ISMamg1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007626 | ND | Magnetospirillum magneticum | Magnetospirillum magneticum AMB-1 |
DNA section
IS Length : 1256 bp
Ends
IR Length : 37/47
IRL : TGACCTGTTCCCCTCGAATGTAGCCAGTTTGAGGTAGAGTCCGTCCCATC
IRR : TGACCTGTTCCCCTGATTGATCCCAGTCAGAGGTAGAGCCCCGTCTCCCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGGCCGCTCC | GGGAACAGGT | 0 |
DNA sequence
TGACCTGTTCCCCTCGAATGTAGCCAGTTTGAGGTAGAGTCCGTCCCATCAAGGAGACGGAACGATGAAGCGTAGCCGGTTCAGCGAGGAGCAGATCATC
GGCATCCTGAAGGAGCAGGAGGCCGGTCAGGCGACAGCGGATGTGTGCCGCAGGCACGGCATATCGAGCGCAACCTTCTACAAATGGAAGGCCAAGTACG
GCGGGCTGGAGGTGTCGGAGGCCAAGCGGCTAAAGGCGCTTGAGGACGAAAACGCTCGGCTGAAGAAACTGCTGGCCGAAGCCATGCTCGACAACGCCGT
GCTGAAGGAAATTTCCTCAAAAAAATGGTGACGCCCGCCGCCAGGCGGATCGTCGTCGCCCATGTCCGAGAATTGTTCGGACTGAGCGAGCGTCGGGCGT
GTTTGATTGTGGGGATTTCCCGGCGAGTGATGCGGTATCGGTCGTGTCGTCCCGACGACGCGCCGATCCGGGCGCGATTGCGCGAACTGGCGGCAGAGCG
TCGCCGGTTCGGCTTCCGACGTCTGGGCATCCTGCTATCCAGGGAAGGCGTGGCGATGAACCGTAAGAAGCTGCTGCGGCTGTACCGCGAGGAGAAGCTG
GCGGTGCGCCCAAGGCGTGGCCGCAAGCGAGCCCTGGGTTGCCGGGCCCCAATGGCGATCCCGCAGGGGCCGAACCAATACTGGTCGCTGGATTTTCTGT
CCGACGCCTTCACCGACGGCCGCAGGTTCCGCATCTTTGCCGTGGTGGATGATTTTACCCGTGAGTGCTTGGCGCTGGTCGCCGACACTTCGCTGTCCGG
TGTCCGTGTCGCCCGGGAGTTGGATGGTCTGATCGCCGGGCGCGGCAAGCCGCAGATGATCGTCAGCGACAACGGCACCGAGTTCACCAGCATGGCCATG
CTGCGCTGGAGCCAGGATCACCGGGTGGAATGGCATTACATCGCACCGGGCAAGCCCATGCAGAACGGCTTCGTCGAGAGCTTCAACGGCAAGCTACGCG
ATGAATGCCTCAACGAGACGCTGTTTGGCTCGATCAATCATGCTCGGGCCGTCCTGGCGGACTGGAAGGAAGATTACAGCCATGTGCGGCCCCACACCGC
CTTGGGCGGCATCGCCCCAGCGCAGGCCGCCGCACGAGCGCGAGCGCAATGTGGGCCGGGGCATGCCCCGGCCCACATTGCCATCACCGCCCATTTCGGG
CATCACGGGGAGACGGGGCTCTACCTCTGACTGGGATCAATCAGGGGAACAGGTCA
GGCATCCTGAAGGAGCAGGAGGCCGGTCAGGCGACAGCGGATGTGTGCCGCAGGCACGGCATATCGAGCGCAACCTTCTACAAATGGAAGGCCAAGTACG
GCGGGCTGGAGGTGTCGGAGGCCAAGCGGCTAAAGGCGCTTGAGGACGAAAACGCTCGGCTGAAGAAACTGCTGGCCGAAGCCATGCTCGACAACGCCGT
GCTGAAGGAAATTTCCTCAAAAAAATGGTGACGCCCGCCGCCAGGCGGATCGTCGTCGCCCATGTCCGAGAATTGTTCGGACTGAGCGAGCGTCGGGCGT
GTTTGATTGTGGGGATTTCCCGGCGAGTGATGCGGTATCGGTCGTGTCGTCCCGACGACGCGCCGATCCGGGCGCGATTGCGCGAACTGGCGGCAGAGCG
TCGCCGGTTCGGCTTCCGACGTCTGGGCATCCTGCTATCCAGGGAAGGCGTGGCGATGAACCGTAAGAAGCTGCTGCGGCTGTACCGCGAGGAGAAGCTG
GCGGTGCGCCCAAGGCGTGGCCGCAAGCGAGCCCTGGGTTGCCGGGCCCCAATGGCGATCCCGCAGGGGCCGAACCAATACTGGTCGCTGGATTTTCTGT
CCGACGCCTTCACCGACGGCCGCAGGTTCCGCATCTTTGCCGTGGTGGATGATTTTACCCGTGAGTGCTTGGCGCTGGTCGCCGACACTTCGCTGTCCGG
TGTCCGTGTCGCCCGGGAGTTGGATGGTCTGATCGCCGGGCGCGGCAAGCCGCAGATGATCGTCAGCGACAACGGCACCGAGTTCACCAGCATGGCCATG
CTGCGCTGGAGCCAGGATCACCGGGTGGAATGGCATTACATCGCACCGGGCAAGCCCATGCAGAACGGCTTCGTCGAGAGCTTCAACGGCAAGCTACGCG
ATGAATGCCTCAACGAGACGCTGTTTGGCTCGATCAATCATGCTCGGGCCGTCCTGGCGGACTGGAAGGAAGATTACAGCCATGTGCGGCCCCACACCGC
CTTGGGCGGCATCGCCCCAGCGCAGGCCGCCGCACGAGCGCGAGCGCAATGTGGGCCGGGGCATGCCCCGGCCCACATTGCCATCACCGCCCATTTCGGG
CATCACGGGGAGACGGGGCTCTACCTCTGACTGGGATCAATCAGGGGAACAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 65 | 331 | + | No |
Description : First part of the transposase
ORF sequence :
MKRSRFSEEQIIGILKEQEAGQATADVCRRHGISSATFYKWKAKYGGLEVSEAKRLKALEDENARLKKLLAEAMLDNAVLKEISSKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 987 bp | 328 aa | 244 | 1230 | + | No |
Description : Second part of the transposase
ORF sequence :
GRKRSAEETAGRSHARQRRAEGNFLKKMVTPAARRIVVAHVRELFGLSERRACLIVGISRRVMRYRSCRPDDAPIRARLRELAAERRRFGFRRLGILLSR
EGVAMNRKKLLRLYREEKLAVRPRRGRKRALGCRAPMAIPQGPNQYWSLDFLSDAFTDGRRFRIFAVVDDFTRECLALVADTSLSGVRVARELDGLIAGR
GKPQMIVSDNGTEFTSMAMLRWSQDHRVEWHYIAPGKPMQNGFVESFNGKLRDECLNETLFGSINHARAVLADWKEDYSHVRPHTALGGIAPAQAAARAR
AQCGPGHAPAHIAITAHFGHHGETGLYL
EGVAMNRKKLLRLYREEKLAVRPRRGRKRALGCRAPMAIPQGPNQYWSLDFLSDAFTDGRRFRIFAVVDDFTRECLALVADTSLSGVRVARELDGLIAGR
GKPQMIVSDNGTEFTSMAMLRWSQDHRVEWHYIAPGKPMQNGFVESFNGKLRDECLNETLFGSINHARAVLADWKEDYSHVRPHTALGGIAPAQAAARAR
AQCGPGHAPAHIAITAHFGHHGETGLYL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1166 bp | 388 aa | 65 | 1230 | + | Yes |
Chemistry : DDE
ORF sequence :
MKRSRFSEEQIIGILKEQEAGQATADVCRRHGISSATFYKWKAKYGGLEVSEAKRLKALEDENARLKKLLAEAMLDNAVLKEISSKKMVTPAARRIVVAH
VRELFGLSERRACLIVGISRRVMRYRSCRPDDAPIRARLRELAAERRRFGFRRLGILLSREGVAMNRKKLLRLYREEKLAVRPRRGRKRALGCRAPMAIP
QGPNQYWSLDFLSDAFTDGRRFRIFAVVDDFTRECLALVADTSLSGVRVARELDGLIAGRGKPQMIVSDNGTEFTSMAMLRWSQDHRVEWHYIAPGKPMQ
NGFVESFNGKLRDECLNETLFGSINHARAVLADWKEDYSHVRPHTALGGIAPAQAAARARAQCGPGHAPAHIAITAHFGHHGETGLYL
VRELFGLSERRACLIVGISRRVMRYRSCRPDDAPIRARLRELAAERRRFGFRRLGILLSREGVAMNRKKLLRLYREEKLAVRPRRGRKRALGCRAPMAIP
QGPNQYWSLDFLSDAFTDGRRFRIFAVVDDFTRECLALVADTSLSGVRVARELDGLIAGRGKPQMIVSDNGTEFTSMAMLRWSQDHRVEWHYIAPGKPMQ
NGFVESFNGKLRDECLNETLFGSINHARAVLADWKEDYSHVRPHTALGGIAPAQAAARARAQCGPGHAPAHIAITAHFGHHGETGLYL
Blast result :
Comments
ISMamg1 is 94% (ORFA) aa similar to ISR1 and 71% (ORFB) aa similar to IS511. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Matsunaga,T., Okamura,Y., Fukuda,Y., Wahyudi,A.T., Murase,Y. and Takeyama,H. (2005) DNA Res. 12 (3), 157-166.