ISAma2
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAOD01000014 | ND | Alteromonas macleodii | Alteromonas macleodii |
DNA section
IS Length : 1192 bp
Ends
IR Length : 35/46
IRL : TGTCCACTTCCCGTAAACTGAGACAGACAAAATTAGAGTTTTCTGTAATT
IRR : TGTCCACTTCCCCATTAATGACACAACTCTAAGTTAGAGTTTTCCAGTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGGTCAAAAT | ACTC | TACTGGGATA | 4 |
DNA sequence
TGTCCACTTCCCGTAAACTGAGACAGACAAAATTAGAGTTTTCTGTAATTATTAATGCAGGAGACGACTATGAAAAAATCACGCTATACAGAATCTCAGA
TCATCGGAGTCTTAAAAGAAGTCGATGCTGGTAGAAAAGTTGAAGAAGTGTGCCGCCAACACGGTATTAGCAGTGCCACTTATTACAACTGGAAGTCCAA
ATACGGTGGCATGGAAGCGTCTGACGTTAAAAGACTGAAAGAGCTTGAAGAAGAAAATGCCAAGCTAAAGAAGATGTTCGCTGACGTTAGCCTAGAAGAT
AATGCGATTAAGGAGCTTGTCGCAAAAAAGGGTTGGTGACAGCAGAGAAGCGAGAGTGCGCCAGCATACTAGTTGATGCGGGGCTGAGTATCGTAAAGGC
TTGCCTATTTGTTGGTATTAGCCGTTCGACCTTCTACCGCCCCGAACGAGACTGGCGCAAAGCAGATGCTGCTGTCATTGATGCTATCAATGCTGTGCTT
GATAAGTCGCCACGAGCAGGCTTCTGGAAGTGCTTTGGCCGAATGCGTTTCAAAGGCTTTCCGTTCAACCATAAGCGCGTTTATCGCGTGTATTGTCAAA
TGGGATTGAATTTAAGGCGCAGAACCAAACGTGTGCTCCCTAAACGAATAGCACAGCCGCTGGAAGTCTTGGAGCAAGCGAACTACCAATGGGCGTTGGA
CTTTATGCATGACACTTTATACTGCGGTAAGCGGTTCAGAACATTAAATGTGGTGGATGAAGGAACACGGGAATGCTTAGCCATTGAAGTAGACACCTCA
TTACCTGCTGGCCGCGTAGTACGCGTATTAGAACAGCTTAAAACTGAACGCGGATTGCCTAAGCAACTGCGCATGGATAATGGCCCCGAGCTAATTTCAG
CGACGCTAACAGATTGGTGTCAGAACCATAATATTGAACTGTTATATATTCAGCCAGGCAAGCCACAGCAAAATGGCTTTGTAGAACGCTTTAATGGTTC
ATTCCGCAGAGAGTTTCTCGATGCCTACTTGTTTGAGAATATCGGCCAGGTGAGAGAAATGTCTTGGTTCTGGCGACTAGATTATAACGAAGAGAGAACT
CATGAAAGTCTCGGAAATCTGCCTCCGGCAGCTTACTGAGAAAAACTGGAAAACTCTAACTTAGAGTTGTGTCATTAATGGGGAAGTGGACA
TCATCGGAGTCTTAAAAGAAGTCGATGCTGGTAGAAAAGTTGAAGAAGTGTGCCGCCAACACGGTATTAGCAGTGCCACTTATTACAACTGGAAGTCCAA
ATACGGTGGCATGGAAGCGTCTGACGTTAAAAGACTGAAAGAGCTTGAAGAAGAAAATGCCAAGCTAAAGAAGATGTTCGCTGACGTTAGCCTAGAAGAT
AATGCGATTAAGGAGCTTGTCGCAAAAAAGGGTTGGTGACAGCAGAGAAGCGAGAGTGCGCCAGCATACTAGTTGATGCGGGGCTGAGTATCGTAAAGGC
TTGCCTATTTGTTGGTATTAGCCGTTCGACCTTCTACCGCCCCGAACGAGACTGGCGCAAAGCAGATGCTGCTGTCATTGATGCTATCAATGCTGTGCTT
GATAAGTCGCCACGAGCAGGCTTCTGGAAGTGCTTTGGCCGAATGCGTTTCAAAGGCTTTCCGTTCAACCATAAGCGCGTTTATCGCGTGTATTGTCAAA
TGGGATTGAATTTAAGGCGCAGAACCAAACGTGTGCTCCCTAAACGAATAGCACAGCCGCTGGAAGTCTTGGAGCAAGCGAACTACCAATGGGCGTTGGA
CTTTATGCATGACACTTTATACTGCGGTAAGCGGTTCAGAACATTAAATGTGGTGGATGAAGGAACACGGGAATGCTTAGCCATTGAAGTAGACACCTCA
TTACCTGCTGGCCGCGTAGTACGCGTATTAGAACAGCTTAAAACTGAACGCGGATTGCCTAAGCAACTGCGCATGGATAATGGCCCCGAGCTAATTTCAG
CGACGCTAACAGATTGGTGTCAGAACCATAATATTGAACTGTTATATATTCAGCCAGGCAAGCCACAGCAAAATGGCTTTGTAGAACGCTTTAATGGTTC
ATTCCGCAGAGAGTTTCTCGATGCCTACTTGTTTGAGAATATCGGCCAGGTGAGAGAAATGTCTTGGTTCTGGCGACTAGATTATAACGAAGAGAGAACT
CATGAAAGTCTCGGAAATCTGCCTCCGGCAGCTTACTGAGAAAAACTGGAAAACTCTAACTTAGAGTTGTGTCATTAATGGGGAAGTGGACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 270 bp | 89 aa | 70 | 339 | + | No |
Description : First part of the transposase
ORF sequence :
MKKSRYTESQIIGVLKEVDAGRKVEEVCRQHGISSATYYNWKSKYGGMEASDVKRLKELEEENAKLKKMFADVSLEDNAIKELVAKKGW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 828 bp | 275 aa | 312 | 1139 | + | No |
Description : Second part of the transposase
ORF sequence :
GACRKKGLVTAEKRECASILVDAGLSIVKACLFVGISRSTFYRPERDWRKADAAVIDAINAVLDKSPRAGFWKCFGRMRFKGFPFNHKRVYRVYCQMGLN
LRRRTKRVLPKRIAQPLEVLEQANYQWALDFMHDTLYCGKRFRTLNVVDEGTRECLAIEVDTSLPAGRVVRVLEQLKTERGLPKQLRMDNGPELISATLT
DWCQNHNIELLYIQPGKPQQNGFVERFNGSFRREFLDAYLFENIGQVREMSWFWRLDYNEERTHESLGNLPPAAY
LRRRTKRVLPKRIAQPLEVLEQANYQWALDFMHDTLYCGKRFRTLNVVDEGTRECLAIEVDTSLPAGRVVRVLEQLKTERGLPKQLRMDNGPELISATLT
DWCQNHNIELLYIQPGKPQQNGFVERFNGSFRREFLDAYLFENIGQVREMSWFWRLDYNEERTHESLGNLPPAAY
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1070 bp | 356 aa | 70 | 1139 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKSRYTESQIIGVLKEVDAGRKVEEVCRQHGISSATYYNWKSKYGGMEASDVKRLKELEEENAKLKKMFADVSLEDNAIKELVAKKGLVTAEKRECASI
LVDAGLSIVKACLFVGISRSTFYRPERDWRKADAAVIDAINAVLDKSPRAGFWKCFGRMRFKGFPFNHKRVYRVYCQMGLNLRRRTKRVLPKRIAQPLEV
LEQANYQWALDFMHDTLYCGKRFRTLNVVDEGTRECLAIEVDTSLPAGRVVRVLEQLKTERGLPKQLRMDNGPELISATLTDWCQNHNIELLYIQPGKPQ
QNGFVERFNGSFRREFLDAYLFENIGQVREMSWFWRLDYNEERTHESLGNLPPAAY
LVDAGLSIVKACLFVGISRSTFYRPERDWRKADAAVIDAINAVLDKSPRAGFWKCFGRMRFKGFPFNHKRVYRVYCQMGLNLRRRTKRVLPKRIAQPLEV
LEQANYQWALDFMHDTLYCGKRFRTLNVVDEGTRECLAIEVDTSLPAGRVVRVLEQLKTERGLPKQLRMDNGPELISATLTDWCQNHNIELLYIQPGKPQ
QNGFVERFNGSFRREFLDAYLFENIGQVREMSWFWRLDYNEERTHESLGNLPPAAY
Blast result :
Comments
ISAma2 is 91% (ORFA) and 70% (ORFB) aa similar to ISD1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Rodriguez-Valera,F., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct Submission GenBank.