ISYps7
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAKT02000001 | ND | Yersinia pseudotuberculosis | Yersinia pestis CO92 Yersinia pseudotuberculosis IP 31758 Yersinia pestis Angola Yersinia pestis Antiqua Yersinia pestis FV-1 Yersinia pestis KIM Yersinia pestis Nepal516 Yersinia pestis biovar Microtus 91001 Yersinia pestis biovar Orientalis IP275 |
DNA section
IS Length : 737 bp
Ends
IR Length : 16/19
IRL : GGTCATGCATTCAATGCGGAGATCATCAGGTAAAATCCTCGCATTTATCT
IRR : GGTCATGTATTCAATCTGGTTATTAGCATCACACTAGGTAATGCTCACGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNNNNNN | GAATTTTT | ATTTTTATGGTG | 8 |
DNA sequence
GGTCATGCATTCAATGCGGAGATCATCAGGTAAAATCCTCGCATTTATCTGAGAGGGTTTGATGGCTAAGGTTGATGTGAAATGTCCATTTTGCGAGCAG
TTCCACCCTGTTAAAAAGCACGGGCCAGGACGCACAGGGCATCAGCGCTACCGCTGTCAGGCCTGCCGACGGAGCTTTCAACTAGAGTACGAGTATCGTG
CCTGCCACCCCGGTATGAAAGAGCAAATTGTCGATCTTGCTATGAACAATGCCGGTATTCGTGATACTGCACGGGCGTTGCATATCAGTATCAATGCGGT
CATGCGCACTTTAAAAAACTCTCGCCGCAGCGTGTAACCACGCTCCCACTGGATAACCTGCAAATTCAGCTTATCTGTGAAGTCGATGAAATGTGGTCAT
TTGTGGGCAACAAAAAGCAGCAACGTTGGCTTTGGTATGCCTGGGAGCCTCGCCTCAAACGTATTATTGCTCATGTTTTTGGTCGTCGGAGCAAAAAGAC
ATTTCGCCAATTATTGGGGTTGCTGTCAGGTTTCAATATCGTCTTCTGGTGTACCGACAACTTCAGTGCTTATGAGATGTTGCCGGATGAAAAACACATC
AGGAGCAAGCTTTACACGCAGCGGATTGAGCGTGAAAACCTGAATATCCGTAATCGGTTGAAACGACTGAACCGCAAGACTCTCGGAGACTCAAAGTCGG
CAGAAATGCATAACAGGATCATCGGTACGTTCATTGAGCGTGAGCATTACCTAGTGTGATGCTAATAACCAGATTGAATACATGACC
TTCCACCCTGTTAAAAAGCACGGGCCAGGACGCACAGGGCATCAGCGCTACCGCTGTCAGGCCTGCCGACGGAGCTTTCAACTAGAGTACGAGTATCGTG
CCTGCCACCCCGGTATGAAAGAGCAAATTGTCGATCTTGCTATGAACAATGCCGGTATTCGTGATACTGCACGGGCGTTGCATATCAGTATCAATGCGGT
CATGCGCACTTTAAAAAACTCTCGCCGCAGCGTGTAACCACGCTCCCACTGGATAACCTGCAAATTCAGCTTATCTGTGAAGTCGATGAAATGTGGTCAT
TTGTGGGCAACAAAAAGCAGCAACGTTGGCTTTGGTATGCCTGGGAGCCTCGCCTCAAACGTATTATTGCTCATGTTTTTGGTCGTCGGAGCAAAAAGAC
ATTTCGCCAATTATTGGGGTTGCTGTCAGGTTTCAATATCGTCTTCTGGTGTACCGACAACTTCAGTGCTTATGAGATGTTGCCGGATGAAAAACACATC
AGGAGCAAGCTTTACACGCAGCGGATTGAGCGTGAAAACCTGAATATCCGTAATCGGTTGAAACGACTGAACCGCAAGACTCTCGGAGACTCAAAGTCGG
CAGAAATGCATAACAGGATCATCGGTACGTTCATTGAGCGTGAGCATTACCTAGTGTGATGCTAATAACCAGATTGAATACATGACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 276 bp | 91 aa | 62 | 337 | + | No |
Description : First part of the transposase
ORF sequence :
MAKVDVKCPFCEQFHPVKKHGPGRTGHQRYRCQACRRSFQLEYEYRACHPGMKEQIVDLAMNNAGIRDTARALHISINAVMRTLKNSRRSV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 495 bp | 164 aa | 265 | 759 | + | No |
Description : Second part of the transposase
ORF sequence :
YCTGVAYQYQCGHAHFKKLSPQRVTTLPLDNLQIQLICEVDEMWSFVGNKKQQRWLWYAWEPRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNF
SAYEMLPDEKHIRSKLYTQRIERENLNIRNRLKRLNRKTLGDSKSAEMHNRIIGTFIEREHYLV
SAYEMLPDEKHIRSKLYTQRIERENLNIRNRLKRLNRKTLGDSKSAEMHNRIIGTFIEREHYLV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 698 bp | 232 aa | 62 | 759 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKVDVKCPFCEQFHPVKKHGPGRTGHQRYRCQACRRSFQLEYEYRACHPGMKEQIVDLAMNNAGIRDTARALHISINAVMRTLKKLSPQRVTTLPLDNL
QIQLICEVDEMWSFVGNKKQQRWLWYAWEPRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEMLPDEKHIRSKLYTQRIERENLNIRNRL
KRLNRKTLGDSKSAEMHNRIIGTFIEREHYLV
QIQLICEVDEMWSFVGNKKQQRWLWYAWEPRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEMLPDEKHIRSKLYTQRIERENLNIRNRL
KRLNRKTLGDSKSAEMHNRIIGTFIEREHYLV
Blast result :
Comments
ISYps7 is 91% aa similar to ISEhe5.
The third ORF is the putative oRFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative oRFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] NCBI Microbial Genomes Annotation Project (2006) Direct submission GenBank.