ISCpr2
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005861 | ND | Candidatus Protochlamydia | Candidatus Protochlamydia amoebophila UWE25 |
DNA section
IS Length : 1299 bp
Ends
IR Length : 26/29
IRL : TGTAGTGCTCCCCAAATAAACCGGACCATCTGGCTTGCTTCTAAGATATA
IRR : TGGAGTTGCCCCTATAAAACCGGACCGCTAGCCTGCCATTTTTAATTTCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GATTGCTAAA | CCTATTTATCA | 0 |
DNA sequence
TGTAGTGCTCCCCAAATAAACCGGACCATCTGGCTTGCTTCTAAGATATAGGAGTAAGACTAGATTTATGACTAATTGTAAATTAATTACATCAGTAGAA
AGAAGAAGACGATGGTCCTTAGAAGAAAAAAAACAGATTATTGAAAAAACATATCAAGAAGGTCAATCTGTTTCCCAAATAGCAAGAAGATATGATATAA
CACCAAGTCAACTTTTTGCATGGAGAAGGCAAATGGAACAAGGAGCACTACAAGGAATTAGCAGCCGAGAAGAACTAGTTCCCAAAAGCCAAGTGAAAGA
AATGGAAAAGCGAATAAGAGAACTAGAGCGGATGCTTGGGAAAAAGACACTTGAAAATGAAATTTTAAAAGAGGCTGTAAGGCTTGGGCAGGAAAAAAAA
CTCATCTCGCGGCAGCCATTGCCAAGCTTAAGCGATTTAGCCTAAGGAAAATAGCAGAGGTGATGGAAGTATCAAGATCCAATCTTATAGAGCAGCTTAA
AAAGCATTTACCTCATCAGCCAATCTTTTATTCTAAAGCGGAAAATGAACAGGTTTTAGCTTTAATCAAAGAGGTGGTTAAAAACAGGCCGACTTACGGT
TATCGTAGGGTCCATGCGATTGTCAATAGCCTTCTCAAAGAGAAAAAACTTAAAGTCATTAATCATAAGCGGGTTTTTAGACTGATGAGGCAGCATCATC
TTTTGCTACAAAGGCCAAACAGGCGTCCTAAACGCTTACATACAGGCAAAGTAGAGACTCTCTACAGCAATACACGATGGTGCTCAGACAGCTTCTCTAT
TCAATGCTTAAATGGTGATCGTGTTCATGTCGCCTTCTCGTTGGATACATGTGACAGAGAAGTCATGCGTTATATTGCCTCTACAATAGGTATTGATGGG
CAGATGATTCGAGATTTGATGCTTGAGACTATTGAATATAGATTTGAACAGCCAAAAGCTAGAGTACCTCTTGAATGGCTCTCTGATAATGGCAGCTGTT
ACACGGCTAAAGAAACTGTCAATTTTGGTAGAATGCTTGGTTTAACCATTAGAACCACGCCACCTTATAGTCCAGAAAGTAATGGCATGGCAGAAGCTTT
TGTCAAAACTTTTAAAAGAGATTATGTTTATTTCGGCAATCTGGCAAGTGCTGAAGCTGTTTTGCAGCAATTGCCTATTTGGATCGAAGACTATAACGTT
AAAGCCCCTCACAAGGCTTTAAATATGCTCTCTCCAAGAGAGTATTTAAGGAAATTAAAAATGGCAGGCTAGCGGTCCGGTTTTATAGGGGCAACTCCA
AGAAGAAGACGATGGTCCTTAGAAGAAAAAAAACAGATTATTGAAAAAACATATCAAGAAGGTCAATCTGTTTCCCAAATAGCAAGAAGATATGATATAA
CACCAAGTCAACTTTTTGCATGGAGAAGGCAAATGGAACAAGGAGCACTACAAGGAATTAGCAGCCGAGAAGAACTAGTTCCCAAAAGCCAAGTGAAAGA
AATGGAAAAGCGAATAAGAGAACTAGAGCGGATGCTTGGGAAAAAGACACTTGAAAATGAAATTTTAAAAGAGGCTGTAAGGCTTGGGCAGGAAAAAAAA
CTCATCTCGCGGCAGCCATTGCCAAGCTTAAGCGATTTAGCCTAAGGAAAATAGCAGAGGTGATGGAAGTATCAAGATCCAATCTTATAGAGCAGCTTAA
AAAGCATTTACCTCATCAGCCAATCTTTTATTCTAAAGCGGAAAATGAACAGGTTTTAGCTTTAATCAAAGAGGTGGTTAAAAACAGGCCGACTTACGGT
TATCGTAGGGTCCATGCGATTGTCAATAGCCTTCTCAAAGAGAAAAAACTTAAAGTCATTAATCATAAGCGGGTTTTTAGACTGATGAGGCAGCATCATC
TTTTGCTACAAAGGCCAAACAGGCGTCCTAAACGCTTACATACAGGCAAAGTAGAGACTCTCTACAGCAATACACGATGGTGCTCAGACAGCTTCTCTAT
TCAATGCTTAAATGGTGATCGTGTTCATGTCGCCTTCTCGTTGGATACATGTGACAGAGAAGTCATGCGTTATATTGCCTCTACAATAGGTATTGATGGG
CAGATGATTCGAGATTTGATGCTTGAGACTATTGAATATAGATTTGAACAGCCAAAAGCTAGAGTACCTCTTGAATGGCTCTCTGATAATGGCAGCTGTT
ACACGGCTAAAGAAACTGTCAATTTTGGTAGAATGCTTGGTTTAACCATTAGAACCACGCCACCTTATAGTCCAGAAAGTAATGGCATGGCAGAAGCTTT
TGTCAAAACTTTTAAAAGAGATTATGTTTATTTCGGCAATCTGGCAAGTGCTGAAGCTGTTTTGCAGCAATTGCCTATTTGGATCGAAGACTATAACGTT
AAAGCCCCTCACAAGGCTTTAAATATGCTCTCTCCAAGAGAGTATTTAAGGAAATTAAAAATGGCAGGCTAGCGGTCCGGTTTTATAGGGGCAACTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 378 bp | 125 aa | 68 | 445 | + | No |
Description : First part of the transposase
ORF sequence :
MTNCKLITSVERRRRWSLEEKKQIIEKTYQEGQSVSQIARRYDITPSQLFAWRRQMEQGALQGISSREELVPKSQVKEMEKRIRELERMLGKKTLENEIL
KEAVRLGQEKKLISRQPLPSLSDLA
KEAVRLGQEKKLISRQPLPSLSDLA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 912 bp | 303 aa | 361 | 1272 | + | No |
Description : Second part of the transposase
ORF sequence :
NFKRGCKAWAGKKTHLAAAIAKLKRFSLRKIAEVMEVSRSNLIEQLKKHLPHQPIFYSKAENEQVLALIKEVVKNRPTYGYRRVHAIVNSLLKEKKLKVI
NHKRVFRLMRQHHLLLQRPNRRPKRLHTGKVETLYSNTRWCSDSFSIQCLNGDRVHVAFSLDTCDREVMRYIASTIGIDGQMIRDLMLETIEYRFEQPKA
RVPLEWLSDNGSCYTAKETVNFGRMLGLTIRTTPPYSPESNGMAEAFVKTFKRDYVYFGNLASAEAVLQQLPIWIEDYNVKAPHKALNMLSPREYLRKLK
MAG
NHKRVFRLMRQHHLLLQRPNRRPKRLHTGKVETLYSNTRWCSDSFSIQCLNGDRVHVAFSLDTCDREVMRYIASTIGIDGQMIRDLMLETIEYRFEQPKA
RVPLEWLSDNGSCYTAKETVNFGRMLGLTIRTTPPYSPESNGMAEAFVKTFKRDYVYFGNLASAEAVLQQLPIWIEDYNVKAPHKALNMLSPREYLRKLK
MAG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1205 bp | 401 aa | 68 | 1272 | + | Yes |
Chemistry : DDE
ORF sequence :
MTNCKLITSVERRRRWSLEEKKQIIEKTYQEGQSVSQIARRYDITPSQLFAWRRQMEQGALQGISSREELVPKSQVKEMEKRIRELERMLGKKTLENEIL
KEAVRLGQEKKTHLAAAIAKLKRFSLRKIAEVMEVSRSNLIEQLKKHLPHQPIFYSKAENEQVLALIKEVVKNRPTYGYRRVHAIVNSLLKEKKLKVINH
KRVFRLMRQHHLLLQRPNRRPKRLHTGKVETLYSNTRWCSDSFSIQCLNGDRVHVAFSLDTCDREVMRYIASTIGIDGQMIRDLMLETIEYRFEQPKARV
PLEWLSDNGSCYTAKETVNFGRMLGLTIRTTPPYSPESNGMAEAFVKTFKRDYVYFGNLASAEAVLQQLPIWIEDYNVKAPHKALNMLSPREYLRKLKMA
G
KEAVRLGQEKKTHLAAAIAKLKRFSLRKIAEVMEVSRSNLIEQLKKHLPHQPIFYSKAENEQVLALIKEVVKNRPTYGYRRVHAIVNSLLKEKKLKVINH
KRVFRLMRQHHLLLQRPNRRPKRLHTGKVETLYSNTRWCSDSFSIQCLNGDRVHVAFSLDTCDREVMRYIASTIGIDGQMIRDLMLETIEYRFEQPKARV
PLEWLSDNGSCYTAKETVNFGRMLGLTIRTTPPYSPESNGMAEAFVKTFKRDYVYFGNLASAEAVLQQLPIWIEDYNVKAPHKALNMLSPREYLRKLKMA
G
Blast result :
Comments
ISCpr2 is 77% (ORFA) aa similar to ISRso10 and 64% (ORFB) aa similar to IS426. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Horn,M., Collingro,A., Schmitz-Esser,S., Beier,C.L., Purkhold,U., Fartmann,B., Brandt,P., Nyakatura,G.J., Droege,M., Frishman,D., Rattei,T., Mewes,H.W. and Wagner,M. (2004) Science 304 (5671), 728-730.