ISNmu2
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007614 | ND | Nitrosospira multiformis | Nitrosospira multiformis ATCC 25196 |
DNA section
IS Length : 1243 bp
Ends
IR Length : 33/47
IRL : TGATGTGCTCCCCATAAAACGTGCCATCTGGAATCTAGTAAAGTTCGTTT
IRR : TGATGTGCCCCCGGTAGTAGTGCCAGGGGCGTTCTAGCAAAGTTCAGTTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCTACCAGAA | GCCC | TACAATCGAT | 4 |
DNA sequence
TGATGTGCTCCCCATAAAACGTGCCATCTGGAATCTAGTAAAGTTCGTTTTTCGAGAGGAGAACGGACGTGAAGAAGCGATTTACGGATGAACAAATTAT
TGGATTTCTGAAGCAGGCAGATGGAGGCATTCCGGTCAGGGAATTGTGCCGCCAGCATGGTTTTTCCGATGCGTCGTTTTACACCTGGCGTGCCAGGTTT
GGTGGGATGACAGTATCGGATGCCAGGCGGTTGCGAGATCTTGAAGCAGAGAATGCTCAGCTCAAGAAGCTTTTAGCCGAGTCGCTTCTTGATGCTCAAG
CCTTGAAGGTTGCTTTGGGCCGAAAGCATTAACTCCACAGGCCAGGCGTGAGGCAGTAATGGCGATGCTTGAGCAGACGAACGTTTCCGAACGCCGTGCC
TGCCTGCTTGTGGATATATCACGTACGGTATTGCACTACATGTCCAAGGTGCGGCTGGAGAATGAGCAACTGCAAGGTCGAATGGTTGAGTTGGCCTCTG
AACGCCGTCGTTTTGGTTATCGACGCATTCATGCCCTGCTGCGGCGTGAGGGTGTTGAAGCCAATCACAAACGCATCTTTCGCCTTTACCAGGCTGCCGG
GTTGGCAGTAAAGCGCCGACGCAGGCGAGGGGGCGTAGCAGTTGAACGTGAGCAATTGGCATTGCCAAGCCAGCCCAACGAGGTATGGTCAATGGATTTC
ATCTGTGATGCACTGGCGAATGGCAGACGCATCAAGGTATTGACCATCGTTGATGACTTCACCAAGGAATCCATTGATCTGGTAGCTGAACATGGCATTT
CGGGACAACATGTCGTGCGCGTATTGGAGCAAGCTTCACAGTTTCGTGGGTTGCCCTTGGCCATTCGCACCGATCAGGGACCGGAGTTCACCAGCAAGGC
ACTGGATCAGTGGGCTTATAAGCATGGTGTCCAGCTCAAATTGATTGAGGCGGGCAAGCCGACGCAAAATGCATATATTGAGAGCTTCAACGGTCGGTTT
CGGGATGAGTGCCTGAACGATCACTGGTTTACCAGCCTGGCTGAGGCACGGATTCGGATATCAGCCTGGCGGCGCGACTACAACGAGCATCGACCGCACA
GTGCACTAAATTACCTAACCCCGGCAGAATTTGCAGCCAACTGCCGCAGGCAAGAAGAAATAAACGGCTGTGAAAGAAATGGAAGCTAATTTATAACTGA
ACTTTGCTAGAACGCCCCTGGCACTACTACCGGGGGCACATCA
TGGATTTCTGAAGCAGGCAGATGGAGGCATTCCGGTCAGGGAATTGTGCCGCCAGCATGGTTTTTCCGATGCGTCGTTTTACACCTGGCGTGCCAGGTTT
GGTGGGATGACAGTATCGGATGCCAGGCGGTTGCGAGATCTTGAAGCAGAGAATGCTCAGCTCAAGAAGCTTTTAGCCGAGTCGCTTCTTGATGCTCAAG
CCTTGAAGGTTGCTTTGGGCCGAAAGCATTAACTCCACAGGCCAGGCGTGAGGCAGTAATGGCGATGCTTGAGCAGACGAACGTTTCCGAACGCCGTGCC
TGCCTGCTTGTGGATATATCACGTACGGTATTGCACTACATGTCCAAGGTGCGGCTGGAGAATGAGCAACTGCAAGGTCGAATGGTTGAGTTGGCCTCTG
AACGCCGTCGTTTTGGTTATCGACGCATTCATGCCCTGCTGCGGCGTGAGGGTGTTGAAGCCAATCACAAACGCATCTTTCGCCTTTACCAGGCTGCCGG
GTTGGCAGTAAAGCGCCGACGCAGGCGAGGGGGCGTAGCAGTTGAACGTGAGCAATTGGCATTGCCAAGCCAGCCCAACGAGGTATGGTCAATGGATTTC
ATCTGTGATGCACTGGCGAATGGCAGACGCATCAAGGTATTGACCATCGTTGATGACTTCACCAAGGAATCCATTGATCTGGTAGCTGAACATGGCATTT
CGGGACAACATGTCGTGCGCGTATTGGAGCAAGCTTCACAGTTTCGTGGGTTGCCCTTGGCCATTCGCACCGATCAGGGACCGGAGTTCACCAGCAAGGC
ACTGGATCAGTGGGCTTATAAGCATGGTGTCCAGCTCAAATTGATTGAGGCGGGCAAGCCGACGCAAAATGCATATATTGAGAGCTTCAACGGTCGGTTT
CGGGATGAGTGCCTGAACGATCACTGGTTTACCAGCCTGGCTGAGGCACGGATTCGGATATCAGCCTGGCGGCGCGACTACAACGAGCATCGACCGCACA
GTGCACTAAATTACCTAACCCCGGCAGAATTTGCAGCCAACTGCCGCAGGCAAGAAGAAATAAACGGCTGTGAAAGAAATGGAAGCTAATTTATAACTGA
ACTTTGCTAGAACGCCCCTGGCACTACTACCGGGGGCACATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 263 bp | 87 aa | 70 | 332 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRFTDEQIIGFLKQADGGIPVRELCRQHGFSDASFYTWRARFGGMTVSDARRLRDLEAENAQLKKLLAESLLDAQALKVALGRKH
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 897 bp | 298 aa | 293 | 1189 | + | No |
Description : Second part of the transposase
ORF sequence :
CSSLEGCFGPKALTPQARREAVMAMLEQTNVSERRACLLVDISRTVLHYMSKVRLENEQLQGRMVELASERRRFGYRRIHALLRREGVEANHKRIFRLYQ
AAGLAVKRRRRRGGVAVEREQLALPSQPNEVWSMDFICDALANGRRIKVLTIVDDFTKESIDLVAEHGISGQHVVRVLEQASQFRGLPLAIRTDQGPEFT
SKALDQWAYKHGVQLKLIEAGKPTQNAYIESFNGRFRDECLNDHWFTSLAEARIRISAWRRDYNEHRPHSALNYLTPAEFAANCRRQEEINGCERNGS
AAGLAVKRRRRRGGVAVEREQLALPSQPNEVWSMDFICDALANGRRIKVLTIVDDFTKESIDLVAEHGISGQHVVRVLEQASQFRGLPLAIRTDQGPEFT
SKALDQWAYKHGVQLKLIEAGKPTQNAYIESFNGRFRDECLNDHWFTSLAEARIRISAWRRDYNEHRPHSALNYLTPAEFAANCRRQEEINGCERNGS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1120 bp | 373 aa | 70 | 1189 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRFTDEQIIGFLKQADGGIPVRELCRQHGFSDASFYTWRARFGGMTVSDARRLRDLEAENAQLKKLLAESLLDAQALKVALGRKALTPQARREAVMAM
LEQTNVSERRACLLVDISRTVLHYMSKVRLENEQLQGRMVELASERRRFGYRRIHALLRREGVEANHKRIFRLYQAAGLAVKRRRRRGGVAVEREQLALP
SQPNEVWSMDFICDALANGRRIKVLTIVDDFTKESIDLVAEHGISGQHVVRVLEQASQFRGLPLAIRTDQGPEFTSKALDQWAYKHGVQLKLIEAGKPTQ
NAYIESFNGRFRDECLNDHWFTSLAEARIRISAWRRDYNEHRPHSALNYLTPAEFAANCRRQEEINGCERNGS
LEQTNVSERRACLLVDISRTVLHYMSKVRLENEQLQGRMVELASERRRFGYRRIHALLRREGVEANHKRIFRLYQAAGLAVKRRRRRGGVAVEREQLALP
SQPNEVWSMDFICDALANGRRIKVLTIVDDFTKESIDLVAEHGISGQHVVRVLEQASQFRGLPLAIRTDQGPEFTSKALDQWAYKHGVQLKLIEAGKPTQ
NAYIESFNGRFRDECLNDHWFTSLAEARIRISAWRRDYNEHRPHSALNYLTPAEFAANCRRQEEINGCERNGS
Blast result :
Comments
ISNmu2 is 91% (ORFA) and 85% (ORFB) aa similar to IS407. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A. and Richardson,P. (2006) Direct submission GenBank.