ISSpy1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007297 | ND | Streptococcus pyogenes | Streptococcus pyogenes MGAS5005 |
DNA section
IS Length : 1242 bp
Ends
IR Length : 27/36
IRL : TGGACTTGGTATAATAAAGTAGACACAAAATTAAGTGGAAACGAGGAAAA
IRR : TGGACTTGTCAAGAAAAAATAGACATAAAGTTAAGCTAATGAGTAACTAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAGGTTGGA | GGGTATGAAG | 0 |
DNA sequence
TGGACTTGGTATAATAAAGTAGACACAAAATTAAGTGGAAACGAGGAAAAGTCATTATGCCAAGAAAAACCTTTGATAAAGCCTTCAAACTCTCTGCTGT
AAAACTCATCCTTGAGGAAGAGTAGCCTGTAAAAATGGCTAGTTCAACTTTAAAAATTCATCCCAATAGTCTTTATCGATGGATTCAAGAATATGAAAAA
TATGGAGAAAGTGCGTTCCCAGGACATGGGAGCGCACTTCGTCATGCTCAATTTGAGACAAAAAAACTTGAAAAAGAGCACAAACTGCTACAGGAGGAAT
TAGCTCTTCTAAAAAAGTTCCAGGTCTTTTTGAAGCCAAACCGGAAATAAAATTTCAGTTTCTGAAGGAAAATAGTGTTAGCTTGAATAGTCATCACGCT
TGTCAAACTCTTCAAGTCTCCCGTTCAGGTTTCTATGCTTATTTAAAACGTCGTCCCTCTTCAAGGCATGTTGAGAATGAAGCTTTGAAGGAAATGATTA
AAGCCATTTTCTATGAACATAAAGAGCGTTACGGAAGTGTACGAATTACCCAAGAACTTTGTAGACGTGGCATCCATGTCAATTATAAACGTGTTGGTAG
ACTCCTTCATCAGCTAGGTCTCTATGCTAAAGGAAGTCGATATCAATATAAATACTATAACCGTCGACGTTCTTCATTAACTCGTCCCAATCTTGTTAAT
CAATGTTTCCAAGCGACTGGTAAGAATAAATTATGGCTAGGAGACCTAACCTACATTCCTACACAAGAAGGAATACTTTATGTGTCTGTCTTTATAGACG
TTTATAGTAGAAAGATTGTTGGTTGGGCAATGGGCCGACGTATGCAAGACAAGTTGGTAACAGAAGCTTTCAATCAAGCTTATAATAGAGAAAAGCCTAA
AGAAGGGGTAATCGTCCATACAGATCAGGGTTCTCAATATACCGGAGCATGATTTCAGGACCTACTTAGACAGAAAAAATGTAAGTCCAGGATGAGTCGA
AAAGGTAATCCATACGATAATGCACTCATGGAATCCTTTTATAAAACATTAAAAAGAGAGCTTGTCAATGATGCTCATTTCGCAACTATTGAGCAAGCTC
AGCTTGAGATTTTTAAATATAGTGAGACTTACTATAACCCTAAACGTCTGCATTCAGCTTTAGGGTATCTCTCACCTGTTGAATTTGAAAAAATAGTTAC
TCATTAGCTTAACTTTATGTCTATTTTTTCTTGACAAGTCCA
AAAACTCATCCTTGAGGAAGAGTAGCCTGTAAAAATGGCTAGTTCAACTTTAAAAATTCATCCCAATAGTCTTTATCGATGGATTCAAGAATATGAAAAA
TATGGAGAAAGTGCGTTCCCAGGACATGGGAGCGCACTTCGTCATGCTCAATTTGAGACAAAAAAACTTGAAAAAGAGCACAAACTGCTACAGGAGGAAT
TAGCTCTTCTAAAAAAGTTCCAGGTCTTTTTGAAGCCAAACCGGAAATAAAATTTCAGTTTCTGAAGGAAAATAGTGTTAGCTTGAATAGTCATCACGCT
TGTCAAACTCTTCAAGTCTCCCGTTCAGGTTTCTATGCTTATTTAAAACGTCGTCCCTCTTCAAGGCATGTTGAGAATGAAGCTTTGAAGGAAATGATTA
AAGCCATTTTCTATGAACATAAAGAGCGTTACGGAAGTGTACGAATTACCCAAGAACTTTGTAGACGTGGCATCCATGTCAATTATAAACGTGTTGGTAG
ACTCCTTCATCAGCTAGGTCTCTATGCTAAAGGAAGTCGATATCAATATAAATACTATAACCGTCGACGTTCTTCATTAACTCGTCCCAATCTTGTTAAT
CAATGTTTCCAAGCGACTGGTAAGAATAAATTATGGCTAGGAGACCTAACCTACATTCCTACACAAGAAGGAATACTTTATGTGTCTGTCTTTATAGACG
TTTATAGTAGAAAGATTGTTGGTTGGGCAATGGGCCGACGTATGCAAGACAAGTTGGTAACAGAAGCTTTCAATCAAGCTTATAATAGAGAAAAGCCTAA
AGAAGGGGTAATCGTCCATACAGATCAGGGTTCTCAATATACCGGAGCATGATTTCAGGACCTACTTAGACAGAAAAAATGTAAGTCCAGGATGAGTCGA
AAAGGTAATCCATACGATAATGCACTCATGGAATCCTTTTATAAAACATTAAAAAGAGAGCTTGTCAATGATGCTCATTTCGCAACTATTGAGCAAGCTC
AGCTTGAGATTTTTAAATATAGTGAGACTTACTATAACCCTAAACGTCTGCATTCAGCTTTAGGGTATCTCTCACCTGTTGAATTTGAAAAAATAGTTAC
TCATTAGCTTAACTTTATGTCTATTTTTTCTTGACAAGTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 57 | 350 | + | No |
Description : First part of the transposase
ORF sequence :
MPRKTFDKAFKLSAVKLILEEE*PVKMASSTLKIHPNSLYRWIQEYEKYGESAFPGHGSALRHAQFETKKLEKEHKLLQEELALLKKFQVFLKPNRK
Blast result :Comments : There is a STOP codon in frame. The transposase will be probably inactive.ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 272 | 1195 | + | No |
Description : Second part of the transposase
ORF sequence :
KRAQTATGGISSSKKVPGLFEAKPEIKFQFLKENSVSLNSHHACQTLQVSRSGFYAYLKRRPSSRHVENEALKEMIKAIFYEHKERYGSVRITQELCRRG
IHVNYKRVGRLLHQLGLYAKGSRYQYKYYNRRRSSLTRPNLVNQCFQATGKNKLWLGDLTYIPTQEGILYVSVFIDVYSRKIVGWAMGRRMQDKLVTEAF
NQAYNREKPKEGVIVHTDQGSQYTGA*FQDLLRQKKCKSRMSRKGNPYDNALMESFYKTLKRELVNDAHFATIEQAQLEIFKYSETYYNPKRLHSALGYL
SPVEFEK
IHVNYKRVGRLLHQLGLYAKGSRYQYKYYNRRRSSLTRPNLVNQCFQATGKNKLWLGDLTYIPTQEGILYVSVFIDVYSRKIVGWAMGRRMQDKLVTEAF
NQAYNREKPKEGVIVHTDQGSQYTGA*FQDLLRQKKCKSRMSRKGNPYDNALMESFYKTLKRELVNDAHFATIEQAQLEIFKYSETYYNPKRLHSALGYL
SPVEFEK
Blast result :Comments : There is a STOP codon in frame. The transposase will be probably inactive.ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1139 bp | 379 aa | 57 | 1195 | + | Yes |
Chemistry : DDE
ORF sequence :
MPRKTFDKAFKLSAVKLILEEE*PVKMASSTLKIHPNSLYRWIQEYEKYGESAFPGHGSALRHAQFETKKLEKEHKLLQEELALLKKVPGLFEAKPEIKF
QFLKENSVSLNSHHACQTLQVSRSGFYAYLKRRPSSRHVENEALKEMIKAIFYEHKERYGSVRITQELCRRGIHVNYKRVGRLLHQLGLYAKGSRYQYKY
YNRRRSSLTRPNLVNQCFQATGKNKLWLGDLTYIPTQEGILYVSVFIDVYSRKIVGWAMGRRMQDKLVTEAFNQAYNREKPKEGVIVHTDQGSQYTGA*F
QDLLRQKKCKSRMSRKGNPYDNALMESFYKTLKRELVNDAHFATIEQAQLEIFKYSETYYNPKRLHSALGYLSPVEFEK
QFLKENSVSLNSHHACQTLQVSRSGFYAYLKRRPSSRHVENEALKEMIKAIFYEHKERYGSVRITQELCRRGIHVNYKRVGRLLHQLGLYAKGSRYQYKY
YNRRRSSLTRPNLVNQCFQATGKNKLWLGDLTYIPTQEGILYVSVFIDVYSRKIVGWAMGRRMQDKLVTEAFNQAYNREKPKEGVIVHTDQGSQYTGA*F
QDLLRQKKCKSRMSRKGNPYDNALMESFYKTLKRELVNDAHFATIEQAQLEIFKYSETYYNPKRLHSALGYLSPVEFEK
Blast result :
Comments
ISSpy1 is 82% (ORFA) and 78% (ORFB) aa similar to IS1068. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
There are STOP codon in frame. The transposase will probably be inactive.
Update : June 30 2017.
There are STOP codon in frame. The transposase will probably be inactive.
Update : June 30 2017.
References
1] Sumby,P., Porcella,S.F., Madrigal,A.G., Barbian,K.D., Virtaneva,K., Ricklefs,S.M., Sturdevant,D.E., Graham,M.R., Vuopio-Varkila,J., Hoe,N.P. and Musser,J.M. (2005) J. Infect. Dis. 192 (5), 771-782