ISBce14
- Family IS3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007103 | ND | Bacillus cereus | Bacillus cereus E33L |
DNA section
IS Length : 1457 bp
Ends
IR Length : 27/33
IRL : TGTACCCTATAGAATAGACACTTGAAAAAAGTCTATTCTATAGGGTATTT
IRR : TGTACCCTTTGTAAAGGACATTTTGAAAAAAGTTAGGCAACATCAAGAAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATTTTAAAA | GATTATTTCC | 0 |
DNA sequence
TGTACCCTATAGAATAGACACTTGAAAAAAGTCTATTCTATAGGGTATTTCTTGTATAATAAAAGGAAAATTGGGATCGGAGAATGTTGAAAATGACAAA
AAAACTATTTACAGAAAGAGAAATTCAAATTCTATCAAATAACTTATACGTAAAATCTGTAAGTCAGAAAGGTATCACTTATACAGAGGAATTTAAGCAT
ATTTTTATTGAGGAAAATGAAAAAGGAAAGCTACCTCGAAATATTTTTGAAGAATGTGGTTTTGATATAGATATGATTGGAATGAAACGGGTTATGTCAT
CGGGAAGTAGATGGCGTGCTGCTTATAGAAAAAATGGTGTATTGGGTTTAAGGGATACACGTATCGAAAACGCCGGAAGAACTCTTGAGAGAGAGCTTAC
GTTAGAAGAAAAGTATGCCCGTTTAGAAGCCGAACGAAACTTACTAAAGGCGGAAAACGAATTGCTAAAAAAATCAAACTTATGGAAGGGAGGATGAGAA
GGAAATAACACTACCATCTAGTCAGAAATTCATCTTGATTCGTTCTGTCATCATGAAATACAACTTAAGGAATATGGTCAGTTATTTGTGTAAAATAGCT
GGTGTATCCCGTTCGGGATACTATAATTACTTTTCAATTTCATCTCAAGAACAACGGAAACAAAAGAGTGATCGAGATGAAATCTTGAAGGAAACCATAT
TAAAAGCACTTCGATTTAGAAATAGAAAGAAGGGAGCTCGTCAGATAAAGATGACATTGGCGGGTCAGTTTCAAGTTGTCTACAATTTAAAGCGTATCCG
TAGAATTATGAAGAAATACGAGATTGTCTGTCCGGTTCGCAAGGCGAATCCTTATAAAAGAATGCTTAAAGCTACAAAAGAACATCGAATAGTACCGAAT
CAATTAAATCGAGAATTTAAACAAAATACCCCAGGGAAAACACTTCTTACAGATATCACCTATTTAGTTTACGGTAAGAATCAAAGGGCCTATTTATCTA
CAATTTTAGACGGCTCAACTAATGAAATTTTAGCTTACCATGTTTCAGAACAAATGACATTAGAGCTCGTAACGACAACTCTCCATAAACTAAAAAGGAA
TCCGCGGATTCGATTGATTGAAGGTGCTTATATTCATTCAGATCAAGGAGCCCACTACACAAGCCCTACCTATCAAAAGCTAGTCAAAAAGCTAAACCTT
GGACAATCCATGTCAAGAAGAGGAAACTGTTGGGATAACGCCCCACAAGAATCGTTTTTTGGTCATCTGAAAGATGAAGCTCATATAAAACCTTGCGCGT
CCTTTAATGAATTGAAACAAGAGATTAAGAAATATATGACGTACTATAATCACTATAGATATCAATGGAATTTAAAGAAGATGACTCCTGTTGGATACAG
AAATCATCTTCTTGATGTTGCCTAACTTTTTTCAAAATGTCCTTTACAAAGGGTACA
AAAACTATTTACAGAAAGAGAAATTCAAATTCTATCAAATAACTTATACGTAAAATCTGTAAGTCAGAAAGGTATCACTTATACAGAGGAATTTAAGCAT
ATTTTTATTGAGGAAAATGAAAAAGGAAAGCTACCTCGAAATATTTTTGAAGAATGTGGTTTTGATATAGATATGATTGGAATGAAACGGGTTATGTCAT
CGGGAAGTAGATGGCGTGCTGCTTATAGAAAAAATGGTGTATTGGGTTTAAGGGATACACGTATCGAAAACGCCGGAAGAACTCTTGAGAGAGAGCTTAC
GTTAGAAGAAAAGTATGCCCGTTTAGAAGCCGAACGAAACTTACTAAAGGCGGAAAACGAATTGCTAAAAAAATCAAACTTATGGAAGGGAGGATGAGAA
GGAAATAACACTACCATCTAGTCAGAAATTCATCTTGATTCGTTCTGTCATCATGAAATACAACTTAAGGAATATGGTCAGTTATTTGTGTAAAATAGCT
GGTGTATCCCGTTCGGGATACTATAATTACTTTTCAATTTCATCTCAAGAACAACGGAAACAAAAGAGTGATCGAGATGAAATCTTGAAGGAAACCATAT
TAAAAGCACTTCGATTTAGAAATAGAAAGAAGGGAGCTCGTCAGATAAAGATGACATTGGCGGGTCAGTTTCAAGTTGTCTACAATTTAAAGCGTATCCG
TAGAATTATGAAGAAATACGAGATTGTCTGTCCGGTTCGCAAGGCGAATCCTTATAAAAGAATGCTTAAAGCTACAAAAGAACATCGAATAGTACCGAAT
CAATTAAATCGAGAATTTAAACAAAATACCCCAGGGAAAACACTTCTTACAGATATCACCTATTTAGTTTACGGTAAGAATCAAAGGGCCTATTTATCTA
CAATTTTAGACGGCTCAACTAATGAAATTTTAGCTTACCATGTTTCAGAACAAATGACATTAGAGCTCGTAACGACAACTCTCCATAAACTAAAAAGGAA
TCCGCGGATTCGATTGATTGAAGGTGCTTATATTCATTCAGATCAAGGAGCCCACTACACAAGCCCTACCTATCAAAAGCTAGTCAAAAAGCTAAACCTT
GGACAATCCATGTCAAGAAGAGGAAACTGTTGGGATAACGCCCCACAAGAATCGTTTTTTGGTCATCTGAAAGATGAAGCTCATATAAAACCTTGCGCGT
CCTTTAATGAATTGAAACAAGAGATTAAGAAATATATGACGTACTATAATCACTATAGATATCAATGGAATTTAAAGAAGATGACTCCTGTTGGATACAG
AAATCATCTTCTTGATGTTGCCTAACTTTTTTCAAAATGTCCTTTACAAAGGGTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 414 bp | 137 aa | 84 | 497 | + | No |
Description : First part of the transposase
ORF sequence :
MLKMTKKLFTEREIQILSNNLYVKSVSQKGITYTEEFKHIFIEENEKGKLPRNIFEECGFDIDMIGMKRVMSSGSRWRAAYRKNGVLGLRDTRIENAGRT
LERELTLEEKYARLEAERNLLKAENELLKKSNLWKGG
LERELTLEEKYARLEAERNLLKAENELLKKSNLWKGG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1342 bp | 318 aa | 84 | 1425 | + | No |
Description : Second part of the transposase
ORF sequence :
KNQTYGREDEKEITLPSSQKFILIRSVIMKYNLRNMVSYLCKIAGVSRSGYYNYFSISSQEQRKQKSDRDEILKETILKALRFRNRKKGARQIKMTLAGQ
FQVVYNLKRIRRIMKKYEIVCPVRKANPYKRMLKATKEHRIVPNQLNREFKQNTPGKTLLTDITYLVYGKNQRAYLSTILDGSTNEILAYHVSEQMTLEL
VTTTLHKLKRNPRIRLIEGAYIHSDQGAHYTSPTYQKLVKKLNLGQSMSRRGNCWDNAPQESFFGHLKDEAHIKPCASFNELKQEIKKYMTYYNHYRYQW
NLKKMTPVGYRNHLLDVA
FQVVYNLKRIRRIMKKYEIVCPVRKANPYKRMLKATKEHRIVPNQLNREFKQNTPGKTLLTDITYLVYGKNQRAYLSTILDGSTNEILAYHVSEQMTLEL
VTTTLHKLKRNPRIRLIEGAYIHSDQGAHYTSPTYQKLVKKLNLGQSMSRRGNCWDNAPQESFFGHLKDEAHIKPCASFNELKQEIKKYMTYYNHYRYQW
NLKKMTPVGYRNHLLDVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1342 bp | 447 aa | 84 | 1425 | + | Yes |
Chemistry : DDE
ORF sequence :
MLKMTKKLFTEREIQILSNNLYVKSVSQKGITYTEEFKHIFIEENEKGKLPRNIFEECGFDIDMIGMKRVMSSGSRWRAAYRKNGVLGLRDTRIENAGRT
LERELTLEEKYARLEAERNLLKAENELLKKNQTYGREDEKEITLPSSQKFILIRSVIMKYNLRNMVSYLCKIAGVSRSGYYNYFSISSQEQRKQKSDRDE
ILKETILKALRFRNRKKGARQIKMTLAGQFQVVYNLKRIRRIMKKYEIVCPVRKANPYKRMLKATKEHRIVPNQLNREFKQNTPGKTLLTDITYLVYGKN
QRAYLSTILDGSTNEILAYHVSEQMTLELVTTTLHKLKRNPRIRLIEGAYIHSDQGAHYTSPTYQKLVKKLNLGQSMSRRGNCWDNAPQESFFGHLKDEA
HIKPCASFNELKQEIKKYMTYYNHYRYQWNLKKMTPVGYRNHLLDVA
LERELTLEEKYARLEAERNLLKAENELLKKNQTYGREDEKEITLPSSQKFILIRSVIMKYNLRNMVSYLCKIAGVSRSGYYNYFSISSQEQRKQKSDRDE
ILKETILKALRFRNRKKGARQIKMTLAGQFQVVYNLKRIRRIMKKYEIVCPVRKANPYKRMLKATKEHRIVPNQLNREFKQNTPGKTLLTDITYLVYGKN
QRAYLSTILDGSTNEILAYHVSEQMTLELVTTTLHKLKRNPRIRLIEGAYIHSDQGAHYTSPTYQKLVKKLNLGQSMSRRGNCWDNAPQESFFGHLKDEA
HIKPCASFNELKQEIKKYMTYYNHYRYQWNLKKMTPVGYRNHLLDVA
Blast result :
Comments
ISBce14 is 78% (ORFA) and 78% (ORFB) aa similar to ISClte1. The third ORF is a putative ORFAB transposase reconstructed in silico by -1 frameshift.
References
1] Han,C.S., Xie,G., Challacombe,J.F., Altherr,M.R., Bhotika,S.S., Brown,N., Bruce,D., Campbell,C.S., Campbell,M.L., Chen,J., Chertkov,O., Cleland,C., Dimitrijevic,M., Doggett,N.A., Fawcett,J.J., Glavina,T., Goodwin,L.A., Green,L.D., Hill,K.K., Hitchcock,P., Jackson,P.J., Keim,P., Kewalramani,A.R., Longmire,J., Lucas,S., Malfatti,S., McMurry,K., Meincke,L.J., Misra,M., Moseman,B.L., Mundt,M., Munk,A.C., Okinaka,R.T., Parson-Quintana,B., Reilly,L.P., Richardson,P., Robinson,D.L., Rubin,E., Saunders,E., Tapia,R., Tesmer,J.G., Thayer,N., Thompson,L.S., Tice,H., Ticknor,L.O., Wills,P.L., Brettin,T.S. and Gilna,P. (2006) J. Bacteriol. 188 (9), 3382-3390.