ISfinder Mirror Site

ISRpa3

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_005296	ND	Rhodopseudomonas palustris	Rhodopseudomonas palustris CGA009

DNA section

IS Length : 1261 bp

Ends

IR Length : 39/48

IRL : TGTTCAGCCCGGAGACATGACCTACGGGTGTTCGGAGACATAGTCGACGC
IRR : TGTTCAGCCCGGAGACATAGTCGACACGTGTTCGGAGACATGACCGACAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATTGCCGGGTCGAG		CTTGACCCGGCAAT	0

DNA sequence

TGTTCAGCCCGGAGACATGACCTACGGGTGTTCGGAGACATAGTCGACGCATCATAGTGGCTGGATTTGGCGGTTGGAGGCCAAGTCCATGCCCTGGCGT
GAGGTGTCGGCAGTGGATCAGAGACGAGAGTTCGTCCGGCTTGCGATGCAGGAGGGAGCGAACCGGCGGGAGTTGTGCCGGCGGTTCGGCATTCATTGGA
CGACCGGCTACAAATGGTTGGAGCGATGCGCGGCCGGAGGTGATGTCGTCGACCTATCGCGGCGGCCGCACGAAAGCCCGCGGCAAACCTCGGCGGCGTG
CGAGGCACAGGTGCTGGCGGTTCGCGATGCGCATCCGGCGTGGGGGGCGCGCAAGATCGCTAGCTCGATGAAGCGGTCCGGACACAGCGCTCCGGCGGTG
TCGACGATCCACGAGATCCTGCGCCGGCACGGCCGGATCAAGTCTGCCGGCGGCGGTCCACCGGCGACGCTGCGGTTCGAGATGCCGGCGCCGAACATGC
TTTGGCAGATGGACTTCAAAGGCTGGGTTCGGCTCGGCAACGACGTCCGCTGCCATCCGCTGACCGTGGTGGACGATCACTCACGCTACGATCTGTGCCT
CCAGGCTTGCGCCGATCAGCGCGGCGAAACCGTGCAGGACAGGCTGCAAACGACGTTCCGGCACTACGGCTTGCCCGACACGATCTTCGTCGACAACGGC
TCGCCGTGGTCGGATAGTTCCGGCGAGCGCTGGACGTGGTTTTCGGTGTGGCTGCTCAAGCTCGGCATCAGCGTGATCCGCAGCCGTCCCTATCACCCGC
AGAGCCGCGGCAAGAACGAGCGCTTCCATCGCACGCTGGACGACGAGGTGTTTGCGCTGCGGCCCTTGCGCGACCTCGCCGAGGCGCAACGTGCCTTCGA
CTCCTGGCGCGAGGTGTACAACTTCGAGCGCCCTCACGAGTCGCTAGGCCAGCTGGTGCCTGCTGATCGCTACCAACCGAGCCGGCGTTCTCTGCCGGAC
CGCCTGCCGGCTCCAGAATACGATGAGCGCGACATCGTCCGCTCCGTCCCGAAAACCAAGGCCTATGTCAGCTTCAAAGGTCGTTTGTGGAAGGTGCCGC
AAGCCTTCGCCGGAGAGCGCTTGGCCATCAGGCCGCTCTCCACCGACGGCAAATACGGCGTGTTCTTCGCCGCCCACCAAGTCGCCACCATCGACTTGAC
CGGCGGGGAAAGTGTCGGTCATGTCTCCGAACACGTGTCGACTATGTCTCCGGGCTGAACA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1170 bp	389 aa	89	1258	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPWREVSAVDQRREFVRLAMQEGANRRELCRRFGIHWTTGYKWLERCAAGGDVVDLSRRPHESPRQTSAACEAQVLAVRDAHPAWGARKIASSMKRSGHS
APAVSTIHEILRRHGRIKSAGGGPPATLRFEMPAPNMLWQMDFKGWVRLGNDVRCHPLTVVDDHSRYDLCLQACADQRGETVQDRLQTTFRHYGLPDTIF
VDNGSPWSDSSGERWTWFSVWLLKLGISVIRSRPYHPQSRGKNERFHRTLDDEVFALRPLRDLAEAQRAFDSWREVYNFERPHESLGQLVPADRYQPSRR
SLPDRLPAPEYDERDIVRSVPKTKAYVSFKGRLWKVPQAFAGERLAIRPLSTDGKYGVFFAAHQVATIDLTGGESVGHVSEHVSTMSPG

Blast result :

Comments

ISRpa3 is 49% aa similar to ISMav2.

References

1] Larimer,F.W., Chain,P., Hauser,L., Lamerdin,J., Malfatti,S., Do,L., Land,M.L., Pelletier,D.A., Beatty,J.T., Lang,A.S., Tabita,F.R., Gibson,J.L., Hanson,T.E., Bobst,C., Torres,J.L., Peres,C., Harrison,F.H., Gibson,J. and Harwood,C.S.(2004) Nat. Biotechnol. 22 (1), 55-61.