ISBope1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010170 | ND | Bordetella petrii | Bordetella petrii |
DNA section
IS Length : 1458 bp
Ends
IR Length : 34/40
IRL : TGTCAGGCACCCTGCGGTGAAGTGACCCCCAAAAGTTGGATACCAACTTA
IRR : TGTCAGGCTCCCGCAGGTGAAGTGACCCCACAAAGTTGGACAGTTTTGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCAGTCTCTGCCTGCTTCGG | CCTGTGGGCGGCGACGCGCCT | 0 |
DNA sequence
TGTCAGGCACCCTGCGGTGAAGTGACCCCCAAAAGTTGGATACCAACTTATGGGGGTATTTTTATGGTGAAGTACAGCGAACAGACCAAATTTGCAGCGG
TCGAGGAGTATTGCCGGGGCGATTTGGGACTGGAAGCCGTGGCGCAACGCCACGGCGTGAACCCGCATGCGCTGCGACGCTGGGCGGCGGCCTACCGGGA
GCATGGCGCCTCGGGCGTGCGCGCCAAGCGCGGCCGCTACAGCGCCTCGTTCAAGCAGACGGTGCTGCAACAGATGAAACAGCAGGGCCTGTCGTATCGG
CAGGCGGCAGCGCGGTTCGACATCCGCAACTTCAACATCATCGCCCGCTGGGCACGCCAGTATGATGTACAGGGTCTAGCCGCTTTATCTACAGGACAGC
CCCCCGCAATGACCTCGCCCGTGAAACCGGCATCGCCCGATGCCACGCGTACACGCGAAGATCTGCTCAAGGAACTGCAGTTTCTGCGCATGGAGAACGC
GTACCTAAAAAAGCTCGATGCCTTGGTTCAAGCCAAACAGAAATCGGCGCAGCAGAAAAAGCGCAAATAGTGCTTGAACTAAGGCAGCAATTCCCTCTTG
CGGGCTTGTTGTCGGTGGTGGGGCTGTCACGCAGCACCTTCTACTACCAATGGCGGGCCGGGCAAGCCGAAGACAAGTTCGCGTCATTGAAGTCCCGGAT
CTGCGCAATTTTTGCCCAGCATCACGGCCGCTACGGCTATCGACGCATCACCGAGGCGATCCGCCAGCAAGGCTGGCCGGTCAACCACAAGACGGTGCAG
CGCCTGATGGGCCTGTTGGGGTTGAAGTCTTGCGTACGGGTCAAGAAGTATCGCTCCTACCGAGGCACGCTGAGCCAGCTCGCCCCGAACGTGCTGCGAC
GCCAGTTCAGGGCTGCTCGCCCGAATCAGAAGTGGGCCACCGATGTGACCGAGTTCAGGGTCGGCACCCAGAAGCTGTACCTCTCGCCGGTGATGGACCT
GTGCAATGGCGAGATCATCGCCTACGAAATGGCCTGCCGTCCCAGCTTCGCCATGGTCGCAGCCATGCTCAGGCAAGCCTGCGCCCGGCTCAGCCCGTAC
GAGCGGCCCATCCTGCACTCGGACCAGGGGTGGCAATACCAGATGCCCGCCTACCGCGAGCTCCTGGCGCAGCACGCGGTCACCCAGAGCATGTCACGAC
AGGGAAATTGCCTGGACAATGCCGCCATCGAGAGCTTCTTCGGCACCCTGAAGGCCGAGTTCTTCCACCTGAACACCTTCCAGACCATCGAACAGCTCAA
GGACGGGATCGACCGATATATCCACTACTACAATCATCACCGCATTAAGCTCAAACTTAACGGGTTGAGTCCGGTGCAATACCGGACTCAGCTCCTGGCC
ACATAACGGCCAAAACTGTCCAACTTTGTGGGGTCACTTCACCTGCGGGAGCCTGACA
TCGAGGAGTATTGCCGGGGCGATTTGGGACTGGAAGCCGTGGCGCAACGCCACGGCGTGAACCCGCATGCGCTGCGACGCTGGGCGGCGGCCTACCGGGA
GCATGGCGCCTCGGGCGTGCGCGCCAAGCGCGGCCGCTACAGCGCCTCGTTCAAGCAGACGGTGCTGCAACAGATGAAACAGCAGGGCCTGTCGTATCGG
CAGGCGGCAGCGCGGTTCGACATCCGCAACTTCAACATCATCGCCCGCTGGGCACGCCAGTATGATGTACAGGGTCTAGCCGCTTTATCTACAGGACAGC
CCCCCGCAATGACCTCGCCCGTGAAACCGGCATCGCCCGATGCCACGCGTACACGCGAAGATCTGCTCAAGGAACTGCAGTTTCTGCGCATGGAGAACGC
GTACCTAAAAAAGCTCGATGCCTTGGTTCAAGCCAAACAGAAATCGGCGCAGCAGAAAAAGCGCAAATAGTGCTTGAACTAAGGCAGCAATTCCCTCTTG
CGGGCTTGTTGTCGGTGGTGGGGCTGTCACGCAGCACCTTCTACTACCAATGGCGGGCCGGGCAAGCCGAAGACAAGTTCGCGTCATTGAAGTCCCGGAT
CTGCGCAATTTTTGCCCAGCATCACGGCCGCTACGGCTATCGACGCATCACCGAGGCGATCCGCCAGCAAGGCTGGCCGGTCAACCACAAGACGGTGCAG
CGCCTGATGGGCCTGTTGGGGTTGAAGTCTTGCGTACGGGTCAAGAAGTATCGCTCCTACCGAGGCACGCTGAGCCAGCTCGCCCCGAACGTGCTGCGAC
GCCAGTTCAGGGCTGCTCGCCCGAATCAGAAGTGGGCCACCGATGTGACCGAGTTCAGGGTCGGCACCCAGAAGCTGTACCTCTCGCCGGTGATGGACCT
GTGCAATGGCGAGATCATCGCCTACGAAATGGCCTGCCGTCCCAGCTTCGCCATGGTCGCAGCCATGCTCAGGCAAGCCTGCGCCCGGCTCAGCCCGTAC
GAGCGGCCCATCCTGCACTCGGACCAGGGGTGGCAATACCAGATGCCCGCCTACCGCGAGCTCCTGGCGCAGCACGCGGTCACCCAGAGCATGTCACGAC
AGGGAAATTGCCTGGACAATGCCGCCATCGAGAGCTTCTTCGGCACCCTGAAGGCCGAGTTCTTCCACCTGAACACCTTCCAGACCATCGAACAGCTCAA
GGACGGGATCGACCGATATATCCACTACTACAATCATCACCGCATTAAGCTCAAACTTAACGGGTTGAGTCCGGTGCAATACCGGACTCAGCTCCTGGCC
ACATAACGGCCAAAACTGTCCAACTTTGTGGGGTCACTTCACCTGCGGGAGCCTGACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 507 bp | 168 aa | 64 | 570 | + | No |
Description : First part of the transposase
ORF sequence :
MVKYSEQTKFAAVEEYCRGDLGLEAVAQRHGVNPHALRRWAAAYREHGASGVRAKRGRYSASFKQTVLQQMKQQGLSYRQAAARFDIRNFNIIARWARQY
DVQGLAALSTGQPPAMTSPVKPASPDATRTREDLLKELQFLRMENAYLKKLDALVQAKQKSAQQKKRK
DVQGLAALSTGQPPAMTSPVKPASPDATRTREDLLKELQFLRMENAYLKKLDALVQAKQKSAQQKKRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1041 bp | 346 aa | 366 | 1406 | + | No |
Description : Second part of the transposase
ORF sequence :
CTGSSRFIYRTAPRNDLARETGIARCHAYTRRSAQGTAVSAHGERVPKKARCLGSSQTEIGAAEKAQIVLELRQQFPLAGLLSVVGLSRSTFYYQWRAGQ
AEDKFASLKSRICAIFAQHHGRYGYRRITEAIRQQGWPVNHKTVQRLMGLLGLKSCVRVKKYRSYRGTLSQLAPNVLRRQFRAARPNQKWATDVTEFRVG
TQKLYLSPVMDLCNGEIIAYEMACRPSFAMVAAMLRQACARLSPYERPILHSDQGWQYQMPAYRELLAQHAVTQSMSRQGNCLDNAAIESFFGTLKAEFF
HLNTFQTIEQLKDGIDRYIHYYNHHRIKLKLNGLSPVQYRTQLLAT
AEDKFASLKSRICAIFAQHHGRYGYRRITEAIRQQGWPVNHKTVQRLMGLLGLKSCVRVKKYRSYRGTLSQLAPNVLRRQFRAARPNQKWATDVTEFRVG
TQKLYLSPVMDLCNGEIIAYEMACRPSFAMVAAMLRQACARLSPYERPILHSDQGWQYQMPAYRELLAQHAVTQSMSRQGNCLDNAAIESFFGTLKAEFF
HLNTFQTIEQLKDGIDRYIHYYNHHRIKLKLNGLSPVQYRTQLLAT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1343 bp | 447 aa | 64 | 1406 | + | Yes |
Chemistry : DDE
ORF sequence :
MVKYSEQTKFAAVEEYCRGDLGLEAVAQRHGVNPHALRRWAAAYREHGASGVRAKRGRYSASFKQTVLQQMKQQGLSYRQAAARFDIRNFNIIARWARQY
DVQGLAALSTGQPPAMTSPVKPASPDATRTREDLLKELQFLRMENAYLKKARCLGSSQTEIGAAEKAQIVLELRQQFPLAGLLSVVGLSRSTFYYQWRAG
QAEDKFASLKSRICAIFAQHHGRYGYRRITEAIRQQGWPVNHKTVQRLMGLLGLKSCVRVKKYRSYRGTLSQLAPNVLRRQFRAARPNQKWATDVTEFRV
GTQKLYLSPVMDLCNGEIIAYEMACRPSFAMVAAMLRQACARLSPYERPILHSDQGWQYQMPAYRELLAQHAVTQSMSRQGNCLDNAAIESFFGTLKAEF
FHLNTFQTIEQLKDGIDRYIHYYNHHRIKLKLNGLSPVQYRTQLLAT
DVQGLAALSTGQPPAMTSPVKPASPDATRTREDLLKELQFLRMENAYLKKARCLGSSQTEIGAAEKAQIVLELRQQFPLAGLLSVVGLSRSTFYYQWRAG
QAEDKFASLKSRICAIFAQHHGRYGYRRITEAIRQQGWPVNHKTVQRLMGLLGLKSCVRVKKYRSYRGTLSQLAPNVLRRQFRAARPNQKWATDVTEFRV
GTQKLYLSPVMDLCNGEIIAYEMACRPSFAMVAAMLRQACARLSPYERPILHSDQGWQYQMPAYRELLAQHAVTQSMSRQGNCLDNAAIESFFGTLKAEF
FHLNTFQTIEQLKDGIDRYIHYYNHHRIKLKLNGLSPVQYRTQLLAT
Blast result :
Comments
ISBope1 is 76% aa similar to ISBam1.The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] NCBI Genome Project (2007) Direct Submission GenBank.
2] ISfinder annotation (2008).
2] ISfinder annotation (2008).