ISfinder Mirror Site

ISMysp4

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008146	ND	Mycobacterium sp.	Mycobacterium sp. MCS

DNA section

IS Length : 1095 bp

Ends

IR Length : 30/47

IRL : TGTACTGACCACGGACGTTGGTGACGGCGTGGCTTGGTGACATGACGAAG
IRR : TGTACTGACCTGACAGGTTAGGTACGCGGCTGGCGGGTGGTTGACCGCCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCCCGCGTC	GCTAGC	CGCTCGTCGC	6

DNA sequence

TGTACTGACCACGGACGTTGGTGACGGCGTGGCTTGGTGACATGACGAAGACCTCCGAGTGAAGTGCGAGCTGTCTAGGAACGCACTCATTCACCGGAGG
TCTTCGTGTCCCACCGTAATGCCCCGTTGTCCGAAACCGGTCGTCTGAGGCTGGCTCGCTGCGTCGTTGAGGAGGGCTGGCCGCTGCGCCGGGCTGCTGA
GCGGTACCAGGTCGCTGTGACCACTGCGGCCCGGTGGGCCAGCCGATACCGGGAGCTGGGTCCAGCCGGCATGGTCGATCGCAGCTCCCGGCCGCACCGC
AGTCCCAATCAGACGCCGACTCGCACCGAACGGCGGATCATCAAAGTCCGGGTACTGCGCCGGTGGGGACCTGCTCGGATCGGCTATCTGTTGGGCATTC
ATCCCTCCACTGTCCATCGGGTCCTGACCCGCTACGGGCTGGCCAAACTGTGCTGGTTAGACCGACCCACTGGCCGGGTTGTCCGACGCATCGAAACCTC
CCGGTGCGGGGAGCTGGTGCATGTCGATGTCAAGAAACTCGGCAAGATCCCCGCCGGTGGCGGCTGGCGAATGTTGGGCCGCACGATCGGCAACCGCAAC
TCCAAGGCCGATACGAGCTCGGGGCGTCGCAACAAGTACCGGACCCCGCTGCGCGGTCACCATTTTCTGCACACCGCGATCGACGCCCACTCGCGGTTGG
CCTACTCCGAACTCCTGGCCGATGAACGCAAAGAGACCTCGGCGGGGTTCTGGTTACGGGCCAACGCCTGGTTCGCTGAGTGTGGCATCACTGTGCGGAA
AGTGTTGACCGACAATGGCTCCTGCTACCGATCCCACGCCTTCCGCGACGCACTTGGCGGCATCGAGCACCGGCGCACCCGCCCGTACCGGCCGCAGACC
AACGGCAAGGTGGAGAGATTTCACCGCACCCTGGCCGATGAATGGGCCTACGTCCGGCTCTACACCAGCGACTCCGAACGCTGCGAGCAGTTCCCGATCT
GGCTGCACACCTACAATCACCACCGCGGCCACACCGCACTCGGCGGTCAACCACCCGCCAGCCGCGTACCTAACCTGTCAGGTCAGTACA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
990 bp	329 aa	106	1095	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSHRNAPLSETGRLRLARCVVEEGWPLRRAAERYQVAVTTAARWASRYRELGPAGMVDRSSRPHRSPNQTPTRTERRIIKVRVLRRWGPARIGYLLGIHP
STVHRVLTRYGLAKLCWLDRPTGRVVRRIETSRCGELVHVDVKKLGKIPAGGGWRMLGRTIGNRNSKADTSSGRRNKYRTPLRGHHFLHTAIDAHSRLAY
SELLADERKETSAGFWLRANAWFAECGITVRKVLTDNGSCYRSHAFRDALGGIEHRRTRPYRPQTNGKVERFHRTLADEWAYVRLYTSDSERCEQFPIWL
HTYNHHRGHTALGGQPPASRVPNLSGQYS

Blast result :

Comments

ISMysp4 is 76 % aa similar to ISAni1 . The stop codon is included in DR .

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Martinez,M., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Miller,C.D., Hughes,J.E., Anderson,A.J., Sims,R.C. and Richardson,P.(2006) Direct Submission GenBank.