ISAav5

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008752 ND Acidovorax avenae
Acidovorax avenae subsp. citrulli AAC00-1
DNA section
IS Length : 1028 bp

Ends


IR Length : 19/24

IRL : TGTCTCAATAGGTTGTTCATCCAGGCCCGACCTGGGAAGCTGTGGTTACC
IRR : TGTTGAGTCTCACGAGGTTGTTCACACCCGGAATGCGTGTGATGGGAGGC

Insertion site


Left flankDirect repeatRight flankDR Length
CCTGGGCCGCGCTAGTGCAAAAGCCG6

DNA sequence

TGTCTCAATAGGTTGTTCATCCAGGCCCGACCTGGGAAGCTGTGGTTACCAGGCCCCAGCAACTCAGGACAGGACACCGGATGAACAGTCATAAGCATGC
GCGATTGACCCGCGCAGGTCGAGCCCTTCTGGTCGACAGAGTGCTCCAGCAGAACTGGACGATGCGCCAAGCCAGCGAAGCCGCAGGCGTGAGCCTGCGC
ACCGGCTACAAGTGGATGGCCCGCTTTCGCAACGAAGGCCCCGATGGCTTGCTCGACCGCAGCTCCCGCCCGCACCGCAGCCCCCAGGCCTGCCCACCCG
AGCAGGTCCAGCTGTTCGAACAGCAGCGCCGCCAGCGCTTGCCCCTTTGGCGCATCGCCCGTGAGACCGGCCGCAGTCTGGCGACCGTAGCGCGCCACAT
GGACCGCATCGGCCTGAGCCGGCTCGCATCGCTGGAGCCACCGCAGCCCGTGCGTCGCTACGAGCGCGCCAGCCCGGGCGAACTGCTGCACATCGACACC
AAGCGGCTCGGCCGCATCCACGGCGTGGGCCACCGCATCACCGGCGACCGAACCCAGAACCGCAACCGTGGCATCGGTTGGGATGCGGTGCATCTGGCCA
TCGACGACCACTCCCGCGTGTCCTTCGCCCAGGTCCTGGCCGACGAGACAGCGCTGAGTTGCGTGCAGTTCCTGCGAGACGCGGTGGCCTACTACGCCAA
CATCGGCGTACGCATCGAGCGCGTCATGACCGACAACGGCTCTGGCTACAAAAACGCCTTCCGCGCGGCCTGCGAGGAATTGGGCATCCGCCACATCCGC
ACGCGGCCCTATACGCCCAAGACCAACGGCAAGGCCGAGCGATTCGTACAGACCAGCCTGCGGGAGTGGGCCTATGCCAGACCCTACGAAAGCTCAGCCC
AACGCACGGCTGCCCTGCAGCCCTTCATTGACGGCTACAACTGGTGCCGGCCACACTCCGCGCTCGGCCATCAGCCTCCCATCACACGCATTCCGGGTGT
GAACAACCTCGTGAGACTCAACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
943 bp315 aa811023+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNSHKHARLTRAGRALLVDRVLQQNWTMRQASEAAGVSLRTGYKWMARFRNEGPDGLLDRSSRPHRSPQACPPEQVQLFEQQRRQRLPLWRIARETGRSL
ATVARHMDRIGLSRLASLEPPQPVRRYERASPGELLHIDTKRLGRIHGVGHRITGDRTQNRNRGIGWDAVHLAIDDHSRVSFAQVLADETALSCVQFLRD
AVAYYANIGVRIERVMTDNGSGYKNAFRAACEELGIRHIRTRPYTPKTNGKAERFVQTSLREWAYARPYESSAQRTAALQPFIDGYNWCRPHSALGHQPP
ITRIPGVNNLVRLNS

 

Blast result :
Comments
ISAav5 is 88% aa similar to IS1002. The stop codon is in the DR.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Stahl,D. and Richardson,P.(2007) Direct Submission GenBank.