ISSde4
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007954 | ND | Shewanella denitrificans | Shewanella denitrificans OS217 |
DNA section
IS Length : 1379 bp
Ends
IR Length : 15/17
IRL : taatggaATGGACCCATCCACTTTTAATCGGCCTCGCAATGGGCCACGAT
IRR : ----tatATGGACCCATCCGGTTTGCAAGGCATTCGATATTAATTTTGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACAAGGA | TGCCGAGCTGGT | 0 | |
| TCATGGA | TGCCCATTGAAC | 0 | |
| TCATGGA | TGAAGAATTTGA | 0 | |
| CCATGGA | TGGCCGAGAAAG | 0 |
DNA sequence
TAATGGAATGGACCCATCCACTTTTAATCGGCCTCGCAATGGGCCACGATGTAGTTGCTAAAACTCATCAACAAAGAGGACGGGTCCACCATGAAGATTA
CTACAGTTGGTTTAGATATCGCAAAGTCTGTTTTTCATTTTGTTGGTGTAAACAAAGCGGGAAAACTAGTCAAAAAGAAGATGATAAAGCGTAAAGACTT
GCTCCACTTTCTTGCTCAGATAGAGCCCTGCCTGGTCGTCATGGAAGCCTGTGGTGGAGCAAATTACTGGGCAAGGGAATTTGAGAAAGTAGGCCATCAA
GTCAAATTAATAGCACCTCAGTATGTTGTTCCCTACAGGCAGGGAAATAAAAATGACTATAACGATGCACTTGCTATAGCGGAAGCAGCCCAAAGACCTA
ACATGCGCTTTGTACAACCTAAGCCCGCAGAGCAACAAGATATTCAGATGTTACACCGAATGAGAGAAAGGCTTAGCAAGCAGTCTACTGCACTGGTTAA
TCAGGTCAGAGGAATGCTCGCTGAGTACGGGATTGTCATCACAAAAGGAAAAGCGTCTTTTAGGTCAAAACTGCCAGATATTCTAGAAAACGCCGACAAT
GAGTTAACCGCCAAAGGTAGAAGCATTTTCTATCAACTGTACGAAGAGTTTAACGGTATCGAGGAGCGGCTTAAACGCTGTGACAATCAGGTGCAGCAAG
AGACGAAGAACAATCATGTATGTCAGCGTTTAGAAAGTGTACCAGGTATCGGTCCTGTAACAGCAACTGCATTTTATGCCGCAGTAGGAGAAGGCAAAGA
CTTTACTAATGGCAGGCATTTTTCTGCATGGTGCGGCCTAGTGCCAAAACAGCACAGCAGTGGCGGTAAAGATAATTTGCTGGGGATCAGTAAACGCGGC
AATGCTTATCTTCGAACTTTATTTATCCATGGAGCAAGAGCTGTCCTTTACCATAGTGATGGTAAAGGAGATAGATTTAGTCGCTGGGCAATAGCACTTG
CAGAAAGGCGAGGATTTAACAGAGCTTGCGTTGCAGTGGCTAACAAGCTAGCGCGAATAGCATGGGTCATTGCAGCAAGAGAGGATGAATATCGGATGGC
AGTATAAAGATCCAAAGCCGATGTTGATGTTTTAACGATAATTTTTTACCAACCACCAAGTTGCTTAGACACTTGATTTGATGATGAGACAGTCAGACTG
ATCTACTTAAAACCTTTTTTCGGCATAGGCTCTAAAGAAGCCGTAAGGATGATAAGGAAAGTAGAAGCACATATCCATCAGGGCCAGAGGTAATCTCATA
AATAGGCCGAATATATGGGTGCAATGAACTCTTCTCAAAATTAATATCGAATGCCTTGCAAACCGGATGGGTCCATATA
CTACAGTTGGTTTAGATATCGCAAAGTCTGTTTTTCATTTTGTTGGTGTAAACAAAGCGGGAAAACTAGTCAAAAAGAAGATGATAAAGCGTAAAGACTT
GCTCCACTTTCTTGCTCAGATAGAGCCCTGCCTGGTCGTCATGGAAGCCTGTGGTGGAGCAAATTACTGGGCAAGGGAATTTGAGAAAGTAGGCCATCAA
GTCAAATTAATAGCACCTCAGTATGTTGTTCCCTACAGGCAGGGAAATAAAAATGACTATAACGATGCACTTGCTATAGCGGAAGCAGCCCAAAGACCTA
ACATGCGCTTTGTACAACCTAAGCCCGCAGAGCAACAAGATATTCAGATGTTACACCGAATGAGAGAAAGGCTTAGCAAGCAGTCTACTGCACTGGTTAA
TCAGGTCAGAGGAATGCTCGCTGAGTACGGGATTGTCATCACAAAAGGAAAAGCGTCTTTTAGGTCAAAACTGCCAGATATTCTAGAAAACGCCGACAAT
GAGTTAACCGCCAAAGGTAGAAGCATTTTCTATCAACTGTACGAAGAGTTTAACGGTATCGAGGAGCGGCTTAAACGCTGTGACAATCAGGTGCAGCAAG
AGACGAAGAACAATCATGTATGTCAGCGTTTAGAAAGTGTACCAGGTATCGGTCCTGTAACAGCAACTGCATTTTATGCCGCAGTAGGAGAAGGCAAAGA
CTTTACTAATGGCAGGCATTTTTCTGCATGGTGCGGCCTAGTGCCAAAACAGCACAGCAGTGGCGGTAAAGATAATTTGCTGGGGATCAGTAAACGCGGC
AATGCTTATCTTCGAACTTTATTTATCCATGGAGCAAGAGCTGTCCTTTACCATAGTGATGGTAAAGGAGATAGATTTAGTCGCTGGGCAATAGCACTTG
CAGAAAGGCGAGGATTTAACAGAGCTTGCGTTGCAGTGGCTAACAAGCTAGCGCGAATAGCATGGGTCATTGCAGCAAGAGAGGATGAATATCGGATGGC
AGTATAAAGATCCAAAGCCGATGTTGATGTTTTAACGATAATTTTTTACCAACCACCAAGTTGCTTAGACACTTGATTTGATGATGAGACAGTCAGACTG
ATCTACTTAAAACCTTTTTTCGGCATAGGCTCTAAAGAAGCCGTAAGGATGATAAGGAAAGTAGAAGCACATATCCATCAGGGCCAGAGGTAATCTCATA
AATAGGCCGAATATATGGGTGCAATGAACTCTTCTCAAAATTAATATCGAATGCCTTGCAAACCGGATGGGTCCATATA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1017 bp | 338 aa | 88 | 1104 | + | No |
Chemistry : DEDD
ORF sequence :
MKITTVGLDIAKSVFHFVGVNKAGKLVKKKMIKRKDLLHFLAQIEPCLVVMEACGGANYWAREFEKVGHQVKLIAPQYVVPYRQGNKNDYNDALAIAEAA
QRPNMRFVQPKPAEQQDIQMLHRMRERLSKQSTALVNQVRGMLAEYGIVITKGKASFRSKLPDILENADNELTAKGRSIFYQLYEEFNGIEERLKRCDNQ
VQQETKNNHVCQRLESVPGIGPVTATAFYAAVGEGKDFTNGRHFSAWCGLVPKQHSSGGKDNLLGISKRGNAYLRTLFIHGARAVLYHSDGKGDRFSRWA
IALAERRGFNRACVAVANKLARIAWVIAAREDEYRMAV
QRPNMRFVQPKPAEQQDIQMLHRMRERLSKQSTALVNQVRGMLAEYGIVITKGKASFRSKLPDILENADNELTAKGRSIFYQLYEEFNGIEERLKRCDNQ
VQQETKNNHVCQRLESVPGIGPVTATAFYAAVGEGKDFTNGRHFSAWCGLVPKQHSSGGKDNLLGISKRGNAYLRTLFIHGARAVLYHSDGKGDRFSRWA
IALAERRGFNRACVAVANKLARIAWVIAAREDEYRMAV
Blast result :
Comments
The inverted repeats are not at the termini of the IS but start at position 8 on the left end and at position 4 on the right end. The ISSde4 transposase as 80% identity to ISSod19. There are 4 copies of ISSde4 in the Shewanella denitrificans genome.
References
1] Romine, M. F. (2008) Direct submission.