ISSth6
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008532 | ND | Streptococcus thermophilus | Streptococcus thermophilus LMD-9 |
DNA section
IS Length : 1427 bp
Ends
IR Length : 24/30
IRL : TGAACTGCACCCCAAAAGTTAGACAGAAAAAATCTAACTTTCGGGGTGTT
IRR : TGTACTGACCCCAAAAAGTTAGACAAATAATTTAAGTAAAGGATTTAGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACAATCATGCAAAAAGCAACGGTT | TTA | TGCCGTTGCTTTTTGGAGCT | 3 |
DNA sequence
TGAACTGCACCCCAAAAGTTAGACAGAAAAAATCTAACTTTCGGGGTGTTTTTATTATGAAATTGACTTATGAAGATAAGGTTCAAATCTATGAACTTAG
AAACCAAGGATATAGCTTAGATCAGCTTTCAAATAAATTTGGGATAAACATTTCTAATCTTAGCTACATGATTAAATTGATTGATCGATACGGAATAGAA
ATCGCTAAAAAAGGAAAGAATCGTTACTACTCTCCTGAATTAAAACAAGAAATGATTGATAAAGTCCTATATAAAGGCTATTCTCAAGATAGAGTTTCTC
TTGAATATGCTCTCCCAAATCGTGGGACGCTTCCTAACTGGCTCGCACACTATAAGAAAAACGGGTATACTATTGTTGAGAAAACAAGAGGAAGGTTATC
TAAAATGGGACGTAAGCCAAAGAAAAAACTGGAAGAAATGGAACGTCTTCAAGCAGAAAATGAGTACCTTAGAGCGGAGAATGCAGTTCTTAAAAAGTTG
AGAGAACTCCGATTGAGGGAAGAAAAAGAGCAAGAAGAAAAACGGAAATTGTCCGAGGATTGGTAACTGAGTTTTCTTTAGATATGCTTCTAAAGATTAT
TAAGCTTGCTCGTTCAACCTATTATTACCACTTAAAACAGCTGGATCAAAATGATAAGGATTATGACATTAAAGCTGAAATTCAAGCCATTTTTACTGAG
CATAAAGGAAATTACGGCTATCGTCGAATGACTCTTGAATTGAGAAATCGAGGAATTGTGATTAATCATAAGAGAGTTCAACGTCTTATGAAAGTACTTG
GTTTAACCGCTAGAATTCGCCGTAAACGTAAGTATTCTTCATATCAAGGAGAGGTTGGTATGAAAGCAGAGAATCTCATCCAACGCCAGTTCGAAGCATC
CAAACCATTGGAAAAATGCTACACAGATGTAACAGAATTTGCCATTCCAGCAAGCAGCCAAAAACTCTATTTGTCGCCAATTTTAGATGGCTTCAATAGT
GAAATTATCGCCTACAATCTTTCTAAGTCACCGAACTTGATACAAGTGAAAGCTATGTTAGAACAAGCCTTTACAGAGAAACATTACAAAAATACAATTC
TCCATAGTGACCAGGGATGGCAATATCAACATGATTTCTATCATCATTTTTTAGAGGAGAAAGGAATCCAACCATCCATGTCACGTAAAGGCAATAGTCC
AGACAACGGCATGATGGAATCCTTCTTTGGCATTCTTAAGTCTGAGATGTTTTATGGTTATGAGAAGACGTTTAAGTCAATTGAGCATTTGGAACAAGCA
ATTGCAGACTATATTAATTATTACAACAACAAACGCATTAAGGTAAAACTAAAAGGACTCAGTCCTGTACAATACAGAACTAAATCCTTTACTTAAATTA
TTTGTCTAACTTTTTGGGGTCAGTACA
AAACCAAGGATATAGCTTAGATCAGCTTTCAAATAAATTTGGGATAAACATTTCTAATCTTAGCTACATGATTAAATTGATTGATCGATACGGAATAGAA
ATCGCTAAAAAAGGAAAGAATCGTTACTACTCTCCTGAATTAAAACAAGAAATGATTGATAAAGTCCTATATAAAGGCTATTCTCAAGATAGAGTTTCTC
TTGAATATGCTCTCCCAAATCGTGGGACGCTTCCTAACTGGCTCGCACACTATAAGAAAAACGGGTATACTATTGTTGAGAAAACAAGAGGAAGGTTATC
TAAAATGGGACGTAAGCCAAAGAAAAAACTGGAAGAAATGGAACGTCTTCAAGCAGAAAATGAGTACCTTAGAGCGGAGAATGCAGTTCTTAAAAAGTTG
AGAGAACTCCGATTGAGGGAAGAAAAAGAGCAAGAAGAAAAACGGAAATTGTCCGAGGATTGGTAACTGAGTTTTCTTTAGATATGCTTCTAAAGATTAT
TAAGCTTGCTCGTTCAACCTATTATTACCACTTAAAACAGCTGGATCAAAATGATAAGGATTATGACATTAAAGCTGAAATTCAAGCCATTTTTACTGAG
CATAAAGGAAATTACGGCTATCGTCGAATGACTCTTGAATTGAGAAATCGAGGAATTGTGATTAATCATAAGAGAGTTCAACGTCTTATGAAAGTACTTG
GTTTAACCGCTAGAATTCGCCGTAAACGTAAGTATTCTTCATATCAAGGAGAGGTTGGTATGAAAGCAGAGAATCTCATCCAACGCCAGTTCGAAGCATC
CAAACCATTGGAAAAATGCTACACAGATGTAACAGAATTTGCCATTCCAGCAAGCAGCCAAAAACTCTATTTGTCGCCAATTTTAGATGGCTTCAATAGT
GAAATTATCGCCTACAATCTTTCTAAGTCACCGAACTTGATACAAGTGAAAGCTATGTTAGAACAAGCCTTTACAGAGAAACATTACAAAAATACAATTC
TCCATAGTGACCAGGGATGGCAATATCAACATGATTTCTATCATCATTTTTTAGAGGAGAAAGGAATCCAACCATCCATGTCACGTAAAGGCAATAGTCC
AGACAACGGCATGATGGAATCCTTCTTTGGCATTCTTAAGTCTGAGATGTTTTATGGTTATGAGAAGACGTTTAAGTCAATTGAGCATTTGGAACAAGCA
ATTGCAGACTATATTAATTATTACAACAACAAACGCATTAAGGTAAAACTAAAAGGACTCAGTCCTGTACAATACAGAACTAAATCCTTTACTTAAATTA
TTTGTCTAACTTTTTGGGGTCAGTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 510 bp | 169 aa | 57 | 566 | + | No |
Description : First part of the transposase
ORF sequence :
MKLTYEDKVQIYELRNQGYSLDQLSNKFGINISNLSYMIKLIDRYGIEIAKKGKNRYYSPELKQEMIDKVLYKGYSQDRVSLEYALPNRGTLPNWLAHYK
KNGYTIVEKTRGRLSKMGRKPKKKLEEMERLQAENEYLRAENAVLKKLRELRLREEKEQEEKRKLSEDW
KNGYTIVEKTRGRLSKMGRKPKKKLEEMERLQAENEYLRAENAVLKKLRELRLREEKEQEEKRKLSEDW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 904 bp | 300 aa | 493 | 1396 | + | No |
Description : Second part of the transposase
ORF sequence :
KVERTPIEGRKRARRKTEIVRGLVTEFSLDMLLKIIKLARSTYYYHLKQLDQNDKDYDIKAEIQAIFTEHKGNYGYRRMTLELRNRGIVINHKRVQRLMK
VLGLTARIRRKRKYSSYQGEVGMKAENLIQRQFEASKPLEKCYTDVTEFAIPASSQKLYLSPILDGFNSEIIAYNLSKSPNLIQVKAMLEQAFTEKHYKN
TILHSDQGWQYQHDFYHHFLEEKGIQPSMSRKGNSPDNGMMESFFGILKSEMFYGYEKTFKSIEHLEQAIADYINYYNNKRIKVKLKGLSPVQYRTKSFT
VLGLTARIRRKRKYSSYQGEVGMKAENLIQRQFEASKPLEKCYTDVTEFAIPASSQKLYLSPILDGFNSEIIAYNLSKSPNLIQVKAMLEQAFTEKHYKN
TILHSDQGWQYQHDFYHHFLEEKGIQPSMSRKGNSPDNGMMESFFGILKSEMFYGYEKTFKSIEHLEQAIADYINYYNNKRIKVKLKGLSPVQYRTKSFT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1340 bp | 446 aa | 57 | 1396 | + | Yes |
Chemistry : DDE
ORF sequence :
MKLTYEDKVQIYELRNQGYSLDQLSNKFGINISNLSYMIKLIDRYGIEIAKKGKNRYYSPELKQEMIDKVLYKGYSQDRVSLEYALPNRGTLPNWLAHYK
KNGYTIVEKTRGRLSKMGRKPKKKLEEMERLQAENEYLRAENAVLKKVERTPIEGRKRARRKTEIVRGLVTEFSLDMLLKIIKLARSTYYYHLKQLDQND
KDYDIKAEIQAIFTEHKGNYGYRRMTLELRNRGIVINHKRVQRLMKVLGLTARIRRKRKYSSYQGEVGMKAENLIQRQFEASKPLEKCYTDVTEFAIPAS
SQKLYLSPILDGFNSEIIAYNLSKSPNLIQVKAMLEQAFTEKHYKNTILHSDQGWQYQHDFYHHFLEEKGIQPSMSRKGNSPDNGMMESFFGILKSEMFY
GYEKTFKSIEHLEQAIADYINYYNNKRIKVKLKGLSPVQYRTKSFT
KNGYTIVEKTRGRLSKMGRKPKKKLEEMERLQAENEYLRAENAVLKKVERTPIEGRKRARRKTEIVRGLVTEFSLDMLLKIIKLARSTYYYHLKQLDQND
KDYDIKAEIQAIFTEHKGNYGYRRMTLELRNRGIVINHKRVQRLMKVLGLTARIRRKRKYSSYQGEVGMKAENLIQRQFEASKPLEKCYTDVTEFAIPAS
SQKLYLSPILDGFNSEIIAYNLSKSPNLIQVKAMLEQAFTEKHYKNTILHSDQGWQYQHDFYHHFLEEKGIQPSMSRKGNSPDNGMMESFFGILKSEMFY
GYEKTFKSIEHLEQAIADYINYYNNKRIKVKLKGLSPVQYRTKSFT
Blast result :
Comments
ISSth6 is 93% aa similar to ISSmu1.
References
1] Makarova,K., Slesarev,A., Wolf,Y., Sorokin,A., Mirkin,B., Koonin,E., Pavolv,A., Pavlova,N., Karamychev,V., Polouchine,N., Shakhova,V., Grigoriev,I., Lou,Y., Rohksar,D., Lucas,S., Huang,K., Goldstein,D.M., Hawkins,T., Plengvidhya,V., Welker,D., Hughes,J., Goh,Y., Benson,A., Baldwin,K., Lee,J.-H., Diaz-Muniz,I., Dosti,B., Smeianov,V., Wechter,W., Barabote,R., Lorca,G., Altermann,E., Barrangou,R., Ganesan,B., Xie,Y., Rawsthorne,H., Tamir,D., Parker,C., Breidt,F., Broadbent,J., Hutkins,R., O'Sulllivan,D., Steele,J., Unlu,G., Saier,M., Klaenhammer,T., Richardson,P., Kozyavkin,S., Weimer,B. and Mills,D. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (42), 15611-15616.