ISIlo12
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006512 | ND | Idiomarina loihiensis | Idiomarina loihiensis L2TR |
DNA section
IS Length : 1223 bp
Ends
IR Length : 20/26
IRL : GGGTTCTACCCAGATTTTACGGACACATCCATAAAGAGGCATAATATGCC
IRR : GGGTTCTACCCCTTTATCACGGACGGTTTAAAATCGCACTAAACTGCTGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATAACTAAAC | AGTACAGATA | 0 |
DNA sequence
GGGTTCTACCCAGATTTTACGGACACATCCATAAAGAGGCATAATATGCCCATAAGGAGTGTTCATGACCAAACGAAAAAAGTACAGCCCAGAGTTTAAA
CGTGAAGCCGTAGCGTTGACAAAACAGCCCGGTGTAAGTTGTCGCCAGGTGGCTATAGAAATCGGTATTAACCCCAATTTGCTCAGCCGTTGGCGGCGTG
AAGCCGAGCCATCCTCTAAAGAAGCTTTCAAAGGCTCAGGTAACCCCCGCGACGAAGAAATGGCGCGGCTTAAACGCGAGAATGCCCGGCTTAAAAAGGA
ACGGGATTTTTTTGCGAGAAGCGGCAACGTTCTTCGCAAAAGAGTCGTCCTGAGATATCAGGCGATAGAGCGTTGCCGCGATAAATTTCCGATACGCTTA
ATGTGTCGATGCCTGCAAGTGTCACCCAGCGGATATTACGCCTGGGCAGCACGACCGCCCAGTGCCCGTGATGTGGAAAATCAGCGCATTCTGACCCGCA
TTAAGGAGATCCACGAGGACAGTGGTGGTGTCATTGGCGCACCACGTATGCATGAAGATCTGTCTATTGAAGGTGAACGACTGAGTCTGAATCGGGTTGC
CCGCTTAATGGCCGTCAATGGCGTTCAGGGCTGGCCTCGTAAGAAAAGACGAGGCCGAAAGCGACAGGCCGCCAGGCCCGCTGGCCTTAAAAATCATCTG
GCACGTGACTTCACAGCGGCAGAGCCTGAAACTAAATGGGTCACCGATATTACCGAAGTGACAACCACCGAAGGCAAGTTGTTTCTGTGCGTTGTGCTCG
ACTTGTACAGCAAGCTGATAGTGGGCTGGTCCATGCATCACCGTCAGGATCGGTACATGGTCATTCGCGCAGCGGAAATGGCCGTCTGGCAACGAACAGG
CAATCAGCCCGTGATTTTACATTCAGATCGCGGGTCTCAATTTACCAGTGGTGATTATCAGCGTGCTCCCAAATTTAATAACCTGATCAGCAGTATGAGC
GCAGTCGGTCATTGCAGCGACAACGCTGCCTGTGAAGGATTTTTTGGAATACTGAAGCGTGAAAGAGTGAATTACCGCCGCTATCGAACAAGAGATGAAG
CACGTTCAGACATCTTTGATTATATTGAACGGTTTCATAATCCAAGAATGCGTCGTAGACTGGCAAAACAAGATCAGCAGTTTAGTGCGATTTTAAACCG
TCCGTGATAAAGGGGTAGAACCC
CGTGAAGCCGTAGCGTTGACAAAACAGCCCGGTGTAAGTTGTCGCCAGGTGGCTATAGAAATCGGTATTAACCCCAATTTGCTCAGCCGTTGGCGGCGTG
AAGCCGAGCCATCCTCTAAAGAAGCTTTCAAAGGCTCAGGTAACCCCCGCGACGAAGAAATGGCGCGGCTTAAACGCGAGAATGCCCGGCTTAAAAAGGA
ACGGGATTTTTTTGCGAGAAGCGGCAACGTTCTTCGCAAAAGAGTCGTCCTGAGATATCAGGCGATAGAGCGTTGCCGCGATAAATTTCCGATACGCTTA
ATGTGTCGATGCCTGCAAGTGTCACCCAGCGGATATTACGCCTGGGCAGCACGACCGCCCAGTGCCCGTGATGTGGAAAATCAGCGCATTCTGACCCGCA
TTAAGGAGATCCACGAGGACAGTGGTGGTGTCATTGGCGCACCACGTATGCATGAAGATCTGTCTATTGAAGGTGAACGACTGAGTCTGAATCGGGTTGC
CCGCTTAATGGCCGTCAATGGCGTTCAGGGCTGGCCTCGTAAGAAAAGACGAGGCCGAAAGCGACAGGCCGCCAGGCCCGCTGGCCTTAAAAATCATCTG
GCACGTGACTTCACAGCGGCAGAGCCTGAAACTAAATGGGTCACCGATATTACCGAAGTGACAACCACCGAAGGCAAGTTGTTTCTGTGCGTTGTGCTCG
ACTTGTACAGCAAGCTGATAGTGGGCTGGTCCATGCATCACCGTCAGGATCGGTACATGGTCATTCGCGCAGCGGAAATGGCCGTCTGGCAACGAACAGG
CAATCAGCCCGTGATTTTACATTCAGATCGCGGGTCTCAATTTACCAGTGGTGATTATCAGCGTGCTCCCAAATTTAATAACCTGATCAGCAGTATGAGC
GCAGTCGGTCATTGCAGCGACAACGCTGCCTGTGAAGGATTTTTTGGAATACTGAAGCGTGAAAGAGTGAATTACCGCCGCTATCGAACAAGAGATGAAG
CACGTTCAGACATCTTTGATTATATTGAACGGTTTCATAATCCAAGAATGCGTCGTAGACTGGCAAAACAAGATCAGCAGTTTAGTGCGATTTTAAACCG
TCCGTGATAAAGGGGTAGAACCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1143 bp | 380 aa | 65 | 1207 | + | No |
Chemistry : DDE
ORF sequence :
MTKRKKYSPEFKREAVALTKQPGVSCRQVAIEIGINPNLLSRWRREAEPSSKEAFKGSGNPRDEEMARLKRENARLKKERDFFARSGNVLRKRVVLRYQA
IERCRDKFPIRLMCRCLQVSPSGYYAWAARPPSARDVENQRILTRIKEIHEDSGGVIGAPRMHEDLSIEGERLSLNRVARLMAVNGVQGWPRKKRRGRKR
QAARPAGLKNHLARDFTAAEPETKWVTDITEVTTTEGKLFLCVVLDLYSKLIVGWSMHHRQDRYMVIRAAEMAVWQRTGNQPVILHSDRGSQFTSGDYQR
APKFNNLISSMSAVGHCSDNAACEGFFGILKRERVNYRRYRTRDEARSDIFDYIERFHNPRMRRRLAKQDQQFSAILNRP
IERCRDKFPIRLMCRCLQVSPSGYYAWAARPPSARDVENQRILTRIKEIHEDSGGVIGAPRMHEDLSIEGERLSLNRVARLMAVNGVQGWPRKKRRGRKR
QAARPAGLKNHLARDFTAAEPETKWVTDITEVTTTEGKLFLCVVLDLYSKLIVGWSMHHRQDRYMVIRAAEMAVWQRTGNQPVILHSDRGSQFTSGDYQR
APKFNNLISSMSAVGHCSDNAACEGFFGILKRERVNYRRYRTRDEARSDIFDYIERFHNPRMRRRLAKQDQQFSAILNRP
Blast result :
Comments
ISIlo12 is 61% (ORFA) aa similar to ISCps2. There is no -1 programmed frameshift.
References
1] Hou,S., Saw,J.H., Lee,K.S., Freitas,T.A., Belisle,C., Kawarabayasi,Y., Donachie,S.P., Pikina,A., Galperin,M.Y., Koonin,E.V., Makarova,K.S., Omelchenko,M.V., Sorokin,A., Wolf,Y.I., Li,Q.X., Keum,Y.S., Campbell,S., Denery,J., Aizawa,S.I., Shibata,S., Malahoff,A. and Alam,M. (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (52), 18036-18041.