ISSpr2
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAUN01000023 | ND | Serratia proteamaculans | Serratia proteamaculans 568 |
DNA section
IS Length : 1221 bp
Ends
IR Length : 36/46
IRL : TGACCTGCTCCCCGTTGATTAGTACACCCCGATGTTAGTAATGTCTTCAT
IRR : TGACCTGCCCCCAGGATTAGATACAACCTTCAGTTAGTAATGTCGGTTGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GACGCTCAGT | ATGAACCCAT | 0 |
DNA sequence
TGACCTGCTCCCCGTTGATTAGTACACCCCGATGTTAGTAATGTCTTCATCTGCAACGTGAGGACATCCCCATGAAGAAGCGTTTTTCCGACCAACAGAT
CATCAGTATTCTCCGCGAGGCTGAAGCCGGGGTATCTGCCCGTGAACTCTGCCGCAAGCACGCCATTTCCGATGCCACTTTTTACACCTGGCGTAAGAAG
TATGGCGGCATGGAAGTGCCTGAGGTTAAGCGCCTGAAGTCGCTTGAAGAAGAGAACGCCCGACTCAAGAAGCTGCTCGCCGAAGCCATGCTCGATAAGG
AGGCGCTTCAGGTGGCTCTTGGGCGAAAGTACTGACGACAGACCAGAAGCGGGAAGCCGTGGAGTTGATGTGTGGTGCGACCGGTCTGTCGCAACGTCGT
GCCTGCAGGCTGACAGGTTTGTCCCTGTCGACCTGCCGCTATGAAGCGCAGCGTCCGACGTCGGATGCGCATTTATCAGGGCGCATCACTGAACTGGCGC
TTGAACGCAGGCGTTTTGGTTACCGGCGCATCTGGCAGTTACTGCGTCGGGAGGGCGTTCACGTCAATCACAAGCGGGTATACCGCATTTACCATCTTAA
CAGCCTGAGCGTAAAACGCAGACGACGTCGTAAAGGGCTGGCGACCGAGCGGTTTCCCCTTCTGCGCCCAGATGCGCCGAACCTGACCTGGTCGATGGAT
TTCGTCATGGACGCACTGGCCACCGGTCGCAGGATCAAGTGTCTGACCTGCGTGGATGATTTCACGAAAGAGTGCCTGACGATCACCGCCGCTTTCGGGA
TTTCAGGCGTGCAGGTCATGCGTATTCTGGACAGCATTGCGCTGTTTCGCGGCTATCCGGCGACGATAAGAACCGATCAGGGGCCGGAATTTACCTGCCG
CGCATTGGAGCAATGGGCCTTTGAGCATGGCGTGGAGCTGCGGCTTATCCAGCCGGGCAAGCCGACCCAGAACGGATTTATTGAGAGTTTTAACGGACGC
TTTCGCGATGAATGCCTGAATGAGCACTGGTTCAGCGATATTGTTCACGCCAGGAAAATCATTAATAACTGGCGACAAGATTATAACGAGTGCTGTCCTC
ATTCATCGCTGAACTACCAGACTCCAGCTGAATTTGCAGCTGGCTGGCGAAACAGGAAATATGAAGAAAAACCAACCGACATTACTAACTGAAGGTTGTA
TCTAATCCTGGGGGCAGGTCA
CATCAGTATTCTCCGCGAGGCTGAAGCCGGGGTATCTGCCCGTGAACTCTGCCGCAAGCACGCCATTTCCGATGCCACTTTTTACACCTGGCGTAAGAAG
TATGGCGGCATGGAAGTGCCTGAGGTTAAGCGCCTGAAGTCGCTTGAAGAAGAGAACGCCCGACTCAAGAAGCTGCTCGCCGAAGCCATGCTCGATAAGG
AGGCGCTTCAGGTGGCTCTTGGGCGAAAGTACTGACGACAGACCAGAAGCGGGAAGCCGTGGAGTTGATGTGTGGTGCGACCGGTCTGTCGCAACGTCGT
GCCTGCAGGCTGACAGGTTTGTCCCTGTCGACCTGCCGCTATGAAGCGCAGCGTCCGACGTCGGATGCGCATTTATCAGGGCGCATCACTGAACTGGCGC
TTGAACGCAGGCGTTTTGGTTACCGGCGCATCTGGCAGTTACTGCGTCGGGAGGGCGTTCACGTCAATCACAAGCGGGTATACCGCATTTACCATCTTAA
CAGCCTGAGCGTAAAACGCAGACGACGTCGTAAAGGGCTGGCGACCGAGCGGTTTCCCCTTCTGCGCCCAGATGCGCCGAACCTGACCTGGTCGATGGAT
TTCGTCATGGACGCACTGGCCACCGGTCGCAGGATCAAGTGTCTGACCTGCGTGGATGATTTCACGAAAGAGTGCCTGACGATCACCGCCGCTTTCGGGA
TTTCAGGCGTGCAGGTCATGCGTATTCTGGACAGCATTGCGCTGTTTCGCGGCTATCCGGCGACGATAAGAACCGATCAGGGGCCGGAATTTACCTGCCG
CGCATTGGAGCAATGGGCCTTTGAGCATGGCGTGGAGCTGCGGCTTATCCAGCCGGGCAAGCCGACCCAGAACGGATTTATTGAGAGTTTTAACGGACGC
TTTCGCGATGAATGCCTGAATGAGCACTGGTTCAGCGATATTGTTCACGCCAGGAAAATCATTAATAACTGGCGACAAGATTATAACGAGTGCTGTCCTC
ATTCATCGCTGAACTACCAGACTCCAGCTGAATTTGCAGCTGGCTGGCGAAACAGGAAATATGAAGAAAAACCAACCGACATTACTAACTGAAGGTTGTA
TCTAATCCTGGGGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 264 bp | 87 aa | 72 | 335 | + | No |
Description : First part of the transposase
ORF sequence :
MCGATGLSQRRACRLTGLSLSTCRYEAQRPTSDAHLSGRITELALERRRFGYRRIWQLLRREGVHVNHKRVYRIYHLNSLSVKRRRRRKGLATERFPLLR
PDAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTITAAFGISGVQVMRILDSIALFRGYPATIRTDQGPEFTCRALEQWAFEHGVELRLIQPGKPT
QNGFIESFNGRFRDECLNEHWFSDIVHARKIINNWRQDYNECCPHSSLNYQTPAEFAAGWRNRKYEEKPTDITN
PDAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTITAAFGISGVQVMRILDSIALFRGYPATIRTDQGPEFTCRALEQWAFEHGVELRLIQPGKPT
QNGFIESFNGRFRDECLNEHWFSDIVHARKIINNWRQDYNECCPHSSLNYQTPAEFAAGWRNRKYEEKPTDITN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 894 bp | 297 aa | 299 | 1192 | + | No |
Description : Second part of the transposase
ORF sequence :
GGASGGSWAKVLTTDQKREAVELMCGATGLSQRRACRLTGLSLSTCRYEAQRPTSDAHLSGRITELALERRRFGYRRIWQLLRREGVHVNHKRVYRIYHL
NSLSVKRRRRRKGLATERFPLLRPDAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTITAAFGISGVQVMRILDSIALFRGYPATIRTDQGPEFTC
RALEQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINNWRQDYNECCPHSSLNYQTPAEFAAGWRNRKYEEKPTDITN
NSLSVKRRRRRKGLATERFPLLRPDAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTITAAFGISGVQVMRILDSIALFRGYPATIRTDQGPEFTC
RALEQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINNWRQDYNECCPHSSLNYQTPAEFAAGWRNRKYEEKPTDITN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1121 bp | 373 aa | 72 | 1192 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRFSDQQIISILREAEAGVSARELCRKHAISDATFYTWRKKYGGMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKVLTTDQKREAVELM
CGATGLSQRRACRLTGLSLSTCRYEAQRPTSDAHLSGRITELALERRRFGYRRIWQLLRREGVHVNHKRVYRIYHLNSLSVKRRRRRKGLATERFPLLRP
DAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTITAAFGISGVQVMRILDSIALFRGYPATIRTDQGPEFTCRALEQWAFEHGVELRLIQPGKPTQ
NGFIESFNGRFRDECLNEHWFSDIVHARKIINNWRQDYNECCPHSSLNYQTPAEFAAGWRNRKYEEKPTDITN
CGATGLSQRRACRLTGLSLSTCRYEAQRPTSDAHLSGRITELALERRRFGYRRIWQLLRREGVHVNHKRVYRIYHLNSLSVKRRRRRKGLATERFPLLRP
DAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTITAAFGISGVQVMRILDSIALFRGYPATIRTDQGPEFTCRALEQWAFEHGVELRLIQPGKPTQ
NGFIESFNGRFRDECLNEHWFSDIVHARKIINNWRQDYNECCPHSSLNYQTPAEFAAGWRNRKYEEKPTDITN
Blast result :
Comments
Genome in progress.
ISSpr2 is 100% (ORFA) and 91% (ORFB) aa similar to IS1222. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISSpr2 is 100% (ORFA) and 91% (ORFB) aa similar to IS1222. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct submission GenBank.