ISfinder Mirror Site

ISCro1

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Citrobacter rodentium	Citrobacter rodentium ICC168

DNA section

IS Length : 2699 bp

Ends

IR Length : 16/22

IRL : GTAAGCGTCTCATTTAAACCGTCTGGTCTGTTTCCTCCGGCTCCACAAAA
IRR : GTAAGCGGCTCGCCAGAACCGTATTGATATTTACTGAGAGCTCAGATCAA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTTTCGGATAAG	CCTCGTCC	TGTGGGCTAATACTCT	8

DNA sequence

GTAAGCGTCTCATTTAAACCGTCTGGTCTGTTTCCTCCGGCTCCACAAAAATAATGTCCATCATTTTTAGTGGACACTATCGTATGGAATACCGGACCTG
GATTACTGAAGCTTTACGCCTTCACTTCGAAGAACATTTACCTCGGGTTGTGGCCGGACGTCGCCTGGGTGTACCAAAATCAACAGTTTGTAGTATGTTC
GTGCGCTTTCGGAGAGCTGGCCTTTCGTGGCCTTTGCCCGCAGGCATGTCGGAGCAGGAACTTGATGCCTGCCTTTACGGACAATTTTCCACGGTACCAG
TCGTACGTCCTGAAAGCACCGTTATATCCGAAACCCCCGTGGTAAAAAAACGTCCCCGGCGGCCCAACTTCCCTTATGAGTTTAAAATCGCCTTAGTGGA
GCAGTCACTGCAGCCCGGAGCCTGTGTGGCGCAGATCGCCCGGGAAAACGGAATCAACGATAACCTGCTCTTCAACTGGCGCCATCAATACCGGAAAGGT
GGCCTGCTGCCTTCCGGAAAAAATATGCCGGCACTGCTTCCCGTGACGTTAACGCCGGAGCCGGATAATAAAATCCCGGCCCCCGCACAGGAACCAGAGC
AGATAAATACACCGTCCGACAGTCTGTGTTGTGAGCTGGTTCTGCCGGCCGGAACTCTCAGGCTTAAAGGTAAACTGACGCCGGCGTTATTACAGACACT
TATCCGCGAAATAAAAGGGAGCAGCCACTGATGATATCTCTCCCTGCAGGTTCGCGTATCTGGCTGGTTGCAGGTATCACCGATATGCGAAATGGCTTTA
ACGGCCTGGCATCAAAAGTTCAGAACGTCCTGAAGGATGACCCGTTCTCCGGACACCTGTTCATCTTCCGCGGACGCCGGGGTGACCAGATAAAAGTGTT
GTGGGCTGACAGTGACGGACTGTGCCTCTTCACCAAACGCCTGGAGCGGGGCCGCTTCATCTGGCCAGTCACCCGTGACGGCAAGGTGCACCTTACTCCG
GCTCAGTTATCCATGCTTCTTGAAGGTATCAACTGGAAGCACCCGAAACGAACGGAACGCGCTGGAATCCGCATATAACCCGTTGTAAAGTGAGGATATG
GACACCTCACTTGCTCATGAGAACGCCCGCCTGCGGGCACTGTTGCAGACGCAACAGGACACCATCCGCCAGATGGCCGAATACAACCGCCTGCTCTCAC
AGCGGGTGGCGGCTTATGCTTCCGAAATCAACCGGCTGAAGGCGCTGGTTGCGAAACTGCAACGTATGCAGTTCGGTAAAAGCTCAGAAAAACTTCGCGC
AAAAACCGAACGGCAGATACAGGATGCACAGGAGAGAATCAGCGCACTTCAGGAAGAAATGGCTGAAACGCTGGGTGAGCAATATGACCCGGCACTGCCA
TCCGCCCTGCGCCAGTCTTCAGCCCGTAAACCGTTACCGGCCTCACTTCCCCGTGAAACCCGGGTTATCCGGCCGGAAGAGGAATGCTGTCCTGCCTGTG
GTGGTGAACTCAGTTCTCTGGGATGTGATGTGTCAGAGCAACTGGAGCTTATCAGCAGCGCCTTTAAGGTTATCGAAACACAACGTCCGAAACTGGCCTG
TTGCCGGTGCGACCATATCGTGCAGGCACCAGTACCTTCAAAACCCATTGCACGCAGTTATGCCGGAGCGGGGCTTCTGGCCCATGTTGTCACCGGGAAA
TATGCAGACCATCTGCCGTTATACCGCCAGTCAGAAATATACCGTCGTCAGGGAGTGGAGCTGAGCCGTGCCACACTGGGGCGCTGGACAGGTGCTGTTG
CTGAACTGCTGGAGCCGCTGTATGACGTCCTGCGCCAGTATGTGCTGATGCCCGGTAAAGTCCATGCTGATGATATCCCCGTCCCGGTCCAGGAGCCGGG
CAGCGGTAAAACCCGGACAGCCCGGCTGTGGGTCTACGTCCGTGATGACCGTAACGCCGGTTCACAGATGCCCCCGGCGGTCTGGTTCGCGTACAGCCCG
GACCGGAAAGGCATACATCCACAGAATCACCTGTCCGGTTACAGCGGAGTGCTTCAGGCCGATGCTTACGGTGGCTACCGGGCGTTATACGAATCCGGCA
GAATAACGGAAGCCGCGTGTATGGCCCATGCCCGGAGAAAAATCCACGATGTGCATGCAAGAGCGCCAACCGATATCACCACGGAAGCCCTGCAGCGTAT
CGGTGAACTGTATGCCATCGAAGCAGAAGTCCGGGGATGTTCAGCAGAACAGCGTCTGGCGGCAAGAAAAGCCAGAGCTGCGTCACTGATGCAGTCACTG
TATGACTGGATACAGACTCAGATGAAAACACTGTCGCGTCACTCGGATACGGCAAAAGCGTTCGCATACCTGCTGAAACAGTGGGATAGCCTGAACGTGT
ACTGCAGTAATGGCTGGGTGGAAATCGACAACAACATCGCAGAGAACGCCTTAAGGGGAGTGGCCGTAGGCCGGAAAAACTGGCTGTTCGCGGGTTCTGA
CAGCGGTGGCGAACATGCGGCGGTGTTGTACTCGCTGATCGGCACATGCCGTCTGAACAATGTGGAGCCAGAAAAATGGCTGCGTTACGTCATTGAGCAT
ATCCAGGACTGGCCGGCAAATCGGGTACGCGATCTGTTGCCCTGGAAAGTTGATCTGAGCTCTCAGTAAATATCAATACGGTTCTGGCGAGCCGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
678 bp	225 aa	54	731	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSIIFSGHYRMEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISETPVVKKR
PRRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAG
TLRLKGKLTPALLQTLIREIKGSSH

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
348 bp	115 aa	731	1078	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLFIFRGRRGDQIKVLWADSDGLCLFTKRLERGRFIWPVTRDGKVHLTPAQLSMLLEGI
NWKHPKRTERAGIRI

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1571 bp	523 aa	1098	2668	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEINRLKALVAKLQRMQFGKSSEKLRAKTERQIQDAQERISALQEEMAETLGEQYDPAL
PSALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIETQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTG
KYADHLPLYRQSEIYRRQGVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYVRDDRNAGSQMPPAVWFAYS
PDRKGIHPQNHLSGYSGVLQADAYGGYRALYESGRITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKARAASLMQS
LYDWIQTQMKTLSRHSDTAKAFAYLLKQWDSLNVYCSNGWVEIDNNIAENALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIE
HIQDWPANRVRDLLPWKVDLSSQ

Blast result :

Comments

21 identical copies in C. rodentium chromosome, plus 3 remnants. An example of a complete ISCro1 element is found in the C. rodentium genome sequence at co-ordinates 2992292-2994990.
ISCro1 is 54% (ORFA) aa similar to ISSfl3, 72% (ORFB) to ISBcen14 and 72% (ORFC) to ISEc8.

References

1] Nicola Petty (2007) Direct submission.