ISSru2
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007677 | ND | Salinibacter ruber | Salinibacter ruber DSM 13855 |
DNA section
IS Length : 808 bp
Ends
IR Length : 14/22
IRL : GGTAGCGGCTCAACACGTAGTGGAGCTCTCTGTTTTAATGATGTCGCTCT
IRR : GGTAGCGGGTCGATGTTAAGTGATGGCTTCGTTGTACCACTCCACGAACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACGCGCATGTTGTCCTCGAGAA | GAATTTCC | TCATGCCACCGGTAGCTGGCGT | 8 |
DNA sequence
GGTAGCGGCTCAACACGTAGTGGAGCTCTCTGTTTTAATGATGTCGCTCTTGGCTCACCAGTCCATCCAGTCCGATGATCAAAGAGACCCATGAGTGTAG
GGAGTGCGGCTCCTCGAACATTATCAAAAACGGCCACAGCGCGAGCGGCTCTCAGCAATACCACTGCAAGGATTGTGGGGCCCATAAGGTGCTCGATCCG
GAGCCGCGGGGCTATTCCGAGGAGGAGAAAGAGAAGATTCTCCGGGCTTACCGTGAACGCGGGTCAAAACGAGCAATCAGTCGCATTTTTGGGATCAGTC
GCAATACTCTGAACCGGTGGCTTGAAAAAAGGGACACGAAGCCGACTCAGTAGCTGAGGGCCTCCGGCCAGCCAGGGACAACGACACCCTTGAACTCGAC
GAATGCTGGACATACGTCCGAAAACGGTCGAACAAACGGTGGCTCTGGGTCGCTCTATGCCGTCGAACTCGACAGGTCGTAGCATTTGTGATCGGAGACC
GCTCGGCAAAAACCTGCGCACGGCTCTGGAGCCGGATTCCGGAGGGCTACCGGAAAGGCCGAAGTTTCAGCGACTTCTGGAAGTCCTATCGCCCAGTTTT
TGAAGACGATTCCAGCCATCGGCAGGTCGGAAAGTCCAGTGGCGAGTTGGCACATGTGGAACGGTTTTTTGGGCGACTGCGGCAAAAGCTGGCACGGTAC
GTCCGTCGGACGCGAGCGGCCTCCCAGTCAGAACGAATGCTCCATCTGACGACAAAACTGTTCGTGGAGTGGTACAACGAAGCCATCACTTAACATCGAC
CCGCTACC
GGAGTGCGGCTCCTCGAACATTATCAAAAACGGCCACAGCGCGAGCGGCTCTCAGCAATACCACTGCAAGGATTGTGGGGCCCATAAGGTGCTCGATCCG
GAGCCGCGGGGCTATTCCGAGGAGGAGAAAGAGAAGATTCTCCGGGCTTACCGTGAACGCGGGTCAAAACGAGCAATCAGTCGCATTTTTGGGATCAGTC
GCAATACTCTGAACCGGTGGCTTGAAAAAAGGGACACGAAGCCGACTCAGTAGCTGAGGGCCTCCGGCCAGCCAGGGACAACGACACCCTTGAACTCGAC
GAATGCTGGACATACGTCCGAAAACGGTCGAACAAACGGTGGCTCTGGGTCGCTCTATGCCGTCGAACTCGACAGGTCGTAGCATTTGTGATCGGAGACC
GCTCGGCAAAAACCTGCGCACGGCTCTGGAGCCGGATTCCGGAGGGCTACCGGAAAGGCCGAAGTTTCAGCGACTTCTGGAAGTCCTATCGCCCAGTTTT
TGAAGACGATTCCAGCCATCGGCAGGTCGGAAAGTCCAGTGGCGAGTTGGCACATGTGGAACGGTTTTTTGGGCGACTGCGGCAAAAGCTGGCACGGTAC
GTCCGTCGGACGCGAGCGGCCTCCCAGTCAGAACGAATGCTCCATCTGACGACAAAACTGTTCGTGGAGTGGTACAACGAAGCCATCACTTAACATCGAC
CCGCTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 75 | 353 | + | No |
Description : First part of the transposase
ORF sequence :
MIKETHECRECGSSNIIKNGHSASGSQQYHCKDCGAHKVLDPEPRGYSEEEKEKILRAYRERGSKRAISRIFGISRNTLNRWLEKRDTKPTQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 468 bp | 155 aa | 326 | 793 | + | No |
Description : Second part of the transposase
ORF sequence :
KKGHEADSVAEGLRPARDNDTLELDECWTYVRKRSNKRWLWVALCRRTRQVVAFVIGDRSAKTCARLWSRIPEGYRKGRSFSDFWKSYRPVFEDDSSHRQ
VGKSSGELAHVERFFGRLRQKLARYVRRTRAASQSERMLHLTTKLFVEWYNEAIT
VGKSSGELAHVERFFGRLRQKLARYVRRTRAASQSERMLHLTTKLFVEWYNEAIT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 719 bp | 239 aa | 75 | 793 | + | Yes |
Chemistry : DDE
ORF sequence :
MIKETHECRECGSSNIIKNGHSASGSQQYHCKDCGAHKVLDPEPRGYSEEEKEKILRAYRERGSKRAISRIFGISRNTLNRWLEKKGHEADSVAEGLRPA
RDNDTLELDECWTYVRKRSNKRWLWVALCRRTRQVVAFVIGDRSAKTCARLWSRIPEGYRKGRSFSDFWKSYRPVFEDDSSHRQVGKSSGELAHVERFFG
RLRQKLARYVRRTRAASQSERMLHLTTKLFVEWYNEAIT
RDNDTLELDECWTYVRKRSNKRWLWVALCRRTRQVVAFVIGDRSAKTCARLWSRIPEGYRKGRSFSDFWKSYRPVFEDDSSHRQVGKSSGELAHVERFFG
RLRQKLARYVRRTRAASQSERMLHLTTKLFVEWYNEAIT
Blast result :
Comments
ISSru2 is 95% aa similar to ISSru3.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Mongodin,E.F., Nelson,K.E., Daugherty,S., Deboy,R.T., Wister,J., Khouri,H., Weidman,J., Walsh,D.A., Papke,R.T., Sanchez Perez,G., Sharma,A.K., Nesbo,C.L., Macleod,D., Bapteste,E., Doolittle,W.F.,(2005) Proc. Natl. Acad. Sci. U.S.A. 102 (50), 18147-18152