ISSpwi1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAVK01000009 | ND | Sphingomonas wittichii | Sphingomonas wittichii RW1 |
DNA section
IS Length : 1269 bp
Ends
IR Length : 19/25
IRL : TGATCTGCCCCCTTCTGAGTGGTCCAAAATTGATGATATTTTGGATGACG
IRR : TGACCGGCCCCCACCGAGTGGTCCGAGCGGATTGTTAGTGCATTGACGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCAGGCTATT | TGAC | CTTCCACGTG | 4 |
DNA sequence
TGATCTGCCCCCTTCTGAGTGGTCCAAAATTGATGATATTTTGGATGACGAAGGAGACAAGGAATGCCGAGCAAGAAGCATCGCCCGGAAGAGATCATTG
GCAAGCTGCGTGAAGCTGAGGTTGTGCTGGCGCAGGGCGCCACGACTGCGGAGGCGTGTCGGCGGATCGCGGTCAGCGAGCAGACCTATTACCGGTGGCG
TAAGGAATATGGTGGCCTGAAGACCGATCAGGCGCGTCGGATGAAGGACCTGGAGAAAGAGAATGTGCGGCTTCGCCGCGCGATCTCGGATCTGACGCTC
GACAAGCTAATCCTGCAGGAGGCCGCCCGGGGAAACTTCTGAGCCCCGCGCGTCGCAGGCGCTGTATCGACCACATCAGAACGATGATGCCGGTGTCCGA
GCGGCGGCTGTGCCGTGTGCTCGGGCAATACCGATCGACACAGCGCAAAGCGCCTCGCGGGGCAGATGACGAAGCGGCTCTCACCGAGGATATCATTGCG
CTGGCGCGGCAATATGGTCGTTATGGCTATCGCCGGGTGACGGCGTTGCTGCGCGATGCGGGGTGGCATGTGAACCGCAAGCGGGTCGAGCGCATCTGGC
GACGCGAGGGGCTCAAGGTGCCGCAAAGGCAGCCGAAGCGCGGGCGGCTGTGGCTGAACGACGGATCGTGCATCCGGCTTCGGCCCGAGTATCCTGGCCA
TGTCTGGTCCTACGACTTTGTTGAGGGGCGGACGCACGATGGGCGTAAATACCGTATCCTGTCGATCATCGACGAGGCGAGCCGGGAGTGCATGGCGTTG
CCCGTGGCGCGCAAGCTTAAGAGCGACGATGTGCTGGCGGCGCTGGCCGAACTGTTCGTCACGCGCGGGCCACCAGCGCATATCAGATCCGACAATGGCC
CGGAGTTTATCGCTACGGCGGTACAAGAATGGCTGGCCAAGGTCGGCGTGAAGACGCTCTGCATTGCGCCCGGATCGCCATGGGAAAATGGCTATTGTGA
ATCGTTCAACGGGTCGCTGCGCGATGAACTGCTCAACGGCGAGATCTTCTACTCGCTAGCCGAGGCCCGGATCCTGATCGAAGCGTGGCGGCGACATTAC
AACACCGTCCGGCCGCATAGCTCGCTCGGGTATCGACCGCCCGCGCCGGAGGCCGTTCCATCTCCAGTGTCGCCCTCCGGTTCCGCTTCGCTCCACCTAC
GGCCAACACTGGAGATGGAGGCGTCAATGCACTAACAATCCGCTCGGACCACTCGGTGGGGGCCGGTCA
GCAAGCTGCGTGAAGCTGAGGTTGTGCTGGCGCAGGGCGCCACGACTGCGGAGGCGTGTCGGCGGATCGCGGTCAGCGAGCAGACCTATTACCGGTGGCG
TAAGGAATATGGTGGCCTGAAGACCGATCAGGCGCGTCGGATGAAGGACCTGGAGAAAGAGAATGTGCGGCTTCGCCGCGCGATCTCGGATCTGACGCTC
GACAAGCTAATCCTGCAGGAGGCCGCCCGGGGAAACTTCTGAGCCCCGCGCGTCGCAGGCGCTGTATCGACCACATCAGAACGATGATGCCGGTGTCCGA
GCGGCGGCTGTGCCGTGTGCTCGGGCAATACCGATCGACACAGCGCAAAGCGCCTCGCGGGGCAGATGACGAAGCGGCTCTCACCGAGGATATCATTGCG
CTGGCGCGGCAATATGGTCGTTATGGCTATCGCCGGGTGACGGCGTTGCTGCGCGATGCGGGGTGGCATGTGAACCGCAAGCGGGTCGAGCGCATCTGGC
GACGCGAGGGGCTCAAGGTGCCGCAAAGGCAGCCGAAGCGCGGGCGGCTGTGGCTGAACGACGGATCGTGCATCCGGCTTCGGCCCGAGTATCCTGGCCA
TGTCTGGTCCTACGACTTTGTTGAGGGGCGGACGCACGATGGGCGTAAATACCGTATCCTGTCGATCATCGACGAGGCGAGCCGGGAGTGCATGGCGTTG
CCCGTGGCGCGCAAGCTTAAGAGCGACGATGTGCTGGCGGCGCTGGCCGAACTGTTCGTCACGCGCGGGCCACCAGCGCATATCAGATCCGACAATGGCC
CGGAGTTTATCGCTACGGCGGTACAAGAATGGCTGGCCAAGGTCGGCGTGAAGACGCTCTGCATTGCGCCCGGATCGCCATGGGAAAATGGCTATTGTGA
ATCGTTCAACGGGTCGCTGCGCGATGAACTGCTCAACGGCGAGATCTTCTACTCGCTAGCCGAGGCCCGGATCCTGATCGAAGCGTGGCGGCGACATTAC
AACACCGTCCGGCCGCATAGCTCGCTCGGGTATCGACCGCCCGCGCCGGAGGCCGTTCCATCTCCAGTGTCGCCCTCCGGTTCCGCTTCGCTCCACCTAC
GGCCAACACTGGAGATGGAGGCGTCAATGCACTAACAATCCGCTCGGACCACTCGGTGGGGGCCGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 64 | 342 | + | No |
Description : First part of the transposase
ORF sequence :
MPSKKHRPEEIIGKLREAEVVLAQGATTAEACRRIAVSEQTYYRWRKEYGGLKTDQARRMKDLEKENVRLRRAISDLTLDKLILQEAARGNF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1032 bp | 343 aa | 204 | 1235 | + | No |
Description : Second part of the transposase
ORF sequence :
GIWWPEDRSGASDEGPGERECAASPRDLGSDARQANPAGGRPGKLLSPARRRRCIDHIRTMMPVSERRLCRVLGQYRSTQRKAPRGADDEAALTEDIIAL
ARQYGRYGYRRVTALLRDAGWHVNRKRVERIWRREGLKVPQRQPKRGRLWLNDGSCIRLRPEYPGHVWSYDFVEGRTHDGRKYRILSIIDEASRECMALP
VARKLKSDDVLAALAELFVTRGPPAHIRSDNGPEFIATAVQEWLAKVGVKTLCIAPGSPWENGYCESFNGSLRDELLNGEIFYSLAEARILIEAWRRHYN
TVRPHSSLGYRPPAPEAVPSPVSPSGSASLHLRPTLEMEASMH
ARQYGRYGYRRVTALLRDAGWHVNRKRVERIWRREGLKVPQRQPKRGRLWLNDGSCIRLRPEYPGHVWSYDFVEGRTHDGRKYRILSIIDEASRECMALP
VARKLKSDDVLAALAELFVTRGPPAHIRSDNGPEFIATAVQEWLAKVGVKTLCIAPGSPWENGYCESFNGSLRDELLNGEIFYSLAEARILIEAWRRHYN
TVRPHSSLGYRPPAPEAVPSPVSPSGSASLHLRPTLEMEASMH
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1172 bp | 390 aa | 64 | 1235 | + | Yes |
Chemistry : DDE
ORF sequence :
MPSKKHRPEEIIGKLREAEVVLAQGATTAEACRRIAVSEQTYYRWRKEYGGLKTDQARRMKDLEKENVRLRRAISDLTLDKLILQEAARGKLLSPARRRR
CIDHIRTMMPVSERRLCRVLGQYRSTQRKAPRGADDEAALTEDIIALARQYGRYGYRRVTALLRDAGWHVNRKRVERIWRREGLKVPQRQPKRGRLWLND
GSCIRLRPEYPGHVWSYDFVEGRTHDGRKYRILSIIDEASRECMALPVARKLKSDDVLAALAELFVTRGPPAHIRSDNGPEFIATAVQEWLAKVGVKTLC
IAPGSPWENGYCESFNGSLRDELLNGEIFYSLAEARILIEAWRRHYNTVRPHSSLGYRPPAPEAVPSPVSPSGSASLHLRPTLEMEASMH
CIDHIRTMMPVSERRLCRVLGQYRSTQRKAPRGADDEAALTEDIIALARQYGRYGYRRVTALLRDAGWHVNRKRVERIWRREGLKVPQRQPKRGRLWLND
GSCIRLRPEYPGHVWSYDFVEGRTHDGRKYRILSIIDEASRECMALPVARKLKSDDVLAALAELFVTRGPPAHIRSDNGPEFIATAVQEWLAKVGVKTLC
IAPGSPWENGYCESFNGSLRDELLNGEIFYSLAEARILIEAWRRHYNTVRPHSSLGYRPPAPEAVPSPVSPSGSASLHLRPTLEMEASMH
Blast result :
Comments
Genome in progress.
ISSpwi1 is 97% (ORFA) aa similar and 88% (ORFB) aa similar ISSpma1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISSpwi1 is 97% (ORFA) aa similar and 88% (ORFB) aa similar ISSpma1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct submission GenBank.