ISAusp1

  • Family IS1595
  • Group ISNha5
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AAPJ01000002 ND Aurantimonas sp.
Aurantimonas sp. SI85-9A1
DNA section
IS Length : 3418 bp

Ends


IR Length : 25/28

IRL : CGCCCTTATGTAGCAAACGCACCAAGGACCGCACGATGAGACTGGCAGAG
IRR : CGCCCTTATGTAGCAGATGCACCATGGAAAAGGTAGGCGGAACAGCCGAC

Insertion site


Left flankDirect repeatRight flankDR Length
GTCGCGCATCCCATGTCCACAGATGAATGGCGTCCGGCG8

DNA sequence

CGCCCTTATGTAGCAAACGCACCAAGGACCGCACGATGAGACTGGCAGAGACTCCACAACTCAAAGAAGGTCGGGTTAGTTGGGCGCCATGCGTTTCATC
TGGTTCGTCCTCAAAATGCTTACCTATGGGTGGACGCTCTTCGGCGCCGCGAGCGCCGTGGTCGGAGTCGCCGGTATACCCGACGATATCGCGACATGGA
CAAGGTGGATCGACGCTATGGTGAGCGATCCCACAGTGATCTGGCTTGCGGAGTATGCCGTGGCCATCGCATCCTTCATAAATCAGCTATGGGTTCGTGT
GGCTCTCGTCATAATAGGATGCGTCATCTTCATTTGGCCGGCTCGGGAGTTCTGGCGCGCCCGTCACCGACTGATTTTTTGGGTGAGGAATAAATTGGAA
GATAAGGTCTGGATTACCCGTGAGGACGCCATCGAACTGGTATCCAACTCCGATTGGGCTTTAGCGCGAGAGCCAACAATCACGATATTTGACCGCTTAA
ACTCAAATATGCTTCGGCAAGGGATGACCCCAGCCGAGAAGCTGAACTCTAAATTTCTCCGTTTGTGCGAGATGATGCTTGAACAGTTTGAGGATCATTA
TGACGGAGTCACCAAGGAAGACGACGGCCATAAACTAATCTCTGAAGGTGATCTTCGCGACCATCTGAAACGAGCGTACGATAAGGACGTGATCGAAGAA
TTTGGGAAGCTGCCAAACCCTTATTAATCTAGGCGTATCGCGCTCGACCCGATAGCTTCGTAGCACGCAGCCATGAAGGCCGCTGTGAACTTGCCGCGCG
AGAGCTTGTTGCGGATGTTCACCTCCTTCTCCTCGACACCTATCGCCGCGAGCTTCTCAACGAGCTGGGCATAGGTCACGCCCTTTCGTTTCAATTCGGC
CTTAAGGATATTCGCTACGCGGGTTTCGTAATCCGTATGCTCTGCCATCGACGTATCGTCTCCGATAAAAAATGTATCGCAAACGATAGATATGCCCTTG
CATCCAGTACGTCAATGTACTACATCCATTACATAGGTAATGGAAACGATGACAATGGCACAGCACTTTCTTCTCTCCGCAAAGGCCCGCACCCTTTCTA
TCCGCGCCATCTACAAGGCCGGCGAGGAAAAGGCTTACGAGCAGTTCCGTTCTATCCGCTGGGCCTCCACCAACGGCCAGGCTGTTTGCCCCCGCTGTGG
CTGCGTCGATACCTACGAGATCACCACCCGCCGCCGCTTCAAGTGCGCTGCCTGCCATCACCAGTTCTCGGTGACGAGCGGGACGATCTTCCATTCCCGC
AAGATGGGCTTCACGGATCTCCTGGCCGCGATCGCGCTGTTCGTGAACGCCGTTAAAGGCATGTCTGCCCTGCAGATGAGCCGCGATCTCGATTGCCAGT
ACAAGACGGCCTTCGTTCTCTGCCACAAGCTGCGCGAGGCGATGGCTAACGAGACGGCAGGCTTCACGCTGGACGGAGAAGTCGAGATCGACGGCGCCTA
TTTCGGTGGGTCCATCAAGCCGCAGAACCGCAAGGAAGACCGGATCGACCGTCGCTTGAAGGAACACCAGACCGGCACCCGCCGCGTCGTCATCGGGCTT
CGCCAGCGTGCGGGACGCACCCTGACCTTCGTCCGCAACTTTGAGCATGAAGGCGTGCAGATCGCGCGCGATCTCGTGGCTTCGGCTTCGACCGTGTTCG
CTGACGAAGCGACCCATTGGGACGTGTTGGAAGCCTTCTTCCCCACCGAGCGCATCAATCACTCGGAAGCCTACAGTGAGGGTCACGGCAAGCACACGAA
CTGGGTGGAAAGCTTCTTCTCCCGCCTGCGCCGTATGGTCGCCGGTCAGCACCACGGCGTATCGCCGAAGTACCTCGCAGCCTACGCCTCAAACGCCGCA
TGGTGCGAAGATCACCGTCGTCGCGACAACGGCTGCAACGCCGTTGCGTTGGTCGGCAACGCGATGCTCTCGCCGGTCAGCCGGTCGTGGTGCGGGTATT
GGCAAAAATAGCCTGTCAATGAATTGTTAATTGACTGGTGCGTAGATTACATACATATGGTGCATATGAGCGAGCCCCAGCAACACACCGTCTCTGAGCT
AAACACCTTTCGCCGGCACGCGGAGGTAGCTGGAATGTCACAGGATGACATCGACCGACTGACTGACTACCTATCAAAAAATCCCGCTGCCGGCGACGAG
ATAAAAGGTACGGGTGGATGTCGAAAGGTGCGGTTCGCTCTCGCGCGCAACACCAAGGGCAAGAGTGGCGGAGTCCGCACGATCACTCTATTCACAGGGC
CAAATCTGCCTGTCTTCCTGATAACGGTTTTTGCGAAATCGAAAAAGGTGACGCTTTCCAAGGCGGAACGTAATGGCCTGAAGAAGCTTTCAGATCTCAT
AGTCGCCGACTATTCGAGACGAGTTATGCCGATCGCGGCAGGAGATACAGGATGAGCAAGGAAGTTTTCAACCAAATAGCCGAAGGTTTGGAAGAAGCTC
TTTCCGTCACGCGTGGCGAGTCTTCGCCTTATCGCCTTTACGTTCCTTCGGAAGTTGATGTTAAGGCCATTAGGTCCAAGACCGGCTTTTCTCAGAAGGA
TTTCGCTTCGACGTTTGGGTTCGGGCTCGACCAGCTTAAGCAGTGGGAGCAGGGAAGATCGCGCCCCGTACAGGCGCACCGTGCCTATCTCCTGCTTATC
AACGATAGGCCAAAGGAGATGGTCGAAGCCCTGCGCGAGATCAACATGCATAAGTTTGATGATGGGCGCCGAAGGGCAGTAGGCTAGCACACCAATTGAT
TGACCCCGCTTCGGCGGGGTTTTTCGTTGTGAAGTTAATTTCACTGACCGCCATACGTCGTATAAAGTACGGCAGCCATACCTCAGCCCTGTAGCCGCCA
CACCCTCGCGTCCCCATCCCAGCCATCGCGCACCACCCCGCCTTGCCGCTCCAGCCTGTGCAGCATCTTCCGCACGGCTGACAGGATGGCTTTCGGGTCC
GGCTCGTGGTCGTCGTGCAGCTTGAACAGGGGCGCTACCGCCTCCGCAAGCTGGGTGGTCGTGGCGGCGCCAGTAGAGCGCAGCGCGGCGAAGATGGCCC
GATGCCGCGCTAGAACGTCCGCCCGCTTTGCGCGGGGCGTGGTGGCCCGCTGCAGGGGCTTCACCTTGTGACCGTCCGTTACGCCGAACATCGCCAACAC
GGCGTCTAGATGGCCTAGATCGCGATCCACTGCGGCCAGGTGCTCGACGGCGCGGACGCGCTCGCCTGCCATCTCGGCGCGTTTCGTAAGCAGCCCAGCT
ATGGTAAGCTTGTAGCTGTCGGTTCGGGTGGCGCGGTGCTTTCGTTCCATGCGCCCATTATCGCGCTAGTCGGCTGTTCCGCCTACCTTTTCCATGGTGC
ATCTGCTACATAAGGGCG
Protein section
ORF number : 5

 

ORF 1
LengthBeginEndStrandFusion ORF
648 bp215 aa80727+No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGAMRFIWFVLKMLTYGWTLFGAASAVVGVAGIPDDIATWTRWIDAMVSDPTVIWLAEYAVAIASFINQLWVRVALVIIGCVIFIWPAREFWRARHRLIF
WVRNKLEDKVWITREDAIELVSNSDWALAREPTITIFDRLNSNMLRQGMTPAEKLNSKFLRLCEMMLEQFEDHYDGVTKEDDGHKLISEGDLRDHLKRAY
DKDVIEEFGKLPNPY

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
225 bp74 aa724948-No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MAEHTDYETRVANILKAELKRKGVTYAQLVEKLAAIGVEEKEVNIRNKLSRGKFTAAFMAACYEAIGSSAIRLD

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
972 bp323 aa10402011+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

METMTMAQHFLLSAKARTLSIRAIYKAGEEKAYEQFRSIRWASTNGQAVCPRCGCVDTYEITTRRRFKCAACHHQFSVTSGTIFHSRKMGFTDLLAAIAL
FVNAVKGMSALQMSRDLDCQYKTAFVLCHKLREAMANETAGFTLDGEVEIDGAYFGGSIKPQNRKEDRIDRRLKEHQTGTRRVVIGLRQRAGRTLTFVRN
FEHEGVQIARDLVASASTVFADEATHWDVLEAFFPTERINHSEAYSEGHGKHTNWVESFFSRLRRMVAGQHHGVSPKYLAAYASNAAWCEDHRRRDNGCN
AVALVGNAMLSPVSRSWCGYWQK

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
432 bp143 aa20242455+No
ORF function : Passenger Gene
Annotation : RelE-like cytotoxic translational repressor of toxin-antitoxin stability systemDescription :

ORF sequence :

MLIDWCVDYIHMVHMSEPQQHTVSELNTFRRHAEVAGMSQDDIDRLTDYLSKNPAAGDEIKGTGGCRKVRFALARNTKGKSGGVRTITLFTGPNLPVFLI
TVFAKSKKVTLSKAERNGLKKLSDLIVADYSRRVMPIAAGDTG

 

Blast result :
ORF 5
LengthBeginEndStrandFusion ORF
468 bp155 aa33502883-No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MERKHRATRTDSYKLTIAGLLTKRAEMAGERVRAVEHLAAVDRDLGHLDAVLAMFGVTDGHKVKPLQRATTPRAKRADVLARHRAIFAALRSTGAATTTQ
LAEAVAPLFKLHDDHEPDPKAILSAVRKMLHRLERQGGVVRDGWDGDARVWRLQG

 

Blast result :
Comments
ISAusp1 is 47% aa similar to ISPpr5.
The third ORF is the transposase.
References
1] Dick,G.J., Podell,S., Johnson,H.A., Bencheikh-Latmani,R., McCarthy,J.K., Rivera Espinoza,Y., Clement,B.G., Gaasterland,T., Tebo,B.M., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.