ISApl2
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Actinobacillus pleuropneumoniae | Actinobacillus pleuropneumoniae |
DNA section
IS Length : 1418 bp
Ends
IR Length : 22/25
IRL : TGATCTGCCCCCCAAAAGTTGGACTAAACCTCCAACGGATTAAGGTGCAG
IRR : TGATCTGACCCCAAAAAGTTAGACTGTTTAATTTAAGGACTGAGCCCTGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GA | TAA | TC | 3 |
DNA sequence
TGATCTGCCCCCCAAAAGTTGGACTAAACCTCCAACGGATTAAGGTGCAGATTTTTTATGACTAAATATAACTCGCTATTCAAACAACAAGTCATCGAGT
TTTATCTTCAAAATGACAAAAACCGTTTATTCACTCAACGACATTTTCAATTATCCAAGAAAACCTTAACTCGATGGATTGCTCAATTTAATCATAACGG
AATCAATGGGCTAGCGGTGATAGGTAAGAAGCAAAAATATTCCCCCGAATTCAAATTAACCGTCATACAAGCGGTCAAAAAGGGGCAATTTTCTGCGGAA
AGCGCCTCCCTTCATTTTGGCATTGCCAATTCAGGTTCGATTAGCCAATGGTTGCACATCGTTGAAGAACAAAGTATAAACGGCTTATTACCCAAGCCCA
AAGGTCGTCCGACAATGAAACCGAAATACCCCAAAATGCCTCCTCCACCTAAAACTGAAGAAGAACGCTTACGTTATCGTATTTTAGAGCTGGAAGCGGA
GAATGCTTACCTAAAAAAGTTGCGGAAGTTCAACCAACAAAAAATGCGGCAAAAGCAGCCATCGTAAAAACGTTGCGAGTGGATTTTCCCTTAGAAATCC
TCCTCCCTTTGACTGGTTTAAAACGTAGTACGTTCTTTTATCACTTACCGCCTAAAACCGATAAAAATGTAATGATTAGGCAGAAAGTGATTGAGATTTA
TCAAGATAATGATGGCAATTACGGCTATCGTCGTATTACGTTAAAGTTACGTGATTTTTTGGGTGCGATTAACCGTAAGCGTATTCAAAGGATAATGCAA
CAGCTTGATTTAAAAGGGAAATATAAGCAGAGGAAATATCGTTCTTATCAAGGTGAAGTGGGTAAAATTGCCGAGAATGTATTGCAACGGGATTTTCACG
CAACGGCGCCAAATGAAAAGCTCGTCACGGATATCACCGAGTTTAAATGTGCGGAAGGTAAGCTCTATTTATCGCCTATTAAGGACTTGTTTAATAGTGA
AATTCTTGCTTATGATTTAGCAAGAAGCCCTAACTTTGAGCAAGTGATGCGGATGCTAAAGCAAGCGGTCGCAAAACTGCCTAAGGACGCTAAACCGATT
TTACATTCCGACCAAGGCTGGCAATACCGGATGGCAGACTATCAAGACATGCTGAGAAAACATCATATTAAACAAAGTATGTCGAGAAAAGCGAATTGCT
TGGATAATGGGGCAATGGAAAGTTTTTTTGGGCGATTGAAAACGGAATGTTACTTTGGTAAGCGATTTGAAACTTTCGAACAACTTGAGAAAGTGATTCA
CGAGTACATTCATTATTACAACAATGAGCGTATTCAAGTGAAATTAAAAGGACTGAGCCCTGTACAATACAGGGCTCAGTCCTTAAATTAAACAGTCTAA
CTTTTTGGGGTCAGATCA
TTTATCTTCAAAATGACAAAAACCGTTTATTCACTCAACGACATTTTCAATTATCCAAGAAAACCTTAACTCGATGGATTGCTCAATTTAATCATAACGG
AATCAATGGGCTAGCGGTGATAGGTAAGAAGCAAAAATATTCCCCCGAATTCAAATTAACCGTCATACAAGCGGTCAAAAAGGGGCAATTTTCTGCGGAA
AGCGCCTCCCTTCATTTTGGCATTGCCAATTCAGGTTCGATTAGCCAATGGTTGCACATCGTTGAAGAACAAAGTATAAACGGCTTATTACCCAAGCCCA
AAGGTCGTCCGACAATGAAACCGAAATACCCCAAAATGCCTCCTCCACCTAAAACTGAAGAAGAACGCTTACGTTATCGTATTTTAGAGCTGGAAGCGGA
GAATGCTTACCTAAAAAAGTTGCGGAAGTTCAACCAACAAAAAATGCGGCAAAAGCAGCCATCGTAAAAACGTTGCGAGTGGATTTTCCCTTAGAAATCC
TCCTCCCTTTGACTGGTTTAAAACGTAGTACGTTCTTTTATCACTTACCGCCTAAAACCGATAAAAATGTAATGATTAGGCAGAAAGTGATTGAGATTTA
TCAAGATAATGATGGCAATTACGGCTATCGTCGTATTACGTTAAAGTTACGTGATTTTTTGGGTGCGATTAACCGTAAGCGTATTCAAAGGATAATGCAA
CAGCTTGATTTAAAAGGGAAATATAAGCAGAGGAAATATCGTTCTTATCAAGGTGAAGTGGGTAAAATTGCCGAGAATGTATTGCAACGGGATTTTCACG
CAACGGCGCCAAATGAAAAGCTCGTCACGGATATCACCGAGTTTAAATGTGCGGAAGGTAAGCTCTATTTATCGCCTATTAAGGACTTGTTTAATAGTGA
AATTCTTGCTTATGATTTAGCAAGAAGCCCTAACTTTGAGCAAGTGATGCGGATGCTAAAGCAAGCGGTCGCAAAACTGCCTAAGGACGCTAAACCGATT
TTACATTCCGACCAAGGCTGGCAATACCGGATGGCAGACTATCAAGACATGCTGAGAAAACATCATATTAAACAAAGTATGTCGAGAAAAGCGAATTGCT
TGGATAATGGGGCAATGGAAAGTTTTTTTGGGCGATTGAAAACGGAATGTTACTTTGGTAAGCGATTTGAAACTTTCGAACAACTTGAGAAAGTGATTCA
CGAGTACATTCATTATTACAACAATGAGCGTATTCAAGTGAAATTAAAAGGACTGAGCCCTGTACAATACAGGGCTCAGTCCTTAAATTAAACAGTCTAA
CTTTTTGGGGTCAGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 537 bp | 169 aa | 58 | 594 | + | No |
Description : First part of the transposase
ORF sequence :
MTKYNSLFKQQVIEFYLQNDKNRLFTQRHFQLSKKTLTRWIAQFNHNGINGLAVIGKKQKYSPEFKLTVIQAVKKGQFSAESASLHFGIANSGSISQWLH
IVEEQSINGLLPKPKGRPTMKPKYPKMPPPPKTEEERLRYRILELEAENAYLKKLRKFNQQKMRQKQPS
IVEEQSINGLLPKPKGRPTMKPKYPKMPPPPKTEEERLRYRILELEAENAYLKKLRKFNQQKMRQKQPS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 933 bp | 310 aa | 459 | 1391 | + | No |
Description : Second part of the transposase
ORF sequence :
RRTLTLSYFRAGSGECLPKKVAEVQPTKNAAKAAIVKTLRVDFPLEILLPLTGLKRSTFFYHLPPKTDKNVMIRQKVIEIYQDNDGNYGYRRITLKLRDF
LGAINRKRIQRIMQQLDLKGKYKQRKYRSYQGEVGKIAENVLQRDFHATAPNEKLVTDITEFKCAEGKLYLSPIKDLFNSEILAYDLARSPNFEQVMRML
KQAVAKLPKDAKPILHSDQGWQYRMADYQDMLRKHHIKQSMSRKANCLDNGAMESFFGRLKTECYFGKRFETFEQLEKVIHEYIHYYNNERIQVKLKGLS
PVQYRAQSLN
LGAINRKRIQRIMQQLDLKGKYKQRKYRSYQGEVGKIAENVLQRDFHATAPNEKLVTDITEFKCAEGKLYLSPIKDLFNSEILAYDLARSPNFEQVMRML
KQAVAKLPKDAKPILHSDQGWQYRMADYQDMLRKHHIKQSMSRKANCLDNGAMESFFGRLKTECYFGKRFETFEQLEKVIHEYIHYYNNERIQVKLKGLS
PVQYRAQSLN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1334 bp | 444 aa | 58 | 1391 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKYNSLFKQQVIEFYLQNDKNRLFTQRHFQLSKKTLTRWIAQFNHNGINGLAVIGKKQKYSPEFKLTVIQAVKKGQFSAESASLHFGIANSGSISQWLH
IVEEQSINGLLPKPKGRPTMKPKYPKMPPPPKTEEERLRYRILELEAENAYLKKVAEVQPTKNAAKAAIVKTLRVDFPLEILLPLTGLKRSTFFYHLPPK
TDKNVMIRQKVIEIYQDNDGNYGYRRITLKLRDFLGAINRKRIQRIMQQLDLKGKYKQRKYRSYQGEVGKIAENVLQRDFHATAPNEKLVTDITEFKCAE
GKLYLSPIKDLFNSEILAYDLARSPNFEQVMRMLKQAVAKLPKDAKPILHSDQGWQYRMADYQDMLRKHHIKQSMSRKANCLDNGAMESFFGRLKTECYF
GKRFETFEQLEKVIHEYIHYYNNERIQVKLKGLSPVQYRAQSLN
IVEEQSINGLLPKPKGRPTMKPKYPKMPPPPKTEEERLRYRILELEAENAYLKKVAEVQPTKNAAKAAIVKTLRVDFPLEILLPLTGLKRSTFFYHLPPK
TDKNVMIRQKVIEIYQDNDGNYGYRRITLKLRDFLGAINRKRIQRIMQQLDLKGKYKQRKYRSYQGEVGKIAENVLQRDFHATAPNEKLVTDITEFKCAE
GKLYLSPIKDLFNSEILAYDLARSPNFEQVMRMLKQAVAKLPKDAKPILHSDQGWQYRMADYQDMLRKHHIKQSMSRKANCLDNGAMESFFGRLKTECYF
GKRFETFEQLEKVIHEYIHYYNNERIQVKLKGLSPVQYRAQSLN
Blast result :
Comments
ISApl2 is 61% aa similar to ISBcen10. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Zhuofei Xu (2007) Direct submission.