ISEli1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007722 | ND | Erythrobacter litoralis | Erythrobacter litoralis HTCC2594 |
DNA section
IS Length : 1270 bp
Ends
IR Length : 37/46
IRL : TGACGTGACCCCCTGATTTTCCTCCAAGTTGGATTAGAGTCCGGCCCTGA
IRR : TGACGTGACCCCCAGCTTTCCCCCAGCTGGGATTAGAGCCGGGCGGTTGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGTTGGGAC | ATAG | ATAACGAGCA | 4 |
DNA sequence
TGACGTGACCCCCTGATTTTCCTCCAAGTTGGATTAGAGTCCGGCCCTGACAGAAGGACGGACGAGATGAAGAGAACGAGGTTTTCAGAAGAGCAGATCA
TCGGCGTGCTCAAGGAGGCGGAGGCGGGAGCGAAGACCGCCGACCTGGCCCGGCGACACGGGGTTTCGGAAGCGACGATCTACAACTGGAAGTCGAAGTA
TGGTGGGCTGGAGGTGTCTGATGCCCGGCGGCTCAAGGAGCTCGAGAGCGAGAACGCCAAGCTCAAGCGCCTGCTGGCCGACACGATGCTGGACAAAGCG
GCGTTGAAGGATCTTCTGGCAAAAAAGTTCTGACGCCCGCCGCGCAGCGGGAAGCTGTCGCTCATCTTCAGGCATGCCACGGGATGAGCGAGCGGCGGGC
GTGCCGTGTCATCGATGCCGATCGCAAGAGCGTGCGTTACCGTTCCACCCGGGGCGATGACGCCGATCTGCGTGAGAAGCTGCGCGAGCTGGCCAACCAG
CGACGGCGGTTCGGCTATCGCCGTCTGCACATCCTGCTGCGCCGGGAGGGGATCATGATCAACCGGAAGAAGACCCAGAGGCTCTACAAGGAGGAAGGCT
TGGCGGTGAGGCGTCGACGCAGTCGCAGGCGCGCAGTCGGCACGAGGGCACCTGCTCCGGTGCTCGCCTTACCGAACCAGCGCTGGAGCCTGGACTTCGT
GCACGACCAGATGGCGTCGGGCAGGAGGTTCCGGGTGCTCAACGTGGTCGATGACGTGACAAGGGAGTGTCTGGCAGCGGTGCCGGACACGTCGATCTCT
GGTCGCCGTGTCGTTCGAGAGCTGACCGAGCTGATCGCCCAGCGTGGCAAGCCCGGCATGATCGTCAGCGACAACGGAACCGAGCTCACCAGCAACGCCG
TGCTGGCATGGTGCGGCGAGATCGGCGTCGAGTGGCATTATATCGCGCCCGGAAGGCCGATGCAGAATGGCTATGTCGAGAGCTTCAACGGTCGCATGCG
GGACGAACTGCTCAACGAGACGCTGTTCATGAGCATGGCCCATGCCCGTGTCGAGATCGCCGCCTGGGTGGATGATTACAACCGGGAGCGACCGCACTCA
TCGCTTGGCTACGCAACACCGGCGGCGTTCGCCGCCGAACTGGATAAGCAATGGCCTGCTTCGCTACGCCCTACGGGCTCCACTACGCAGCCCATTGCTT
CAATCGCGCTTATGCGCAAAACAACCGCCCGGCTCTAATCCCAGCTGGGGGAAAGCTGGGGGTCACGTCA
TCGGCGTGCTCAAGGAGGCGGAGGCGGGAGCGAAGACCGCCGACCTGGCCCGGCGACACGGGGTTTCGGAAGCGACGATCTACAACTGGAAGTCGAAGTA
TGGTGGGCTGGAGGTGTCTGATGCCCGGCGGCTCAAGGAGCTCGAGAGCGAGAACGCCAAGCTCAAGCGCCTGCTGGCCGACACGATGCTGGACAAAGCG
GCGTTGAAGGATCTTCTGGCAAAAAAGTTCTGACGCCCGCCGCGCAGCGGGAAGCTGTCGCTCATCTTCAGGCATGCCACGGGATGAGCGAGCGGCGGGC
GTGCCGTGTCATCGATGCCGATCGCAAGAGCGTGCGTTACCGTTCCACCCGGGGCGATGACGCCGATCTGCGTGAGAAGCTGCGCGAGCTGGCCAACCAG
CGACGGCGGTTCGGCTATCGCCGTCTGCACATCCTGCTGCGCCGGGAGGGGATCATGATCAACCGGAAGAAGACCCAGAGGCTCTACAAGGAGGAAGGCT
TGGCGGTGAGGCGTCGACGCAGTCGCAGGCGCGCAGTCGGCACGAGGGCACCTGCTCCGGTGCTCGCCTTACCGAACCAGCGCTGGAGCCTGGACTTCGT
GCACGACCAGATGGCGTCGGGCAGGAGGTTCCGGGTGCTCAACGTGGTCGATGACGTGACAAGGGAGTGTCTGGCAGCGGTGCCGGACACGTCGATCTCT
GGTCGCCGTGTCGTTCGAGAGCTGACCGAGCTGATCGCCCAGCGTGGCAAGCCCGGCATGATCGTCAGCGACAACGGAACCGAGCTCACCAGCAACGCCG
TGCTGGCATGGTGCGGCGAGATCGGCGTCGAGTGGCATTATATCGCGCCCGGAAGGCCGATGCAGAATGGCTATGTCGAGAGCTTCAACGGTCGCATGCG
GGACGAACTGCTCAACGAGACGCTGTTCATGAGCATGGCCCATGCCCGTGTCGAGATCGCCGCCTGGGTGGATGATTACAACCGGGAGCGACCGCACTCA
TCGCTTGGCTACGCAACACCGGCGGCGTTCGCCGCCGAACTGGATAAGCAATGGCCTGCTTCGCTACGCCCTACGGGCTCCACTACGCAGCCCATTGCTT
CAATCGCGCTTATGCGCAAAACAACCGCCCGGCTCTAATCCCAGCTGGGGGAAAGCTGGGGGTCACGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 67 | 333 | + | No |
Description : First part of the transposase
ORF sequence :
MKRTRFSEEQIIGVLKEAEAGAKTADLARRHGVSEATIYNWKSKYGGLEVSDARRLKELESENAKLKRLLADTMLDKAALKDLLAKKF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1017 bp | 338 aa | 222 | 1238 | + | No |
Description : Second part of the transposase
ORF sequence :
CPAAQGARERERQAQAPAGRHDAGQSGVEGSSGKKVLTPAAQREAVAHLQACHGMSERRACRVIDADRKSVRYRSTRGDDADLREKLRELANQRRRFGYR
RLHILLRREGIMINRKKTQRLYKEEGLAVRRRRSRRRAVGTRAPAPVLALPNQRWSLDFVHDQMASGRRFRVLNVVDDVTRECLAAVPDTSISGRRVVRE
LTELIAQRGKPGMIVSDNGTELTSNAVLAWCGEIGVEWHYIAPGRPMQNGYVESFNGRMRDELLNETLFMSMAHARVEIAAWVDDYNRERPHSSLGYATP
AAFAAELDKQWPASLRPTGSTTQPIASIALMRKTTARL
RLHILLRREGIMINRKKTQRLYKEEGLAVRRRRSRRRAVGTRAPAPVLALPNQRWSLDFVHDQMASGRRFRVLNVVDDVTRECLAAVPDTSISGRRVVRE
LTELIAQRGKPGMIVSDNGTELTSNAVLAWCGEIGVEWHYIAPGRPMQNGYVESFNGRMRDELLNETLFMSMAHARVEIAAWVDDYNRERPHSSLGYATP
AAFAAELDKQWPASLRPTGSTTQPIASIALMRKTTARL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1172 bp | 390 aa | 67 | 1238 | + | Yes |
Chemistry : DDE
ORF sequence :
MKRTRFSEEQIIGVLKEAEAGAKTADLARRHGVSEATIYNWKSKYGGLEVSDARRLKELESENAKLKRLLADTMLDKAALKDLLAKKVLTPAAQREAVAH
LQACHGMSERRACRVIDADRKSVRYRSTRGDDADLREKLRELANQRRRFGYRRLHILLRREGIMINRKKTQRLYKEEGLAVRRRRSRRRAVGTRAPAPVL
ALPNQRWSLDFVHDQMASGRRFRVLNVVDDVTRECLAAVPDTSISGRRVVRELTELIAQRGKPGMIVSDNGTELTSNAVLAWCGEIGVEWHYIAPGRPMQ
NGYVESFNGRMRDELLNETLFMSMAHARVEIAAWVDDYNRERPHSSLGYATPAAFAAELDKQWPASLRPTGSTTQPIASIALMRKTTARL
LQACHGMSERRACRVIDADRKSVRYRSTRGDDADLREKLRELANQRRRFGYRRLHILLRREGIMINRKKTQRLYKEEGLAVRRRRSRRRAVGTRAPAPVL
ALPNQRWSLDFVHDQMASGRRFRVLNVVDDVTRECLAAVPDTSISGRRVVRELTELIAQRGKPGMIVSDNGTELTSNAVLAWCGEIGVEWHYIAPGRPMQ
NGYVESFNGRMRDELLNETLFMSMAHARVEIAAWVDDYNRERPHSSLGYATPAAFAAELDKQWPASLRPTGSTTQPIASIALMRKTTARL
Blast result :
Comments
ISEli1 is 86 % (ORFA) aa similar to ISRpa2 and 81% (ORFB) aa similar to ISOba1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Giovannoni,S.J., Cho,J.-C., Ferriera,S., Johnson,J., Kravits,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2005) Direct submission GenBank.