ISErh6
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AP012027 | ND | Erysipelothrix rhusiopathiae | Erysipelothrix rhusiopathiae SY1027 Erysipelothrix rhusiopathiae Fujisawa |
DNA section
IS Length : 1240 bp
Ends
IR Length : 24/32
IRL : TGGTATAGTGAATTTGTAACACCTGTGTATAATCTAGTACAATGGAAGAA
IRR : TGGTCAAGCGTTTTTGGAACACTAGTGTATAAATAGATCTAAGTGTTTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAAGTGTAA | CTATAGACAA | 0 |
DNA sequence
TGGTATAGTGAATTTGTAACACCTGTGTATAATCTAGTACAATGGAAGAAACAGGAGAAAACATATGAAACGTTACAGTACAGAAATGAAGAAGAAAATC
GTTACAGCTAATATCCAAGATGGACGTACCATTGCAAGCCTAGCCACTGAATATGGTGTATCCACTGCATCCATTAATAACTGGATTCGTGATTACCGCA
AAGAATGCCAAGCAACACCCGAAGCAATGAGTGAACTTGAGTTAATGGAAGAGAATCGTAAACTCAAACAAAAACTCGCTGAATTCGAAAAGAAAATGAC
TTTTTAAAAAAGGCAGCAGCATTCTTCGCGAAGGAAACAGGCAAGTAATCTACAAATTTATTGATCAGCATAAGAGCCACTTCGGCTTACGTTGGTTGTT
AAAACGATGTGGTGTTTATGCCAACGGCTATTATAACTATCTCAGAAATACAAAACGGGATTATCATCAAAGCAAGCAAGCTATCAAAAATAAAATCAAA
GATATCTTTCATTCCACTGGCGGCATTGTATGTCATCGTGGAATGGTCATATTCTTAGCTCGTGATGACATTAAGCTCAGTAAGACTACCGTTCATAAAT
ATATGAATAAAGAGCTACAACTCTACTGTATCCCCGCTAAGAAGCGCCCACGATACGTTGAAGGAACGAAACATGAGATATTCCCCAATCACTTATCGCA
AGACTTCGATATTGAAGAAAAGAACACAGTTTGGTGTACCGACTTTACTTATATCAAAGGGTGGGATGGCAAGTTCCAATACAACTGCAGTATTTTAGAC
CTTTCGGATCGGAGTATTGTCGCCACTAAAACAAGTCGAATGATGACAAGTGAACTAGCCAAAGAAACTTTAGAAACAGCACTATCATCACAGAAGAAAC
GACCTAAAGGATTGATTCTTCATAGTGATCAAGGCAGCCAATTCGCATCATATGATTTCACAGAATACTGTCTAAAAAACAATATAAAGCAGAGTATGAG
TAAAGCCGGATGTCCCTATGATAACGCAGTGATGGAACGATTTTTTAGAACCATGAAGTTTGAACTCATCAATCGATTCCAATTCGACAATGACAAGCAA
CTTGAAACTGCAATATCAGAATATGTATTTGGTTGGTATAACCAGGTAAGACCGCATGCATCAAATGGATATAAAACCCCAAACGAAATTAGAAACACTT
AGATCTATTTATACACTAGTGTTCCAAAAACGCTTGACCA
GTTACAGCTAATATCCAAGATGGACGTACCATTGCAAGCCTAGCCACTGAATATGGTGTATCCACTGCATCCATTAATAACTGGATTCGTGATTACCGCA
AAGAATGCCAAGCAACACCCGAAGCAATGAGTGAACTTGAGTTAATGGAAGAGAATCGTAAACTCAAACAAAAACTCGCTGAATTCGAAAAGAAAATGAC
TTTTTAAAAAAGGCAGCAGCATTCTTCGCGAAGGAAACAGGCAAGTAATCTACAAATTTATTGATCAGCATAAGAGCCACTTCGGCTTACGTTGGTTGTT
AAAACGATGTGGTGTTTATGCCAACGGCTATTATAACTATCTCAGAAATACAAAACGGGATTATCATCAAAGCAAGCAAGCTATCAAAAATAAAATCAAA
GATATCTTTCATTCCACTGGCGGCATTGTATGTCATCGTGGAATGGTCATATTCTTAGCTCGTGATGACATTAAGCTCAGTAAGACTACCGTTCATAAAT
ATATGAATAAAGAGCTACAACTCTACTGTATCCCCGCTAAGAAGCGCCCACGATACGTTGAAGGAACGAAACATGAGATATTCCCCAATCACTTATCGCA
AGACTTCGATATTGAAGAAAAGAACACAGTTTGGTGTACCGACTTTACTTATATCAAAGGGTGGGATGGCAAGTTCCAATACAACTGCAGTATTTTAGAC
CTTTCGGATCGGAGTATTGTCGCCACTAAAACAAGTCGAATGATGACAAGTGAACTAGCCAAAGAAACTTTAGAAACAGCACTATCATCACAGAAGAAAC
GACCTAAAGGATTGATTCTTCATAGTGATCAAGGCAGCCAATTCGCATCATATGATTTCACAGAATACTGTCTAAAAAACAATATAAAGCAGAGTATGAG
TAAAGCCGGATGTCCCTATGATAACGCAGTGATGGAACGATTTTTTAGAACCATGAAGTTTGAACTCATCAATCGATTCCAATTCGACAATGACAAGCAA
CTTGAAACTGCAATATCAGAATATGTATTTGGTTGGTATAACCAGGTAAGACCGCATGCATCAAATGGATATAAAACCCCAAACGAAATTAGAAACACTT
AGATCTATTTATACACTAGTGTTCCAAAAACGCTTGACCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 243 bp | 80 aa | 65 | 307 | + | No |
Description : First part of the transposase
ORF sequence :
MKRYSTEMKKKIVTANIQDGRTIASLATEYGVSTASINNWIRDYRKECQATPEAMSELELMEENRKLKQKLAEFEKKMTF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 903 bp | 300 aa | 300 | 1202 | + | No |
Description : Second part of the transposase
ORF sequence :
LFKKGSSILREGNRQVIYKFIDQHKSHFGLRWLLKRCGVYANGYYNYLRNTKRDYHQSKQAIKNKIKDIFHSTGGIVCHRGMVIFLARDDIKLSKTTVHK
YMNKELQLYCIPAKKRPRYVEGTKHEIFPNHLSQDFDIEEKNTVWCTDFTYIKGWDGKFQYNCSILDLSDRSIVATKTSRMMTSELAKETLETALSSQKK
RPKGLILHSDQGSQFASYDFTEYCLKNNIKQSMSKAGCPYDNAVMERFFRTMKFELINRFQFDNDKQLETAISEYVFGWYNQVRPHASNGYKTPNEIRNT
YMNKELQLYCIPAKKRPRYVEGTKHEIFPNHLSQDFDIEEKNTVWCTDFTYIKGWDGKFQYNCSILDLSDRSIVATKTSRMMTSELAKETLETALSSQKK
RPKGLILHSDQGSQFASYDFTEYCLKNNIKQSMSKAGCPYDNAVMERFFRTMKFELINRFQFDNDKQLETAISEYVFGWYNQVRPHASNGYKTPNEIRNT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1138 bp | 378 aa | 65 | 1202 | + | Yes |
Chemistry : DDE
ORF sequence :
MKRYSTEMKKKIVTANIQDGRTIASLATEYGVSTASINNWIRDYRKECQATPEAMSELELMEENRKLKQKLAEFEKKMTFKKGSSILREGNRQVIYKFID
QHKSHFGLRWLLKRCGVYANGYYNYLRNTKRDYHQSKQAIKNKIKDIFHSTGGIVCHRGMVIFLARDDIKLSKTTVHKYMNKELQLYCIPAKKRPRYVEG
TKHEIFPNHLSQDFDIEEKNTVWCTDFTYIKGWDGKFQYNCSILDLSDRSIVATKTSRMMTSELAKETLETALSSQKKRPKGLILHSDQGSQFASYDFTE
YCLKNNIKQSMSKAGCPYDNAVMERFFRTMKFELINRFQFDNDKQLETAISEYVFGWYNQVRPHASNGYKTPNEIRNT
QHKSHFGLRWLLKRCGVYANGYYNYLRNTKRDYHQSKQAIKNKIKDIFHSTGGIVCHRGMVIFLARDDIKLSKTTVHKYMNKELQLYCIPAKKRPRYVEG
TKHEIFPNHLSQDFDIEEKNTVWCTDFTYIKGWDGKFQYNCSILDLSDRSIVATKTSRMMTSELAKETLETALSSQKKRPKGLILHSDQGSQFASYDFTE
YCLKNNIKQSMSKAGCPYDNAVMERFFRTMKFELINRFQFDNDKQLETAISEYVFGWYNQVRPHASNGYKTPNEIRNT
Blast result :
Comments
ISErh6 is 68% aa similar to ISRgn1.
References
1] Yohsuke Ogawa, Yoshihiro Shimoji (2010) Direct submission.