ISApl3
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009053 | ND | Actinobacillus pleuropneumoniae | Actinobacillus pleuropneumoniae L20 |
DNA section
IS Length : 1418 bp
Ends
IR Length : 22/25
IRL : TGATCTGCCCCCCAAAAGTTGGACTAAACCTCCAACGGATTAAGGTGCAG
IRR : TGATCTGACCCCAAAAAGTTAGACTGTTTAATTTAAGGACTGAGTTCTGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATCACAAGCCCTTTGTTA | CTA | ACAAAGGGCTTTTTTTA | 3 |
DNA sequence
TGATCTGCCCCCCAAAAGTTGGACTAAACCTCCAACGGATTAAGGTGCAGATTTTTTATGACTAAATATAACTCGCTATTCAAACAACAAGTCATCGAGT
TTTATCTTCAAAATGACAAAAACCGTTTATTCACTCAACGACATTTTCAATTATCCAAGAAAACCTTAACTCGATGGATTGCTCAATTTAATCATAACGG
AATCAATGGGCTAGCGGTGATAGGTAAGAAGCAAAAATATTCCCCCGAATTCAAATTAACCGTCATACAAGCGGTCAAAAAGGGGCAATTTTCTGCGGAA
AGCGCCTCCCTTCATTTTGGCATTGCCAATTCAGGTTCGATTAGCCAATGGTTGCACACCTTTGAAGAACAAGGTATAAACGGCTTATTACCCAAGCCCA
AAGGTCGTCCGACAATGAAACCGAAATACCCGAAAATGCCTCCTCCACCTAAAACTGAAGAAGAACGCTTACGTTATCGTATTTTAGAGCTGGAAGCGGA
GAATGCTTACCTAAAAAAGTTGCGGGAGTTCAACCAACAAAAAATGCGGCAAAAGCAGCCATCGTAAAAACGTTGCGAGTGGATTTTCCCTTAGAAATCC
TCCTCCCTTTGACTGGTTTAAAACGTAGTACGTTCTTTTATCACTTACCGCCTAAAATCGATAAAAATGCAGTGATTAGGCAGAAAGTGATTGAGATTTA
TCAAGATAATGATAGCAATTACGGCTATCGTCGTATTACGTTAAAGTTACGTGATTTTTTGGGTGCGATTAACCGTAAGCGTATTCAAAGGATAATGCAA
CAGCTTGATTTAAAAGGGAAATGTAAGCAGAGGAAATATCGTTCTTATCAAGGTGAAGCGGGTAAAATTGCCGAGAATGTATTGCAACGGGATTTTCACG
CAACGGCGCCAAATGAAAAGCTCGTCACGGATATCACCGAGTTTAAATGTGCGGAAGGCAAGCTCTATTTATCGCCTATTAAGGACTTGTTTAATAGTGA
AATTCTTGCTTATGATTTAGCAAGAAGCCCTAACTTTGAGCAAGTGATGCGGATGCTAAAGCAAGCGGTCGCAAAACTGCCTAAGGACGCTAAACCGATT
TTACATTCCGACCAAGGCTGGCAATACCAGATGGCAGACTATCAAGACATGCTGAGAAAACATCATATTAAACAAAGTATGTCGAGAAAAGCGAATTGCT
TGGATAATGGGGCAATGGAAAGTTTTTTTGGGCGATTGAAAACGGAATGTTACTTTGGTAAGCGATTTGAAACTTTCGAACAACTTGAGAAAGTGATTCA
CGAGTACATTCATTATTACAACAATGAGCGTATTCAAGTGAAATTAAAAGGACTCAGCCCTGTACAATACAGAACTCAGTCCTTAAATTAAACAGTCTAA
CTTTTTGGGGTCAGATCA
TTTATCTTCAAAATGACAAAAACCGTTTATTCACTCAACGACATTTTCAATTATCCAAGAAAACCTTAACTCGATGGATTGCTCAATTTAATCATAACGG
AATCAATGGGCTAGCGGTGATAGGTAAGAAGCAAAAATATTCCCCCGAATTCAAATTAACCGTCATACAAGCGGTCAAAAAGGGGCAATTTTCTGCGGAA
AGCGCCTCCCTTCATTTTGGCATTGCCAATTCAGGTTCGATTAGCCAATGGTTGCACACCTTTGAAGAACAAGGTATAAACGGCTTATTACCCAAGCCCA
AAGGTCGTCCGACAATGAAACCGAAATACCCGAAAATGCCTCCTCCACCTAAAACTGAAGAAGAACGCTTACGTTATCGTATTTTAGAGCTGGAAGCGGA
GAATGCTTACCTAAAAAAGTTGCGGGAGTTCAACCAACAAAAAATGCGGCAAAAGCAGCCATCGTAAAAACGTTGCGAGTGGATTTTCCCTTAGAAATCC
TCCTCCCTTTGACTGGTTTAAAACGTAGTACGTTCTTTTATCACTTACCGCCTAAAATCGATAAAAATGCAGTGATTAGGCAGAAAGTGATTGAGATTTA
TCAAGATAATGATAGCAATTACGGCTATCGTCGTATTACGTTAAAGTTACGTGATTTTTTGGGTGCGATTAACCGTAAGCGTATTCAAAGGATAATGCAA
CAGCTTGATTTAAAAGGGAAATGTAAGCAGAGGAAATATCGTTCTTATCAAGGTGAAGCGGGTAAAATTGCCGAGAATGTATTGCAACGGGATTTTCACG
CAACGGCGCCAAATGAAAAGCTCGTCACGGATATCACCGAGTTTAAATGTGCGGAAGGCAAGCTCTATTTATCGCCTATTAAGGACTTGTTTAATAGTGA
AATTCTTGCTTATGATTTAGCAAGAAGCCCTAACTTTGAGCAAGTGATGCGGATGCTAAAGCAAGCGGTCGCAAAACTGCCTAAGGACGCTAAACCGATT
TTACATTCCGACCAAGGCTGGCAATACCAGATGGCAGACTATCAAGACATGCTGAGAAAACATCATATTAAACAAAGTATGTCGAGAAAAGCGAATTGCT
TGGATAATGGGGCAATGGAAAGTTTTTTTGGGCGATTGAAAACGGAATGTTACTTTGGTAAGCGATTTGAAACTTTCGAACAACTTGAGAAAGTGATTCA
CGAGTACATTCATTATTACAACAATGAGCGTATTCAAGTGAAATTAAAAGGACTCAGCCCTGTACAATACAGAACTCAGTCCTTAAATTAAACAGTCTAA
CTTTTTGGGGTCAGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 510 bp | 169 aa | 58 | 567 | + | No |
Description : First part of the transposase
ORF sequence :
MTKYNSLFKQQVIEFYLQNDKNRLFTQRHFQLSKKTLTRWIAQFNHNGINGLAVIGKKQKYSPEFKLTVIQAVKKGQFSAESASLHFGIANSGSISQWLH
TFEEQGINGLLPKPKGRPTMKPKYPKMPPPPKTEEERLRYRILELEAENAYLKKLREFNQQKMRQKQPS
TFEEQGINGLLPKPKGRPTMKPKYPKMPPPPKTEEERLRYRILELEAENAYLKKLREFNQQKMRQKQPS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 933 bp | 310 aa | 459 | 1391 | + | No |
Description : Second part of the transposase
ORF sequence :
RRTLTLSYFRAGSGECLPKKVAGVQPTKNAAKAAIVKTLRVDFPLEILLPLTGLKRSTFFYHLPPKIDKNAVIRQKVIEIYQDNDSNYGYRRITLKLRDF
LGAINRKRIQRIMQQLDLKGKCKQRKYRSYQGEAGKIAENVLQRDFHATAPNEKLVTDITEFKCAEGKLYLSPIKDLFNSEILAYDLARSPNFEQVMRML
KQAVAKLPKDAKPILHSDQGWQYQMADYQDMLRKHHIKQSMSRKANCLDNGAMESFFGRLKTECYFGKRFETFEQLEKVIHEYIHYYNNERIQVKLKGLS
PVQYRTQSLN
LGAINRKRIQRIMQQLDLKGKCKQRKYRSYQGEAGKIAENVLQRDFHATAPNEKLVTDITEFKCAEGKLYLSPIKDLFNSEILAYDLARSPNFEQVMRML
KQAVAKLPKDAKPILHSDQGWQYQMADYQDMLRKHHIKQSMSRKANCLDNGAMESFFGRLKTECYFGKRFETFEQLEKVIHEYIHYYNNERIQVKLKGLS
PVQYRTQSLN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1334 bp | 444 aa | 58 | 1391 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKYNSLFKQQVIEFYLQNDKNRLFTQRHFQLSKKTLTRWIAQFNHNGINGLAVIGKKQKYSPEFKLTVIQAVKKGQFSAESASLHFGIANSGSISQWLH
TFEEQGINGLLPKPKGRPTMKPKYPKMPPPPKTEEERLRYRILELEAENAYLKKVAGVQPTKNAAKAAIVKTLRVDFPLEILLPLTGLKRSTFFYHLPPK
IDKNAVIRQKVIEIYQDNDSNYGYRRITLKLRDFLGAINRKRIQRIMQQLDLKGKCKQRKYRSYQGEAGKIAENVLQRDFHATAPNEKLVTDITEFKCAE
GKLYLSPIKDLFNSEILAYDLARSPNFEQVMRMLKQAVAKLPKDAKPILHSDQGWQYQMADYQDMLRKHHIKQSMSRKANCLDNGAMESFFGRLKTECYF
GKRFETFEQLEKVIHEYIHYYNNERIQVKLKGLSPVQYRTQSLN
TFEEQGINGLLPKPKGRPTMKPKYPKMPPPPKTEEERLRYRILELEAENAYLKKVAGVQPTKNAAKAAIVKTLRVDFPLEILLPLTGLKRSTFFYHLPPK
IDKNAVIRQKVIEIYQDNDSNYGYRRITLKLRDFLGAINRKRIQRIMQQLDLKGKCKQRKYRSYQGEAGKIAENVLQRDFHATAPNEKLVTDITEFKCAE
GKLYLSPIKDLFNSEILAYDLARSPNFEQVMRMLKQAVAKLPKDAKPILHSDQGWQYQMADYQDMLRKHHIKQSMSRKANCLDNGAMESFFGRLKTECYF
GKRFETFEQLEKVIHEYIHYYNNERIQVKLKGLSPVQYRTQSLN
Blast result :
Comments
ISApl3 is 97% aa similar to ISApl2.
References
1] Foote,S.J., Bosse,J.T., Bouevitch,A.B., Langford,P.R., Young,N.M. and Nash,J.H.E. (2007) Direct submission GenBank.
2] ISfinder annotation (2008).
2] ISfinder annotation (2008).