ISArsp1

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008541 ND Arthrobacter sp.
Arthrobacter sp. FB24 plasmid 1
Arthrobacter sp. FB24
DNA section
IS Length : 1077 bp

Ends


IR Length : 25

IRL : TGTAATTCCCACGGAGGTTGTGAACAGCGTGGCGTGATGTAAAAAGCGAG
IRR : TGTAATTCCCACGGAGGTTGTGAACGCGGCTGATGGGAGTTTTTCCTCCG

Insertion site


Left flankDirect repeatRight flankDR Length
CTCCTCCGGCTCTAGGGCTCACTGAA6
ATATCCGTGCGCTAGCGCACGGATAT6

DNA sequence

TGTAATTCCCACGGAGGTTGTGAACAGCGTGGCGTGATGTAAAAAGCGAGAGCCTCCGGTATCGATTTGGGTTACCACACTCATCTCGACGCCAGGAGGC
TCTCGTGTCCTACGTTACTCATGCCAACGCCGACCTGACACCCAAAGCCAGGGGAAAGCTCGCCCGGCTGGTCATCGAGCAGGGCTGGACTCTACGCCGG
GCCGCGGAACGGTTCCAGTGCTCACCGGCCACCGCGAAGAAATGGGTGGACCGGTACCGGGCCCGTGGCGAGGACGGCATGGCTGACTTGTCCTCGCGGC
CGCGTCGAAGCCCGAACCGCACTGATGTCCGCACCGAACGGCGGATTCTGGCGCTGCGTTTCACCCGCCGGTGGGGTCCTCACCGGATCGCAGCCCACCT
GCACCTTGCCCGCTCGACCGTGGGAAAGGTCCTGACCCGGTACCGGATGCCCCGGCTGGCCTGCCTGGACCAAGGCACCGGCCTGCCCATCCGCAAGCCG
GCACCCCAACGCTACGAACACGACCATCCCGGGGACCTGGTACACGTGGACATCAAGAAACTCGGACGCATCCCAGACGGCGGCGGGCACCGCGCCCTGG
GACGGGCGGCAGGCCGTAAGAACCGCCGGGCCGGGACCGGCTACGCCTACCTGCACCACGCCGTGGATGACCATTCCCGCCTGGCCTACTCCGAAATACT
CACCGACGAAAAGAAGGAAACCGCCACAGCGTTCTGGTTCCGCGCCGCATCGTTCTTCGCCGCCCACGGCATCACTGTCAGAGCCGTGCTCACCGATAAC
GGCGCTTGCTACCGCTCCAGAGCCTTCACAGCCGCCCTGGGCCCGAACATCAAGCACCGCAGGACTCGGCCCTACCGGCCACAGACCAATGGCAAGGTAG
AACGCTTCAACCGGACCCTCAACACGGAATGGGCCTACGCCCGGCCCTACACATCAGAAGCCGAGCGAGCAGCCACCTACCCCGGTTGGCTACATCAGTA
CAATCATCACAGAACTCATACCGGCATCGGAGGAAAAACTCCCATCAGCCGCGTTCACAACCTCCGTGGGAATTACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
978 bp325 aa1051082+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VSYVTHANADLTPKARGKLARLVIEQGWTLRRAAERFQCSPATAKKWVDRYRARGEDGMADLSSRPRRSPNRTDVRTERRILALRFTRRWGPHRIAAHLH
LARSTVGKVLTRYRMPRLACLDQGTGLPIRKPAPQRYEHDHPGDLVHVDIKKLGRIPDGGGHRALGRAAGRKNRRAGTGYAYLHHAVDDHSRLAYSEILT
DEKKETATAFWFRAASFFAAHGITVRAVLTDNGACYRSRAFTAALGPNIKHRRTRPYRPQTNGKVERFNRTLNTEWAYARPYTSEAERAATYPGWLHQYN
HHRTHTGIGGKTPISRVHNLRGNYI

 

Blast result :
Comments
ISArsp1 is 79% aa similar to ISMyma6.
The stop codon is in the insertion site.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Thompson,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Beasley,F., Chen,W., Jerke,K., Nakatsu,C.H. and Richardson,P.US DOE Joint Genome Institute. (2006) Direct submission GenBank.