ISMaq1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008740 | ND | Marinobacter aquaeolei | Marinobacter aquaeolei VT8 |
DNA section
IS Length : 1194 bp
Ends
IR Length : 28/39
IRL : TGTAATCCCCCCAGAAAATAATCGAGGTTTTGAGTAGAGACTTTTACAAT
IRR : TGTAATCCCCCCATTTTTAACCGCTCATTTGAGTAGAGGTTAAGCTGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTGTTCAGTA | CCGA | CATGCTCAGG | 4 |
DNA sequence
TGTAATCCCCCCAGAAAATAATCGAGGTTTTGAGTAGAGACTTTTACAATGATCGCAAAAGGAGCGACAGATGAAGAAGTCTCGATTCACCGACAGCCAG
ATCATGGCGATCCTGAAGCAGCACGAAGCCGGCGTTCCGGTGTCTGAGCTGGCAAGGGAACACAATGTCAGTACGGCGCTGATCTATCAATGGCGCTCGA
AGTTTGGCGGCATGGATGCATCAATGATGAAACGCCTGAAGGAACTGGAAGCCGAGAATGCCCGCCTGAAGAAGATGTACGCCGAAGAGCGGCTTAAGGC
CGAGCTCCGTCAGGAAGCGCTGGAGGGAAAGTTGTAAGGCCATCTCAACGCCGGGAGATGGCCAGAAAGGGCGTGGCCAAACATGGCATCAGTATCCGTT
TGGCCTGCGTGGTGTATGGCATCAGCGAGACCTGCTATCGGTACCAGGCAAAGCTCAGTGGCGAGAACGCTCTGGTGGCTGACTGGCTATTGCGCCTTAC
CCAGACCCACAAACGCTGGGGCTTTGGTCTGTGCTTCCTGTATCTGCGCAATGTGAAGGGCTACACCTGGAACCATAAGCGCGTGTATCGGATTTATCGG
GAGCTGGAGCTGAACCTACGCATCAAGCCACGTCGACGAATCCAGCGGGATTATCCGGGTGAGCTGGATGTTCCGACGGCACCGAACCAGGTCTGGTCGA
TGGACTTCATGAGTGATCAACTGGTCAGCGGCAAGACATTCCGGACATTCAATGTGATCGACGACTACAACCGGGAAGGACTGGGCATCGAGGTCGACTT
GTCATTACCCGCACAGCGGGTGATTCGTGCGCTGGATCAGATCATTGAATGGCGCGGCAAGCCTGCCGCCATTCGCTGTGACAATGGGCCAGAGTATATC
TCACAGTCATTGATAGACTGGGCCAACAGAAACCGGATTACGCTGATCTACATTCAGCCCGGCAAGCCCACTCAGAATGCCTACATCGAGCGCTTCAATC
GAACCGCACGGCATGAATGGCTGGATTTGCACCAGTTCGAAAGCATTGCCCATGCTCAGCTTCTGGCAACTCAGTGGCTGTGGCAGTACAACAACGAACG
ACCGAACACGGCTATTGGAGGAGTTCCACCTTCCAGACTGCGGAAGGCAGCTTAACCTCTACTCAAATGAGCGGTTAAAAATGGGGGGATTACA
ATCATGGCGATCCTGAAGCAGCACGAAGCCGGCGTTCCGGTGTCTGAGCTGGCAAGGGAACACAATGTCAGTACGGCGCTGATCTATCAATGGCGCTCGA
AGTTTGGCGGCATGGATGCATCAATGATGAAACGCCTGAAGGAACTGGAAGCCGAGAATGCCCGCCTGAAGAAGATGTACGCCGAAGAGCGGCTTAAGGC
CGAGCTCCGTCAGGAAGCGCTGGAGGGAAAGTTGTAAGGCCATCTCAACGCCGGGAGATGGCCAGAAAGGGCGTGGCCAAACATGGCATCAGTATCCGTT
TGGCCTGCGTGGTGTATGGCATCAGCGAGACCTGCTATCGGTACCAGGCAAAGCTCAGTGGCGAGAACGCTCTGGTGGCTGACTGGCTATTGCGCCTTAC
CCAGACCCACAAACGCTGGGGCTTTGGTCTGTGCTTCCTGTATCTGCGCAATGTGAAGGGCTACACCTGGAACCATAAGCGCGTGTATCGGATTTATCGG
GAGCTGGAGCTGAACCTACGCATCAAGCCACGTCGACGAATCCAGCGGGATTATCCGGGTGAGCTGGATGTTCCGACGGCACCGAACCAGGTCTGGTCGA
TGGACTTCATGAGTGATCAACTGGTCAGCGGCAAGACATTCCGGACATTCAATGTGATCGACGACTACAACCGGGAAGGACTGGGCATCGAGGTCGACTT
GTCATTACCCGCACAGCGGGTGATTCGTGCGCTGGATCAGATCATTGAATGGCGCGGCAAGCCTGCCGCCATTCGCTGTGACAATGGGCCAGAGTATATC
TCACAGTCATTGATAGACTGGGCCAACAGAAACCGGATTACGCTGATCTACATTCAGCCCGGCAAGCCCACTCAGAATGCCTACATCGAGCGCTTCAATC
GAACCGCACGGCATGAATGGCTGGATTTGCACCAGTTCGAAAGCATTGCCCATGCTCAGCTTCTGGCAACTCAGTGGCTGTGGCAGTACAACAACGAACG
ACCGAACACGGCTATTGGAGGAGTTCCACCTTCCAGACTGCGGAAGGCAGCTTAACCTCTACTCAAATGAGCGGTTAAAAATGGGGGGATTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 71 | 337 | + | No |
Description : First part of the transposase
ORF sequence :
MKKSRFTDSQIMAILKQHEAGVPVSELAREHNVSTALIYQWRSKFGGMDASMMKRLKELEAENARLKKMYAEERLKAELRQEALEGKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 858 bp | 285 aa | 298 | 1155 | + | No |
Description : Second part of the transposase
ORF sequence :
GRAPSGSAGGKVVRPSQRREMARKGVAKHGISIRLACVVYGISETCYRYQAKLSGENALVADWLLRLTQTHKRWGFGLCFLYLRNVKGYTWNHKRVYRIY
RELELNLRIKPRRRIQRDYPGELDVPTAPNQVWSMDFMSDQLVSGKTFRTFNVIDDYNREGLGIEVDLSLPAQRVIRALDQIIEWRGKPAAIRCDNGPEY
ISQSLIDWANRNRITLIYIQPGKPTQNAYIERFNRTARHEWLDLHQFESIAHAQLLATQWLWQYNNERPNTAIGGVPPSRLRKAA
RELELNLRIKPRRRIQRDYPGELDVPTAPNQVWSMDFMSDQLVSGKTFRTFNVIDDYNREGLGIEVDLSLPAQRVIRALDQIIEWRGKPAAIRCDNGPEY
ISQSLIDWANRNRITLIYIQPGKPTQNAYIERFNRTARHEWLDLHQFESIAHAQLLATQWLWQYNNERPNTAIGGVPPSRLRKAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1085 bp | 361 aa | 71 | 1155 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKSRFTDSQIMAILKQHEAGVPVSELAREHNVSTALIYQWRSKFGGMDASMMKRLKELEAENARLKKMYAEERLKAELRQEALEGKVVRPSQRREMARK
GVAKHGISIRLACVVYGISETCYRYQAKLSGENALVADWLLRLTQTHKRWGFGLCFLYLRNVKGYTWNHKRVYRIYRELELNLRIKPRRRIQRDYPGELD
VPTAPNQVWSMDFMSDQLVSGKTFRTFNVIDDYNREGLGIEVDLSLPAQRVIRALDQIIEWRGKPAAIRCDNGPEYISQSLIDWANRNRITLIYIQPGKP
TQNAYIERFNRTARHEWLDLHQFESIAHAQLLATQWLWQYNNERPNTAIGGVPPSRLRKAA
GVAKHGISIRLACVVYGISETCYRYQAKLSGENALVADWLLRLTQTHKRWGFGLCFLYLRNVKGYTWNHKRVYRIYRELELNLRIKPRRRIQRDYPGELD
VPTAPNQVWSMDFMSDQLVSGKTFRTFNVIDDYNREGLGIEVDLSLPAQRVIRALDQIIEWRGKPAAIRCDNGPEYISQSLIDWANRNRITLIYIQPGKP
TQNAYIERFNRTARHEWLDLHQFESIAHAQLLATQWLWQYNNERPNTAIGGVPPSRLRKAA
Blast result :
Comments
ISMaq1 is 86 % (ORFA) aa similar to ISSod2 and 79% (ORFB) aa similar to ISXac2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Edwards,K. and Richardson,P. (2007) Direct submission GenBank.