ISfinder Mirror Site

ISAusp1

Family IS1595
Group ISNha5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAPJ01000002	ND	Aurantimonas sp.	Aurantimonas sp. SI85-9A1

DNA section

IS Length : 3418 bp

Ends

IR Length : 25/28

IRL : CGCCCTTATGTAGCAAACGCACCAAGGACCGCACGATGAGACTGGCAGAG
IRR : CGCCCTTATGTAGCAGATGCACCATGGAAAAGGTAGGCGGAACAGCCGAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTCGCGCATCCCATGTC	CACAGATG	AATGGCGTCCGGCG	8

DNA sequence

CGCCCTTATGTAGCAAACGCACCAAGGACCGCACGATGAGACTGGCAGAGACTCCACAACTCAAAGAAGGTCGGGTTAGTTGGGCGCCATGCGTTTCATC
TGGTTCGTCCTCAAAATGCTTACCTATGGGTGGACGCTCTTCGGCGCCGCGAGCGCCGTGGTCGGAGTCGCCGGTATACCCGACGATATCGCGACATGGA
CAAGGTGGATCGACGCTATGGTGAGCGATCCCACAGTGATCTGGCTTGCGGAGTATGCCGTGGCCATCGCATCCTTCATAAATCAGCTATGGGTTCGTGT
GGCTCTCGTCATAATAGGATGCGTCATCTTCATTTGGCCGGCTCGGGAGTTCTGGCGCGCCCGTCACCGACTGATTTTTTGGGTGAGGAATAAATTGGAA
GATAAGGTCTGGATTACCCGTGAGGACGCCATCGAACTGGTATCCAACTCCGATTGGGCTTTAGCGCGAGAGCCAACAATCACGATATTTGACCGCTTAA
ACTCAAATATGCTTCGGCAAGGGATGACCCCAGCCGAGAAGCTGAACTCTAAATTTCTCCGTTTGTGCGAGATGATGCTTGAACAGTTTGAGGATCATTA
TGACGGAGTCACCAAGGAAGACGACGGCCATAAACTAATCTCTGAAGGTGATCTTCGCGACCATCTGAAACGAGCGTACGATAAGGACGTGATCGAAGAA
TTTGGGAAGCTGCCAAACCCTTATTAATCTAGGCGTATCGCGCTCGACCCGATAGCTTCGTAGCACGCAGCCATGAAGGCCGCTGTGAACTTGCCGCGCG
AGAGCTTGTTGCGGATGTTCACCTCCTTCTCCTCGACACCTATCGCCGCGAGCTTCTCAACGAGCTGGGCATAGGTCACGCCCTTTCGTTTCAATTCGGC
CTTAAGGATATTCGCTACGCGGGTTTCGTAATCCGTATGCTCTGCCATCGACGTATCGTCTCCGATAAAAAATGTATCGCAAACGATAGATATGCCCTTG
CATCCAGTACGTCAATGTACTACATCCATTACATAGGTAATGGAAACGATGACAATGGCACAGCACTTTCTTCTCTCCGCAAAGGCCCGCACCCTTTCTA
TCCGCGCCATCTACAAGGCCGGCGAGGAAAAGGCTTACGAGCAGTTCCGTTCTATCCGCTGGGCCTCCACCAACGGCCAGGCTGTTTGCCCCCGCTGTGG
CTGCGTCGATACCTACGAGATCACCACCCGCCGCCGCTTCAAGTGCGCTGCCTGCCATCACCAGTTCTCGGTGACGAGCGGGACGATCTTCCATTCCCGC
AAGATGGGCTTCACGGATCTCCTGGCCGCGATCGCGCTGTTCGTGAACGCCGTTAAAGGCATGTCTGCCCTGCAGATGAGCCGCGATCTCGATTGCCAGT
ACAAGACGGCCTTCGTTCTCTGCCACAAGCTGCGCGAGGCGATGGCTAACGAGACGGCAGGCTTCACGCTGGACGGAGAAGTCGAGATCGACGGCGCCTA
TTTCGGTGGGTCCATCAAGCCGCAGAACCGCAAGGAAGACCGGATCGACCGTCGCTTGAAGGAACACCAGACCGGCACCCGCCGCGTCGTCATCGGGCTT
CGCCAGCGTGCGGGACGCACCCTGACCTTCGTCCGCAACTTTGAGCATGAAGGCGTGCAGATCGCGCGCGATCTCGTGGCTTCGGCTTCGACCGTGTTCG
CTGACGAAGCGACCCATTGGGACGTGTTGGAAGCCTTCTTCCCCACCGAGCGCATCAATCACTCGGAAGCCTACAGTGAGGGTCACGGCAAGCACACGAA
CTGGGTGGAAAGCTTCTTCTCCCGCCTGCGCCGTATGGTCGCCGGTCAGCACCACGGCGTATCGCCGAAGTACCTCGCAGCCTACGCCTCAAACGCCGCA
TGGTGCGAAGATCACCGTCGTCGCGACAACGGCTGCAACGCCGTTGCGTTGGTCGGCAACGCGATGCTCTCGCCGGTCAGCCGGTCGTGGTGCGGGTATT
GGCAAAAATAGCCTGTCAATGAATTGTTAATTGACTGGTGCGTAGATTACATACATATGGTGCATATGAGCGAGCCCCAGCAACACACCGTCTCTGAGCT
AAACACCTTTCGCCGGCACGCGGAGGTAGCTGGAATGTCACAGGATGACATCGACCGACTGACTGACTACCTATCAAAAAATCCCGCTGCCGGCGACGAG
ATAAAAGGTACGGGTGGATGTCGAAAGGTGCGGTTCGCTCTCGCGCGCAACACCAAGGGCAAGAGTGGCGGAGTCCGCACGATCACTCTATTCACAGGGC
CAAATCTGCCTGTCTTCCTGATAACGGTTTTTGCGAAATCGAAAAAGGTGACGCTTTCCAAGGCGGAACGTAATGGCCTGAAGAAGCTTTCAGATCTCAT
AGTCGCCGACTATTCGAGACGAGTTATGCCGATCGCGGCAGGAGATACAGGATGAGCAAGGAAGTTTTCAACCAAATAGCCGAAGGTTTGGAAGAAGCTC
TTTCCGTCACGCGTGGCGAGTCTTCGCCTTATCGCCTTTACGTTCCTTCGGAAGTTGATGTTAAGGCCATTAGGTCCAAGACCGGCTTTTCTCAGAAGGA
TTTCGCTTCGACGTTTGGGTTCGGGCTCGACCAGCTTAAGCAGTGGGAGCAGGGAAGATCGCGCCCCGTACAGGCGCACCGTGCCTATCTCCTGCTTATC
AACGATAGGCCAAAGGAGATGGTCGAAGCCCTGCGCGAGATCAACATGCATAAGTTTGATGATGGGCGCCGAAGGGCAGTAGGCTAGCACACCAATTGAT
TGACCCCGCTTCGGCGGGGTTTTTCGTTGTGAAGTTAATTTCACTGACCGCCATACGTCGTATAAAGTACGGCAGCCATACCTCAGCCCTGTAGCCGCCA
CACCCTCGCGTCCCCATCCCAGCCATCGCGCACCACCCCGCCTTGCCGCTCCAGCCTGTGCAGCATCTTCCGCACGGCTGACAGGATGGCTTTCGGGTCC
GGCTCGTGGTCGTCGTGCAGCTTGAACAGGGGCGCTACCGCCTCCGCAAGCTGGGTGGTCGTGGCGGCGCCAGTAGAGCGCAGCGCGGCGAAGATGGCCC
GATGCCGCGCTAGAACGTCCGCCCGCTTTGCGCGGGGCGTGGTGGCCCGCTGCAGGGGCTTCACCTTGTGACCGTCCGTTACGCCGAACATCGCCAACAC
GGCGTCTAGATGGCCTAGATCGCGATCCACTGCGGCCAGGTGCTCGACGGCGCGGACGCGCTCGCCTGCCATCTCGGCGCGTTTCGTAAGCAGCCCAGCT
ATGGTAAGCTTGTAGCTGTCGGTTCGGGTGGCGCGGTGCTTTCGTTCCATGCGCCCATTATCGCGCTAGTCGGCTGTTCCGCCTACCTTTTCCATGGTGC
ATCTGCTACATAAGGGCG

Protein section

ORF number : 5

ORF 1

Length		Begin	End	Strand	Fusion ORF
648 bp	215 aa	80	727	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGAMRFIWFVLKMLTYGWTLFGAASAVVGVAGIPDDIATWTRWIDAMVSDPTVIWLAEYAVAIASFINQLWVRVALVIIGCVIFIWPAREFWRARHRLIF
WVRNKLEDKVWITREDAIELVSNSDWALAREPTITIFDRLNSNMLRQGMTPAEKLNSKFLRLCEMMLEQFEDHYDGVTKEDDGHKLISEGDLRDHLKRAY
DKDVIEEFGKLPNPY

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
225 bp	74 aa	724	948	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MAEHTDYETRVANILKAELKRKGVTYAQLVEKLAAIGVEEKEVNIRNKLSRGKFTAAFMAACYEAIGSSAIRLD

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
972 bp	323 aa	1040	2011	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

METMTMAQHFLLSAKARTLSIRAIYKAGEEKAYEQFRSIRWASTNGQAVCPRCGCVDTYEITTRRRFKCAACHHQFSVTSGTIFHSRKMGFTDLLAAIAL
FVNAVKGMSALQMSRDLDCQYKTAFVLCHKLREAMANETAGFTLDGEVEIDGAYFGGSIKPQNRKEDRIDRRLKEHQTGTRRVVIGLRQRAGRTLTFVRN
FEHEGVQIARDLVASASTVFADEATHWDVLEAFFPTERINHSEAYSEGHGKHTNWVESFFSRLRRMVAGQHHGVSPKYLAAYASNAAWCEDHRRRDNGCN
AVALVGNAMLSPVSRSWCGYWQK

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
432 bp	143 aa	2024	2455	+	No

ORF function : Passenger Gene
Annotation : RelE-like cytotoxic translational repressor of toxin-antitoxin stability systemDescription :

ORF sequence :

MLIDWCVDYIHMVHMSEPQQHTVSELNTFRRHAEVAGMSQDDIDRLTDYLSKNPAAGDEIKGTGGCRKVRFALARNTKGKSGGVRTITLFTGPNLPVFLI
TVFAKSKKVTLSKAERNGLKKLSDLIVADYSRRVMPIAAGDTG

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
468 bp	155 aa	3350	2883	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MERKHRATRTDSYKLTIAGLLTKRAEMAGERVRAVEHLAAVDRDLGHLDAVLAMFGVTDGHKVKPLQRATTPRAKRADVLARHRAIFAALRSTGAATTTQ
LAEAVAPLFKLHDDHEPDPKAILSAVRKMLHRLERQGGVVRDGWDGDARVWRLQG

Blast result :

Comments

ISAusp1 is 47% aa similar to ISPpr5.
The third ORF is the transposase.

References

1] Dick,G.J., Podell,S., Johnson,H.A., Bencheikh-Latmani,R., McCarthy,J.K., Rivera Espinoza,Y., Clement,B.G., Gaasterland,T., Tebo,B.M., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.