ISSwo1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008346 | ND | Syntrophomonas wolfei | Syntrophomonas wolfei subsp. wolfei |
DNA section
IS Length : 1267 bp
Ends
IR Length : 30/46
IRL : TGGATAGGCTATACTAAATAGGACAGTTTTTCTCCGAACATGATATAACA
IRR : TGGATTGGCAACTCCTTTTTAGACAAAATTACTCCGAACAAGAAATTACT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCATAGAGT | CTATTAGAAC | 0 |
DNA sequence
TGGATAGGCTATACTAAATAGGACAGTTTTTCTCCGAACATGATATAACAAAATTATAGGAGGATAACAGTATGGCAAAAAAACATTATGAGGAAAATTT
TAAGAAGCAAATTGTAAAAATATATAATCAAGGTAATCATAGCTATAGGGAATTAAGTGAGCAATATGATATAGCTCCATCAACTGTAAGACAATGGGTT
ATGAGATATAATAATACGCAATCCTTTCATGCAGAAGATAATAGATCAGAAGAAGAAAAGGAACTAATTGAACTGAGAAAAAAATTAAAACAGCTTGAAA
TGGAAAATGATATTTTAAAGCAAGCGGCACTACTACTAGGGAAAAGGTAGACCTCATTATTTCTAATAGAGAGAAATACAGAATTAGTGCCATGTGCGAA
TTTTTAGAGGTCACACGTAGCTTAGTATATTATCATTTAAATAAAGAACCTAGAGTTTTCTCAAGGGAAGAAGAAATTATCCAAGAACACATAAAAGAGA
TATTTAGATTTAGCAGAAATAATTATGGAACAAGAAAAATCAAAGAAGAATTAAAGAAGTTAGGTTATCAGGTATCAAGAAAAAGGATAGCAAGACTTAT
GAGAAAGAATTGCCTAGTATCTAACTATACAGTGGCTCAATACAAGGTTCATAAGAGTACTTGTAACCAAGCTGAAACTCCTAATGTTGTAGATAGAGAC
TTTGATAATAGAGAGAAATTAGAAGTCATTGTAAGTGATTTAACCTATGTTAGAGTGGGTTGTAAATGGAATTATGTTTGCAACTTAATAGACCTTCACA
ATCGTGAAATTATAGGTTCAGCTGCAGGAGAAAAGAAAGATGCTAGACTGGTAGAAAGAGTTCTTTTAAGTATACCCTATTCACTGAATGATATAAAAAT
TTTTCATAGTGATCGGGGAAATGAGTATGATAACAAGATTATAGATGATGTTTTAGAAGCATTTGAAATAGAGAGATCTTTAAGTAATAAAGGTAATCCT
TACGACAATGCAGTAAGTGAGGCAGTAAATAAAATAATGAAAACTGAATTTATATATAAGAACAAATTTAAGACATTAGAAGAGTTGCAGTTAGAATTAG
CAGAGTATATTTATTGGTATAATAATCTGAGAATTCATGGTTCTTTAGGTTATTTAACACCAGTGAAATATAGGGAGTTAAGCACACTTAAGTTAGCAGG
ATAAGAACTGTAGTAAAAGTAATTTCTTGTTCGGAGTAATTTTGTCTAAAAAGGAGTTGCCAATCCA
TAAGAAGCAAATTGTAAAAATATATAATCAAGGTAATCATAGCTATAGGGAATTAAGTGAGCAATATGATATAGCTCCATCAACTGTAAGACAATGGGTT
ATGAGATATAATAATACGCAATCCTTTCATGCAGAAGATAATAGATCAGAAGAAGAAAAGGAACTAATTGAACTGAGAAAAAAATTAAAACAGCTTGAAA
TGGAAAATGATATTTTAAAGCAAGCGGCACTACTACTAGGGAAAAGGTAGACCTCATTATTTCTAATAGAGAGAAATACAGAATTAGTGCCATGTGCGAA
TTTTTAGAGGTCACACGTAGCTTAGTATATTATCATTTAAATAAAGAACCTAGAGTTTTCTCAAGGGAAGAAGAAATTATCCAAGAACACATAAAAGAGA
TATTTAGATTTAGCAGAAATAATTATGGAACAAGAAAAATCAAAGAAGAATTAAAGAAGTTAGGTTATCAGGTATCAAGAAAAAGGATAGCAAGACTTAT
GAGAAAGAATTGCCTAGTATCTAACTATACAGTGGCTCAATACAAGGTTCATAAGAGTACTTGTAACCAAGCTGAAACTCCTAATGTTGTAGATAGAGAC
TTTGATAATAGAGAGAAATTAGAAGTCATTGTAAGTGATTTAACCTATGTTAGAGTGGGTTGTAAATGGAATTATGTTTGCAACTTAATAGACCTTCACA
ATCGTGAAATTATAGGTTCAGCTGCAGGAGAAAAGAAAGATGCTAGACTGGTAGAAAGAGTTCTTTTAAGTATACCCTATTCACTGAATGATATAAAAAT
TTTTCATAGTGATCGGGGAAATGAGTATGATAACAAGATTATAGATGATGTTTTAGAAGCATTTGAAATAGAGAGATCTTTAAGTAATAAAGGTAATCCT
TACGACAATGCAGTAAGTGAGGCAGTAAATAAAATAATGAAAACTGAATTTATATATAAGAACAAATTTAAGACATTAGAAGAGTTGCAGTTAGAATTAG
CAGAGTATATTTATTGGTATAATAATCTGAGAATTCATGGTTCTTTAGGTTATTTAACACCAGTGAAATATAGGGAGTTAAGCACACTTAAGTTAGCAGG
ATAAGAACTGTAGTAAAAGTAATTTCTTGTTCGGAGTAATTTTGTCTAAAAAGGAGTTGCCAATCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 72 | 350 | + | No |
Description : Second part of the transposase
ORF sequence :
MAKKHYEENFKKQIVKIYNQGNHSYRELSEQYDIAPSTVRQWVMRYNNTQSFHAEDNRSEEEKELIELRKKLKQLEMENDILKQAALLLGKR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 894 bp | 297 aa | 311 | 1204 | + | No |
Description : Second part of the transposase
ORF sequence :
YFKASGTTTREKVDLIISNREKYRISAMCEFLEVTRSLVYYHLNKEPRVFSREEEIIQEHIKEIFRFSRNNYGTRKIKEELKKLGYQVSRKRIARLMRKN
CLVSNYTVAQYKVHKSTCNQAETPNVVDRDFDNREKLEVIVSDLTYVRVGCKWNYVCNLIDLHNREIIGSAAGEKKDARLVERVLLSIPYSLNDIKIFHS
DRGNEYDNKIIDDVLEAFEIERSLSNKGNPYDNAVSEAVNKIMKTEFIYKNKFKTLEELQLELAEYIYWYNNLRIHGSLGYLTPVKYRELSTLKLAG
CLVSNYTVAQYKVHKSTCNQAETPNVVDRDFDNREKLEVIVSDLTYVRVGCKWNYVCNLIDLHNREIIGSAAGEKKDARLVERVLLSIPYSLNDIKIFHS
DRGNEYDNKIIDDVLEAFEIERSLSNKGNPYDNAVSEAVNKIMKTEFIYKNKFKTLEELQLELAEYIYWYNNLRIHGSLGYLTPVKYRELSTLKLAG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1133 bp | 367 aa | 72 | 1204 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKKHYEENFKKQIVKIYNQGNHSYRELSEQYDIAPSTVRQWVMRYNNTQSFHAEDNRSEEEKELIELRKKLKASGTTTREKVDLIISNREKYRISAMCE
FLEVTRSLVYYHLNKEPRVFSREEEIIQEHIKEIFRFSRNNYGTRKIKEELKKLGYQVSRKRIARLMRKNCLVSNYTVAQYKVHKSTCNQAETPNVVDRD
FDNREKLEVIVSDLTYVRVGCKWNYVCNLIDLHNREIIGSAAGEKKDARLVERVLLSIPYSLNDIKIFHSDRGNEYDNKIIDDVLEAFEIERSLSNKGNP
YDNAVSEAVNKIMKTEFIYKNKFKTLEELQLELAEYIYWYNNLRIHGSLGYLTPVKYRELSTLKLAG
FLEVTRSLVYYHLNKEPRVFSREEEIIQEHIKEIFRFSRNNYGTRKIKEELKKLGYQVSRKRIARLMRKNCLVSNYTVAQYKVHKSTCNQAETPNVVDRD
FDNREKLEVIVSDLTYVRVGCKWNYVCNLIDLHNREIIGSAAGEKKDARLVERVLLSIPYSLNDIKIFHSDRGNEYDNKIIDDVLEAFEIERSLSNKGNP
YDNAVSEAVNKIMKTEFIYKNKFKTLEELQLELAEYIYWYNNLRIHGSLGYLTPVKYRELSTLKLAG
Blast result :
Comments
ISSwo1 is 74% (ORFA) aa similar to ISBlo9 and 73% (ORFB) aa similar to ISClsp1. There is no -1 programmed frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Sims,D., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Boone,D.R., Brockman,F., Culley,D., Ferry,J., Gunsalus,R., McInerney,M.J., Morrison,M., Plugge,C., Rohlin,L., Scholten,J., Sieber,J., Stams,A.J.M. and Richardson,P. (2006) Direct submission Genbank.