGCTGAAGCCGGAGATACAGCGCGTGTACGATGTAAACCACAGCGTGTACGGTGTGCGCAAGGTCTGGCGCCAGT
TGTTGCGTGAGAGGGTGACCGTAGCCAGGTGCACAGTAGAACGGCTGATGAAGGTGATGGGGCTTGCCGGTGTGCGCCGGGGTAAAAAGGTCCGCACCAC
AGTCAGCCGGAAAGAAGCCGCTGCGGGTGACCGGGTGAACCGCCAGTTCGTGGCAGAATGCCCCAACCAGCTGTGGGTGGCTGACTTCACGTATGTGAGC
ACCTGGCAGGGCTTCGCTTACGTGGCGTTCATCATCGATGTGTTCGCCGGCGTCATTGTGGGCTGGCGGGTCTCATCGTCGATGAAAACGACGTTCGTGC
TGGATGCACTGGAGCAGGCGCTGTGGGCCCGTCGCCCGTCGGGCACCATCCATCACTCAGATAAGGGGTCACAATATGTGTCGCTGGCATACACTCAGCG
GCTTAAGGATGCAGAGTTGCTGGCGTCAACCGGCAGCACAGGCGACTCATATGACAACGCGATGGCGGAGAGCATCAACGGACTTTATAAAGCGGAGGTG
ATACATCGTAAGAGCTGGAAAAACCGCGCCGAAGTGGAACTGGCCACACTGACGTGGGTGGACTGGTACAATAACCGACGGTTGCTGGGACAACTGGGTC
ATATCCCGCCGGCAGAAGCGGAAAAGGCATATTACGCTTCCATCGGAAATGGTGATCTGGCAGCCTGAGTTCACAGACAAAACACTCTCCCGGAAAACAG
GGGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 327 bp | 108 aa | 55 | 381 | + | No |
Description : First part of the transposase
ORF sequence :
MTKNMRFSPEVRQRAVRMVIESQNDYDSQWAAISSIAPKIGCTPETLRTWLRQYERDAGAGDGGLSTTERQRLKELERENRELRRSNDILRQASAYFAKA
EFDRLWKK
EFDRLWKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 978 bp | 325 aa | 291 | 1268 | + | No |
Description : Second part of the transposase
ORF sequence :
KPRTAPQQRYPAPGFRVFCEGGVRPPVEKIAPLVDTLRGTHGVGPVCHELAVAPSTYYRHRAHQRHPDKRSQRAQRDDLLKPEIQRVYDVNHSVYGVRKV
WRQLLRERVTVARCTVERLMKVMGLAGVRRGKKVRTTVSRKEAAAGDRVNRQFVAECPNQLWVADFTYVSTWQGFAYVAFIIDVFAGVIVGWRVSSSMKT
TFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKDAELLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLG
QLGHIPPAEAEKAYYASIGNGDLAA
WRQLLRERVTVARCTVERLMKVMGLAGVRRGKKVRTTVSRKEAAAGDRVNRQFVAECPNQLWVADFTYVSTWQGFAYVAFIIDVFAGVIVGWRVSSSMKT
TFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKDAELLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLG
QLGHIPPAEAEKAYYASIGNGDLAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1214 bp | 404 aa | 55 | 1268 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKNMRFSPEVRQRAVRMVIESQNDYDSQWAAISSIAPKIGCTPETLRTWLRQYERDAGAGDGGLSTTERQRLKELERENRELRRSNDILRQASAYFAKA
EFDRLWKKIAPLVDTLRGTHGVGPVCHELAVAPSTYYRHRAHQRHPDKRSQRAQRDDLLKPEIQRVYDVNHSVYGVRKVWRQLLRERVTVARCTVERLMK
VMGLAGVRRGKKVRTTVSRKEAAAGDRVNRQFVAECPNQLWVADFTYVSTWQGFAYVAFIIDVFAGVIVGWRVSSSMKTTFVLDALEQALWARRPSGTIH
HSDKGSQYVSLAYTQRLKDAELLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGQLGHIPPAEAEKAYYASIGNG
DLAA
EFDRLWKKIAPLVDTLRGTHGVGPVCHELAVAPSTYYRHRAHQRHPDKRSQRAQRDDLLKPEIQRVYDVNHSVYGVRKVWRQLLRERVTVARCTVERLMK
VMGLAGVRRGKKVRTTVSRKEAAAGDRVNRQFVAECPNQLWVADFTYVSTWQGFAYVAFIIDVFAGVIVGWRVSSSMKTTFVLDALEQALWARRPSGTIH
HSDKGSQYVSLAYTQRLKDAELLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGQLGHIPPAEAEKAYYASIGNG
DLAA
Blast result :
Comments
1 copy in E.coli SE11 plasmid3, plus 1 ruminant. An example of a complete ISEc25 is found in the E.coli SE15 plasmid3 genome sequence at co-ordinates 36035-37344 (complement).
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Tadasuke Ooka, Tetsuya Hayashi (2008) Direct submission.