ISfinder Mirror Site

ISApr4

Family IS1595
Group ISNwi1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_ABHC01000005	ND	Alpha proteobacterium	Alpha proteobacterium BAL199

DNA section

IS Length : 3691 bp

Ends

IR Length : 20/25

IRL : GGGGATTATCCCCTTGACGCAAGTACGACCCGGACCTATATACAGGGTGT
IRR : GGAGATTATCCCCTGAGAGCAAGTAGCGCGTAGCGCCTCGCCGCAGACTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CAGATAGATTGGCCT	TAATTTTT	TGCCCGTTC	8

DNA sequence

GGGGATTATCCCCTTGACGCAAGTACGACCCGGACCTATATACAGGGTGTCAACGGGAGGCACCCATGTCCGAGAAGTTCACAGTTCGCGATTTCTTCAA
GCGGTTCCCGGATGACGACGCGTGCCTCCAGCATGTGATGGAAGTCCGCTTCGGTGCCCGCCACGTTTGCGCGGCTTGCGGCGTCGAGAGCACCTTTCAC
CGGATCGCCAAGCGTAAGGCGTATGCCTGCGCCGCTTGTGGTGCCCACCTGTACCCGTGCGCCGGTACGATCTTTCAGGACAGCCGCACGTCCCTGCAGA
CCTGGTTCTACGTGATCTTCCTGTTCGTCACGACCCGGCACGGTGTGTCAGGCAAGGAGATCGAGCGGACGGTCGGCGTGACGTACAAGACCGCGTGGCG
GATGGGCCAGCAGATCCGTCAACTGATGGAGAAGGCTGACGGCTTCGACCTGATGCGCGGTCACGTCGAACTGGACGAGGCTTACGTCGGCGGCCATCGC
CCCGGCAAGCGCGGTCGCGGTGCGGCTGGCAAGACCATCGTCATGGGCATGAAAGAGCGTGGCGGCCGGATGTCGGCGGAAGTCATCCCGAACATCAAGA
AGGCCACCCTGCGCGAAGTAACCCTGCGCAACGTCGAGCCGGGCTCCATCGTGTCGACCGACGAACTGATGTCCTACGGTTTACTGGACGGCGACGGGTT
CAAGCACGGGACCGTGAAGCACGGCGCCAAGGAGTTCGCCTATTACGACTATCGGCACGACGCTACGCACCACACCAACAACGTCGAGTCGTTCTGGTGG
CTGTTCAAGCGCTCCATCGCGAGCACCCACATCCACGTCTCTCAGAAGTACATGAACCGGTATCTGGCCGAATTCACGTTCCGCTCGAACTATCGCCAGA
TGCGGAACGCGATGTTCGATCTGCTGATCGGGGCGGTGTAACCACCGTCTTGATCAGTCGATCGAATGAGGCGCGGTTAGCCACAACGCCCCGCGCATCC
TCCTGCGCCATGAACTCGTCTAATCGGCCCGTCCGACGGGCCTCTTCCAGAGTAAGTCTCATGATGCCTCCGCAAAGCGCCTGAATTGGTCGTCGGTCGC
CTGCATCGGGACGATGTTTGCGACATCGCCGACCATTTGGGCCTTCTCGCCAAGTTCATCGACTGATTTGACCACAGTGTTCAACGATATCGATTCATCA
ACTGCTCTAATGTCGCTAAACATCATGAACTTGTTTGACGCCGAGTTGGCGTGATTTGTCATATATTCGAATATCGCGCGCCTTCTGTTGGGGAACACGA
CCACGTTGTGTGCCGGCCACGCTACATGGCGACCAGAAATCTCCGCGCTCCGCGTGACGATTTTCTTCCCGAACACCTTAGCAATCCGGTCAAAAATCCG
TTCATTCTGGCTTCTGCTCTGAACGTCGGCAGCACGGCGTACAGCTTCGGCTGCGGCCCGGCTCGATGCGTTGCCAACACAGGCAACTGCCGATTCAAGG
CGCCCAATCGGCACCCACAATGCGAAGATCGCATAGCCGTCAAATCCGACGCTGAACTCATCTGCCGCCTTAGCCGCAGACGACCCATAGAACTCTTCGG
CCGCCGACAGTTCCGCTTCGACAAGCCCGTAGCCCATATCGGACACAAGAACCTTGTCGCCGTTCCGCTCCACCTGCACAACGACCATGGCGCCGCTCGG
ATACATGATCGGCAGGTCGACGACGACGCGCCCATCCATTTCGCGCGCGCCTGCGAGCCGCACCACGGCTTCCCGGACGCGCTCAATAAGTACCTGAGTG
CTCGTCGTGATGTTCATAGCAAGCGCCCCTGATCTCCAGGGTTGGGGATGTCGCTCCCATTTATGATGTTGTTTCTACTACATACTAACGCCAATGCGGT
AGGGAAATCGGCGGGTGGAGTCTCGATCCGTCGAGCCTGTTCAACAGCGCTTGCACGCAACGATTTGTCGGATCGAAGGCTGTCGTAGAACAGATGCTCG
TGGGTCTCGCCGGCAGCGAGGATCAGGCCCGGAAGATCCCCGGTGCCATACAACTTGTTCATGTGCCCGCGACGCGGGCCAAGTTCAAGGCGGTAGAGCG
GTACGTGCGTCCGGCCAGCCGGCAGATCGCATTCTAGCTGGAATGTCGCCCTAGCCAGCGAACCTGGATAGACGGTTATCCTGAACTGCACGCCACGAGG
AACGCCCCCTGCCACATCAACTCGGCACTCTAGGCAGCGCCGCTGCTCGTTCATATTCTTGGATTCAACGCTGCGCCATCTGAATGGTCGCAGCAGTTGC
TTCTCTTCTCGAAAGATCCGGTCGGCTATCTCAAACGCAATCGGCATCCGCCGATGGAACCACAACCGAGGATTTTACGCGAATCCAAACCGATGGGCGC
AACCTATAGCGTCGACAGAAACCCCGTCAGAGGTGACGGGCCTCCCCTTGCGTTTGATAGGCCGAATCTATCGCAGTCAGGACCAGTCCTGATCGTAAGG
AAGACCAAGGCGGATCGCCAATGCCTGCTTCGAAACGCCAAGAGCTTTCGAAAGCGTCCCTAGTTCCGTTTTCCCGTCATCCTGAAGACGATTCACGAGC
GGCCAAGGCATCAAAATCTCGGCGGCGTAGCGGTTCGCTTCCCGCTCAAGTTCGTTTGAGAGGCAACGGTAAAATTCGTCGTCCTCAATGCCGCTCGCTG
CTTTCCCTCGATGCAGCACAAAGTGCGCAATCTCATGAGCAACCGTGAACCTCTTACGGGCTGGCGGATGGGCAGAATTAACGATGATCGCATAGCCAGA
GTCCCCGCCGATCTCCTCGTCACGGCGAAGGACGCCGGAGACATGTCGACCGAGATCATCTTGGTAGACATTGATGCCCAGATCACGCGCAAGCGCGCCT
ACGTCAACGGGGGCGGCGCTTTGGTGCCGCCTTATTACCTCCAGCGCGCTGTCTGCTGTCTCCCCCGGGAACCTGGGGGAAGTCGGCATCCGGGCCAGGG
CCGCCACCATCATCGTCTCCCTCATCTACTTTCTTTGTCGTTCGGCGAGCCGCATCCTCACGCCTAAGATCATCCAACTGAGCGCGGACCATCTCTTTGA
ACCCATCATGCTCAAGAGCCTTTGCCACTGCCTCGGGAACGGCTTCATCCGCCTTCTTCAAGGCTAAACTCTGAATCGTATTGTATCCCCACACCCCGAG
AATGGCTATTCCGATACCCAGCGCCGTGATCACGACGACTGCCGTCGTTAGCAATATGGTGATCAGATCGATGAACTTCCAACCTTCCGGCGCGTCATCA
ATCACGTGAGAGCCTACAAACACCAAACCCAAGCCGATGATGACTAGGGCGGTTATGTAGACGATGAACTCAAGCGGCTTCATGCGGGGAGTGTGCAGCA
ATCGCCTTGGGAGCGTCAATTTCGTGAGATGCGGGCTCGCCTCGATCTCATAAACCTGGGTTCAAGCGAGTGCACCCCGGTGCACCGATCAGCCTGATAT
GGCTTGCATGCGTTGGTGCCAAGGCGATACGCCGCACCACCGACAATGCCAGCATTTAGACAGGTGAAATGCTACCATTGAATCCGACACCGGATCGGAC
TCGGCTCTCATTGATCTCATGAGACGACCTTGGTGCGACGCAAGTCTGCGGCGAGGCGCTACGCGCTACTTGCTCTCAGGGGATAATCTCC

Protein section

ORF number : 5

ORF 1

Length		Begin	End	Strand	Fusion ORF
876 bp	276 aa	66	941	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSEKFTVRDFFKRFPDDDACLQHVMEVRFGARHVCAACGVESTFHRIAKRKAYACAACGAHLYPCAGTIFQDSRTSLQTWFYVIFLFVTTRHGVSGKEIE
RTVGVTYKTAWRMGQQIRQLMEKADGFDLMRGHVELDEAYVGGHRPGKRGRGAAGKTIVMGMKERGGRMSAEVIPNIKKATLREVTLRNVEPGSIVSTDE
LMSYGLLDGDGFKHGTVKHGAKEFAYYDYRHDATHHTNNVESFWWLFKRSIASTHIHVSQKYMNRYLAEFTFRSNY

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
759 bp	252 aa	1059	1817	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MNITTSTQVLIERVREAVVRLAGAREMDGRVVVDLPIMYPSGAMVVVQVERNGDKVLVSDMGYGLVEAELSAAEEFYGSSAAKAADEFSVGFDGYAIFAL
WVPIGRLESAVACVGNASSRAAAEAVRRAADVQSRSQNERIFDRIAKVFGKKIVTRSAEISGRHVAWPAHNVVVFPNRRRAIFEYMTNHANSASNKFMMF
SDIRAVDESISLNTVVKSVDELGEKAQMVGDVANIVPMQATDDQFRRFAEAS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
534 bp	177 aa	1814	2347	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MPIAFEIADRIFREEKQLLRPFRWRSVESKNMNEQRRCLECRVDVAGGVPRGVQFRITVYPGSLARATFQLECDLPAGRTHVPLYRLELGPRRGHMNKLY
GTGDLPGLILAAGETHEHLFYDSLRSDKSLRASAVEQARRIETPPADFPTALALVCSRNNIINGSDIPNPGDQGRLL

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
549 bp	182 aa	2477	3025	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRETMMVAALARMPTSPRFPGETADSALEVIRRHQSAAPVDVGALARDLGINVYQDDLGRHVSGVLRRDEEIGGDSGYAIIVNSAHPPARKRFTVAHEIA
HFVLHRGKAASGIEDDEFYRCLSNELEREANRYAAEILMPWPLVNRLQDDGKTELGTLSKALGVSKQALAIRLGLPYDQDWS

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
123 bp	40 aa	3026	3148	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MKPFPRQWQRLLSMMGSKRWSALSWMILGVRMRLAERQRK

Blast result :

Comments

ISApr4 is 81% aa similar to ISSpo3.
The transposase is the first ORF, others are passenger gene.

References

1] Hagstrom,A., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K.,Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.