ISDha7
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007907 | ND | Desulfitobacterium hafniense | Desulfitobacterium hafniense Y51 |
DNA section
IS Length : 1499 bp
Ends
IR Length : 26/39
IRL : TGACCCATACCCGGTTTGTCGGACACAGGAGAGTTAGGGTAAAATAAGAC
IRR : TGACCTTTTCTCGATAAAACGGACATTCTAGAGTTAGGGATTCTCCCAGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAACGAAGAT | CCA | TCCTGGAGCG | 3 |
DNA sequence
TGACCCATACCCGGTTTGTCGGACACAGGAGAGTTAGGGTAAAATAAGACCTGTGGAGGATGATAAACGTGTCAAAAAACGGGAAACGGTATAATCAAGA
ATTCAAACATGACATTATACGCTTAATCACTGAAGAAAACCGTTCAGTTTCAAGTGTGGTTCAAGATTTTGGGGTCAATGAGCAAACTGTGCGCAACTGG
CTTAAGCAAACCAAAGAGAGGCAAGATCCTATCAAGACCAAGATAGCCGAGCTTGAAGCTGAGCTTAAAGCAAGGGATAAAAAGATTGCCGATCAGGAGT
TAACGATAGATATCTTAAAAAAGGCTACCGCCATCTTCGCCCAAAGCAACCGGAAATAATCTATCAGATGATTAAAAAAGCCAGCAGCTCCAGCTGCCCG
GTTGAGAAGATGTGCGAAACCTTAGAGGTATCTCGGAGCGGTTATTATGATTGGGATAGGCGTGAACCCAGCAAGCGTCAAAAAGAGAACGAAACGATTC
TTAAGGTGATGAAGGAAAGCCATACCAAGGCTCAGGCAATGATTGGTCTGGATAAACTTTGGAGCGATGTCAAAGAGGCAGGGTTTCAATGCGGTAGAAA
CCGGGTTTACCGATTACAAAAGCAACATGGACTATACAGTGTTCGAAAGAAGCCCTATCGCGTCTGTCTCACCGACTCGAATCATGATTTGCCCAAAGCC
CCGAATCTCTTAAACCAAAAATTTAAGGTTGAGCACCCGAATAAAGTCTGGGTCACGGATATCACAGAATTTAAAACAGCTAAAGGAAGCAAGCTGTATC
TGGCTGCCATCAAGGACTTATTTCATAAAGAAATTGTGGGTTGGGCTTTGGCTGAACACATGAGGACTGAGCTCTGTTTGGAAGCCTTAAGGAACGCTGT
CAAGCGTCATAGACCGCTCAAAGGGTTGATTCATCATTCGGATCAAGGGCGGCAATACTGCAGCACCGTCTATGTCGAAGAATTAAAGCACTGGGGAATG
ATACGAAGTATGAGCAGAAAGGGCAACCCCTTTGATAATGCCTGCGCAGAGTCTTTTTTCAGCACCATTAAATCAGAAAGGCTCCATCACAAAACATACA
AAGACATCGAGGAAGCAAGGCGGGATATCTTTTGGTACATCGAATGTTTTTATAATCGACAACGGAGGCATCAAGCGTTAGGGAATCTGACACCGGCAGC
TTTTTTAAAGAAGCACTGTGAGACCCAAAAGGCTGCTTAGGGTCAACTTGGTAGGCGCCTGTCAAGGGGCGGTGTCAGCCGAGACGAAGTCCTTGCCCTT
GATAGGCCAACTAGCTAAGACCCTTATGTCTCAGAACAGTTTAAAGACTGTTCTGCTGCCACCCGGCGAGGGCGGGGTTTCAGGGGCTGGCCCCTGAGAG
AGCAGCTTGGAGCATTTACTTAAGATGAAAAAAGAAGCAATCCTGCGATTCTGGGAGAATCCCTAACTCTAGAATGTCCGTTTTATCGAGAAAAGGTCA
ATTCAAACATGACATTATACGCTTAATCACTGAAGAAAACCGTTCAGTTTCAAGTGTGGTTCAAGATTTTGGGGTCAATGAGCAAACTGTGCGCAACTGG
CTTAAGCAAACCAAAGAGAGGCAAGATCCTATCAAGACCAAGATAGCCGAGCTTGAAGCTGAGCTTAAAGCAAGGGATAAAAAGATTGCCGATCAGGAGT
TAACGATAGATATCTTAAAAAAGGCTACCGCCATCTTCGCCCAAAGCAACCGGAAATAATCTATCAGATGATTAAAAAAGCCAGCAGCTCCAGCTGCCCG
GTTGAGAAGATGTGCGAAACCTTAGAGGTATCTCGGAGCGGTTATTATGATTGGGATAGGCGTGAACCCAGCAAGCGTCAAAAAGAGAACGAAACGATTC
TTAAGGTGATGAAGGAAAGCCATACCAAGGCTCAGGCAATGATTGGTCTGGATAAACTTTGGAGCGATGTCAAAGAGGCAGGGTTTCAATGCGGTAGAAA
CCGGGTTTACCGATTACAAAAGCAACATGGACTATACAGTGTTCGAAAGAAGCCCTATCGCGTCTGTCTCACCGACTCGAATCATGATTTGCCCAAAGCC
CCGAATCTCTTAAACCAAAAATTTAAGGTTGAGCACCCGAATAAAGTCTGGGTCACGGATATCACAGAATTTAAAACAGCTAAAGGAAGCAAGCTGTATC
TGGCTGCCATCAAGGACTTATTTCATAAAGAAATTGTGGGTTGGGCTTTGGCTGAACACATGAGGACTGAGCTCTGTTTGGAAGCCTTAAGGAACGCTGT
CAAGCGTCATAGACCGCTCAAAGGGTTGATTCATCATTCGGATCAAGGGCGGCAATACTGCAGCACCGTCTATGTCGAAGAATTAAAGCACTGGGGAATG
ATACGAAGTATGAGCAGAAAGGGCAACCCCTTTGATAATGCCTGCGCAGAGTCTTTTTTCAGCACCATTAAATCAGAAAGGCTCCATCACAAAACATACA
AAGACATCGAGGAAGCAAGGCGGGATATCTTTTGGTACATCGAATGTTTTTATAATCGACAACGGAGGCATCAAGCGTTAGGGAATCTGACACCGGCAGC
TTTTTTAAAGAAGCACTGTGAGACCCAAAAGGCTGCTTAGGGTCAACTTGGTAGGCGCCTGTCAAGGGGCGGTGTCAGCCGAGACGAAGTCCTTGCCCTT
GATAGGCCAACTAGCTAAGACCCTTATGTCTCAGAACAGTTTAAAGACTGTTCTGCTGCCACCCGGCGAGGGCGGGGTTTCAGGGGCTGGCCCCTGAGAG
AGCAGCTTGGAGCATTTACTTAAGATGAAAAAAGAAGCAATCCTGCGATTCTGGGAGAATCCCTAACTCTAGAATGTCCGTTTTATCGAGAAAAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 60 | 359 | + | No |
Description : First part of the transposase
ORF sequence :
MINVSKNGKRYNQEFKHDIIRLITEENRSVSSVVQDFGVNEQTVRNWLKQTKERQDPIKTKIAELEAELKARDKKIADQELTIDILKKATAIFAQSNRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 960 bp | 319 aa | 281 | 1240 | + | No |
Description : Second part of the transposase
ORF sequence :
KDCRSGVNDRYLKKGYRHLRPKQPEIIYQMIKKASSSSCPVEKMCETLEVSRSGYYDWDRREPSKRQKENETILKVMKESHTKAQAMIGLDKLWSDVKEA
GFQCGRNRVYRLQKQHGLYSVRKKPYRVCLTDSNHDLPKAPNLLNQKFKVEHPNKVWVTDITEFKTAKGSKLYLAAIKDLFHKEIVGWALAEHMRTELCL
EALRNAVKRHRPLKGLIHHSDQGRQYCSTVYVEELKHWGMIRSMSRKGNPFDNACAESFFSTIKSERLHHKTYKDIEEARRDIFWYIECFYNRQRRHQAL
GNLTPAAFLKKHCETQKAA
GFQCGRNRVYRLQKQHGLYSVRKKPYRVCLTDSNHDLPKAPNLLNQKFKVEHPNKVWVTDITEFKTAKGSKLYLAAIKDLFHKEIVGWALAEHMRTELCL
EALRNAVKRHRPLKGLIHHSDQGRQYCSTVYVEELKHWGMIRSMSRKGNPFDNACAESFFSTIKSERLHHKTYKDIEEARRDIFWYIECFYNRQRRHQAL
GNLTPAAFLKKHCETQKAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1181 bp | 393 aa | 60 | 1240 | + | Yes |
Chemistry : DDE
ORF sequence :
MINVSKNGKRYNQEFKHDIIRLITEENRSVSSVVQDFGVNEQTVRNWLKQTKERQDPIKTKIAELEAELKARDKKIADQELTIDILKKGYRHLRPKQPEI
IYQMIKKASSSSCPVEKMCETLEVSRSGYYDWDRREPSKRQKENETILKVMKESHTKAQAMIGLDKLWSDVKEAGFQCGRNRVYRLQKQHGLYSVRKKPY
RVCLTDSNHDLPKAPNLLNQKFKVEHPNKVWVTDITEFKTAKGSKLYLAAIKDLFHKEIVGWALAEHMRTELCLEALRNAVKRHRPLKGLIHHSDQGRQY
CSTVYVEELKHWGMIRSMSRKGNPFDNACAESFFSTIKSERLHHKTYKDIEEARRDIFWYIECFYNRQRRHQALGNLTPAAFLKKHCETQKAA
IYQMIKKASSSSCPVEKMCETLEVSRSGYYDWDRREPSKRQKENETILKVMKESHTKAQAMIGLDKLWSDVKEAGFQCGRNRVYRLQKQHGLYSVRKKPY
RVCLTDSNHDLPKAPNLLNQKFKVEHPNKVWVTDITEFKTAKGSKLYLAAIKDLFHKEIVGWALAEHMRTELCLEALRNAVKRHRPLKGLIHHSDQGRQY
CSTVYVEELKHWGMIRSMSRKGNPFDNACAESFFSTIKSERLHHKTYKDIEEARRDIFWYIECFYNRQRRHQALGNLTPAAFLKKHCETQKAA
Blast result :
Comments
ISDha7 is 61 % (ORFA) aa similar to ISCce4 and 63% (ORFB) aa similar to ISCce3. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Nonaka,H., Keresztes,G., Shinoda,Y., Ikenaga,Y., Abe,M., Naito,K., Inatomi,K., Furukawa,K., Inui,M. and Yukawa,H. (2006) J. Bacteriol. 188 (6), 2262-2274.