ISBce15
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003909 | ND | Bacillus cereus | Bacillus cereus ATCC 10987 |
DNA section
IS Length : 1447 bp
Ends
IR Length : 22/29
IRL : TGAACCGCACCCAATTTGTTAGACAAATAACTAACAATTGGAGGTGCGGT
IRR : TGAACTGGACCCGAAAAGTTAGACATATATTTAAGCAGCTAATAAGGATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTACCTTTTCCGCG | AAG | CAGGGTGGTTCTC | 3 |
DNA sequence
TGAACCGCACCCAATTTGTTAGACAAATAACTAACAATTGGAGGTGCGGTTTTATTATGTCTAGAAAATATAAATTCGAATTAAAAAGAAATGCCGTTGA
ACATTATTTAAATACAAATGATTCTTTTAAAGTGACTGCCAAAAAATATCAAGTCAGTATATCGTTATTAAAAGAGTGGGTGGCACGTACTAGAGAACAA
GGAATAGAGTCCTTAAACTCTAAATGTACAAGGTATGACATTCAGTTTAAAATGGAAGTATTGAATTTTATGAACAATACAGGAGCTTCGCCCCTACAAG
CGGCCGCTATGTTCAACGTTCCATCTCCTCGTACCGTTAGGAAGTGGCGATCAATGCTAGAATCACAGGGGATAGATGCTCTTCGTAAAACAAGTAAGGG
GTACTCCACTGTGAAGAAGAAACAGGCTATTGCACCTAAGAAAAAACAAAATATTAACTCTCAAAATGATTTACAAAAAGAAATTGAATACTTACGCATG
GAAAATGCTTATTTAAAAAAGTTACACGCCTTAATTCAACAAAAACAACAATCTCTGAAAAAGACAAAGCACACATAATATATGAGTTAAGGCATGAATA
TAAAATTATCGACTTGATTAAAGCGGCTGAAATGGCTCGTAGTACATATTATTACTGGGTGAAACAAATGAGTCGTGAAGATAAATATAACGAGGTTAAA
GAAAGTATTAAGCAGATTTTTCAGGAACACCAAGGGCGATATGGCTATAGGCGTATCACACTTGAATTACGTAATCAGGGCTATACCATTAACCACAAAA
CCGTCCTTCGCTTAATGAATCAATTAGGGTTAAAATCGTTGGTAAGAATCACTAAATACCGCTCTTATCGCGGGAAGGTTGGGAAGGTAGCGCCAAATGT
CTTGAAGCGTGATTTCAAGGCCTCTAAGCCTAATGAAAAGTGGGTTACAGATGTTACTGAAGTTCATTTGTTTGGTGAAAAACTCTACCTATCTCCTATT
CTAGACTTGTTTAATGGTGAGATTATTTCCTATAATATTGAGAAACGCCCTACATTCCTTCTAGTCGAAAGGATGTTAGATAAGGCTTTAAAATGCTTGG
ATAAAGAAGATACTCCTATCCTTCATTCAGATCAAGGTTGGCACTATCAAATGGATAGATATCAATATATTTTAAGAGAAAATAATATAACTCAAAGTAT
GTCCCGTAAGGGAAATTGCTTAGATAATGCAGTCATTGAAAATTTCTTTGGCTTATTAAAATCTGAATTACTTTATCTTAAAGATTTTGAAAGTATGGAA
CACTTTAGGGAAGAACTAGAGAATTATATTTATTATTACAATAATAAAAGGATTAAGGCAAAATTAAAAGGATTGCCTCCTGTAAAATACAGAATTCAAT
CCTTATTAGCTGCTTAAATATATGTCTAACTTTTCGGGTCCAGTTCA
ACATTATTTAAATACAAATGATTCTTTTAAAGTGACTGCCAAAAAATATCAAGTCAGTATATCGTTATTAAAAGAGTGGGTGGCACGTACTAGAGAACAA
GGAATAGAGTCCTTAAACTCTAAATGTACAAGGTATGACATTCAGTTTAAAATGGAAGTATTGAATTTTATGAACAATACAGGAGCTTCGCCCCTACAAG
CGGCCGCTATGTTCAACGTTCCATCTCCTCGTACCGTTAGGAAGTGGCGATCAATGCTAGAATCACAGGGGATAGATGCTCTTCGTAAAACAAGTAAGGG
GTACTCCACTGTGAAGAAGAAACAGGCTATTGCACCTAAGAAAAAACAAAATATTAACTCTCAAAATGATTTACAAAAAGAAATTGAATACTTACGCATG
GAAAATGCTTATTTAAAAAAGTTACACGCCTTAATTCAACAAAAACAACAATCTCTGAAAAAGACAAAGCACACATAATATATGAGTTAAGGCATGAATA
TAAAATTATCGACTTGATTAAAGCGGCTGAAATGGCTCGTAGTACATATTATTACTGGGTGAAACAAATGAGTCGTGAAGATAAATATAACGAGGTTAAA
GAAAGTATTAAGCAGATTTTTCAGGAACACCAAGGGCGATATGGCTATAGGCGTATCACACTTGAATTACGTAATCAGGGCTATACCATTAACCACAAAA
CCGTCCTTCGCTTAATGAATCAATTAGGGTTAAAATCGTTGGTAAGAATCACTAAATACCGCTCTTATCGCGGGAAGGTTGGGAAGGTAGCGCCAAATGT
CTTGAAGCGTGATTTCAAGGCCTCTAAGCCTAATGAAAAGTGGGTTACAGATGTTACTGAAGTTCATTTGTTTGGTGAAAAACTCTACCTATCTCCTATT
CTAGACTTGTTTAATGGTGAGATTATTTCCTATAATATTGAGAAACGCCCTACATTCCTTCTAGTCGAAAGGATGTTAGATAAGGCTTTAAAATGCTTGG
ATAAAGAAGATACTCCTATCCTTCATTCAGATCAAGGTTGGCACTATCAAATGGATAGATATCAATATATTTTAAGAGAAAATAATATAACTCAAAGTAT
GTCCCGTAAGGGAAATTGCTTAGATAATGCAGTCATTGAAAATTTCTTTGGCTTATTAAAATCTGAATTACTTTATCTTAAAGATTTTGAAAGTATGGAA
CACTTTAGGGAAGAACTAGAGAATTATATTTATTATTACAATAATAAAAGGATTAAGGCAAAATTAAAAGGATTGCCTCCTGTAAAATACAGAATTCAAT
CCTTATTAGCTGCTTAAATATATGTCTAACTTTTCGGGTCCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 522 bp | 173 aa | 57 | 578 | + | No |
Description : First part of the transposase
ORF sequence :
MSRKYKFELKRNAVEHYLNTNDSFKVTAKKYQVSISLLKEWVARTREQGIESLNSKCTRYDIQFKMEVLNFMNNTGASPLQAAAMFNVPSPRTVRKWRSM
LESQGIDALRKTSKGYSTVKKKQAIAPKKKQNINSQNDLQKEIEYLRMENAYLKKLHALIQQKQQSLKKTKHT
LESQGIDALRKTSKGYSTVKKKQAIAPKKKQNINSQNDLQKEIEYLRMENAYLKKLHALIQQKQQSLKKTKHT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 930 bp | 309 aa | 488 | 1417 | + | No |
Description : Second part of the transposase
ORF sequence :
ILTHGKCLFKKVTRLNSTKTTISEKDKAHIIYELRHEYKIIDLIKAAEMARSTYYYWVKQMSREDKYNEVKESIKQIFQEHQGRYGYRRITLELRNQGYT
INHKTVLRLMNQLGLKSLVRITKYRSYRGKVGKVAPNVLKRDFKASKPNEKWVTDVTEVHLFGEKLYLSPILDLFNGEIISYNIEKRPTFLLVERMLDKA
LKCLDKEDTPILHSDQGWHYQMDRYQYILRENNITQSMSRKGNCLDNAVIENFFGLLKSELLYLKDFESMEHFREELENYIYYYNNKRIKAKLKGLPPVK
YRIQSLLAA
INHKTVLRLMNQLGLKSLVRITKYRSYRGKVGKVAPNVLKRDFKASKPNEKWVTDVTEVHLFGEKLYLSPILDLFNGEIISYNIEKRPTFLLVERMLDKA
LKCLDKEDTPILHSDQGWHYQMDRYQYILRENNITQSMSRKGNCLDNAVIENFFGLLKSELLYLKDFESMEHFREELENYIYYYNNKRIKAKLKGLPPVK
YRIQSLLAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1358 bp | 453 aa | 57 | 1414 | + | Yes |
Chemistry : DDE
ORF sequence :
MSRKYKFELKRNAVEHYLNTNDSFKVTAKKYQVSISLLKEWVARTREQGIESLNSKCTRYDIQFKMEVLNFMNNTGASPLQAAAMFNVPSPRTVRKWRSM
LESQGIDALRKTSKGYSTVKKKQAIAPKKKQNINSQNDLQKEIEYLRMENAYLKKVTRLNSTKTTISEKDKAHIIYELRHEYKIIDLIKAAEMARSTYYY
WVKQMSREDKYNEVKESIKQIFQEHQGRYGYRRITLELRNQGYTINHKTVLRLMNQLGLKSLVRITKYRSYRGKVGKVAPNVLKRDFKASKPNEKWVTDV
TEVHLFGEKLYLSPILDLFNGEIISYNIEKRPTFLLVERMLDKALKCLDKEDTPILHSDQGWHYQMDRYQYILRENNITQSMSRKGNCLDNAVIENFFGL
LKSELLYLKDFESMEHFREELENYIYYYNNKRIKAKLKGLPPVKYRIQSLLAA
LESQGIDALRKTSKGYSTVKKKQAIAPKKKQNINSQNDLQKEIEYLRMENAYLKKVTRLNSTKTTISEKDKAHIIYELRHEYKIIDLIKAAEMARSTYYY
WVKQMSREDKYNEVKESIKQIFQEHQGRYGYRRITLELRNQGYTINHKTVLRLMNQLGLKSLVRITKYRSYRGKVGKVAPNVLKRDFKASKPNEKWVTDV
TEVHLFGEKLYLSPILDLFNGEIISYNIEKRPTFLLVERMLDKALKCLDKEDTPILHSDQGWHYQMDRYQYILRENNITQSMSRKGNCLDNAVIENFFGL
LKSELLYLKDFESMEHFREELENYIYYYNNKRIKAKLKGLPPVKYRIQSLLAA
Blast result :
Comments
ISBce15 is 66% aa similar to ISEnfa3.The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Rasko,D.A., Ravel,J., Okstad,O.A., Helgason,E., Cer,R.Z., Jiang,L., Shores,K.A., Fouts,D.E., Tourasse,N.J., Angiuoli,S.V., Kolonay,J., Nelson,W.C., Kolsto,A.-B., Fraser,C.M. and Read,T.D. (2004) Nucleic Acids Res. 32 (3), 977-988.
2] ISfinder annotation (2008).
2] ISfinder annotation (2008).