ISCro6
- Family IS4
- Group IS4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Citrobacter rodentium | Citrobacter rodentium ICC168 |
DNA section
IS Length : 1415 bp
Ends
IR Length : 18
IRL : TAACACCGATCGGTTAAGCAAAATGATCGGTTGAACGATCCAGTGGGTAG
IRR : TAACACCGTTCACTTAAGATGTTTTGAGTGAACGGGATACTGGGTTTTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TAACACCGATCGGTTAAGCAAAATGATCGGTTGAACGATCCAGTGGGTAGGTGACAATAGCCTCATCAATTTTCTGATGGGGCTTTTTTATGCTTTCGAC
CTGGCTTGACGATACCCAGACCTGGGCTGAACCCGCTTCACTTTCCTGTTTTCAGCGTGCCATTCCCCTTGAGTGGATTTCTCAGGTCCTTGATTCCACC
AATAAAGCCAGTATCCGTAAACGTAAGCTCCCCGCAGAACTCGTGGTCTGGCTGATTGTGGGCATGGGCCTGTATCGCGACCGCTCCATCACGGACGTTG
TGACAAAACTCGATCTCGTCCTGAGTTCACAGGAAGGCGAGACTCTCGCCGCCAGCTCTGTTGCCCGGGCCCGTCAGAGACTCAGTGACGAACCGCTCCG
GGAGCTCTTCACCCTGACGGCCAGCCACTGGACGCAGCAGGAAGACAAGGATGACCTCTGGCACGGGCTGCGCCTGTTTGCCGTTGATGGCACGCTTTTT
CGTACTCCCGATACGCCCGAACTTGCTGAGCATTTTGAGTACATAAAGCATCGTCCGGACAGACACACCGAGTACCCGATGGTCAGGCTGTGCGCCATGA
TGTCACTGCGCAGCAGATTGATTCACGGCGTAAAATTCGGCCCGGCAAACACCGGAGAAGTCAGTTACGCGAAACAGTTATCGCCGCAGGCGAAGAGCCT
GACCCTGTTTGACCGATGTTATCTGTCCGCAGAGTTACTGATTAACTGGCAGCGCAGGCAGCAGGAAGCGCACTGGCTGGTTCCCCTGAAGGGCAATACA
AAATACCGCATAGTTGAAACCTTCGCCGGAGGAGACCATCTGGTGGAGATGCAGGTCTCGCCGCAGGCCCGCAAACAGGACAGTTCGCTGCCAGAAAACT
GGCAGGCCCGGCTAATAGAATATGAAGATGAATCAGGTGATTACAAAGGTTTTATCACCTCTCTGACAGAGCCGGGGCAGTATCCGGCAGAGGCGCTGCG
TTATGTATATCAGGAACGATGGAGCATAGAAAATGGTTATGGAGAACTGAAACAGTTCCAGCTGAGCACAGCCACGCTGTTGCGCAGTCAGAAAGTGAGC
GGGATTTACCAGGAAATATGGGGCCTGCTGACGGCCTATAATCTGATACGGATGGAGATGAGTCAGATAGCACGGGAAGCAAAAGTGCCGCCGCTGCGGA
TAAGCTTCGTGATGGCGATGAGACTAATCCAGGATGAACTTGTCTGGTGCTCACTGGGTAAACCGGGGACGATCCCAACGAAGCTAAAGAAAATGCGGGA
GAATGTGAAGCAATTTATCCTGCCAGAAAAGAGAAAACGGCCCAAAAAGAGGACCGTCAGAATCTCAAAAACCCAGTATCCCGTTCACTCAAAACATCTT
AAGTGAACGGTGTTA
CTGGCTTGACGATACCCAGACCTGGGCTGAACCCGCTTCACTTTCCTGTTTTCAGCGTGCCATTCCCCTTGAGTGGATTTCTCAGGTCCTTGATTCCACC
AATAAAGCCAGTATCCGTAAACGTAAGCTCCCCGCAGAACTCGTGGTCTGGCTGATTGTGGGCATGGGCCTGTATCGCGACCGCTCCATCACGGACGTTG
TGACAAAACTCGATCTCGTCCTGAGTTCACAGGAAGGCGAGACTCTCGCCGCCAGCTCTGTTGCCCGGGCCCGTCAGAGACTCAGTGACGAACCGCTCCG
GGAGCTCTTCACCCTGACGGCCAGCCACTGGACGCAGCAGGAAGACAAGGATGACCTCTGGCACGGGCTGCGCCTGTTTGCCGTTGATGGCACGCTTTTT
CGTACTCCCGATACGCCCGAACTTGCTGAGCATTTTGAGTACATAAAGCATCGTCCGGACAGACACACCGAGTACCCGATGGTCAGGCTGTGCGCCATGA
TGTCACTGCGCAGCAGATTGATTCACGGCGTAAAATTCGGCCCGGCAAACACCGGAGAAGTCAGTTACGCGAAACAGTTATCGCCGCAGGCGAAGAGCCT
GACCCTGTTTGACCGATGTTATCTGTCCGCAGAGTTACTGATTAACTGGCAGCGCAGGCAGCAGGAAGCGCACTGGCTGGTTCCCCTGAAGGGCAATACA
AAATACCGCATAGTTGAAACCTTCGCCGGAGGAGACCATCTGGTGGAGATGCAGGTCTCGCCGCAGGCCCGCAAACAGGACAGTTCGCTGCCAGAAAACT
GGCAGGCCCGGCTAATAGAATATGAAGATGAATCAGGTGATTACAAAGGTTTTATCACCTCTCTGACAGAGCCGGGGCAGTATCCGGCAGAGGCGCTGCG
TTATGTATATCAGGAACGATGGAGCATAGAAAATGGTTATGGAGAACTGAAACAGTTCCAGCTGAGCACAGCCACGCTGTTGCGCAGTCAGAAAGTGAGC
GGGATTTACCAGGAAATATGGGGCCTGCTGACGGCCTATAATCTGATACGGATGGAGATGAGTCAGATAGCACGGGAAGCAAAAGTGCCGCCGCTGCGGA
TAAGCTTCGTGATGGCGATGAGACTAATCCAGGATGAACTTGTCTGGTGCTCACTGGGTAAACCGGGGACGATCCCAACGAAGCTAAAGAAAATGCGGGA
GAATGTGAAGCAATTTATCCTGCCAGAAAAGAGAAAACGGCCCAAAAAGAGGACCGTCAGAATCTCAAAAACCCAGTATCCCGTTCACTCAAAACATCTT
AAGTGAACGGTGTTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1317 bp | 438 aa | 90 | 1406 | + | No |
Chemistry : DDE
ORF sequence :
MLSTWLDDTQTWAEPASLSCFQRAIPLEWISQVLDSTNKASIRKRKLPAELVVWLIVGMGLYRDRSITDVVTKLDLVLSSQEGETLAASSVARARQRLSD
EPLRELFTLTASHWTQQEDKDDLWHGLRLFAVDGTLFRTPDTPELAEHFEYIKHRPDRHTEYPMVRLCAMMSLRSRLIHGVKFGPANTGEVSYAKQLSPQ
AKSLTLFDRCYLSAELLINWQRRQQEAHWLVPLKGNTKYRIVETFAGGDHLVEMQVSPQARKQDSSLPENWQARLIEYEDESGDYKGFITSLTEPGQYPA
EALRYVYQERWSIENGYGELKQFQLSTATLLRSQKVSGIYQEIWGLLTAYNLIRMEMSQIAREAKVPPLRISFVMAMRLIQDELVWCSLGKPGTIPTKLK
KMRENVKQFILPEKRKRPKKRTVRISKTQYPVHSKHLK
EPLRELFTLTASHWTQQEDKDDLWHGLRLFAVDGTLFRTPDTPELAEHFEYIKHRPDRHTEYPMVRLCAMMSLRSRLIHGVKFGPANTGEVSYAKQLSPQ
AKSLTLFDRCYLSAELLINWQRRQQEAHWLVPLKGNTKYRIVETFAGGDHLVEMQVSPQARKQDSSLPENWQARLIEYEDESGDYKGFITSLTEPGQYPA
EALRYVYQERWSIENGYGELKQFQLSTATLLRSQKVSGIYQEIWGLLTAYNLIRMEMSQIAREAKVPPLRISFVMAMRLIQDELVWCSLGKPGTIPTKLK
KMRENVKQFILPEKRKRPKKRTVRISKTQYPVHSKHLK
Blast result :
Comments
5 copies of ISCro6 in the chromosome of Citrobacter rodentium (2 are intact, 1 has suffered a frameshift in the transposase, 1 has been disrupted by insertion of ISCro1, and 1 has suffered a frameshift in the transposase and also been disrupted by the insertion of ISCro1). Found at coordinates: 239713..241128 (frameshift); 702882..704126(ISCro1)706826..707003; 776435..777849; 2824957..2826371; cpl(3621778..3621829)(ISCro1)3818227..3819600 (frameshift).
ISCro6 is 64% aa similar to ISPhsp1.
ISCro6 is 64% aa similar to ISPhsp1.
References
1] Nicola Petty (The Wellcome Trust Sanger Institute) (2008) Direct submission.