ISPpu19
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AB238971 | ND | Pseudomonas putida | Pseudomonas putida MT53 plasmid pWW53 |
DNA section
IS Length : 2377 bp
Ends
IR Length : 23
IRL : GTATCCGTCCGGCCAAGTCATCTACCCGGCCCCGCAAAGCCAGGACATGG
IRR : GTATGCGTCCGGGCATCACGTCTTGCGTGATTAAACCGGCTGCCACTTAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TACCTGGTGC | GATGCTAC | CAGTGTGCTT | 8 |
DNA sequence
GTATCCGTCCGGCCAAGTCATCTACCCGGCCCCGCAAAGCCAGGACATGGTGTGCACTTAAATTTAAGTGGACACCAGTTCTAGCCTTTTAAGCGGGTAT
TTCATGCAGCCGACACGCCGTTCGTATTCCAAATCCTTCAAGGCCCGGGTCATTCAAGAGTGTGTCCAACCCGGCGCTTCGATTGCCAGCGTTGCCCTTA
GCCATAGCCTCAACGCGAACCTCGTCCACAAATGGATTCGGGTGCAAGCGCAGAAAAGCACGGCGCTCCAACCTGCATTCATCCCGTTGCCTATACACGA
AACAGCCCACGGATCCTCAGCGAATATCAGCATTGAGATTCTGCACCCGCGCGGCACCGTCAAAGTGAACTGGCCAACCGATAATGCTGCTGCCTGTGCC
GCATTCCTTCGAGACCTTTTGCGATGATCCGCATCGACGCCATCTGGCTTGCCACCGAGCCCATGGACATGCGCGCCGGAACTGAAACGGCGCTGGCCAG
AGTGATCGCGGTGTTCGGTGCGGCGAAGCCGCACTGCGCCTATCTGTTTACCAATCGCCGTGCGACGCGGGTCAAAGTACTGGTACACGATGGGATTGGC
GTCTGGCTGGCGGCACGTCGTTTGAACCAAGGTAAATTCCATTGGCCAGGTGTTCGGCAAGGCAGTGAATTGCAGTTGGATACAGAGCAACTTCAGGCCC
TGTTGCTTGGCCTGCCTTGGCAACGGGTTGGCGCTGGCGGTGCAATCACGGTGCTTTAGAAACTGTAATTAGCATCTTGGTTTGCTGTCGCAGACGGTCT
GCTTTGGTAAAATCCAAGGCATGACTTCCTCGCCTAATCTCGATCAAATGACTCCCGACCAACTGCGTGCATTCGCTGCGCAATTGCTGTCGCAAGTCGA
GACCATGGGCAAGAAGATCAACCGCGATCAAACGGTGATCGAGAAGCTGACCCACGAGATCGCACAGCTCAAGCGTTTGAAGTTTGCCAAGCGCAGCGAG
CAGATGAGTCCTGATCAGGCCAGCCTGCTTGATGACCTGATCGATACTGATATCACGGCGATTGAGGCCGAGCTTCAGGCGTTGCAAATAGCACCAGCAG
CGACCGAGGAAAAGCAAAAGCCCAAGCGCACGGCATTGCCGGCAGAGTTCCCACGCACGTTGATCCATCACGAGCCGGACAACACTCACTGCCCATGCGG
CTGCGCACTGAAGCGCATCGGTGAAGATGTCAGTGAAAAGCTGGACTACACGCCGGGTGTGTTTACCGTTGAGCGCCATGTTCGTGGCAAGTGGGTTTGC
GATGACTGCGAAACGCTGATCCAGGCGCCGGTGCCGGCGCAGGTCATCGACAAGGGCATCCCGACGGCCGGGTTACTTGCTCACGTCATGATTGCGAAGT
TCGCTGACCATCTTCCGCTTTACCGTCAGGAATCGATTTTTGGTCGTGCTGGCTTGGCGATTCCACGCTCCACGTTGGCTCAGTGGGTTGGCATGACAGG
CGTGCAGTTGCAGCCCCTGGTCGATGCCTTGCGGGGCGTAGTGATCGGGCAACAGGTCATGCACGCGGATGAAACACCCGTGCAAATGCTTGCTCCAGGC
TCGAAAAAAACTCACCGATCTTATGTTTGGGCATACGCCACCAGCCAGTTCTGCGAGACAGCCGCTGTCGTCTACGACTTCAGCCCCAGCCGCGCTGGCG
AGCATGCTCGTAACTTCCTGCAAGGCTGGAAAGGCAAGTTGGTCTGCGATGATTTTGGCGGTTACAAGGCCAGCTTCGAACTGGGCGTGACCGAGATCGG
CTGCATGGCCCATGCCCGGCGCAAGTTCTTCGAGTTGCACGCAACCAACAAGAGCCAGATCGCCGAGCAGGCCCTGCGCTACATCCAGCTATTGTACGAA
GTCGAGAGCGAAGTGCGCGACCTGGAGCCGAATTTACGGCGCCGAATACGGCAAGAAAAAGCCGTACCGGTGATGGATAGGTTGCATGCCTGGATGATCG
CGCAGCGTGATGTTGTGCCCGAAGGGTCGGCTATCAGCAGAGCACTCGATTACAGCCTCAAACGCTGGGCAGCGCTGTCGCGCTACCTCGATGACGGGGC
GGTTCCCATTGATAATAATTGGGCGGAGAACCAGATCCGGCCATGGGCTCTTGGACGCAAGAACTGGCTCTTCGCCGGGTCACTACGCAGCGGAAAACGG
GCTGCGGCGATCATGAGTTTGATCCAGTCAGCACGGCTGAATGGCCATGATCCGTACGCCTATCTAAAGGATGTTCTCACGCGCCTGCCGACGCAGCGGG
CGAGTGAAATTGAGCAGTTGCTTCCGCATAAGTGGCAGCCGGTTTAATCACGCAAGACGTGATGCCCGGACGCATAC
TTCATGCAGCCGACACGCCGTTCGTATTCCAAATCCTTCAAGGCCCGGGTCATTCAAGAGTGTGTCCAACCCGGCGCTTCGATTGCCAGCGTTGCCCTTA
GCCATAGCCTCAACGCGAACCTCGTCCACAAATGGATTCGGGTGCAAGCGCAGAAAAGCACGGCGCTCCAACCTGCATTCATCCCGTTGCCTATACACGA
AACAGCCCACGGATCCTCAGCGAATATCAGCATTGAGATTCTGCACCCGCGCGGCACCGTCAAAGTGAACTGGCCAACCGATAATGCTGCTGCCTGTGCC
GCATTCCTTCGAGACCTTTTGCGATGATCCGCATCGACGCCATCTGGCTTGCCACCGAGCCCATGGACATGCGCGCCGGAACTGAAACGGCGCTGGCCAG
AGTGATCGCGGTGTTCGGTGCGGCGAAGCCGCACTGCGCCTATCTGTTTACCAATCGCCGTGCGACGCGGGTCAAAGTACTGGTACACGATGGGATTGGC
GTCTGGCTGGCGGCACGTCGTTTGAACCAAGGTAAATTCCATTGGCCAGGTGTTCGGCAAGGCAGTGAATTGCAGTTGGATACAGAGCAACTTCAGGCCC
TGTTGCTTGGCCTGCCTTGGCAACGGGTTGGCGCTGGCGGTGCAATCACGGTGCTTTAGAAACTGTAATTAGCATCTTGGTTTGCTGTCGCAGACGGTCT
GCTTTGGTAAAATCCAAGGCATGACTTCCTCGCCTAATCTCGATCAAATGACTCCCGACCAACTGCGTGCATTCGCTGCGCAATTGCTGTCGCAAGTCGA
GACCATGGGCAAGAAGATCAACCGCGATCAAACGGTGATCGAGAAGCTGACCCACGAGATCGCACAGCTCAAGCGTTTGAAGTTTGCCAAGCGCAGCGAG
CAGATGAGTCCTGATCAGGCCAGCCTGCTTGATGACCTGATCGATACTGATATCACGGCGATTGAGGCCGAGCTTCAGGCGTTGCAAATAGCACCAGCAG
CGACCGAGGAAAAGCAAAAGCCCAAGCGCACGGCATTGCCGGCAGAGTTCCCACGCACGTTGATCCATCACGAGCCGGACAACACTCACTGCCCATGCGG
CTGCGCACTGAAGCGCATCGGTGAAGATGTCAGTGAAAAGCTGGACTACACGCCGGGTGTGTTTACCGTTGAGCGCCATGTTCGTGGCAAGTGGGTTTGC
GATGACTGCGAAACGCTGATCCAGGCGCCGGTGCCGGCGCAGGTCATCGACAAGGGCATCCCGACGGCCGGGTTACTTGCTCACGTCATGATTGCGAAGT
TCGCTGACCATCTTCCGCTTTACCGTCAGGAATCGATTTTTGGTCGTGCTGGCTTGGCGATTCCACGCTCCACGTTGGCTCAGTGGGTTGGCATGACAGG
CGTGCAGTTGCAGCCCCTGGTCGATGCCTTGCGGGGCGTAGTGATCGGGCAACAGGTCATGCACGCGGATGAAACACCCGTGCAAATGCTTGCTCCAGGC
TCGAAAAAAACTCACCGATCTTATGTTTGGGCATACGCCACCAGCCAGTTCTGCGAGACAGCCGCTGTCGTCTACGACTTCAGCCCCAGCCGCGCTGGCG
AGCATGCTCGTAACTTCCTGCAAGGCTGGAAAGGCAAGTTGGTCTGCGATGATTTTGGCGGTTACAAGGCCAGCTTCGAACTGGGCGTGACCGAGATCGG
CTGCATGGCCCATGCCCGGCGCAAGTTCTTCGAGTTGCACGCAACCAACAAGAGCCAGATCGCCGAGCAGGCCCTGCGCTACATCCAGCTATTGTACGAA
GTCGAGAGCGAAGTGCGCGACCTGGAGCCGAATTTACGGCGCCGAATACGGCAAGAAAAAGCCGTACCGGTGATGGATAGGTTGCATGCCTGGATGATCG
CGCAGCGTGATGTTGTGCCCGAAGGGTCGGCTATCAGCAGAGCACTCGATTACAGCCTCAAACGCTGGGCAGCGCTGTCGCGCTACCTCGATGACGGGGC
GGTTCCCATTGATAATAATTGGGCGGAGAACCAGATCCGGCCATGGGCTCTTGGACGCAAGAACTGGCTCTTCGCCGGGTCACTACGCAGCGGAAAACGG
GCTGCGGCGATCATGAGTTTGATCCAGTCAGCACGGCTGAATGGCCATGATCCGTACGCCTATCTAAAGGATGTTCTCACGCGCCTGCCGACGCAGCGGG
CGAGTGAAATTGAGCAGTTGCTTCCGCATAAGTGGCAGCCGGTTTAATCACGCAAGACGTGATGCCCGGACGCATAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 324 bp | 107 aa | 104 | 427 | + | No |
AG : IS66 TnpA
ORF sequence :
MQPTRRSYSKSFKARVIQECVQPGASIASVALSHSLNANLVHKWIRVQAQKSTALQPAFIPLPIHETAHGSSANISIEILHPRGTVKVNWPTDNAAACAA
FLRDLLR
FLRDLLR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 336 bp | 111 aa | 424 | 759 | + | No |
AG : IS66 TnpB
ORF sequence :
MIRIDAIWLATEPMDMRAGTETALARVIAVFGAAKPHCAYLFTNRRATRVKVLVHDGIGVWLAARRLNQGKFHWPGVRQGSELQLDTEQLQALLLGLPWQ
RVGAGGAITVL
RVGAGGAITVL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1527 bp | 508 aa | 821 | 2347 | + | No |
Chemistry : DDE
ORF sequence :
MTSSPNLDQMTPDQLRAFAAQLLSQVETMGKKINRDQTVIEKLTHEIAQLKRLKFAKRSEQMSPDQASLLDDLIDTDITAIEAELQALQIAPAATEEKQK
PKRTALPAEFPRTLIHHEPDNTHCPCGCALKRIGEDVSEKLDYTPGVFTVERHVRGKWVCDDCETLIQAPVPAQVIDKGIPTAGLLAHVMIAKFADHLPL
YRQESIFGRAGLAIPRSTLAQWVGMTGVQLQPLVDALRGVVIGQQVMHADETPVQMLAPGSKKTHRSYVWAYATSQFCETAAVVYDFSPSRAGEHARNFL
QGWKGKLVCDDFGGYKASFELGVTEIGCMAHARRKFFELHATNKSQIAEQALRYIQLLYEVESEVRDLEPNLRRRIRQEKAVPVMDRLHAWMIAQRDVVP
EGSAISRALDYSLKRWAALSRYLDDGAVPIDNNWAENQIRPWALGRKNWLFAGSLRSGKRAAAIMSLIQSARLNGHDPYAYLKDVLTRLPTQRASEIEQL
LPHKWQPV
PKRTALPAEFPRTLIHHEPDNTHCPCGCALKRIGEDVSEKLDYTPGVFTVERHVRGKWVCDDCETLIQAPVPAQVIDKGIPTAGLLAHVMIAKFADHLPL
YRQESIFGRAGLAIPRSTLAQWVGMTGVQLQPLVDALRGVVIGQQVMHADETPVQMLAPGSKKTHRSYVWAYATSQFCETAAVVYDFSPSRAGEHARNFL
QGWKGKLVCDDFGGYKASFELGVTEIGCMAHARRKFFELHATNKSQIAEQALRYIQLLYEVESEVRDLEPNLRRRIRQEKAVPVMDRLHAWMIAQRDVVP
EGSAISRALDYSLKRWAALSRYLDDGAVPIDNNWAENQIRPWALGRKNWLFAGSLRSGKRAAAIMSLIQSARLNGHDPYAYLKDVLTRLPTQRASEIEQL
LPHKWQPV
Blast result :
Comments
ISPpu19 localized on plasmid pWW53. ORF3 contains DDE motif and exhibits 76% amino acid identity with ISPpu14 ORF3
References
1] Yano, H., Tsuda, M. (2005) Direct Submission
2] Yano,H., Garruto,C.E., Sota,M., Ohtsubo,Y., Nagata,Y., Zylstra,G.J., Williams,P.A. and Tsuda,M.(2007) J. Mol. Biol. 369 (1), 11-26
2] Yano,H., Garruto,C.E., Sota,M., Ohtsubo,Y., Nagata,Y., Zylstra,G.J., Williams,P.A. and Tsuda,M.(2007) J. Mol. Biol. 369 (1), 11-26