ISRhsp2
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAME01000001 | ND | Rhodobacter sphaeroides | Rhodobacter sphaeroides ATCC 17025 |
DNA section
IS Length : 1295 bp
Ends
IR Length : 29/43
IRL : TGGCCCCCGTTTCGACCGGACACTTGGGCATAACCATGGAGGCTCAGGCA
IRR : TTGCCCCCGTTTCACCGGACACTCAGGCGGTTGCGGTTTGGGCTGCGATG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCTGAACTG | AAAACCAGGC | 0 |
DNA sequence
TGGCCCCCGTTTCGACCGGACACTTGGGCATAACCATGGAGGCTCAGGCATATGCCTGAGCACGTGCTTATGTCTGAGATAGAGATCATCACCGATGGCG
GCCGTCGGCGCCGCTGGACTGCCGCCGAGAAGGTGCGGATCGTCGAGGAGACGCTGGACGACCGGGCCAGCATCTCGGTCGTGGCGCGCCGCAATGGTGT
GGCGCCAAACCTGTTGTATCGTTGGCGGCGGCTCATGCTCGAAGGGGGGAGTGTCGCCGTGGCGGAAGACGATGACGTCACCAGCAACAGGGTCGTCCGC
CAGATGGAGGACCGCATCCGCGAACTTGAACGTCAGCTCGGCCGCAAGACGCTGGAGGTCGAGATCCTGCGCGAGGCCCTGGACAAGTCACGCTCAAAAA
AACCGACCTTGCTCGCGCGGTCGCCGCTGAGGGACGATTTCCAGTGAAGGCCGTGGCGGAAACCCTGGGCGTGGCCCGGTCCAACCTGATCGAGCGGCTG
CAAGGCAAGACAAAGCCGCGTCGGCGCTATCAGAAGGTGCAAGACGCGGCGGTGGTGCCAAGGATCACGGCGCTCGTGTCGGCGCGCCCGACCTATGGCT
ACCGGCGGATCACCGCGATCCTGAACCGCCAGTTGCGCTCGGAAGGCTTGGCACCGGTCAATCACAAGCGGGTCTACCGGATCATGCAGGCCCATAACCT
GCTGCTGGCCCGGAAATATTCCGAACGCCCCGAGCATGTTCACGACGGCAAGGTGATCGTCATGCGCTCCAACCTGCGCTGGTGCTCGGACGGGTTCGAG
TTCACCTGCTGGAACGGCGACATCGTCCGCGGCGCTTTCATCATCGATGCCCATGATCGGGAAATCATCGCATGGCGCGCCGTGGTGAACGCCGGGATCA
GCGGCTCCGACATCCGAGACATCATGCTCGAAGCCGTCGAACGCCGCTTCGGCGCATACCGCGCCCCATCGGTTATCGAGATGCTCTCCGACAACGGGCC
GCCCTACATCGCGAAGGACACCCAGATCTTCGCCCGCCAACTCGGTCTGAAGCCCTGCTTCACCCCGGTCCAGAGCCCGCAGAGCAACGGCATCTCGGAG
GCTTTCGTGAAGACGCTGAAGCGCGACTACGTCCAGGTGACGCCATTGCCCCACGCACAGACCGCTCTCGGATTGATCGGAGCCTGGATCGAGGATTACA
ACGAAAACCACCCGCACTCAGGGCTGAAAATGCGCTCGCCCCGCGAGTTCATCGCAGCCCAAACCGCAACCGCCTGAGTGTCCGGTGAAACGGGGGCAA
GCCGTCGGCGCCGCTGGACTGCCGCCGAGAAGGTGCGGATCGTCGAGGAGACGCTGGACGACCGGGCCAGCATCTCGGTCGTGGCGCGCCGCAATGGTGT
GGCGCCAAACCTGTTGTATCGTTGGCGGCGGCTCATGCTCGAAGGGGGGAGTGTCGCCGTGGCGGAAGACGATGACGTCACCAGCAACAGGGTCGTCCGC
CAGATGGAGGACCGCATCCGCGAACTTGAACGTCAGCTCGGCCGCAAGACGCTGGAGGTCGAGATCCTGCGCGAGGCCCTGGACAAGTCACGCTCAAAAA
AACCGACCTTGCTCGCGCGGTCGCCGCTGAGGGACGATTTCCAGTGAAGGCCGTGGCGGAAACCCTGGGCGTGGCCCGGTCCAACCTGATCGAGCGGCTG
CAAGGCAAGACAAAGCCGCGTCGGCGCTATCAGAAGGTGCAAGACGCGGCGGTGGTGCCAAGGATCACGGCGCTCGTGTCGGCGCGCCCGACCTATGGCT
ACCGGCGGATCACCGCGATCCTGAACCGCCAGTTGCGCTCGGAAGGCTTGGCACCGGTCAATCACAAGCGGGTCTACCGGATCATGCAGGCCCATAACCT
GCTGCTGGCCCGGAAATATTCCGAACGCCCCGAGCATGTTCACGACGGCAAGGTGATCGTCATGCGCTCCAACCTGCGCTGGTGCTCGGACGGGTTCGAG
TTCACCTGCTGGAACGGCGACATCGTCCGCGGCGCTTTCATCATCGATGCCCATGATCGGGAAATCATCGCATGGCGCGCCGTGGTGAACGCCGGGATCA
GCGGCTCCGACATCCGAGACATCATGCTCGAAGCCGTCGAACGCCGCTTCGGCGCATACCGCGCCCCATCGGTTATCGAGATGCTCTCCGACAACGGGCC
GCCCTACATCGCGAAGGACACCCAGATCTTCGCCCGCCAACTCGGTCTGAAGCCCTGCTTCACCCCGGTCCAGAGCCCGCAGAGCAACGGCATCTCGGAG
GCTTTCGTGAAGACGCTGAAGCGCGACTACGTCCAGGTGACGCCATTGCCCCACGCACAGACCGCTCTCGGATTGATCGGAGCCTGGATCGAGGATTACA
ACGAAAACCACCCGCACTCAGGGCTGAAAATGCGCTCGCCCCGCGAGTTCATCGCAGCCCAAACCGCAACCGCCTGAGTGTCCGGTGAAACGGGGGCAA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 396 bp | 131 aa | 52 | 447 | + | No |
Description : First part of the transposase
ORF sequence :
MPEHVLMSEIEIITDGGRRRRWTAAEKVRIVEETLDDRASISVVARRNGVAPNLLYRWRRLMLEGGSVAVAEDDDVTSNRVVRQMEDRIRELERQLGRKT
LEVEILREALDKSRSKKPTLLARSPLRDDFQ
LEVEILREALDKSRSKKPTLLARSPLRDDFQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 948 bp | 315 aa | 330 | 1277 | + | No |
Description : Second part of the transposase
ORF sequence :
TSARPQDAGGRDPARGPGQVTLKKTDLARAVAAEGRFPVKAVAETLGVARSNLIERLQGKTKPRRRYQKVQDAAVVPRITALVSARPTYGYRRITAILNR
QLRSEGLAPVNHKRVYRIMQAHNLLLARKYSERPEHVHDGKVIVMRSNLRWCSDGFEFTCWNGDIVRGAFIIDAHDREIIAWRAVVNAGISGSDIRDIML
EAVERRFGAYRAPSVIEMLSDNGPPYIAKDTQIFARQLGLKPCFTPVQSPQSNGISEAFVKTLKRDYVQVTPLPHAQTALGLIGAWIEDYNENHPHSGLK
MRSPREFIAAQTATA
QLRSEGLAPVNHKRVYRIMQAHNLLLARKYSERPEHVHDGKVIVMRSNLRWCSDGFEFTCWNGDIVRGAFIIDAHDREIIAWRAVVNAGISGSDIRDIML
EAVERRFGAYRAPSVIEMLSDNGPPYIAKDTQIFARQLGLKPCFTPVQSPQSNGISEAFVKTLKRDYVQVTPLPHAQTALGLIGAWIEDYNENHPHSGLK
MRSPREFIAAQTATA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1226 bp | 408 aa | 52 | 1277 | + | Yes |
Chemistry : DDE
ORF sequence :
MPEHVLMSEIEIITDGGRRRRWTAAEKVRIVEETLDDRASISVVARRNGVAPNLLYRWRRLMLEGGSVAVAEDDDVTSNRVVRQMEDRIRELERQLGRKT
LEVEILREALDKSRSKKTDLARAVAAEGRFPVKAVAETLGVARSNLIERLQGKTKPRRRYQKVQDAAVVPRITALVSARPTYGYRRITAILNRQLRSEGL
APVNHKRVYRIMQAHNLLLARKYSERPEHVHDGKVIVMRSNLRWCSDGFEFTCWNGDIVRGAFIIDAHDREIIAWRAVVNAGISGSDIRDIMLEAVERRF
GAYRAPSVIEMLSDNGPPYIAKDTQIFARQLGLKPCFTPVQSPQSNGISEAFVKTLKRDYVQVTPLPHAQTALGLIGAWIEDYNENHPHSGLKMRSPREF
IAAQTATA
LEVEILREALDKSRSKKTDLARAVAAEGRFPVKAVAETLGVARSNLIERLQGKTKPRRRYQKVQDAAVVPRITALVSARPTYGYRRITAILNRQLRSEGL
APVNHKRVYRIMQAHNLLLARKYSERPEHVHDGKVIVMRSNLRWCSDGFEFTCWNGDIVRGAFIIDAHDREIIAWRAVVNAGISGSDIRDIMLEAVERRF
GAYRAPSVIEMLSDNGPPYIAKDTQIFARQLGLKPCFTPVQSPQSNGISEAFVKTLKRDYVQVTPLPHAQTALGLIGAWIEDYNENHPHSGLKMRSPREF
IAAQTATA
Blast result :
Comments
Genome in progress.
ISRhsp2 is 72 % (ORFA) and 78% (ORFB) aa similar to IS426. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISRhsp2 is 72 % (ORFA) and 78% (ORFB) aa similar to IS426. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Richardson,P., Mackenzie,C., Choudhary,M., Donohue,T.J. and Kaplan,S. (2005) Direct submission GenBank.