ISFnu8
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AABF02000025 | ND | Fusobacterium nucleatum | Fusobacterium nucleatum subsp. vincentii ATCC 49256 |
DNA section
IS Length : 1446 bp
Ends
IR Length : 22/25
IRL : TGTACTGCACCCAAAATCTTGGACACAAGATTGAAGGTGCAGTTTTTTTG
IRR : TGTACTGACCCCAAAAAGTTGGACAAATTTAATTTAACTTACTAATAAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGGGGGCTGTTGC | TATCATTTTGAAGATTCTTG | 0 |
DNA sequence
TGTACTGCACCCAAAATCTTGGACACAAGATTGAAGGTGCAGTTTTTTTGTGAGTAAATTAACAAAAGAAGATAAAATTGAAATATATGAAAGAAGGTTA
AAAGGTGAAACCATTCCTGCATTGGCTAAAACTTTTAATATTACTGAATCTAAAATTAAATACTTTATTGCTTTAATTAAAAAACATGGATATACTATCT
TAAGAAATGGTAAAAATAGAATTTATTCCAAAGAATTTAAATTACAAGCAATTAATAGAATTCTAAGTAATTATGAGTCTGTTAATTCTGTTGCTATTGA
TATTGGGTTGCCTTCTGATACTATTTTACATAATTGGCTTTTAAAATTTAAAGAAAACGGGTATAATGTTGTAGAAAAGAAAAAAGGAAGGAAACCTAAA
GTTATGACTAAACCTAAGGAAAATAACAAAGAGTTATCTGAAAAAGAAAAAATTAAAAAATTAGAAGAAGAAAATTTGTATCTTAAAGCTGAGAATGAAT
ACTTAAAAAAATTGAGAGCTCTAGTTCAAGAGAGGGCGCTAAAAGAGAAGAAAAAGTAAAAGTAATAGCCGAATTTAGAGCTAAATACCCTTTCAAAATG
TTATTAAAAATTGCTGAGATAGCAAAATCTGTATATTATTATTACATCAATAAAAAAGACATTGATGAAAAAAATATAAATATTATTGAAAAAATCAAAG
AAATTTACTATGCGAATAGTGGAAGATATGGTTATCGCAGAGTAACATTAGAACTAAAAAATCAAGGTTTTAATATTAATCATAAAAAAGTACAAAGACT
TATGAAGAAATTTAATTTACAAAGTATTATTCGTAAAAAGAGAAAATATTCTTCATACAAAGGTCAAGTAGGTAAAATAGCTGATAATCATATCAAGAGA
GATTTTGAAGCCACAGCTCCAAATCAAAAATGGTTTACAGATGTAACAGAATTCAATTTAAGAGGAGAAAAGCTATATTTATCTCCAATATTAGATGCTT
ATGGAAGATACATAGTTTCATATGATATTTCGCGAAGTCCTAACTTAGAGCAGATAAATCATATGTTAAATTTAGCATTTAAAGAAAATGAGAATTATGA
GAATTTAATATTTCATAGTGATCAAGGTTGGCAATATCAGCATAATTCATATCAGAAAAGGTTAAAAGAAAAGAAGATAACTCAAAGTATGTCAAGAAAA
GGAAATAGTTTAGATAATGGATTAATGGAATGTTTCTTTGGATTATTAAAATCAGAAATGTTTTATGAACAAGAAGCAAAGTACAAAACACTGGAAGAAT
TAGAAAAAGCAATAGAAGATTATATATATTATTACAATAACAAAAGAATAAAGGAAAAACTAAAAGGATTAACTCCTGCTTCTTACAGAAGTCAATCCTT
ATTAGTAAGTTAAATTAAATTTGTCCAACTTTTTGGGGTCAGTACA
AAAGGTGAAACCATTCCTGCATTGGCTAAAACTTTTAATATTACTGAATCTAAAATTAAATACTTTATTGCTTTAATTAAAAAACATGGATATACTATCT
TAAGAAATGGTAAAAATAGAATTTATTCCAAAGAATTTAAATTACAAGCAATTAATAGAATTCTAAGTAATTATGAGTCTGTTAATTCTGTTGCTATTGA
TATTGGGTTGCCTTCTGATACTATTTTACATAATTGGCTTTTAAAATTTAAAGAAAACGGGTATAATGTTGTAGAAAAGAAAAAAGGAAGGAAACCTAAA
GTTATGACTAAACCTAAGGAAAATAACAAAGAGTTATCTGAAAAAGAAAAAATTAAAAAATTAGAAGAAGAAAATTTGTATCTTAAAGCTGAGAATGAAT
ACTTAAAAAAATTGAGAGCTCTAGTTCAAGAGAGGGCGCTAAAAGAGAAGAAAAAGTAAAAGTAATAGCCGAATTTAGAGCTAAATACCCTTTCAAAATG
TTATTAAAAATTGCTGAGATAGCAAAATCTGTATATTATTATTACATCAATAAAAAAGACATTGATGAAAAAAATATAAATATTATTGAAAAAATCAAAG
AAATTTACTATGCGAATAGTGGAAGATATGGTTATCGCAGAGTAACATTAGAACTAAAAAATCAAGGTTTTAATATTAATCATAAAAAAGTACAAAGACT
TATGAAGAAATTTAATTTACAAAGTATTATTCGTAAAAAGAGAAAATATTCTTCATACAAAGGTCAAGTAGGTAAAATAGCTGATAATCATATCAAGAGA
GATTTTGAAGCCACAGCTCCAAATCAAAAATGGTTTACAGATGTAACAGAATTCAATTTAAGAGGAGAAAAGCTATATTTATCTCCAATATTAGATGCTT
ATGGAAGATACATAGTTTCATATGATATTTCGCGAAGTCCTAACTTAGAGCAGATAAATCATATGTTAAATTTAGCATTTAAAGAAAATGAGAATTATGA
GAATTTAATATTTCATAGTGATCAAGGTTGGCAATATCAGCATAATTCATATCAGAAAAGGTTAAAAGAAAAGAAGATAACTCAAAGTATGTCAAGAAAA
GGAAATAGTTTAGATAATGGATTAATGGAATGTTTCTTTGGATTATTAAAATCAGAAATGTTTTATGAACAAGAAGCAAAGTACAAAACACTGGAAGAAT
TAGAAAAAGCAATAGAAGATTATATATATTATTACAATAACAAAAGAATAAAGGAAAAACTAAAAGGATTAACTCCTGCTTCTTACAGAAGTCAATCCTT
ATTAGTAAGTTAAATTAAATTTGTCCAACTTTTTGGGGTCAGTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 510 bp | 169 aa | 50 | 559 | + | No |
Description : First part of the transposase
ORF sequence :
VSKLTKEDKIEIYERRLKGETIPALAKTFNITESKIKYFIALIKKHGYTILRNGKNRIYSKEFKLQAINRILSNYESVNSVAIDIGLPSDTILHNWLLKF
KENGYNVVEKKKGRKPKVMTKPKENNKELSEKEKIKKLEEENLYLKAENEYLKKLRALVQERALKEKKK
KENGYNVVEKKKGRKPKVMTKPKENNKELSEKEKIKKLEEENLYLKAENEYLKKLRALVQERALKEKKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 915 bp | 304 aa | 499 | 1413 | + | No |
Description : Second part of the transposase
ORF sequence :
ILKKIESSSSREGAKREEKVKVIAEFRAKYPFKMLLKIAEIAKSVYYYYINKKDIDEKNINIIEKIKEIYYANSGRYGYRRVTLELKNQGFNINHKKVQR
LMKKFNLQSIIRKKRKYSSYKGQVGKIADNHIKRDFEATAPNQKWFTDVTEFNLRGEKLYLSPILDAYGRYIVSYDISRSPNLEQINHMLNLAFKENENY
ENLIFHSDQGWQYQHNSYQKRLKEKKITQSMSRKGNSLDNGLMECFFGLLKSEMFYEQEAKYKTLEELEKAIEDYIYYYNNKRIKEKLKGLTPASYRSQS
LLVS
LMKKFNLQSIIRKKRKYSSYKGQVGKIADNHIKRDFEATAPNQKWFTDVTEFNLRGEKLYLSPILDAYGRYIVSYDISRSPNLEQINHMLNLAFKENENY
ENLIFHSDQGWQYQHNSYQKRLKEKKITQSMSRKGNSLDNGLMECFFGLLKSEMFYEQEAKYKTLEELEKAIEDYIYYYNNKRIKEKLKGLTPASYRSQS
LLVS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1364 bp | 454 aa | 50 | 1413 | + | Yes |
Chemistry : DDE
ORF sequence :
VSKLTKEDKIEIYERRLKGETIPALAKTFNITESKIKYFIALIKKHGYTILRNGKNRIYSKEFKLQAINRILSNYESVNSVAIDIGLPSDTILHNWLLKF
KENGYNVVEKKKGRKPKVMTKPKENNKELSEKEKIKKLEEENLYLKAENEYLKKLRALVQERALKEKKKVKVIAEFRAKYPFKMLLKIAEIAKSVYYYYI
NKKDIDEKNINIIEKIKEIYYANSGRYGYRRVTLELKNQGFNINHKKVQRLMKKFNLQSIIRKKRKYSSYKGQVGKIADNHIKRDFEATAPNQKWFTDVT
EFNLRGEKLYLSPILDAYGRYIVSYDISRSPNLEQINHMLNLAFKENENYENLIFHSDQGWQYQHNSYQKRLKEKKITQSMSRKGNSLDNGLMECFFGLL
KSEMFYEQEAKYKTLEELEKAIEDYIYYYNNKRIKEKLKGLTPASYRSQSLLVS
KENGYNVVEKKKGRKPKVMTKPKENNKELSEKEKIKKLEEENLYLKAENEYLKKLRALVQERALKEKKKVKVIAEFRAKYPFKMLLKIAEIAKSVYYYYI
NKKDIDEKNINIIEKIKEIYYANSGRYGYRRVTLELKNQGFNINHKKVQRLMKKFNLQSIIRKKRKYSSYKGQVGKIADNHIKRDFEATAPNQKWFTDVT
EFNLRGEKLYLSPILDAYGRYIVSYDISRSPNLEQINHMLNLAFKENENYENLIFHSDQGWQYQHNSYQKRLKEKKITQSMSRKGNSLDNGLMECFFGLL
KSEMFYEQEAKYKTLEELEKAIEDYIYYYNNKRIKEKLKGLTPASYRSQSLLVS
Blast result :
Comments
Genome in progress.
ISFnu8 is 92% aa similar to ISFnu6.
The third ORF is putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISFnu8 is 92% aa similar to ISFnu6.
The third ORF is putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Kapatral,V., Ivanova,N., Anderson,I., Reznik,G., Bhattacharyya,A., Gardner,W.L., Mikhailova,N., Lapidus,A., Larsen,N., D'Souza,M., Walunas,T., Haselkorn,R., Overbeek,R. and Kyrpides,N. (2003) Genome Res. 13 (6), 1180-1189.