ISOgr1
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAOT01000036.1 | ND | Oceanicola granulosus | Oceanicola granulosus HTCC2516 |
DNA section
IS Length : 1255 bp
Ends
IR Length : 33/45
IRL : TGAATAGCCCCGTGTCTTGTGGACGCCTTCTTCGCTAATTTTGAGGCAAG
IRR : TGAATAGCCCCGCGTTTCTTAGACGCCCTCGTGCCTCAGTTTCTGCTGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCGCCGAAG | GACACCGACT | 0 |
DNA sequence
TGAATAGCCCCGTGTCTTGTGGACGCCTTCTTCGCTAATTTTGAGGCAAGGAGGCCATGATGAGCAGCAGCAAGTTCAGCGACGAGTTCAAGCGGGATGC
CGTCGCGCAGATCACCGAGCGGGGCTATCCGGTCAGGGAGGTGTCGAAGCGCCTCGGGGTCAGCCCGTATTCGCTCTACGCCTGGAATAAGAAGTTCGCG
CAGGCGTCGTCCGGTGAGGCGGAGAAGGACACCGAGATCCGGCGCCTGAAGAAGGAGCTGGCGCGGGTCTCGGAGGAGCGTGACATCCTAAAAAAGGGTC
GCCATTGGTGCGCCATTGGTCCGAGCGACAATGGCGACGCGTATTTCGCCAGGGATGCAAAGTGAGATACGCGTTCGTGGCCGAGCATCGCGGGCAGTTC
TCTGTCCGGGCGATGTGCCGGTGTCTGCGCATCCAACCGAGCGGCTTCTATGCGTGGTTGAAGGCGCCGGTGAGCAAGCGTGCCCAGGAAGACGCCCGCC
AGACCGAACTGCTGAAGGAAGCCTGGGTCGAAAGCGGCAAGGTCTATGGGCACCGCAAGCTTCACGACGATCTTCTCGAACAGGGCGAAGACGTCTGCCT
GAACCGCGTCGCGCGCCTGACCAGGCTGGCCGGGATCACGGCACAGATCGGCTACAAGCGCCGCCCAGGCAAGTATGGTGGCAAACCCTCGGTCGTGGTC
GACAACACGCTCGATCGGCAGTTCGACGTGGAGGCGCCGGACAGGGTCTGGGTGACCGACATCACCTACATCCGCACCCAGGAAGGCTTCGCCTACCTCG
CTGTGGTCATCGACCTGTTCTCTCGGCGCGTGGTCGGATGGTCCATGCAGTCGCGCCAGACGACCGACGTCGTGCTCCAGGCTCTGCTCATGGCCACATG
GCGGAGGAAGCCGAAGGCCAAGGTGCTGATCCACTCCGATCAGGGAAGTCAGTTCACCAGCATGGACTGGGCCGCGTTCCTGAAAGCCCACGACCTCGAG
CATTCCATGAGCCGTCGAGGCAACTGCCACGATAACGCCGTCGCCGAGAGCTTCTTCAACCTGCTGAAGCGCGAGAGGATCAGGCGTCGCACCTACCGCA
CCCGAAAGGAAGCCAGGCGAGACGTGTTCGACTACATCGAGATGTTCTACAACCCGAAGCGCAAGCACGCGCGAAACGGAATGCTGTCGCCACTCGAGTT
CGTGCGGCAGCAGAAACTGAGGCACGAGGGCGTCTAAGAAACGCGGGGCTATTCA
CGTCGCGCAGATCACCGAGCGGGGCTATCCGGTCAGGGAGGTGTCGAAGCGCCTCGGGGTCAGCCCGTATTCGCTCTACGCCTGGAATAAGAAGTTCGCG
CAGGCGTCGTCCGGTGAGGCGGAGAAGGACACCGAGATCCGGCGCCTGAAGAAGGAGCTGGCGCGGGTCTCGGAGGAGCGTGACATCCTAAAAAAGGGTC
GCCATTGGTGCGCCATTGGTCCGAGCGACAATGGCGACGCGTATTTCGCCAGGGATGCAAAGTGAGATACGCGTTCGTGGCCGAGCATCGCGGGCAGTTC
TCTGTCCGGGCGATGTGCCGGTGTCTGCGCATCCAACCGAGCGGCTTCTATGCGTGGTTGAAGGCGCCGGTGAGCAAGCGTGCCCAGGAAGACGCCCGCC
AGACCGAACTGCTGAAGGAAGCCTGGGTCGAAAGCGGCAAGGTCTATGGGCACCGCAAGCTTCACGACGATCTTCTCGAACAGGGCGAAGACGTCTGCCT
GAACCGCGTCGCGCGCCTGACCAGGCTGGCCGGGATCACGGCACAGATCGGCTACAAGCGCCGCCCAGGCAAGTATGGTGGCAAACCCTCGGTCGTGGTC
GACAACACGCTCGATCGGCAGTTCGACGTGGAGGCGCCGGACAGGGTCTGGGTGACCGACATCACCTACATCCGCACCCAGGAAGGCTTCGCCTACCTCG
CTGTGGTCATCGACCTGTTCTCTCGGCGCGTGGTCGGATGGTCCATGCAGTCGCGCCAGACGACCGACGTCGTGCTCCAGGCTCTGCTCATGGCCACATG
GCGGAGGAAGCCGAAGGCCAAGGTGCTGATCCACTCCGATCAGGGAAGTCAGTTCACCAGCATGGACTGGGCCGCGTTCCTGAAAGCCCACGACCTCGAG
CATTCCATGAGCCGTCGAGGCAACTGCCACGATAACGCCGTCGCCGAGAGCTTCTTCAACCTGCTGAAGCGCGAGAGGATCAGGCGTCGCACCTACCGCA
CCCGAAAGGAAGCCAGGCGAGACGTGTTCGACTACATCGAGATGTTCTACAACCCGAAGCGCAAGCACGCGCGAAACGGAATGCTGTCGCCACTCGAGTT
CGTGCGGCAGCAGAAACTGAGGCACGAGGGCGTCTAAGAAACGCGGGGCTATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 58 | 366 | + | No |
Description : First part of the transposase
ORF sequence :
MMSSSKFSDEFKRDAVAQITERGYPVREVSKRLGVSPYSLYAWNKKFAQASSGEAEKDTEIRRLKKELARVSEERDILKKGRHWCAIGPSDNGDAYFARD
AK
AK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 954 bp | 317 aa | 284 | 1237 | + | No |
Description : Second part of the transposase
ORF sequence :
HPKKGSPLVRHWSERQWRRVFRQGCKVRYAFVAEHRGQFSVRAMCRCLRIQPSGFYAWLKAPVSKRAQEDARQTELLKEAWVESGKVYGHRKLHDDLLEQ
GEDVCLNRVARLTRLAGITAQIGYKRRPGKYGGKPSVVVDNTLDRQFDVEAPDRVWVTDITYIRTQEGFAYLAVVIDLFSRRVVGWSMQSRQTTDVVLQA
LLMATWRRKPKAKVLIHSDQGSQFTSMDWAAFLKAHDLEHSMSRRGNCHDNAVAESFFNLLKRERIRRRTYRTRKEARRDVFDYIEMFYNPKRKHARNGM
LSPLEFVRQQKLRHEGV
GEDVCLNRVARLTRLAGITAQIGYKRRPGKYGGKPSVVVDNTLDRQFDVEAPDRVWVTDITYIRTQEGFAYLAVVIDLFSRRVVGWSMQSRQTTDVVLQA
LLMATWRRKPKAKVLIHSDQGSQFTSMDWAAFLKAHDLEHSMSRRGNCHDNAVAESFFNLLKRERIRRRTYRTRKEARRDVFDYIEMFYNPKRKHARNGM
LSPLEFVRQQKLRHEGV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1180 bp | 393 aa | 58 | 1237 | + | Yes |
Chemistry : DDE
ORF sequence :
MMSSSKFSDEFKRDAVAQITERGYPVREVSKRLGVSPYSLYAWNKKFAQASSGEAEKDTEIRRLKKELARVSEERDILKKGSPLVRHWSERQWRRVFRQG
CKVRYAFVAEHRGQFSVRAMCRCLRIQPSGFYAWLKAPVSKRAQEDARQTELLKEAWVESGKVYGHRKLHDDLLEQGEDVCLNRVARLTRLAGITAQIGY
KRRPGKYGGKPSVVVDNTLDRQFDVEAPDRVWVTDITYIRTQEGFAYLAVVIDLFSRRVVGWSMQSRQTTDVVLQALLMATWRRKPKAKVLIHSDQGSQF
TSMDWAAFLKAHDLEHSMSRRGNCHDNAVAESFFNLLKRERIRRRTYRTRKEARRDVFDYIEMFYNPKRKHARNGMLSPLEFVRQQKLRHEGV
CKVRYAFVAEHRGQFSVRAMCRCLRIQPSGFYAWLKAPVSKRAQEDARQTELLKEAWVESGKVYGHRKLHDDLLEQGEDVCLNRVARLTRLAGITAQIGY
KRRPGKYGGKPSVVVDNTLDRQFDVEAPDRVWVTDITYIRTQEGFAYLAVVIDLFSRRVVGWSMQSRQTTDVVLQALLMATWRRKPKAKVLIHSDQGSQF
TSMDWAAFLKAHDLEHSMSRRGNCHDNAVAESFFNLLKRERIRRRTYRTRKEARRDVFDYIEMFYNPKRKHARNGMLSPLEFVRQQKLRHEGV
Blast result :
Comments
Genome in progress.
ISOgr1 is 76 % (ORFA) aa similar to I ISGpr2 and 79% (ORFB) aa similar to ISPsy29. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISOgr1 is 76 % (ORFA) aa similar to I ISGpr2 and 79% (ORFB) aa similar to ISPsy29. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Giovannoni,S.J., Cho,J.-C., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.